Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
2013
Authors:
Vukašinović, ElviraPond, David
Worland, Michael Roger
Kojić, Danijela
Purać, Jelena
Blagojević, Duško
Grubor-Lajšić, Gordana
Document Type:
Article (Published version)
,
© 2013 Elsevier Inc.
Metadata
Show full item recordAbstract:
This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
Keywords:
Insect diapause; Cold hardiness; Ostrinia nubilalis; Lipid classes and fatty acid composition; Phase transition temperatures of lipidsSource:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2013, 165, 4, 219-225Funding / projects:
- Molecular mechanisms of redox signalling in homeostasis: adaptation and pathology (RS-MESTD-Basic Research (BR or ON)-173014)
- Ministry of Education, Youth & Sports - Czech Republic
- International Visegrad Scholarship Fund (Bratislava, Slovakia)
DOI: 10.1016/j.cbpb.2013.05.001
ISSN: 1096-4959
PubMed: 23701990