The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
2023
Autori:
Popović, DušankaMirkov, Ivana
Popov Aleksandrov, Aleksandra
Tucović, Dina
Kulaš, Jelena
Malešević, Anastasija
Glamočlija, Jasmina
Soković Bajić, Svetlana
Brdarić, Emilija
Tolinački, Maja
Golić, Nataša
Ostala autorstva
Capurso, LucioGuarino, Alfredo
Gasbarrini, Antonio
Morelli, Lorenzo
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
,
© 2023 EMEC, Roma
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
Ključne reči:
Aspergillus fumigatus; lung infection; gut microbita; rats; inflammationFinansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
U:
- Capurso L, Guarino A, Gasbarrini A, Morelli L, editors. 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023 Sep 16-19; Rome, Italy. Roma: EMEC; 2023. p. 90.