Activities of antioxidant defense enzyme in some tissues of spinycheek crayfish (Orconectes limosus Rafinesque) from Serbian part of Danube river
2005
Authors:
Borković-Mitić, SlavicaMilošević, Slaviša
Radovanović, Tijana
Pavlović, Slađan
Štajn, Andraš Š.
Žikić, Radoslav V.
Maksimović-Kosanović, Katica
Saičić, Zorica
Contributors
Đurić, DraganJakovljević, Vladimir
Document Type:
Conference object (Published version)
,
© 2005 by the Serbian Physiology Society
Metadata
Show full item recordAbstract:
In our experiment we studied the antioxidant enzyme activities (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GSH-Px, glutathione reductase-GR, and glutathione-S-transferase-GST} in hepatopancreas, gills and abdominal muscle of spinycheek crayfish (Orconectes limosus) from the Serbian part of Danube River. The speciments were collected with deep nets and tissue samples were prepared for analisys by standard methods. All enzyme activities were measured spectrophotometrically and expressed as specific (U/mg proteins) and total (U/g net mass). The results show strong tissue specifity of investigated enzymes. Specific and total SOD activity in hepatopancreas was lower than in gills and muscle. At the same time, total SOD activity in gills was significantly lower in respect to muscle. The specific and total activity of CAT was significantly higher in hepatopancreas than in gills and muscle, as well as in gills in respect to the muscle. Same trend was obtained for specific and total activity of GSH-Px. In gills of spinycheek crayfish·, specific activity of GR was significantly higher than in hepatopancreas and muscle, as well as in gills in respect to the muscle. Total GR activity was also significantly higher in gills comparing to hepatopancreas and muscle. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. The obtained results show a strong tissue specifity of antioxidant defense enzymes in investigated tissues of spinycheek crayfish which is accompanied with its different metabolic activity.
Keywords:
Orconectes limosus; Antioxidant defense enzymes; Danube riverFunding / projects:
- Proučavanje poremećaja homeostaze i određivanje biomarkera oksidacionog stresa kod aerobnih organizama (RS-MESTD-MPN2006-2010-143035)
In:
- Đurić D, Jakovljević V, editors. Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. Belgrade: Serbian Physiology Society; 2005. p. 219.