In Vitro Biocompatibility Evaluation of Novel Urethane–Siloxane Co-Polymers Based on Poly(ϵ-Caprolactone)- block-Poly(Dimethylsiloxane)- block-Poly(ϵ-Caprolactone)
2012
Authors:
Pergal, Marija V.Antić, Vesna V.
Tovilović-Kovačević, Gordana
Brkljačić, Jelena
Vasiljević-Radović, Dana
Djonlagić, Jasna
Document Type:
Article (Accepted Version)
,
© 2011 by Taylor & Francis Ltd
Metadata
Show full item recordAbstract:
Novel polyurethane co-polymers (TPUs), based on poly(ε-caprolactone)-block-poly(dimethylsiloxane)-block-poly(ε-caprolactone) (PCL-PDMS-PCL) as soft segment and 4,4′-methylenediphenyl diisocyanate (MDI) and 1,4-butanediol (BD) as hard segment, were synthesized and evaluated for biomedical applications. The content of hard segments (HS) in the polymer chains was varied from 9 to 63 wt%. The influence of the content and length of the HS on the thermal, surface, mechanical properties and biocompatibility was investigated. The structure, composition and HS length were examined using 1H- and quantitative 13CNMR spectroscopy. DSC results implied that the synthesized TPUs were semicrystalline polymers in which both the hard MDI/BD and soft PCL-PDMS-PCL segments participated. It was found that an increase in the average HS length (from 1.2 to 14.4 MDI/BD units) was accompanied by an increase in the crystallinity of the hard segments, storage moduli, hydrophilicity and degree of microphase separation of the co-polymers. Depending on the HS content, a gradual variation in surface properties of co-polymers was revealed by FTIR, AFM and static water contact angle measurements. The in vitro biocompatibility of co-polymers was evaluated using the endothelial EA.hy926 cell line and protein adsorption on the polyurethane films. All synthesized TPUs adsorbed more albumin than fibrinogen from multicomponent protein mixture, which may indicate biocompatibility. The polyurethane films with high HS content and/or high roughness coefficient exhibit good surface properties and biocompatible behavior, which was confirmed by non-toxic effects to cells and good cell adhesion. Therefore, the non-cytotoxic chemistry of the co-polymers makes them good candidates for further development as biomedical implants.
Note:
This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition on 08 May 2012, available at: https://doi.org/10.1163%2F092050611X589338
Keywords:
Segmented polyurethanes; biocompatibility; surface properties; α,ω-dihydroxy-(PCL-PDMS-PCL); endothelial cellsSource:
Journal of Biomaterials Science: Polymer Edition, 2012, 23, 13, 1629-1657Funding / projects:
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.1163/092050611X589338
ISSN: 0920-5063
PubMed: 21888759