Germacrene A synthase and oxidase promoter analysis in chicory
2015
Authors:
Bogdanović, MilicaDragićević, Milan
Cankar, Katarina
Beekwilder, Jules
Bouwmeester, Harro
Subotić, Angelina
Simonović, Ana
Todorović, Slađana
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2015 the Serbian Biological Society
Metadata
Show full item recordAbstract:
Chicory (Cichorium intybus L.) is rich in sesquiterpene lactones, bitter compounds whose biosynthesis involves several recently characterized genes, namely germacrene A synthase (GAS), in chicory present as long and short form, and germacrene A oxidase (GAO, CYP71AV8). Promoters of these genes have been studied by cloning promoter regions to drive the expression of eGFP, and obtaining stable chicory transformants carrying promoter constructs. Due to incompatibility of eGFP with another fluorescent marker – DsRED, which was used for selection of transformed clones, promoter activity was detected in transgenic plants by RT-PCR and qRT-PCR techniques. Most of the obtained chicory clones were expressing GFP, in roots, leaves, stems and flowers, suggesting that cloned promoters were functional in different chicory organs. The promoters were characterized by different strength - GAO and GAS long promoters were stronger than both GAS short promoters, judging by eGFP expression level. The promoters also showed partial tissue specificity – GAS long was active in roots, leaves, stems and flowers, while GAS short promoters were mainly active in chicory roots.
Keywords:
germacrene A synthase; germacrene A oxidase; promoter analysis; eGFPFunding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Plant Terpenoids for Human Health: a chemical and genomic approach to identify and produce bioactive compounds (EU-FP7-227448)
In:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 19.