TY  - CHAP
AU  - Krstić Milošević, Dijana
AU  - Vinterhalter, Branka
AU  - Janković, Teodora
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2571
UR  - http://link.springer.com/chapter/10.1007%2F978-3-642-54102-5_4
AB  - In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.
PB  - Springer Berlin Heidelberg, Berlin, Heidelberg
T2  - The Gentianaceae - Volume 2: Biotechnology and Applications
T1  - Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula
DO  - 10.1007/978-3-642-54102-5
SP  - 93
EP  - 112
ER  - 

@inbook{
editor = "Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna",
author = "Krstić Milošević, Dijana and Vinterhalter, Branka and Janković, Teodora and Vinterhalter, Dragan",
year = "2015",
abstract = "In the central regions of the Balkan Peninsula, the genus Gentianella is represented by six species. Five of them G. albanica, G. austriaca, G. bulgarica, G. ciliata, and G. crispata were subject of phytochemical and in vitro studies. Two species, G. austriaca and G. bulgarica, were established as shoot cultures and procedures developed for their in vitro propagation. Difficulties with seed germination and precocious flowering affected the final propagation stages. Phytochemical analyses were performed on five species from nature and on cultured shoots. Plant material was rich in secondary metabolites with xanthones, flavone-C-glucosides, and secoiridoids as the principal constituents. The xanthone aglycones, demethy- bellidifolin, bellidifolin, corymbiferin, and their corresponding glucosides were dominant components in all the species investigated. The predominant substitution pattern of xanthones found in the genus Gentianella was 1,3,5,8 and to a lesser extent, 1,3,7,8. Lanceoside and veratriloside were new compounds found in European Gentianella species. In vitro material generally contained less secondary metabolites than plants from nature; the content of secondary products was influenced by the concentration of benzyladenine (BAP) in the culture medium.",
publisher = "Springer Berlin Heidelberg, Berlin, Heidelberg",
journal = "The Gentianaceae - Volume 2: Biotechnology and Applications",
booktitle = "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula",
doi = "10.1007/978-3-642-54102-5",
pages = "93-112"
}

Rybczyński, J. J., Davey, M. R., Mikuła, A., Krstić Milošević, D., Vinterhalter, B., Janković, T.,& Vinterhalter, D.. (2015). Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications
Springer Berlin Heidelberg, Berlin, Heidelberg., 93-112.
https://doi.org/10.1007/978-3-642-54102-5

Rybczyński JJ, Davey MR, Mikuła A, Krstić Milošević D, Vinterhalter B, Janković T, Vinterhalter D. Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula. in The Gentianaceae - Volume 2: Biotechnology and Applications. 2015;:93-112.
doi:10.1007/978-3-642-54102-5 .

Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna, Krstić Milošević, Dijana, Vinterhalter, Branka, Janković, Teodora, Vinterhalter, Dragan, "Biotechnology and Phytochemistry of Gentianella Species from the Central Regions of the Balkan Peninsula" in The Gentianaceae - Volume 2: Biotechnology and Applications (2015):93-112,
https://doi.org/10.1007/978-3-642-54102-5 . .

TY  - CHAP
AU  - Trifunović, Milana
AU  - Subotić, Angelina
AU  - Petrić, Marija
AU  - Jevremović, Slađana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2578
UR  - http://link.springer.com/chapter/10.1007%2F978-3-642-54102-5_5
AB  - Histochemical localization using the β-Glc Yariv reagent and immuno-localization with arabinogalactan protein (AGP) reactive antibodies (LM2, JIM13, JIM15, JIM16, MAC207) were performed during morphogenic induction in root cultures of Centaurium erythraea Rafn cultured on half-strength MS medium without plant growth regulators. The observations revealed that β-Glc Yariv reagent specifically bound to AGPs in cells of the root epidermis and central cylinder. Monoclonal antibodies recognizing AGPs were localized in epidermal cells and cells of the central cylinder (LM2, JIM16), vascular tissue (JIM15), globular somatic embryos (LM2, MAC207), and de novo-formed meristematic centers in the root cortex (JIM16). The effect of β-Glc Yariv reagent was investigated after supplementation (0-75 µM) in the culture medium. The morphogenetic potential was increased at lower concentrations (15-25 µM) of treatment with β-Glc Yariv reagent but inhibited (40%) at the highest concentration. These results implicate that AGPs play a significant role during the development of somatic embryos and adventitious shoots in root cultures of C. erythraea.
PB  - Springer Berlin Heidelberg, Berlin, Heidelberg
T2  - The Gentianaceae - Volume 2: Biotechnology and Applications
T1  - The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro
DO  - 10.1007/978-3-642-54102-5
SP  - 113
EP  - 138
ER  - 

@inbook{
editor = "Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna",
author = "Trifunović, Milana and Subotić, Angelina and Petrić, Marija and Jevremović, Slađana",
year = "2015",
abstract = "Histochemical localization using the β-Glc Yariv reagent and immuno-localization with arabinogalactan protein (AGP) reactive antibodies (LM2, JIM13, JIM15, JIM16, MAC207) were performed during morphogenic induction in root cultures of Centaurium erythraea Rafn cultured on half-strength MS medium without plant growth regulators. The observations revealed that β-Glc Yariv reagent specifically bound to AGPs in cells of the root epidermis and central cylinder. Monoclonal antibodies recognizing AGPs were localized in epidermal cells and cells of the central cylinder (LM2, JIM16), vascular tissue (JIM15), globular somatic embryos (LM2, MAC207), and de novo-formed meristematic centers in the root cortex (JIM16). The effect of β-Glc Yariv reagent was investigated after supplementation (0-75 µM) in the culture medium. The morphogenetic potential was increased at lower concentrations (15-25 µM) of treatment with β-Glc Yariv reagent but inhibited (40%) at the highest concentration. These results implicate that AGPs play a significant role during the development of somatic embryos and adventitious shoots in root cultures of C. erythraea.",
publisher = "Springer Berlin Heidelberg, Berlin, Heidelberg",
journal = "The Gentianaceae - Volume 2: Biotechnology and Applications",
booktitle = "The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro",
doi = "10.1007/978-3-642-54102-5",
pages = "113-138"
}

Rybczyński, J. J., Davey, M. R., Mikuła, A., Trifunović, M., Subotić, A., Petrić, M.,& Jevremović, S.. (2015). The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro. in The Gentianaceae - Volume 2: Biotechnology and Applications
Springer Berlin Heidelberg, Berlin, Heidelberg., 113-138.
https://doi.org/10.1007/978-3-642-54102-5

Rybczyński JJ, Davey MR, Mikuła A, Trifunović M, Subotić A, Petrić M, Jevremović S. The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro. in The Gentianaceae - Volume 2: Biotechnology and Applications. 2015;:113-138.
doi:10.1007/978-3-642-54102-5 .

Rybczyński, Jan J., Davey, Michael R., Mikuła, Anna, Trifunović, Milana, Subotić, Angelina, Petrić, Marija, Jevremović, Slađana, "The Role of Arabinogalactan Proteins in Morphogenesis of Centaurium erythraea Rafn In Vitro" in The Gentianaceae - Volume 2: Biotechnology and Applications (2015):113-138,
https://doi.org/10.1007/978-3-642-54102-5 . .