Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation
2016
Autori:
Hmaid, Amal AAAMarkelić, Milica
Otašević, Vesna
Mašović, Sava
Janković, Aleksandra
Korać, Bato
Korać, Aleksandra
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Structural changes affecting cardiomyocyte function may contribute to the pathophysiological remodeling underlying cardiac function impairment. Recent reports have shown that endogenous nitric oxide (NO) plays an important role in this process. In order to examine the role of NO in cardiomyocyte remodeling, male rats were acclimated to room temperature (22 ± 1 °C) or cold (4 ± 1 °C) and treated with 2.25% l-arginine·HCl or 0.01% l-NAME (Nω-nitro-l-arginine methyl ester)·HCl for 45 days. Untreated groups served as controls. Right heart ventricles were routinely prepared for light microscopic examination. Stereological estimations of volume densities of cardiomyocytes, surrounding blood vessels and connective tissue, as well as the morphometric measurements of cardiomyocyte diameters were performed. Tissue sections were also analyzed for structural alterations. We observed that both l-arginine and l-NAME supplementation induced cardiomyocyte hypertrophy, regardless of ambient temperature. However, cardiomyocyte hypertrophy was associated with fibrosis and extra collagen deposition only in the l-NAME treated group. Taken together, our results suggest that NO has a modulatory role in right heart ventricle remodeling by coordinating hypertrophy of cardiomyocytes and fibrous tissue preventing cardiac fibrosis.
Ključne reči:
Cardiac hypertrophy; Cardiomyocyte; Myocardium; l-Arginine; L-NAMEIzvor:
Saudi Journal of Biological Sciences, 2016, 25, 3, 537-544Finansiranje / projekti:
- Belo ili/i mrko: značaj masnog tkiva u održanju ukupne redoks zavisne metaboličke kontrole u fiziološkim adaptacijama i metaboličkim poremećajima (RS-MESTD-Basic Research (BR or ON)-173055)
DOI: 10.1016/j.sjbs.2016.01.022
ISSN: 1319-562X
PubMed: 29686516