Concentrations of vitamin E and sulphydryl group in some tissues of freshwater crayfish species
2005
Аутори:
Radovanović, TijanaMilošević, Slaviša
Pavlović, Slađan
Borković-Mitić, Slavica
Radojičić, Ratko
Žikić, Radoslav V.
Saičić, Zorica
Остала ауторства
Đurić, DraganJakovljević, Vladimir
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2005 by the Serbian Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
The freshwater crayfishes can be useful organisms in the biomonitoring of environmental conditions. In our experiments we studied vitamin E (Vit E) and sulphydryl group (-SH) concentrations in hepatopancreas, gills and abdominal muscle of three crayfish species: Noble crayfish (Astacus astacus) from South Morava river, Stone crayfish (Austropotamobius torrentium) from Krajkovacka river and Spinycheek crayfish (Orconectes limosus) from Danube river. All species were collected in summer season (August). The speciments were collected by deep nets and supematants for analysis were prepared according to the standard methods. Vit E concentration was assayed by standard method using bathophenantroline as reagent, while -SH groups concentration was measured by standard spectrophotometric method using dithionitrobenzoic acid (DTNB). The obtained results show significantly lower concentration of vit E in hepatopancreas of Orconectes limosus in respect to Astacus astacus (p<0.05). At the same time, significantly decreased concentration of vit E was detected in gills of Orconectes limosus comparing to Astacus astacus and Austropotamobius torrentium (p<0.005). Concentration of -SH groups was significantly increased in hepatopancreas of Noble crayfish in comparison to Stone crayfish (p<0.05) and Spinycheek crayfish (p<0.005). In conclusion, the concentrations of the investigated antioxidant compounds (vit E and
-SH groups) in the selected tissues of freshwater crayfishes are good markers for biomonitoring of environmental conditions. Differences in the investigated nonenzymatic oxidative stress parameters in tissues reflect different metabolic and antioxidative activities, which can be the consequence of species or tissue specifity, as well as the consequence of different environmental conditions.
Кључне речи:
Vitamin E; SH groups; CrayfishФинансирање / пројекти:
- Проучавање поремећаја хомеостазе и одређивање биомаркера оксидационог стреса код аеробних организама (RS-MESTD-MPN2006-2010-143035)
У:
- Đurić D, Jakovljević V, editors. Abstract Book: The first congress of physiological sciences of Serbia and Montenegro with international participation: Molecular, Cellular and Integrative Basis of Health, Disease and Therapy; 2005 Nov 9-12; Belgrade, Serbia. Belgrade: Serbian Physiology Society; 2005. p. 190.