Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves
2022
Authors:
Marković Hadžić, StefanBožunović, Jelena
Marković, Tijana
Aničić, Neda
Gašić, Uroš
Mišić, Danijela
Contributors
Milutinović, MilicaDocument Type:
Conference object (Published version)
,
© 2022 by the Serbian Plant Physiology Society
Metadata
Show full item recordAbstract:
Studies focusing on plant specialized metabolism and enhanced production of bioactive compounds in a controlled environment are of increasing interest bearing in mind that some of those compounds are widely used. Centaurium erythraea Rafn produces a plethora of specialized metabolites among which secoiridoid glucosides predominate. Secoiridoids in plants serve as defense compounds against herbivores and microorganisms, which makes them highly attractive
for agricultural and pharmaceutical applications. In plants, ambient UV–B radiation has been suggested to prime protective responses towards various stressors. The overall goal of our research
was to reveal the effect of short-term UV–B light exposure (30 and 60 minutes) on secoiridoid
metabolism in leaves of common centaury grown in vitro. In order to achieve this goal, expression analysis of genes involved in the secoiridoid biosynthetic path-way was performed in parallel
with UHPLC/(–)HESI–MS2 metabolic profiling of leaves. Centaury plants exposed to a UV–B light
for 60 minutes displayed prominent discoloration, indicating possible chlorophyll degradation,
or its reduced synthesis. Gene expression analysis of nine secoiridoid biosynthesis-related genes
(CeGPPS, CeGES, CeG80, Ce8HGO, CeIS, CeIO, Ce7DGLT, Ce7DLH, CeSLS) was monitored. Following the UV–B treatment, a statistically significant decrease in relative gene expression was detected only for CeG80, while metabolic profiling revealed elevated accumulation of sweroside, swertiamarin,
and gentiopicrin in both 30 and 60 min UV–B-treated centaury plants. These results suggest that
UV–B light exposure can be used to stimulate accumulation of secoiridoids in centaury plants for
possible extraction and application in pharmacy, agriculture and food industry.
Keywords:
Centaurium erythraea; UV – B radiation; secoiridoid glucosidesFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
In:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 127.