Acute-Phase-Dependent Binding Affinity of C/EBPβ from the Nuclear Extract and Nuclear Matrix towards the Hormone Response Element of the α2-Macroglobulin Gene in Rat Hepatocytes
2003
Аутори:
Bogojević, DesankaMihailović, Mirjana
Miodrag, Petrović
Dinić, Svetlana
Ivanović Matić, Svetlana
Poznanović, Goran
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2003 Institute of Molecular Physiology and Genetics, Centre of Biosciences, Slovak Academy of Sciences
Метаподаци
Приказ свих података о документуАпстракт:
Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied. By Western and South-Western blot analysis we have shown the presence of C/EBP beta in the examined nuclear fractions as well as its increased binding affinity to the examined gene sequence during the acute-phase response. The results suggest that both nuclear protein fractions could participate in the transcriptional regulation of the alpha2-macroglobulin gene in the rat hepatocytes.
Кључне речи:
C/EBPβ; α2-macroglobulin gene; Nuclear extract; Nuclear matrix; HepatocytesИзвор:
General Physiology and Biophysics, 2003, 22, 2, 279-285Финансирање / пројекти:
- the Research Science Fund of the Serbian Ministry of Science, Contract No. 1722.