Analysis of differentially methylated DNA sequences in potato plants exposed to French marigold essential oil
2024
Аутори:
Savić, JelenaMilutinović, Milica
Dragićević, Milan
Seničar, Mladen
Stupar, Sofija
Šajkunić, Sanja
Devrnja, Nina
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2024 by the European Cooperation in Science and Technology
Метаподаци
Приказ свих података о документуАпстракт:
Volatile organic compounds (VOCs) are important for tropospheric chemistry and
have diverse roles in ecosystems. Plants ‘sense’ VOCs to efficiently adapt and respond
to their environment. Volatiles of neighboring plants through alternations in the receiver
plant’s physiology contribute to the induction of direct plant defense against pest
organisms and elevate immunity status by translating plants to ‘primed’ status when
they are ready for rapid and energy-efficient response to upcoming attacks. Induced
epigenetic changes, on the level of DNA and histones, could be involved in this
response. Plant essential oils (EOs) have shown promising prospects as novel priming
inducers, but a little is known about their potential to induce previous epigenetic
alternations. In this study, potato (Solanum tuberosum L.) plants were exposed to
French marigold (Tagetes patula L.) EO during 3 consecutive days for 8h each day and
samples were collected 10 days after EO removal. Whole-Genome Bisulfite Sequencing
(WGBS) was used to identify EO-induced differentially methylated (DM) DNA
sequences. Average methylation level of whole genome and every chromosome,
percentage of cytosine methylation in CG, CHG and CHH contextes, number of
selected DM elements (promoters, exons, introns, 3‘UTR, 5‘UTR, and intergenic
regions), were analyzed at differential methylation ratio between control and treated
samples. Functional annotation of DM genes was done with KEGG pathway maps and
Gene Ontology categories, and associated DM genes were identified for upcoming
analyses of gene transcription in potato plants challenged with different abiotic and
biotic stress conditions.
Кључне речи:
DNA methylation; whole-genome bisulfite sequencing; essential oil; defense primingФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Book of Abstracts: 4th EPI-CATCH Conference Epigenetic Mechanisms of Crop Adaptations to Climate Change: Plant breeding in view of epigenetic mechanisms and technological advancements; Jun 4-6 2024; Novi Sad, Serbia. European Cooperation in Science and Technology; 2024.