Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
2016
Autori:
Prodanović, RadišaKorićanac, Goran
Vujanac, Ivan
Đorđević, Ana
Pantelić, Marija
Romić, Snježana
Stanimirović, Zoran
Kirovski, Danijela
Tip dokumenta:
Članak u časopisu (Nerecenzirana verzija)
,
© 2016 by the authors
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepaticfatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groupsbased on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenousglucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collectedbefore (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levelswere determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measuredin the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptorbeta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglyceridesand hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The developmentof insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression ofthe IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevantin the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
Napomena:
This is a submitted version of the following article: Prodanović R, Korićanac G, Vujanac I, Djordjević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. Res Vet Sci. 2016;107:16–9. http://dx.doi.org/10.1016/j.rvsc.2016.04.007.
Ključne reči:
dairy cows; fatty liver; CD36; Sterol regulatory element-binding protein-1Izvor:
Research in Veterinary Science, 2016, 107, 16-19Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Uloga steroidnih hormona u neuroendokrinoj adaptaciji na stres i patofiziologiji metaboličkog sindroma - molekularni mehanizmi i kliničke implikacije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
Povezane informacije:
- Druga verzija
https://radar.ibiss.bg.ac.rs/handle/123456789/6085
DOI: 10.1016/j.rvsc.2016.04.007
ISSN: 0034-5288
PubMed: 27473969