Molecular markers in the detection of interspecies hybridization – a model study on the genus Centaurium Hill
2023
Authors:
Lukić, TamaraŠiler, Branislav
Mišić, Danijela
Nestorović Živković, Jasmina
Jelić, Mihailo
Brkušanin, Miloš
Filipović, Biljana
Todorović, Miloš
Banjanac, Tijana
Document Type:
Conference object (Published version)
,
© 2023 by the French Society of Plant Biology
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Show full item recordAbstract:
The species of the genus Centaurium Hill are characterized by a pronounced phenotypic
plasticity, while interspecific hybridization readily occurs. Previous studies reported hybridization events between C. erythraea and C. tenuiflorum in the Mediterranean region,
but the status of a potentially hybrid taxon (termed "Centaurium ×tenuiflorum") remains
unclear. Therefore, molecular markers (EST-SSR, ITS, and chloroplast DNA markers) were
combined and their usefulness in estimating overall genetic variability and in identifying
phylogenetic relations between Centaurium taxa was scrutinized. Both EST-SSR and ITS
markers proved to be well suited to distinguish the parental taxa C. erythraea and C.
tenuiflorum, and a putative hybridogenic taxon "C. ×tenuiflorum", the last one being more
related to C. erythraea. Conversely, maternally inherited chloroplast markers (trnL-F, rbcL
and psbA-trnH) nominated C. tenuiflorum as a donor of plastid.
Keywords:
Centaurium; hybridization; EST-SSR; ITS; trnL-trnF; trnH-psbA; rbcLFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. French Society of Plant Biology; 2023. p. 79-80.