Faecal pollution and antibiotic resistance in the Danube River: Vision, Concepts and Lessons learned from Joint Danube Surveys
2022
Autori:
Kirschner, Alexander K.T.Schachner, Iris
Jakwerth, Stefan
Zarfel, Gernot
Koller, Michael
Kittinger, Clemens
Tóth, Erika
Kolarević, Stoimir
Kračun-Kolarević, Margareta
Blaschke, Alfred Paul
Derx, Julia
Linke, Rita
Demeter, Katalin
Reischer, Georg
Savio, Domenico
Farnleitner, Andreas H.
Tip dokumenta:
Konferencijski prilog (Objavljena verzija)
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© 2022 by the Serbian Society of Microbiology
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Sustainable and target-oriented microbiological water quality management of rivers needs information on
whole river systems, especially if catchments are large and international. The microbiological water quality
of rivers is of uttermost relevance for human health as river water is used for several purposes (recreation,
drinking water production, irrigation). Besides information on faecal pollution levels, the origin of faecal
pollution and the assessment of associated infection- and health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and discusses future challenges and perspectives (pollution-scenario modelling, infection-risk assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS. Beside standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput amplicon-sequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big picture”
of the microbiological pollution status of large rivers. Harmonised trans-border microbiological water quality aps for the whole navigable Danube were established. MST marker analysis elucidated that the main faecal pollution source along the whole river is human wastewater. Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the potential health impacts of AMR associated with faecal pollution.
Finansiranje / projekti:
- Austrian Science Fund FWF (P25817, P32464)
U:
- Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. Belgrade: Serbian Society of Microbiology; 2022. p. 144-5.