Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats
2018
Authors:
Potrebić, MilicaAjdžanović, Vladimir
Miler, Marko
Milenković, Dragan
Pendoski, Laze
Jakovljević, Vladimir
Milošević, Verica
Document Type:
Conference object (Published version)
,
© 2018 by the EndoScience Endokrinologie Service GmbH
Metadata
Show full item recordAbstract:
Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.
Funding / projects:
- The effects of select plant extracts, phytoestrogens, steroid and peptide hormones on the rat neuroendocrine system (RS-MESTD-Basic Research (BR or ON)-173009)
In:
- 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland. Altdorf: EndoScience Endokrinologie Service GmbH; 2018. p. 27-8.