Osmotically activated anion current of Phycomyces blakesleeanus-filamentous fungi counterpart to vertebrate volume regulated anion current
2023
Autori:
Stevanović, KatarinaČepkenović, Bogdana
Križak, Strahinja
Živić, Miroslav
Todorović, Nataša
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Studies of ion currents in filamentous fungi are a prerequisite for forming a complete
understanding of their physiology. Cytoplasmic droplets (CDs), obtained from sporangiophores of
Phycomyces blakesleeanus, are a model system that enables the characterization of ion currents in
the native membrane, including the currents mediated by the channels not yet molecularly identified.
Osmotically activated anionic current with outward rectification (ORIC) is a dominant current in
the membrane of cytoplasmic droplets under the conditions of hypoosmotic stimulation. We have
previously reported remarkable functional similarities of ORIC with the vertebrate volume regulated
anionic current (VRAC), such as dose-dependent activation by osmotic difference, ion selectivity
sequence, and time and voltage dependent profile of the current. Using the patch clamp method on
the CD membrane, we further resolve VRAC-like ORIC characteristics in this paper. We examine
the inhibition by extracellular ATP and carbenoxolone, the permeation of glutamate in presence of
chloride, selectivity for nitrates, and activation by GTP, and we show its single channel behavior
in excised membrane. We propose that ORIC is a functional counterpart of vertebrate VRAC in
filamentous fungi, possibly with a similar essential role in anion efflux during cell volume regulation.
Ključne reči:
osmotic stress; ATP; nitrate; glutamate; patch-clamp; single channel currentIzvor:
Journal of Fungi, 2023, 9, 6, 637-Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/jof9060637
ISSN: 2309-608X
PubMed: 37367573