TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan V
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1042
AB  - Gentiana dinarica Beck, native to the Balkan Dinaric Mountains, was established in vitro from axillary shoot buds. It was maintained in the form of shoot cultures on MS medium supplemented with 1.0 mg l(-1) 6-benzyladenine (BA) and 0.1 mg l(-1) alpha-naphthaleneacetic acid and excised root cultures were maintained on 1/2MS medium with 0.5 mg l(-1) indole-3-butyric acid (IBA). Shoot cultures, adventitious roots and excised root cultures were analysed by HPLC techniques for the presence of secoiridoids and xanthones. Gentiopicrin and swertiamarin, the dominant components of shoot cultures, could not be detected in root cultures. Xanthones were present in both shoot and root cultures with norswertianin-1-O-primeveroside as the dominant metabolite. The secoiridoid and xanthone content, although characteristic for certain plant organs, was dependent on the concentration of plant growth regulators (BA and IBA) added to the medium. BA in the shoot multiplication stage strongly increased the secondary metabolite (SEM) content of shoot cultures. IBA had little effect on SEM accumulation in shoots during rooting, while it moderately stimulated SEM accumulation in excised root cultures.
T2  - Acta Physiologiae Plantarum
T1  - Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro
IS  - 2
VL  - 35
SP  - 3
EP  - 574
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1042
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Janković, Teodora and Zdravković-Korać, Snežana and Vinterhalter, Dragan V",
year = "2013",
abstract = "Gentiana dinarica Beck, native to the Balkan Dinaric Mountains, was established in vitro from axillary shoot buds. It was maintained in the form of shoot cultures on MS medium supplemented with 1.0 mg l(-1) 6-benzyladenine (BA) and 0.1 mg l(-1) alpha-naphthaleneacetic acid and excised root cultures were maintained on 1/2MS medium with 0.5 mg l(-1) indole-3-butyric acid (IBA). Shoot cultures, adventitious roots and excised root cultures were analysed by HPLC techniques for the presence of secoiridoids and xanthones. Gentiopicrin and swertiamarin, the dominant components of shoot cultures, could not be detected in root cultures. Xanthones were present in both shoot and root cultures with norswertianin-1-O-primeveroside as the dominant metabolite. The secoiridoid and xanthone content, although characteristic for certain plant organs, was dependent on the concentration of plant growth regulators (BA and IBA) added to the medium. BA in the shoot multiplication stage strongly increased the secondary metabolite (SEM) content of shoot cultures. IBA had little effect on SEM accumulation in shoots during rooting, while it moderately stimulated SEM accumulation in excised root cultures.",
journal = "Acta Physiologiae Plantarum",
title = "Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro",
number = "2",
volume = "35",
pages = "3-574",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1042"
}

Vinterhalter, B., Krstić Milošević, D., Janković, T., Zdravković-Korać, S.,& Vinterhalter, D. V.. (2013). Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro. in Acta Physiologiae Plantarum, 35(2), 3-574.
https://hdl.handle.net/21.15107/rcub_ibiss_1042

Vinterhalter B, Krstić Milošević D, Janković T, Zdravković-Korać S, Vinterhalter DV. Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro. in Acta Physiologiae Plantarum. 2013;35(2):3-574.
https://hdl.handle.net/21.15107/rcub_ibiss_1042 .

Vinterhalter, Branka, Krstić Milošević, Dijana, Janković, Teodora, Zdravković-Korać, Snežana, Vinterhalter, Dragan V, "Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro" in Acta Physiologiae Plantarum, 35, no. 2 (2013):3-574,
https://hdl.handle.net/21.15107/rcub_ibiss_1042 .

TY  - JOUR
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Vinterhalter, Branka
AU  - Menković, Nebojsa R
AU  - Aljancić, Ivana S
AU  - Vinterhalter, Dragan V
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/955
AB  - The effects of different types and concentrations of sugars on root growth and xanthone production in root culture of Gentiana dinarica were investigated. The results showed that sucrose, glucose and fructose all supported root growth, and sucrose was superior in terms of growth index, dry mass and fresh/dry mass ratio then fructose or glucose at the same concentrations. However, considering equimolar concentration of sugars, their contribution to the root growth was similar. The HPLC analysis of roots indicated the presence of xanthone compounds, and the contents of norswertianin-1-O-primeveroside (1), norswertianin-1-O-glucoside (2), gentioside (3) and norswertianin (4) were evaluated. In all samples, norswertianin-1-O-primeveroside (1) was present in highest concentration, followed by norswertianin-1-O-glucoside (2), whereas gentioside (3) and norswertianin (4) were present in lower amounts. The production of xanthones was affected by both type and concentration of sugar. In general, roots growing in media supplemented with sucrose contained higher levels of xanthones. The amounts of xanthone primeveroses (1) and (3) increased with the increase of concentrations of all types of sugars, whereas higher sugar concentrations resulted in reduction of the contents of norswertianin-1-O-glucoside (2) and aglycone norswertianin (4). The roots were also evaluated regarding the content of total phenolics and higher accumulation of total phenolic compounds was observed in roots grown in fructose-containing medium. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and high correlation between total phenolic content and antiradical activity was observed (r = -0.83).
T2  - Plant Growth Regulation
T1  - Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck
IS  - 2
VL  - 71
SP  - 15
EP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_955
ER  - 

@article{
author = "Krstić Milošević, Dijana and Janković, Teodora and Vinterhalter, Branka and Menković, Nebojsa R and Aljancić, Ivana S and Vinterhalter, Dragan V",
year = "2013",
abstract = "The effects of different types and concentrations of sugars on root growth and xanthone production in root culture of Gentiana dinarica were investigated. The results showed that sucrose, glucose and fructose all supported root growth, and sucrose was superior in terms of growth index, dry mass and fresh/dry mass ratio then fructose or glucose at the same concentrations. However, considering equimolar concentration of sugars, their contribution to the root growth was similar. The HPLC analysis of roots indicated the presence of xanthone compounds, and the contents of norswertianin-1-O-primeveroside (1), norswertianin-1-O-glucoside (2), gentioside (3) and norswertianin (4) were evaluated. In all samples, norswertianin-1-O-primeveroside (1) was present in highest concentration, followed by norswertianin-1-O-glucoside (2), whereas gentioside (3) and norswertianin (4) were present in lower amounts. The production of xanthones was affected by both type and concentration of sugar. In general, roots growing in media supplemented with sucrose contained higher levels of xanthones. The amounts of xanthone primeveroses (1) and (3) increased with the increase of concentrations of all types of sugars, whereas higher sugar concentrations resulted in reduction of the contents of norswertianin-1-O-glucoside (2) and aglycone norswertianin (4). The roots were also evaluated regarding the content of total phenolics and higher accumulation of total phenolic compounds was observed in roots grown in fructose-containing medium. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and high correlation between total phenolic content and antiradical activity was observed (r = -0.83).",
journal = "Plant Growth Regulation",
title = "Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck",
number = "2",
volume = "71",
pages = "15-155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_955"
}

Krstić Milošević, D., Janković, T., Vinterhalter, B., Menković, N. R., Aljancić, I. S.,& Vinterhalter, D. V.. (2013). Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck. in Plant Growth Regulation, 71(2), 15-155.
https://hdl.handle.net/21.15107/rcub_ibiss_955

Krstić Milošević D, Janković T, Vinterhalter B, Menković NR, Aljancić IS, Vinterhalter DV. Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck. in Plant Growth Regulation. 2013;71(2):15-155.
https://hdl.handle.net/21.15107/rcub_ibiss_955 .

Krstić Milošević, Dijana, Janković, Teodora, Vinterhalter, Branka, Menković, Nebojsa R, Aljancić, Ivana S, Vinterhalter, Dragan V, "Influence of carbohydrate source on xanthone content in root cultures of Gentiana dinarica Beck" in Plant Growth Regulation, 71, no. 2 (2013):15-155,
https://hdl.handle.net/21.15107/rcub_ibiss_955 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1007
AB  - A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.
T2  - In Vitro Cellular & Developmental Biology-Plant
T1  - In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)
IS  - 3
VL  - 49
SP  - 294
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1007
ER  - 

@article{
author = "Ćosić, Tatjana and Vinterhalter, Branka and Vinterhalter, Dragan V and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena M. and Bohanec, Borut and Ninković, Slavica",
year = "2013",
abstract = "A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.",
journal = "In Vitro Cellular & Developmental Biology-Plant",
title = "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)",
number = "3",
volume = "49",
pages = "294-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1007"
}

Ćosić, T., Vinterhalter, B., Vinterhalter, D. V., Banjac, N., Cingel, A., Savić, J. M., Bohanec, B.,& Ninković, S.. (2013). In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant, 49(3), 294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007

Ćosić T, Vinterhalter B, Vinterhalter DV, Banjac N, Cingel A, Savić JM, Bohanec B, Ninković S. In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant. 2013;49(3):294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .

Ćosić, Tatjana, Vinterhalter, Branka, Vinterhalter, Dragan V, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena M., Bohanec, Borut, Ninković, Slavica, "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)" in In Vitro Cellular & Developmental Biology-Plant, 49, no. 3 (2013):294-303,
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .

TY  - JOUR
AU  - Pavlović, Suzana
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Dragan V
AU  - Zdravković, Jasmina
AU  - Cvikić, Dejan
AU  - Banjac, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1005
AB  - A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.
T2  - Plant Cell Tissue and Organ Culture
T1  - Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)
IS  - 3
VL  - 113
SP  - 31
EP  - 406
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1005
ER  - 

@article{
author = "Pavlović, Suzana and Vinterhalter, Branka and Zdravković-Korać, Snežana and Vinterhalter, Dragan V and Zdravković, Jasmina and Cvikić, Dejan and Banjac, Nevena",
year = "2013",
abstract = "A simple and rapid protocol was established for repetitive somatic embryogenesis and subsequent plant regeneration in two important Brassica oleracea varieties, cabbage and cauliflower. Direct regeneration of somatic embryos (SEs) was achieved from immature zygotic embryos cultured on B5 plant growth regulator (PGR)-free (B5-0) induction medium and on B5 medium supplemented with 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) (B5-D). Zygotic embryos of both cabbage and cauliflower at the cotyledonary (C) stage (1.8 mm long) incubated on B5-0 medium displayed the highest embryo-forming capacities (EFCs) of 11.84 and 11.95, respectively. Secondary somatic embryos (SSEs) appeared on the cabbage and cauliflower's primary embryos at a high frequency (83.3 and 87.5 %, respectively), and this process continued in a repetitive way on PGR-free Murashige and Skoog (MS-0) medium. The embryogenic potential of the cultures with a gradual diminution was maintained for 10 months (ten cycles). A total of 20 % of the mature SSEs from cabbage and 55 % from cauliflower spontaneously regenerated plantlets on MS-0 medium. The addition of 1 mg l(-1) 6-benzyladenine (BA) or 6-furfurylaminopurine (Kin) in the regeneration medium significantly improved somatic embryo conversion into plantlets by up to 56 % in cabbage and 79 % in cauliflower. Regenerated plants acclimated successfully to ex vitro conditions and displayed morphological and reproductive characteristics similar to seed-derived plants. Effective recurrent somatic embryogenesis may be an appropriate practical solution for clonal propagation and genetic modifications of cabbage and cauliflower.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)",
number = "3",
volume = "113",
pages = "31-406",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1005"
}

Pavlović, S., Vinterhalter, B., Zdravković-Korać, S., Vinterhalter, D. V., Zdravković, J., Cvikić, D.,& Banjac, N.. (2013). Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture, 113(3), 31-406.
https://hdl.handle.net/21.15107/rcub_ibiss_1005

Pavlović S, Vinterhalter B, Zdravković-Korać S, Vinterhalter DV, Zdravković J, Cvikić D, Banjac N. Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis). in Plant Cell Tissue and Organ Culture. 2013;113(3):31-406.
https://hdl.handle.net/21.15107/rcub_ibiss_1005 .

Pavlović, Suzana, Vinterhalter, Branka, Zdravković-Korać, Snežana, Vinterhalter, Dragan V, Zdravković, Jasmina, Cvikić, Dejan, Banjac, Nevena, "Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)" in Plant Cell Tissue and Organ Culture, 113, no. 3 (2013):31-406,
https://hdl.handle.net/21.15107/rcub_ibiss_1005 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Janković, Teodora
AU  - Milojević, Jelena
AU  - Vinterhalter, Dragan V
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1158
AB  - Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l(-1) BA and 0.1 mg l(-1) NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5-1.0 mg l(-1) IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.
T2  - Central European Journal of Biology
T1  - In vitro propagation of Gentiana dinarica Beck.
IS  - 4
VL  - 7
EP  - 697
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1158
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Janković, Teodora and Milojević, Jelena and Vinterhalter, Dragan V",
year = "2012",
abstract = "Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l(-1) BA and 0.1 mg l(-1) NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5-1.0 mg l(-1) IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.",
journal = "Central European Journal of Biology",
title = "In vitro propagation of Gentiana dinarica Beck.",
number = "4",
volume = "7",
pages = "697",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1158"
}

Vinterhalter, B., Krstić Milošević, D., Janković, T., Milojević, J.,& Vinterhalter, D. V.. (2012). In vitro propagation of Gentiana dinarica Beck.. in Central European Journal of Biology, 7(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1158

Vinterhalter B, Krstić Milošević D, Janković T, Milojević J, Vinterhalter DV. In vitro propagation of Gentiana dinarica Beck.. in Central European Journal of Biology. 2012;7(4):null-697.
https://hdl.handle.net/21.15107/rcub_ibiss_1158 .

Vinterhalter, Branka, Krstić Milošević, Dijana, Janković, Teodora, Milojević, Jelena, Vinterhalter, Dragan V, "In vitro propagation of Gentiana dinarica Beck." in Central European Journal of Biology, 7, no. 4 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1158 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S
AU  - Savić, Jelena M.
AU  - Jovanović, Zivko S
AU  - Miljuš-Đukić, Jovanka D.
AU  - Radović, Svetlana R
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1142
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
IS  - 5
VL  - 34
SP  - 339
EP  - 2006
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1142
ER  - 

@article{
author = "Stanisavljević, Nemanja S and Savić, Jelena M. and Jovanović, Zivko S and Miljuš-Đukić, Jovanka D. and Radović, Svetlana R and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
number = "5",
volume = "34",
pages = "339-2006",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1142"
}

Stanisavljević, N. S., Savić, J. M., Jovanović, Z. S., Miljuš-Đukić, J. D., Radović, S. R., Vinterhalter, D. V.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum, 34(5), 339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142

Stanisavljević NS, Savić JM, Jovanović ZS, Miljuš-Đukić JD, Radović SR, Vinterhalter DV, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

Stanisavljević, Nemanja S, Savić, Jelena M., Jovanović, Zivko S, Miljuš-Đukić, Jovanka D., Radović, Svetlana R, Vinterhalter, Dragan V, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):339-2006,
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

TY  - JOUR
AU  - Janković, Teodora
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Nikolić, Radomirka R
AU  - Vinterhalter, Dragan V
AU  - Milosavljević, Slobodan M
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1282
AB  - Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).
T2  - Acta Physiologiae Plantarum
T1  - Xanthone compounds in shoot cultures of Gentianella bulgarica
IS  - 4
VL  - 33
EP  - 1520
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1282
ER  - 

@article{
author = "Janković, Teodora and Vinterhalter, Branka and Krstić Milošević, Dijana and Nikolić, Radomirka R and Vinterhalter, Dragan V and Milosavljević, Slobodan M",
year = "2011",
abstract = "Shoot cultures of Gentianella bulgarica established from seedling epicotyls were maintained on MS medium supplemented with BA 0.2 mg l(-1) + NAA 0.1 mg l(-1). Cultures were prone to precocious flowering requiring the use of small shoot buds for multiplication purposes. The contents of three xanthone compounds identified as DGL, BGL, and DMB, in different plant material were determined by HPLC. The analysis revealed that the production of xanthones was affected by different concentrations of BA in medium. Shoot cultures grown at higher BA concentrations contained more DGL than material grown in nature. The concentrations of other two xanthones were lower in shoot cultures than in plants from nature. The radical scavenging activity of plant extracts and xanthones was investigated by DPPH test. Samples from plants grown in nature showed the highest activity (IC(50) = 0.26 mg ml(-1)), while the extracts of shoot cultures grown in media with higher concentrations of BA showed moderate activities (IC(50) from 1.6 to 4.4 mg ml(-1)).",
journal = "Acta Physiologiae Plantarum",
title = "Xanthone compounds in shoot cultures of Gentianella bulgarica",
number = "4",
volume = "33",
pages = "1520",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1282"
}

Janković, T., Vinterhalter, B., Krstić Milošević, D., Nikolić, R. R., Vinterhalter, D. V.,& Milosavljević, S. M.. (2011). Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum, 33(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1282

Janković T, Vinterhalter B, Krstić Milošević D, Nikolić RR, Vinterhalter DV, Milosavljević SM. Xanthone compounds in shoot cultures of Gentianella bulgarica. in Acta Physiologiae Plantarum. 2011;33(4):null-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

Janković, Teodora, Vinterhalter, Branka, Krstić Milošević, Dijana, Nikolić, Radomirka R, Vinterhalter, Dragan V, Milosavljević, Slobodan M, "Xanthone compounds in shoot cultures of Gentianella bulgarica" in Acta Physiologiae Plantarum, 33, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1282 .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Ćalić-Dragosavac, Dušica D.
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1360
AB  - An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.
T2  - African Journal of Biotechnology
T1  - Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)
IS  - 30
VL  - 9
EP  - 4650
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1360
ER  - 

@article{
author = "Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan V and Ćalić-Dragosavac, Dušica D. and Smigocki, Ann and Ninković, Slavica",
year = "2010",
abstract = "An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.",
journal = "African Journal of Biotechnology",
title = "Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)",
number = "30",
volume = "9",
pages = "4650",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1360"
}

Cingel, A., Vinterhalter, B., Vinterhalter, D. V., Ćalić-Dragosavac, D. D., Smigocki, A.,& Ninković, S.. (2010). Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica). in African Journal of Biotechnology, 9(30).
https://hdl.handle.net/21.15107/rcub_ibiss_1360

Cingel A, Vinterhalter B, Vinterhalter DV, Ćalić-Dragosavac DD, Smigocki A, Ninković S. Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica). in African Journal of Biotechnology. 2010;9(30):null-4650.
https://hdl.handle.net/21.15107/rcub_ibiss_1360 .

Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan V, Ćalić-Dragosavac, Dušica D., Smigocki, Ann, Ninković, Slavica, "Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)" in African Journal of Biotechnology, 9, no. 30 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1360 .

TY  - CONF
AU  - Đorđević, Tatjana
AU  - Dragicević, Ivana C
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1396
C3  - In Vitro Cellular & Developmental Biology-Animal
T1  - Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration
IS  - null
VL  - 46
EP  - S109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1396
ER  - 

@conference{
author = "Đorđević, Tatjana and Dragicević, Ivana C and Vinterhalter, Branka and Vinterhalter, Dragan V and Motyka, Vaclav and Ninković, Slavica",
year = "2010",
journal = "In Vitro Cellular & Developmental Biology-Animal",
title = "Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration",
number = "null",
volume = "46",
pages = "S109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1396"
}

Đorđević, T., Dragicević, I. C., Vinterhalter, B., Vinterhalter, D. V., Motyka, V.,& Ninković, S.. (2010). Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration. in In Vitro Cellular & Developmental Biology-Animal, 46(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1396

Đorđević T, Dragicević IC, Vinterhalter B, Vinterhalter DV, Motyka V, Ninković S. Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration. in In Vitro Cellular & Developmental Biology-Animal. 2010;46(null):null-S109.
https://hdl.handle.net/21.15107/rcub_ibiss_1396 .

Đorđević, Tatjana, Dragicević, Ivana C, Vinterhalter, Branka, Vinterhalter, Dragan V, Motyka, Vaclav, Ninković, Slavica, "Effects of Explant Types and Different Cytokinins on Kohlrabi Regeneration" in In Vitro Cellular & Developmental Biology-Animal, 46, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1396 .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Dodig, Dejan
AU  - Nikolić, Radomirka R
AU  - Ninković, Slavica
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1445
AB  - The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.
T2  - Russian Journal of Plant Physiology
T1  - Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
IS  - 4
VL  - 56
EP  - 545
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1445
ER  - 

@article{
author = "Banjac, Nevena and Dodig, Dejan and Nikolić, Radomirka R and Ninković, Slavica and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2009",
abstract = "The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12-15 days after anthesis from field-grown plants, were cultured on Murashige and Skoog medium containing 2 mg/l 2,4-D followed by their transfer to a growth regulator-free medium. Donor plants growth in a season 2003 characterized by drought and heat stress, as compared to favorable growth seasons 2004 and 2005, resulted in a increased variability and a decreased percentage of callus formation and the number of regenerating calli and plants regenerated per embryo in all genotypes, except genotype Florida that exhibited a significantly increased number of regenerating calli in 2003. The reduction rate of regenerating callus formation depended on genotype, and it was the highest in Magnif 41, NS66/92, and Mexico 3. The results suggest that the impact of donor plant growth conditions on TCR traits can be as large as the effect of the genotype, which should be taken into account when planning transformation work with wheat.",
journal = "Russian Journal of Plant Physiology",
title = "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes",
number = "4",
volume = "56",
pages = "545",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1445"
}

Banjac, N., Dodig, D., Nikolić, R. R., Ninković, S., Vinterhalter, D. V.,& Vinterhalter, B.. (2009). Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology, 56(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1445

Banjac N, Dodig D, Nikolić RR, Ninković S, Vinterhalter DV, Vinterhalter B. Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes. in Russian Journal of Plant Physiology. 2009;56(4):null-545.
https://hdl.handle.net/21.15107/rcub_ibiss_1445 .

Banjac, Nevena, Dodig, Dejan, Nikolić, Radomirka R, Ninković, Slavica, Vinterhalter, Dragan V, Vinterhalter, Branka, "Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes" in Russian Journal of Plant Physiology, 56, no. 4 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1445 .

TY  - CONF
AU  - Vinterhalter, Branka S.
AU  - Savić, Jelena M.
AU  - Platiša, Jelena
AU  - Raspor, Martin
AU  - Ninković, Slavica B.
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan V
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1518
AB  - Shoot cultures of nickel hyperaccumulating Alyssum murale were established from epicotyl explants of seedlings aseptically germinated on hormone-free MS medium. They were further maintained on media with 0-0.92 mu M kinetin. Optimal shoot multiplication was at 0.46 mu M kinetin. Inoculation by shoot wounding was performed with overnight suspension of A. rhizogenes A4M70GUS which contains GUS gene cointegrated in pRiA4. After 30 days hairy roots were produced at the wounding site in 31 explant (25% out of 124). Hairy roots were excised and further propagated on hormone-free medium as separate clones. In the first passage clones 3 and 6 could be distinguished by fast growth and spontaneous shoot regeneration. In other clones (12, 23 and 25) shoot regeneration required presence of cytokinins. The five shoot culture clones regenerated from hairy roots were further cultured on media with 0.46 mu M kinetin. These shoots were characterized by good elongation and lateral shoot branching, short internodes, minute slightly curled leaves and well developed plagiotropic root system spreading over the surface of media. Thus all plants regenerated from hairy root cultures manifested the characteristic Ri syndrome phenotype. They all had a strong positive GUS reaction. PCR analysis confirmed presence of uidA sequence from the gus construct. They were also tolerant to nickel accumulating up to 24,700 mu g g(-1) dry weight.
C3  - Plant Cell Tissue and Organ Culture
T1  - Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit
IS  - 3
VL  - 94
SP  - 299
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1518
ER  - 

@conference{
author = "Vinterhalter, Branka S. and Savić, Jelena M. and Platiša, Jelena and Raspor, Martin and Ninković, Slavica B. and Banjac, Nevena and Vinterhalter, Dragan V",
year = "2008",
abstract = "Shoot cultures of nickel hyperaccumulating Alyssum murale were established from epicotyl explants of seedlings aseptically germinated on hormone-free MS medium. They were further maintained on media with 0-0.92 mu M kinetin. Optimal shoot multiplication was at 0.46 mu M kinetin. Inoculation by shoot wounding was performed with overnight suspension of A. rhizogenes A4M70GUS which contains GUS gene cointegrated in pRiA4. After 30 days hairy roots were produced at the wounding site in 31 explant (25% out of 124). Hairy roots were excised and further propagated on hormone-free medium as separate clones. In the first passage clones 3 and 6 could be distinguished by fast growth and spontaneous shoot regeneration. In other clones (12, 23 and 25) shoot regeneration required presence of cytokinins. The five shoot culture clones regenerated from hairy roots were further cultured on media with 0.46 mu M kinetin. These shoots were characterized by good elongation and lateral shoot branching, short internodes, minute slightly curled leaves and well developed plagiotropic root system spreading over the surface of media. Thus all plants regenerated from hairy root cultures manifested the characteristic Ri syndrome phenotype. They all had a strong positive GUS reaction. PCR analysis confirmed presence of uidA sequence from the gus construct. They were also tolerant to nickel accumulating up to 24,700 mu g g(-1) dry weight.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit",
number = "3",
volume = "94",
pages = "299-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1518"
}

Vinterhalter, B. S., Savić, J. M., Platiša, J., Raspor, M., Ninković, S. B., Banjac, N.,& Vinterhalter, D. V.. (2008). Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit. in Plant Cell Tissue and Organ Culture, 94(3), 299-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1518

Vinterhalter BS, Savić JM, Platiša J, Raspor M, Ninković SB, Banjac N, Vinterhalter DV. Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit. in Plant Cell Tissue and Organ Culture. 2008;94(3):299-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1518 .

Vinterhalter, Branka S., Savić, Jelena M., Platiša, Jelena, Raspor, Martin, Ninković, Slavica B., Banjac, Nevena, Vinterhalter, Dragan V, "Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit" in Plant Cell Tissue and Organ Culture, 94, no. 3 (2008):299-303,
https://hdl.handle.net/21.15107/rcub_ibiss_1518 .

TY  - JOUR
AU  - Dragicević, Ivana C
AU  - Konjević, Radomir M
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Nešković, Mirjana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1540
AB  - Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.
T2  - Plant Growth Regulation
T1  - The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro
IS  - 3
VL  - 54
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1540
ER  - 

@article{
author = "Dragicević, Ivana C and Konjević, Radomir M and Vinterhalter, Branka and Vinterhalter, Dragan V and Nešković, Mirjana",
year = "2008",
abstract = "Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.",
journal = "Plant Growth Regulation",
title = "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro",
number = "3",
volume = "54",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1540"
}

Dragicević, I. C., Konjević, R. M., Vinterhalter, B., Vinterhalter, D. V.,& Nešković, M.. (2008). The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation, 54(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1540

Dragicević IC, Konjević RM, Vinterhalter B, Vinterhalter DV, Nešković M. The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation. 2008;54(3):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

Dragicević, Ivana C, Konjević, Radomir M, Vinterhalter, Branka, Vinterhalter, Dragan V, Nešković, Mirjana, "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro" in Plant Growth Regulation, 54, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana D.
AU  - Ninković, Slavica
AU  - Uzelac, Branka
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1583
AB  - Two inbred lines of Brassica oleracea L. var. capitata were transformed with two Agrobacterium tumefaciens strains harboring resistance to herbicide Basta: AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Inoculated cotyledons and hypocotyls provided equally good explants and manifested a high percentage of shoot regeneration on MS medium supplemented with 1 mg/l benzyladenine and 0.5 mg/l indole-3-butyric acid. The P34I5 genotype was superior to P22I5 in shoot regeneration (48.1 vs. 26.9%), multiplication, and acclimation in the greenhouse (76 vs. 40%). A. tumefaciens AGL1/pDM805 provided more regenerated shoots per explant, especially in the case of cotyledon explants, and the higher transformation rate (up to 35% vs. up to 12%) as compared to LB5-1. Putative transformants survived spraying with 10-30 mg/l phosphinothricin. Transformation was confirmed by GUS assay and PCR analysis in T0 and T1 generations.
T2  - Russian Journal of Plant Physiology
T1  - Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata
IS  - 5
VL  - 54
EP  - 658
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1583
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana D. and Ninković, Slavica and Uzelac, Branka and Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2007",
abstract = "Two inbred lines of Brassica oleracea L. var. capitata were transformed with two Agrobacterium tumefaciens strains harboring resistance to herbicide Basta: AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Inoculated cotyledons and hypocotyls provided equally good explants and manifested a high percentage of shoot regeneration on MS medium supplemented with 1 mg/l benzyladenine and 0.5 mg/l indole-3-butyric acid. The P34I5 genotype was superior to P22I5 in shoot regeneration (48.1 vs. 26.9%), multiplication, and acclimation in the greenhouse (76 vs. 40%). A. tumefaciens AGL1/pDM805 provided more regenerated shoots per explant, especially in the case of cotyledon explants, and the higher transformation rate (up to 35% vs. up to 12%) as compared to LB5-1. Putative transformants survived spraying with 10-30 mg/l phosphinothricin. Transformation was confirmed by GUS assay and PCR analysis in T0 and T1 generations.",
journal = "Russian Journal of Plant Physiology",
title = "Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata",
number = "5",
volume = "54",
pages = "658",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1583"
}

Sretenović-Rajičić, T. D., Ninković, S., Uzelac, B., Vinterhalter, B.,& Vinterhalter, D. V.. (2007). Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata. in Russian Journal of Plant Physiology, 54(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1583

Sretenović-Rajičić TD, Ninković S, Uzelac B, Vinterhalter B, Vinterhalter DV. Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata. in Russian Journal of Plant Physiology. 2007;54(5):null-658.
https://hdl.handle.net/21.15107/rcub_ibiss_1583 .

Sretenović-Rajičić, Tatjana D., Ninković, Slavica, Uzelac, Branka, Vinterhalter, Branka, Vinterhalter, Dragan V, "Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata" in Russian Journal of Plant Physiology, 54, no. 5 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1583 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Dragan V
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1625
AB  - Hairy root cultures of Hypericum perforatum were obtained following inoculation of aseptically germinated seedlings with A. rhizogenes strain A4M70GUS. Effect of sucrose on the growth and biomass production of hairy root cultures was investigated. Hairy root cultures spontaneously regenerated shoots buds from which a number of shoot culture clones was established. Transformed shoot cultures exhibited good shoot multiplication, elongation and rooting on a hormone-free woody plant medium. Plants regenerated from hairy roots were similar in appearance to the normal, nontransformed plants.
T2  - Biologia Plantarum
T1  - Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS
IS  - 4
VL  - 50
EP  - 770
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1625
ER  - 

@article{
author = "Vinterhalter, Branka and Ninković, Slavica and Cingel, Aleksandar and Vinterhalter, Dragan V",
year = "2006",
abstract = "Hairy root cultures of Hypericum perforatum were obtained following inoculation of aseptically germinated seedlings with A. rhizogenes strain A4M70GUS. Effect of sucrose on the growth and biomass production of hairy root cultures was investigated. Hairy root cultures spontaneously regenerated shoots buds from which a number of shoot culture clones was established. Transformed shoot cultures exhibited good shoot multiplication, elongation and rooting on a hormone-free woody plant medium. Plants regenerated from hairy roots were similar in appearance to the normal, nontransformed plants.",
journal = "Biologia Plantarum",
title = "Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS",
number = "4",
volume = "50",
pages = "770",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1625"
}

Vinterhalter, B., Ninković, S., Cingel, A.,& Vinterhalter, D. V.. (2006). Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1625

Vinterhalter B, Ninković S, Cingel A, Vinterhalter DV. Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS. in Biologia Plantarum. 2006;50(4):null-770.
https://hdl.handle.net/21.15107/rcub_ibiss_1625 .

Vinterhalter, Branka, Ninković, Slavica, Cingel, Aleksandar, Vinterhalter, Dragan V, "Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1625 .

TY  - JOUR
AU  - Sretenović-Rajičić, Tatjana D.
AU  - Ninković, Slavica
AU  - Miljuš-Đukić, Jovanka D.
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1624
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.
T2  - Biologia Plantarum
T1  - Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata
IS  - 4
VL  - 50
EP  - 530
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1624
ER  - 

@article{
author = "Sretenović-Rajičić, Tatjana D. and Ninković, Slavica and Miljuš-Đukić, Jovanka D. and Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2006",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained using hypocotyl and cotyledon explants. The percentage of explants which formed roots was very high in all genotypes: 92.3 % in Savoy Gg-1, 64.4 % in cabbage P22I5, and 87.2 % in P34I5. Spontaneous shoot regeneration of excised root cultures grown on the hormone-free medium occurred in all three genotypes. In cabbage lines P22I5 and P34I5 shoot regeneration was higher (9.3 and 2.6 % respectively) than in Savoy cabbage Gg-1 (1.3 %). Transgenic nature of hairy root-derived plants was evaluated by GUS histological test and PCR analysis. All the tested cabbage shoots were GUS positive whilst in a Savoy cabbage GUS expression was registered only in 55 % of tested clones. PCR analysis demonstrated the presence of the GUS gene in regenerated shoot clones and in T-1 progeny.",
journal = "Biologia Plantarum",
title = "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata",
number = "4",
volume = "50",
pages = "530",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1624"
}

Sretenović-Rajičić, T. D., Ninković, S., Miljuš-Đukić, J. D., Vinterhalter, B.,& Vinterhalter, D. V.. (2006). Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1624

Sretenović-Rajičić TD, Ninković S, Miljuš-Đukić JD, Vinterhalter B, Vinterhalter DV. Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata. in Biologia Plantarum. 2006;50(4):null-530.
https://hdl.handle.net/21.15107/rcub_ibiss_1624 .

Sretenović-Rajičić, Tatjana D., Ninković, Slavica, Miljuš-Đukić, Jovanka D., Vinterhalter, Branka, Vinterhalter, Dragan V, "Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B-oleracea var. capitata" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1624 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1705
AB  - Shoot cultures of Alyssum markgrafii O.E. Shulz, endemic nickel hyperaccumulating species of central Balkan, were established and maintained on Murashige and Skoog medium supplemented with 0.2 mg dm(-3) benzyladenine (BA). Nickel in form of NiCl2 . 6 H2O was supplemented at 22 different concentrations ranging from 0.0001 to 15 mM but none of them was lethal to cultures. High Ni2+ concentrations (10 mM or more) arrested shoot growth which, upon transfer to Ni-free medium, commenced via axillary bud proliferation. Shoots that developed from axillary buds through the subculture manifested increased tolerance to Ni2+ expressed as shoot elongation. Shoot multiplication and dry biomass production decreased with increase of N2+ in medium. Only the accumulation of Ni2+ in tissues increased with Ni2+ content of the medium. Apart from shoot cultures, high Ni2+ accumulation was registered in undifferentiated callus cultured on medium with 0.5 mg dm(-3) BA and 0.5 mg dm(-3) naphthylacetic acid. Highest content of accumulated Ni was 2.37 mu g g(-1) (d.m.) in shoots and 2.65 mu g g(-1) (d.m.) in callus, both measured on medium with 15 mM Ni2+.
T2  - Biologia Plantarum
T1  - Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii
IS  - 1
VL  - 49
EP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1705
ER  - 

@article{
author = "Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2005",
abstract = "Shoot cultures of Alyssum markgrafii O.E. Shulz, endemic nickel hyperaccumulating species of central Balkan, were established and maintained on Murashige and Skoog medium supplemented with 0.2 mg dm(-3) benzyladenine (BA). Nickel in form of NiCl2 . 6 H2O was supplemented at 22 different concentrations ranging from 0.0001 to 15 mM but none of them was lethal to cultures. High Ni2+ concentrations (10 mM or more) arrested shoot growth which, upon transfer to Ni-free medium, commenced via axillary bud proliferation. Shoots that developed from axillary buds through the subculture manifested increased tolerance to Ni2+ expressed as shoot elongation. Shoot multiplication and dry biomass production decreased with increase of N2+ in medium. Only the accumulation of Ni2+ in tissues increased with Ni2+ content of the medium. Apart from shoot cultures, high Ni2+ accumulation was registered in undifferentiated callus cultured on medium with 0.5 mg dm(-3) BA and 0.5 mg dm(-3) naphthylacetic acid. Highest content of accumulated Ni was 2.37 mu g g(-1) (d.m.) in shoots and 2.65 mu g g(-1) (d.m.) in callus, both measured on medium with 15 mM Ni2+.",
journal = "Biologia Plantarum",
title = "Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii",
number = "1",
volume = "49",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1705"
}

Vinterhalter, B.,& Vinterhalter, D. V.. (2005). Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii. in Biologia Plantarum, 49(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1705

Vinterhalter B, Vinterhalter DV. Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii. in Biologia Plantarum. 2005;49(1):null-124.
https://hdl.handle.net/21.15107/rcub_ibiss_1705 .

Vinterhalter, Branka, Vinterhalter, Dragan V, "Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii" in Biologia Plantarum, 49, no. 1 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1705 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1799
AB  - Direct somatic embryogenesis in celandine (Chelidonium majus L.) was achieved in epicotyl explants of seedlings after prolonged cultivation on Murashige and Skoog medium with or without plant growth regulators. Somatic embryos developed into plantlets which entered additional cycles of somatic embryogenesis. Cultures consisting of plantlets with prolonged embryogenic potential were maintained for five years on plant growth regulator free medium. Embryos which developed into rooted plantlets could be acclimated in a glasshouse enabling thus a continuous propagation scheme to be established.
T2  - Biologia Plantarum
T1  - Propagation of Chelidonium majus L. by somatic embryogenesis
IS  - 4
VL  - 45
EP  - 493
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1799
ER  - 

@article{
author = "Vinterhalter, Branka and Vinterhalter, Dragan V",
year = "2002",
abstract = "Direct somatic embryogenesis in celandine (Chelidonium majus L.) was achieved in epicotyl explants of seedlings after prolonged cultivation on Murashige and Skoog medium with or without plant growth regulators. Somatic embryos developed into plantlets which entered additional cycles of somatic embryogenesis. Cultures consisting of plantlets with prolonged embryogenic potential were maintained for five years on plant growth regulator free medium. Embryos which developed into rooted plantlets could be acclimated in a glasshouse enabling thus a continuous propagation scheme to be established.",
journal = "Biologia Plantarum",
title = "Propagation of Chelidonium majus L. by somatic embryogenesis",
number = "4",
volume = "45",
pages = "493",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1799"
}

Vinterhalter, B.,& Vinterhalter, D. V.. (2002). Propagation of Chelidonium majus L. by somatic embryogenesis. in Biologia Plantarum, 45(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1799

Vinterhalter B, Vinterhalter DV. Propagation of Chelidonium majus L. by somatic embryogenesis. in Biologia Plantarum. 2002;45(4):null-493.
https://hdl.handle.net/21.15107/rcub_ibiss_1799 .

Vinterhalter, Branka, Vinterhalter, Dragan V, "Propagation of Chelidonium majus L. by somatic embryogenesis" in Biologia Plantarum, 45, no. 4 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1799 .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Nešković, Mirjana
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1822
AB  - Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 - 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 to 74.1 mu mol m(-2) s(-1), leaf area increased 5-fold. Sucrose also significantly increased leaf area and the maxima were at concentration 147 mM at high irradiance and 233.6 mM at low irradiance. Sucrose was superior to Fructose, glucose and combination of both in increasing leaf area. Decreasing concentration of KNO3 and NH4NO3 caused a 3-fold decline of leaf area.
T2  - Biologia Plantarum
T1  - Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.
IS  - 2
VL  - 44
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1822
ER  - 

@article{
author = "Vinterhalter, Branka and Vinterhalter, Dragan V and Nešković, Mirjana",
year = "2001",
abstract = "Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 - 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 to 74.1 mu mol m(-2) s(-1), leaf area increased 5-fold. Sucrose also significantly increased leaf area and the maxima were at concentration 147 mM at high irradiance and 233.6 mM at low irradiance. Sucrose was superior to Fructose, glucose and combination of both in increasing leaf area. Decreasing concentration of KNO3 and NH4NO3 caused a 3-fold decline of leaf area.",
journal = "Biologia Plantarum",
title = "Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.",
number = "2",
volume = "44",
pages = "188",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1822"
}

Vinterhalter, B., Vinterhalter, D. V.,& Nešković, M.. (2001). Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.. in Biologia Plantarum, 44(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1822

Vinterhalter B, Vinterhalter DV, Nešković M. Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L.. in Biologia Plantarum. 2001;44(2):null-188.
https://hdl.handle.net/21.15107/rcub_ibiss_1822 .

Vinterhalter, Branka, Vinterhalter, Dragan V, Nešković, Mirjana, "Effect of irradiance, sugars and nitrogen on leaf size of in vitro grown Ceratonia siliqua L." in Biologia Plantarum, 44, no. 2 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1822 .