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Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model
dc.creator | Radulović, Nataša | |
dc.creator | Pilipović, Ivan | |
dc.creator | Stojanović, Ivana D. | |
dc.date.accessioned | 2024-01-12T14:30:51Z | |
dc.date.available | 2024-01-12T14:30:51Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6472 | |
dc.description.abstract | Cyclophosphamide (CP) is a cytostatic, widely used to treat different carcinomas and autoimmune diseases. It is commonly used in experimental designs modeling immunosuppression in laboratory animals, with different approaches for CP treatment but without a consensus on the dose, timing, and route of administration. We aimed to establish if treatment with CP in C57BL/6 mice depletes regulatory T cells (Tregs). Tregs are a crucial component of the immune system that helps maintain immune tolerance and prevent excessive immune reactions. They are significant in autoimmune diseases, allergies, and immune-related therapies. CP was applied intraperitoneally (i.p.) twice in a 5-day interval in doses of 100 mg/ kg. Monitoring of Treg prevalence in peripheral blood after each treatment and in the spleen after the second treatment with CP revealed a drop in the number of Tregs after two doses of CP because of the decreased number of total lymphocytes but not as a specific response of the Tregs. The prevalence of Tregs in peripheral blood after CP treatment mirrored the change in Treg number in the spleen. CP treatment induced a decrease in the number of CD3+ cells in the spleen while increasing their proportion, indicating that CP affected the B lymphocyte population rather than T cells. Our results suggest that CP treatment cannot be used as a specific Treg-depleting agent in the C57BL/6 animal model. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade: Serbian Biological Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Archive of Biological Sciences | sr |
dc.subject | cyclophosphamide | sr |
dc.subject | immunosuppression | sr |
dc.subject | regulatory T cells | sr |
dc.subject | lymphocytes | sr |
dc.subject | mouse experimental model | sr |
dc.title | Application of an intermediate concentration of cyclophosphamide does not specifically deplete regulatory T cells in a mouse experimental model | sr |
dc.type | article | sr |
dc.rights.license | BY | sr |
dc.rights.holder | © 2023 by the authors | sr |
dc.citation.issue | 4 | |
dc.citation.volume | 75 | |
dc.identifier.doi | 10.2298/ABS230715032R | |
dc.identifier.wos | 001125519500004 | |
dc.citation.spage | 397 | |
dc.citation.epage | 406 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/16555/bitstream_16555.pdf | |
dc.citation.rank | M23 |