Interpreting the chemodiversity in a phylogenetic context: a case study of the genus Nepeta
2023
Authors:
Banjanac, TijanaŠiler, Branislav
Lukić, Tamara
Gašić, Uroš
Petrović, Luka
Matekalo, Dragana
Skorić, Marijana
Nestorović Živković, Jasmina
Dmitrović, Slavica
Milutinović, Milica
Aničić, Neda
Božunović, Jelena
Filipović, Biljana
Todorović, Miloš
Mišić, Danijela
Document Type:
Conference object (Published version)
,
© 2023 by the French Society of Plant Biology
Metadata
Show full item recordAbstract:
Nepeta L. is the largest genus of the Lamiaceae family. The species of this genus are famous
for their biopesticidal potential and medicinal properties. Within the genus, we found
considerable variability in the qualitative and quantitative content of iridoid and phenolic
compounds. Using state-of-the-art metabolomics techniques (UHPLC/LTQ-Orbitrap MS,
GC/MS, UHPLC/DAD/(+/-)HESI-MS2), we comprehensively assessed the overall diversity of iridoids and phenolics in the selected chemodiverse Nepeta taxa at the intra-species level.
To reconstruct phylogenetic relations among the selected chemodiverse taxa from across the
Nepeta genus, genomic ITS molecular marker and two maternally inherited plastid DNA
regions (trnL-trnF and rbcL) were analyzed. A consensus phylogenetic tree was constructed
to interpret the distribution of the most abundant iridoid and phenolic compounds. It was
not possible to uncover clear regularities between phylogenetic relationship and chemotype
affiliation.
Keywords:
Nepeta; UHPLC/LTQ-Orbitrap MS; GC/MS; UHPLC/DAD/(+/-)HESI-MS 2; trnL-trnF; rbcL; ITSFunding / projects:
- NEPETOME - Omics-guided disentangling of the iridoid diversity in the genus Nepeta L. for in planta metabolic engineering towards sustainable exploitation (RS-ScienceFundRS-Ideje-7749433)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- 14th International Conference of the French Society of Plant Biology; 2023 Jul 3-6; Marseille, France. French Society of Plant Biology; 2023. p. 78-9.