Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats
2014
Аутори:
Šošić-Jurjević, BrankaFilipović, Branko
Wirth, Eva Katrin
Živanović, Jasmina
Radulović, Niko
Janković, Snežana
Milošević, Verica
Köhrle, Josef
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2014 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh beta, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T-3-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T-4 bound to Tg, determined thyroid T-4 levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T-4 levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats.
Кључне речи:
isoflavones; genistein; daidzein; thyroid homeostasis; middle age; ratИзвор:
Toxicology and Applied Pharmacology, 2014, 278, 2, 124-134Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.1016/j.taap.2014.04.018
ISSN: 0041-008X
PubMed: 24793811