The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease
2024
Authors:
Perović, MilkaĆirić, Jelena
Matović, Valentina
Srbovan, Maja
Koruga, Đuro
Kanazir, Selma
Ivković, Sanja
Document Type:
Article (Accepted Version)
Metadata
Show full item recordAbstract:
Introduction: In the present study, we assessed the effects of the hyper- harmonized-
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis
through machine learning (ML) using artificial neural networks (ANNs) to classify the
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in
cortical and hippocampal tissue were assessed.
Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not
induce the activation of glia (astrocytes and microglia) nor did it negatively affect
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
Conclusion: The obtained results point to the potential of 3HFWC, when applied in
the presymptomatic phase of AD, to interfere with amyloid plaque formation without
inducing AD- related pathological processes such as neuroinflammation, gliosis, and
synaptic vulnerability.
Keywords:
3HFWC; 5XFAD mice; Alzheimer's disease; cortex; machine learning; near- infrared spectroscopy; preventionSource:
CNS Neuroscience and Therapeutics, 2024, 30, 3, e14188-Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Zepter International foundation (project No. #5/2019)
DOI: 10.1111/cns.14188
ISSN: 1755-5930
PubMed: 36971205