Combining metabolomic and transcriptomic approaches to decipher the diversity of iridoids within the genus Nepeta (fam. Lamiaceae)
2023
Аутори:
Mišić, DanijelaAničić, Neda
Matekalo, Dragana
Gašić, Uroš
Skorić, Marijana
Šiler, Branislav
Banjanac, Tijana
Nestorović Živković, Jasmina
Dmitrović, Slavica
Filipović, Biljana
Milutinović, Milica
Božunović, Jelena
Petrović, Luka
Todorović, Miloš
Lukić, Tamara
Остала ауторства
Peters, ReubenZerbe, Philipp
Tantillo, Dean
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by the University of California, Davis
Метаподаци
Приказ свих података о документуАпстракт:
Data on the distribution and diversity of iridoid glycosides (IGs) and iridoid aglycones (IAs) across the genus Nepeta (fam. Lamiaceae) are fragmentary; only rarely these two subgroups of iridoids are simultaneously analysed. Our ambition was to fulfil these knowledge gaps by adopting state-of-the-art metabolomics techniques (untargeted LC-HRMS and GC/MS) to comprehensively acquire, analyse and interpret the overall diversity of iridoids in selected phyllo-diverse Nepeta taxa, and thus facilitate further reconstruction of the molecular background of this diversity. Analyzed taxa were robustly grouped into three major chemotypes: (A) producers of both IGs and IAs; (B) taxa exclusively producing IGs; and (C) iridoid-lacking taxa. Furthermore, Nepeta species belonging to the chemotype A displayed significant heterogeneity in their IAs composition, and were roughly classified into several sub-chemotypes based on the stereochemistry of the major nepetalactone. No clear regularities between chemotype affiliation and phylogenetic relatedness, reconstructed adopting DNA barcoding tools, was revealed. Transcriptomes of leaves of distinguished Nepeta chemotypes were mined for the transcripts of iridoid-related biosynthetic genes, to facilitate the assessment of the factors responsible for the presence/absence of iridoids, or specific subgroups of iridoids, in these plants. Integration of metabolomics and transcriptomics data pointed to several key genes in the biosynthetic pathway as significant determinants of the iridoid diversity within the genus: geraniol synthase (GES), iridoid synthase (ISY), NAD-dependent nepetalactol-related short-chain-dehydrogenases/reductases (NEPSs), and latex protein-like genes (MLPLs). This study provided a framework to revisit some of the iridoid-metabolism-related ecological and chemical evolution theories, which is the course of our further work.
Кључне речи:
Nepeta; catmint; catnip; iridoids; transcriptomics; metabolomicsФинансирање / пројекти:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Peters R, Zerbe P, Tantillo D, editors. Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis, Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. Davis: University of California; 2023. p. 100.