The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils
2015
Authors:
Dmitrović, SlavicaPerišić, Mirjana
Stojić, Andreja
Živković, Suzana
Božunović, Jelena
Nestorović Živković, Jasmina
Aničić, Neda
Ristić, Mihailo
Mišić, Danijela
Contributors
Uzelac, BrankaDocument Type:
Conference object (Published version)
,
© 2015 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.
Keywords:
Ambrosia artemisiifolia; nepetalactone; antioxidative enzymes; Nepeta rtanjensis; Nepeta catariaFunding / projects:
- Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
In:
- Uzelac B, editor. Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”; 2015. p. 159.