Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects
2024
Authors:
Ninković, Slavica![](/themes/MirageIBISS/images/orcid.png)
Motyka, Václav
Stanišić, Mariana
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Smailagić, Dijana
![](/themes/MirageIBISS/images/orcid.png)
Živanović, Branka
Dobrev, Petre I.
Banjac, Nevena
![](/themes/MirageIBISS/images/orcid.png)
Document Type:
Article (Accepted Version)
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© 2024, The Author(s), under exclusive licence to Springer Science Business Media, LLC, part of Springer Nature
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Show full item recordAbstract:
Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research. Hairy root growth media of two different species, the fruit tree species Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.
Note:
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00344-024-11328-5
Keywords:
allelopathy; apple; Arabidopsis; Chenopodium murale; membrane potential; salicylic acidSource:
Journal of Plant Growth Regulation, 2024Funding / projects:
- Ministry of Education, Youth and Sports of the Czech Republic from European Regional Development Fund-Project “Centre for Experimental Plant Biology” (No. CZ.02.1.01/0.0/0.0/16_019/0000738).
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
Related info:
DOI: 10.1007/s00344-024-11328-5
ISSN: 0721-7595