Modulation of autophagy by SARS-CoV-2 proteins
Модулација аутофагије SARS-CoV-2 протеинима
2022
Authors:
Paunović, VericaMisirkić Marjanović, Maja
Vučićević, Ljubica
Stevanović, Danijela
Ristić, Biljana
Bošnjak, Mihajlo
Mandić, Miloš
Trajković, Vladimir
Contributors
Lalić, NebojšaČolić, Miodrag
Document Type:
Conference object (Published version)
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© Srpska akademija nauka i umetnosti 2022
Metadata
Show full item recordAbstract:
Autophagy is a homeostatic lysosome-dependent catabolic process that eliminates damaged organelles, dysfunctional proteins, and macromolecular aggregates. Autophagy plays an important role in host response to viral infection as it enables degradation of viruses in autophagolysosomes and regulates innate and adaptive immunity. However, some viruses, including SARS-CoV-2, have evolved a variety of mechanisms to avoid autophagic degradation and use it for their own benefit. The aim of this study is to investigate the impact of the individual SARS-CoV-2 proteins (M, E, N, NSP4, NSP5, NSP6, NSP7, NSP8, NSP10, NSP12, NSP14, and NSP15) on autophagy in human lung epithelial cells by analyzing the expression of autophagy-related proteins, LC3-II, p62, and beclin1. The immunoblot analysis revealed that intracellular expression of non-structural proteins NSP4, NSP6, and NSP8 increased the levels of autophagy markers LC3-II and beclin-1, while the structural N protein and non-structural proteins NSP5, NSP10, and NSP15, reduced the degradation of autophagy-selective target p62. These data indicate that some SARS-CoV-2 proteins induce autophagic response, while others block its completion, thus providing grounds for further investigation of the complex interaction between the virus and the autophagic pathway.
Keywords:
SARS-CoV-2; autophagy; lung epithelial cellsFunding / projects:
- TACTICIAN – Targeting Autophagy to Combat SARS-CoV2-induced Immune Dysregulation (RS-ScienceFundRS-Fond_2020_COVID19-7552006)
In:
- Lalić N, Čolić M, editors. Proceedings: COVID-19 Pandemic: Messages, New Information and Dilemmas; 2021 Jun 4; Belgrade, Serbia. Beograd: Srpska akademija nauka i umetnosti; 2022. p. 205-12.