DUF1070 is a conserved signature domain of some arabinogalactan peptides
2015
Autori:
Simonović, AnaDragićević, Milan
Filipović, Biljana
Todorović, Slađana
Bogdanović, Milica
Trifunović-Momčilov, Milana
Subotić, Angelina
Ostala autorstva
Uzelac, BrankaTip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2015 the Serbian Biological Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Most proteins are composed of functional domains, of which >20% are “domains of unknown function” (DUFs). We have recently identified an arabinogalactan (AG) peptide (AGN92423) with DUF1070 domain in Centaurium erythraea transcriptome. AGPs are ubiquitous plant cell wall hydroxyproline-rich glycoproteins with diverse functions. Since classical AGPs and AG peptides contain no signature motifs, the presence of highly conserved DUF1070 caught our attention. DUF1070 is present in 479 NCBI plant protein sequences, of which 271 are non-redundant: 126 AG peptides and 145 unknown/hypothetical proteins. All DUF1070-containing sequences are short (71 aa on average). With few exceptions, these sequences are comprised of N-terminal signal sequence, followed by an AG-II glycomodule (typically PAPAPT). DUF1070 is up to 37 aa long, encompassing (a part of) AG-II glycomodule and a highly conserved C-terminal stretch of 26 aa, typically “SDGTSIDQGIAYVLMLVALVLTYLIH”. This motif is a textbook example of glycosylphosphatidylinositol (GPI) anchor signal peptide, containing 1) three relatively small aliphatic amino acids at the ω (GPI attachment site), ω+1 and ω+2 positions (SDG in 74% sequences); 2) a relatively polar spacer (often TSIDQG), followed by 3) a hydrophobic domain (typically IAYVLMLVALVLTYLIH). When GPI is attached to ω (S), the C-terminal peptide is cleaved, while the hydrophobic domain remains embedded in the ER membrane. Since most of the DUF1070 is predicted to be cleaved and discarded, it is worth wondering what selective pressure kept it so conserved (unlike other GPIsp sequences) among AG peptides from unrelated plant species. We propose that DUF1070 is a signature of some AG peptides.
Ključne reči:
arabinogalactan proteins; AG peptides; DUF1070; glycosylphosphatidylinositol anchor; GPI signal peptideFinansiranje / projekti:
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)
- Razvoj i primena biotehnoloških postupaka u dobijanju zdravog sadnog materijala ukrasnih biljaka (RS-MESTD-Technological Development (TD or TR)-31019)
U:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 40-1.