Ispitivanje uticaja ekstrakta vlašca (Allium schoenoprasum L.) i kvercetina na morfološke i fiziološke karakteristike klijanaca Arabidopsis thaliana
Assessment of the effect of chive extract (Allium schoenoprasum L.) and quercetin on morphological and physological characteristics of Arabidopsis thaliana seedlings
2022
Document Type:
Conference object (Published version)
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© 2022 Naučno-istraživačko društvo studenata biologije i ekologije „Josif Pančić
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Show full item recordAbstract:
Chive leaf extract is a rich source of flavonols (kaempferol, quercetin and izorahmentin) and flavonoid glycosides (kaempferol-3-glucoside and quercetin-3-glucoside). These compounds are a very important group of flavonoids that have a broad range of biological activities. The aim of this study is to investigate the response of Arabidopsis thaliana to the presence of chive extract (concentration of 100 mg/L) and quercetin (concentration of 15.11 mg/L) in the growth medium. The length of hypocotyl was measured on the 5th, 7th, and 10th day after the germination of seeds. The presence of quercetin in the growth medium significantly effects the elongation of the hypocotyl. Accumulation of H2O2 in the plant tissue of seedlings was detected by DAB staining (3,3’-diami nobenzidine). These results correspond with increased activity of antioxidative enzyme peroxidase in seedlings that grow on the medium with chive extract. The activity of glutathione transferase was also determined. This enzyme is predominantly located in the cytosol and participates in the process of detoxification of plant cells. The described changes in antioxidative enzyme activities in plant model seedlings on growth medium with chive extract guide us towards new steps in research with the possible development of a potential bioherbicide
Keywords:
ekstrakt; kvercetin; vlašac; Arabidopsis thaliana; hipokotil; peroksidaze; DAB; glutation transferazaFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Bailović B, Vukčević A, editors. Knjiga izvoda: 4. Konferencija studenata biologije, ekologije i zaštite životne sredine: Ekobiomorfa; 2022 Nov 25-27; Novi Sad, Serbia. Novi Sad: Naučno-istraživačko društvo studenata biologije i ekologije „Josif Pančić“; 2022. p. 12-3.