TY  - JOUR
AU  - Vidović, Biljana
AU  - Anđelković, Nikola
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Petanović, Radmila
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Rector, Brian G.
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5068
AB  - Simple Summary: Natural enemies of cheatgrass, an invasive plant in the western USA that is
associated with recent increases in destructive wildfires, were sought as part of a classical biological
control program targeting that plant. A population of mites was discovered infesting cheatgrass
plants in central Bulgaria and determined to be a new species using morphological, morphometric
analysis (i.e., measurement of specific body parts) and DNA barcoding (i.e., comparison of DNA
sequences in genes shared by all mite species). Without these modern analytical tools, this mite
would probably have been considered as the same species as another mite that infests other plants.
This new mite species will now be tested to determine if it is suitable for importation and release in
the USA as a biological control agent of cheatgrass.
Abstract: A new species of eriophyoid mite, Aculodes marcelli sp. nov., was discovered on cheatgrass,
Anisantha tectorum (L.) Nevski (syn. Bromus tectorum L.), an annual grass that is native to Eurasia and
Northern Africa. This grass was introduced to North America near the end of the 19th century and
now is widespread and associated with the observed increases in the size, frequency, and intensity of
wildfires in western N. America. In this paper, A. marcelli sp. nov., is morphologically described and
illustrated. Compared with other Aculodes spp., it differs based on morphology and the sequence
of the mitochondrial cytochrome oxidase gene, subunit I (MT-CO1). Results of morphometric
analysis showed clear differentiation between A. marcelli sp. nov., and the most similar congener, A.
altamurgiensis from Taeniatherum caput-medusae. Analysis of MT-CO1 sequence divergence revealed
significant levels of genetic variation (17.7%) and supported the results from the morphometric
analysis; therefore, it is determined that they are two different species. Aculodes marcelli sp. nov., is a
new candidate agent for classical biological control of A. tectorum.
PB  - Basel: MDPI
T2  - Insects
T1  - A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses
IS  - 10
VL  - 13
DO  - 10.3390/insects13100877
SP  - 877
ER  - 

@article{
author = "Vidović, Biljana and Anđelković, Nikola and Jojić, Vida and Cvrković, Tatjana and Petanović, Radmila and Marini, Francesca and Cristofaro, Massimo and Rector, Brian G.",
year = "2022",
abstract = "Simple Summary: Natural enemies of cheatgrass, an invasive plant in the western USA that is
associated with recent increases in destructive wildfires, were sought as part of a classical biological
control program targeting that plant. A population of mites was discovered infesting cheatgrass
plants in central Bulgaria and determined to be a new species using morphological, morphometric
analysis (i.e., measurement of specific body parts) and DNA barcoding (i.e., comparison of DNA
sequences in genes shared by all mite species). Without these modern analytical tools, this mite
would probably have been considered as the same species as another mite that infests other plants.
This new mite species will now be tested to determine if it is suitable for importation and release in
the USA as a biological control agent of cheatgrass.
Abstract: A new species of eriophyoid mite, Aculodes marcelli sp. nov., was discovered on cheatgrass,
Anisantha tectorum (L.) Nevski (syn. Bromus tectorum L.), an annual grass that is native to Eurasia and
Northern Africa. This grass was introduced to North America near the end of the 19th century and
now is widespread and associated with the observed increases in the size, frequency, and intensity of
wildfires in western N. America. In this paper, A. marcelli sp. nov., is morphologically described and
illustrated. Compared with other Aculodes spp., it differs based on morphology and the sequence
of the mitochondrial cytochrome oxidase gene, subunit I (MT-CO1). Results of morphometric
analysis showed clear differentiation between A. marcelli sp. nov., and the most similar congener, A.
altamurgiensis from Taeniatherum caput-medusae. Analysis of MT-CO1 sequence divergence revealed
significant levels of genetic variation (17.7%) and supported the results from the morphometric
analysis; therefore, it is determined that they are two different species. Aculodes marcelli sp. nov., is a
new candidate agent for classical biological control of A. tectorum.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses",
number = "10",
volume = "13",
doi = "10.3390/insects13100877",
pages = "877"
}

Vidović, B., Anđelković, N., Jojić, V., Cvrković, T., Petanović, R., Marini, F., Cristofaro, M.,& Rector, B. G.. (2022). A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses. in Insects
Basel: MDPI., 13(10), 877.
https://doi.org/10.3390/insects13100877

Vidović B, Anđelković N, Jojić V, Cvrković T, Petanović R, Marini F, Cristofaro M, Rector BG. A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses. in Insects. 2022;13(10):877.
doi:10.3390/insects13100877 .

Vidović, Biljana, Anđelković, Nikola, Jojić, Vida, Cvrković, Tatjana, Petanović, Radmila, Marini, Francesca, Cristofaro, Massimo, Rector, Brian G., "A New Aculodes Species (Prostigmata: Eriophyidae) Described from an Invasive Weed by Morphological, Morphometric and DNA Barcode Analyses" in Insects, 13, no. 10 (2022):877,
https://doi.org/10.3390/insects13100877 . .

TY  - CONF
AU  - Marinković, Slavica
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Vidović, Biljana
AU  - Petanović, Radmila
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5310
AB  - Although eriophyoids are considered a group of microscopic organisms with simplified bodies, more than 100 characters and 250 character states related to the body and legs can be used in their systematics. It has been demonstrated that some of these taxonomic characters are more prone to homoplasy. The aim of this study was to evaluate the diagnostic value of 35 morphological traits, analyzed by linear morphometrics, for the delimitation of 14 species from two genera: Cecidophyopsis (C. vermiformis, C. ribis, C. malpighianus, C. hendersoni, C. verilicis, C. rosmarinusis and C. psilaspis) and Cecidophyes (Ce. galii, Ce. rouhollahi, Ce. glaber, Ce. gymnaspis, Ce. nudus, Ce. psilonotus and Ce. lauri). Multivariate analysis of variance (MANOVA) revealed statistically significant interspecies differences (Wilks’λ = 0.0000; F455,4357.726 = 65; P = 0.0000). Canonical variate analysis (CVA) indicated clear segregation of species belonging to Cecidophyopsis and Cecidophyes genera along the CV1 axis. This trend was also supported by the UPGMA cluster analysis. Morphological traits that contributed most to the distinguishing along the CV1 axis were: numbers of dorsal and ventral semiannuli, body width at the level of the f and c2 setae, distances between the 1b - 1a tubercles, distance between d setae and prodorsal shield width. According to the squared Mahalanobis distances, the greatest morphological separation was between C. hendersoni and C. ribis (Cecidophyopsis cluster) and Ce. psilonotus and Ce. lauri (Cecidophyes cluster). In addition, C. vermiformis and C. ribis (Cecidophyopsis cluster), as well as Ce. galii and Ce. glaber (Cecidophyes cluster), showed morphologically very close relationships.
PB  - European Association of Acarologists
C3  - Program and Book of Abstracts: IX Symposium of the European Association of Acarologists: Acarology 1.0 to 2.0: Progress in Changing Times; 2022 Jul 12-15; Bari, Italy
T1  - Delimitation of Cecidophyopsis and Cecidophyes (Eriophyidae: Cecidophyinae) species – linear morphometric methods
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5310
ER  - 

@conference{
author = "Marinković, Slavica and Jojić, Vida and Cvrković, Tatjana and Vidović, Biljana and Petanović, Radmila",
year = "2022",
abstract = "Although eriophyoids are considered a group of microscopic organisms with simplified bodies, more than 100 characters and 250 character states related to the body and legs can be used in their systematics. It has been demonstrated that some of these taxonomic characters are more prone to homoplasy. The aim of this study was to evaluate the diagnostic value of 35 morphological traits, analyzed by linear morphometrics, for the delimitation of 14 species from two genera: Cecidophyopsis (C. vermiformis, C. ribis, C. malpighianus, C. hendersoni, C. verilicis, C. rosmarinusis and C. psilaspis) and Cecidophyes (Ce. galii, Ce. rouhollahi, Ce. glaber, Ce. gymnaspis, Ce. nudus, Ce. psilonotus and Ce. lauri). Multivariate analysis of variance (MANOVA) revealed statistically significant interspecies differences (Wilks’λ = 0.0000; F455,4357.726 = 65; P = 0.0000). Canonical variate analysis (CVA) indicated clear segregation of species belonging to Cecidophyopsis and Cecidophyes genera along the CV1 axis. This trend was also supported by the UPGMA cluster analysis. Morphological traits that contributed most to the distinguishing along the CV1 axis were: numbers of dorsal and ventral semiannuli, body width at the level of the f and c2 setae, distances between the 1b - 1a tubercles, distance between d setae and prodorsal shield width. According to the squared Mahalanobis distances, the greatest morphological separation was between C. hendersoni and C. ribis (Cecidophyopsis cluster) and Ce. psilonotus and Ce. lauri (Cecidophyes cluster). In addition, C. vermiformis and C. ribis (Cecidophyopsis cluster), as well as Ce. galii and Ce. glaber (Cecidophyes cluster), showed morphologically very close relationships.",
publisher = "European Association of Acarologists",
journal = "Program and Book of Abstracts: IX Symposium of the European Association of Acarologists: Acarology 1.0 to 2.0: Progress in Changing Times; 2022 Jul 12-15; Bari, Italy",
title = "Delimitation of Cecidophyopsis and Cecidophyes (Eriophyidae: Cecidophyinae) species – linear morphometric methods",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5310"
}

Marinković, S., Jojić, V., Cvrković, T., Vidović, B.,& Petanović, R.. (2022). Delimitation of Cecidophyopsis and Cecidophyes (Eriophyidae: Cecidophyinae) species – linear morphometric methods. in Program and Book of Abstracts: IX Symposium of the European Association of Acarologists: Acarology 1.0 to 2.0: Progress in Changing Times; 2022 Jul 12-15; Bari, Italy
European Association of Acarologists., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5310

Marinković S, Jojić V, Cvrković T, Vidović B, Petanović R. Delimitation of Cecidophyopsis and Cecidophyes (Eriophyidae: Cecidophyinae) species – linear morphometric methods. in Program and Book of Abstracts: IX Symposium of the European Association of Acarologists: Acarology 1.0 to 2.0: Progress in Changing Times; 2022 Jul 12-15; Bari, Italy. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5310 .

Marinković, Slavica, Jojić, Vida, Cvrković, Tatjana, Vidović, Biljana, Petanović, Radmila, "Delimitation of Cecidophyopsis and Cecidophyes (Eriophyidae: Cecidophyinae) species – linear morphometric methods" in Program and Book of Abstracts: IX Symposium of the European Association of Acarologists: Acarology 1.0 to 2.0: Progress in Changing Times; 2022 Jul 12-15; Bari, Italy (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5310 .

TY  - CONF
AU  - Anđelković, Nikola
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Marinković, Slavica
AU  - Petanović, Radmila
AU  - Vidović, Biljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5311
AB  - Na biljnim vrstama roda Prunus do sada je opisano 13 vrsta eriofidnih grinja roda Aculus. Vrsta Aculus fockeui
(Nalepa & Trouessart) [syn. A. cornutus (Banks)] sa dva trivijalna naziva (šljivina rñasta grinja i grinja srebrnavosti
breskve) je kao ekonomski najznačajnija registrovana na osam komercijalnih vrsta roda Prunus: šljiva, breskva,
kajsija, višnja, trešnja, badem, džanarika i rašeljka. Pravi status kongeneričnih Aculus spp. asociranih sa biljkama roda Prunus je teško odrediti zbog nedostatka novijih detaljnih istraživanja i oskudnih podataka u dosadašnjoj
literaturi. Prema literaturnim podacima, samo se jedno istraživanje bavilo poreñenjem morfoloških karakteristika A.
fockeui sa različitih biljaka domaćina kojim je pokazano prilično variranje morfoloških karaktera izmeñu populacija A.
fockeui sa šljive i višnje.
Cilj ovog istraživanja je razjašnjavanje taksonomskog statusa vrsta roda Aculus sa različitih biljaka domaćina roda
Prunus. U ovom radu su analizirane populacije Aculus spp. sa tri biljke domaćina roda Prunus: P. domestica - šljiva,
P. persica - breskva i P. avium- trešnja.
Za morfometrijsku analizu merena su 23 morfološka karaktera na protoginim ženkama u uzorku od 25-30 individua.
Jednofaktorska multivarijantna analiza varijanse (MANOVA) i kanonijska varijantna analiza (CVA) su korišćene radi
utvrñivanja meñupopulacionih razlika na morfološkom nivou. Ekstrakcija DNK uzoraka (svaki uzorak 1-5 individua) je
rañena pomoću QIAGEN Dneasy Blood and Tissue Kit-a. Region mitohondrijalne DNK (mtDNA) zadužen za sintezu
subjedinice 1 citohrom oksidaze (COI) amplifikovan je pomoću prajmera LCO1490 i HCO2198.
Na osnovu rezultata linearne morfometrije analizirana tri taksona su jasno odvojena (MANOVA: Wilks’λ = 0.0042;
F46,130 = 40.82; P = 0.0000; CVA: duž CV1 ose, koja opisuje 77.3% ukupnih razlika, populacija A. fockeui sa trešnje
se razdvaja od populacija A. fockeui sa breskve i šljive, dok se duž CV2 ose, koja opisuje 22.7% ukupnih razlika,
uočava meñusobno razdvajanje populacija A. fockeui sa breskve i šljive). Poreñenjem sekvenci COI gena izmeñu
populacija A. fockeui sa šljive, breskve i trešnje, nekorigovane p-distance iznosile su 17-20.3%, što ukazuje na
postojanje kompleksa kriptičnih vrsta.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
T1  - Morfološka i molekularna karakterizacija Aculus fockeui sa različitih biljaka domaćina roda Prunus
SP  - 23
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5311
ER  - 

@conference{
author = "Anđelković, Nikola and Jojić, Vida and Cvrković, Tatjana and Marinković, Slavica and Petanović, Radmila and Vidović, Biljana",
year = "2022",
abstract = "Na biljnim vrstama roda Prunus do sada je opisano 13 vrsta eriofidnih grinja roda Aculus. Vrsta Aculus fockeui
(Nalepa & Trouessart) [syn. A. cornutus (Banks)] sa dva trivijalna naziva (šljivina rñasta grinja i grinja srebrnavosti
breskve) je kao ekonomski najznačajnija registrovana na osam komercijalnih vrsta roda Prunus: šljiva, breskva,
kajsija, višnja, trešnja, badem, džanarika i rašeljka. Pravi status kongeneričnih Aculus spp. asociranih sa biljkama roda Prunus je teško odrediti zbog nedostatka novijih detaljnih istraživanja i oskudnih podataka u dosadašnjoj
literaturi. Prema literaturnim podacima, samo se jedno istraživanje bavilo poreñenjem morfoloških karakteristika A.
fockeui sa različitih biljaka domaćina kojim je pokazano prilično variranje morfoloških karaktera izmeñu populacija A.
fockeui sa šljive i višnje.
Cilj ovog istraživanja je razjašnjavanje taksonomskog statusa vrsta roda Aculus sa različitih biljaka domaćina roda
Prunus. U ovom radu su analizirane populacije Aculus spp. sa tri biljke domaćina roda Prunus: P. domestica - šljiva,
P. persica - breskva i P. avium- trešnja.
Za morfometrijsku analizu merena su 23 morfološka karaktera na protoginim ženkama u uzorku od 25-30 individua.
Jednofaktorska multivarijantna analiza varijanse (MANOVA) i kanonijska varijantna analiza (CVA) su korišćene radi
utvrñivanja meñupopulacionih razlika na morfološkom nivou. Ekstrakcija DNK uzoraka (svaki uzorak 1-5 individua) je
rañena pomoću QIAGEN Dneasy Blood and Tissue Kit-a. Region mitohondrijalne DNK (mtDNA) zadužen za sintezu
subjedinice 1 citohrom oksidaze (COI) amplifikovan je pomoću prajmera LCO1490 i HCO2198.
Na osnovu rezultata linearne morfometrije analizirana tri taksona su jasno odvojena (MANOVA: Wilks’λ = 0.0042;
F46,130 = 40.82; P = 0.0000; CVA: duž CV1 ose, koja opisuje 77.3% ukupnih razlika, populacija A. fockeui sa trešnje
se razdvaja od populacija A. fockeui sa breskve i šljive, dok se duž CV2 ose, koja opisuje 22.7% ukupnih razlika,
uočava meñusobno razdvajanje populacija A. fockeui sa breskve i šljive). Poreñenjem sekvenci COI gena izmeñu
populacija A. fockeui sa šljive, breskve i trešnje, nekorigovane p-distance iznosile su 17-20.3%, što ukazuje na
postojanje kompleksa kriptičnih vrsta.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.",
title = "Morfološka i molekularna karakterizacija Aculus fockeui sa različitih biljaka domaćina roda Prunus",
pages = "23-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5311"
}

Anđelković, N., Jojić, V., Cvrković, T., Marinković, S., Petanović, R.,& Vidović, B.. (2022). Morfološka i molekularna karakterizacija Aculus fockeui sa različitih biljaka domaćina roda Prunus. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
Belgrade : Entomological Society of Serbia., 23-24.
https://hdl.handle.net/21.15107/rcub_ibiss_5311

Anđelković N, Jojić V, Cvrković T, Marinković S, Petanović R, Vidović B. Morfološka i molekularna karakterizacija Aculus fockeui sa različitih biljaka domaćina roda Prunus. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.. 2022;:23-24.
https://hdl.handle.net/21.15107/rcub_ibiss_5311 .

Anđelković, Nikola, Jojić, Vida, Cvrković, Tatjana, Marinković, Slavica, Petanović, Radmila, Vidović, Biljana, "Morfološka i molekularna karakterizacija Aculus fockeui sa različitih biljaka domaćina roda Prunus" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia. (2022):23-24,
https://hdl.handle.net/21.15107/rcub_ibiss_5311 .

TY  - CONF
AU  - Anđelković, Nikola
AU  - Rector, Brian G.
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Cvrković, Tatjana
AU  - Jojić, Vida
AU  - De Lillo, Enrico
AU  - Petanović, Radmila
AU  - Vidović, Biljana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5641
AB  - All mites in the family Eriophyidae are obligate herbivores. Many of them are crop pests, some of which can transmit viruses, while others are associated with weeds and have high potential as classical biological control agents. Several eriophyid species have been released as biological control agents but to date, none of them had impact in control of grass targets. So far, about 30 species have been described in the genus Aculodes, out of which most were recorded from grasses (Poaceae). Aculodes altamurgiensis was described by de Lillo & Vidović (2018) from samples of Taeniatherum caput-medusae collected in Italy. Thereafter, cryptic populations of mites from Bromus tectorum were found. The purpose of this study was to clarify the taxonomic status of mites within the genus Aculodes from different host plants.
	Plant samples of T. caput-medusae were collected from Italy and those of B. tectorum were collected from Serbia and Bulgaria. For the morphometric study, 23 morphological traits were measured on 25-27 females of mites from each sample. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were conducted to examine morphological differences between them. 
	Pooled samples of 15–20 whole mites were processed as one sample for DNA extraction, using the Dneasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The barcoding region of the mitochondrial cytochrome oxidase subunit I gene was amplified by polymerase chain reaction (PCR) using a pair of primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were employed to calculate the average genetic distance between species with different host affiliations.
	The results of our morphometric analyses showed general inter-population differences although A. altamurgiensis from T. caput-medusae diverged the most from Aculodes sp. hosted by B. tectorum. Pairwise comparison of the COI distances indicated approximate to 18% of genetic divergence between analyzed populations. These data provide evidence to support the hypothesis that mites associated with T. caput-medusae and B. tectorum are two distinct species.
PB  - Belgrade: Plant Protection Society of Serbia
C3  - Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
T1  - Morphological and molecular characterization of eriophyid mites Aculodes sp. on Taeniatherum caput-medusae and Bromus tectorum (Poaceae)
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5641
ER  - 

@conference{
author = "Anđelković, Nikola and Rector, Brian G. and Marini, Francesca and Cristofaro, Massimo and Cvrković, Tatjana and Jojić, Vida and De Lillo, Enrico and Petanović, Radmila and Vidović, Biljana",
year = "2019",
abstract = "All mites in the family Eriophyidae are obligate herbivores. Many of them are crop pests, some of which can transmit viruses, while others are associated with weeds and have high potential as classical biological control agents. Several eriophyid species have been released as biological control agents but to date, none of them had impact in control of grass targets. So far, about 30 species have been described in the genus Aculodes, out of which most were recorded from grasses (Poaceae). Aculodes altamurgiensis was described by de Lillo & Vidović (2018) from samples of Taeniatherum caput-medusae collected in Italy. Thereafter, cryptic populations of mites from Bromus tectorum were found. The purpose of this study was to clarify the taxonomic status of mites within the genus Aculodes from different host plants.
	Plant samples of T. caput-medusae were collected from Italy and those of B. tectorum were collected from Serbia and Bulgaria. For the morphometric study, 23 morphological traits were measured on 25-27 females of mites from each sample. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were conducted to examine morphological differences between them. 
	Pooled samples of 15–20 whole mites were processed as one sample for DNA extraction, using the Dneasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The barcoding region of the mitochondrial cytochrome oxidase subunit I gene was amplified by polymerase chain reaction (PCR) using a pair of primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were employed to calculate the average genetic distance between species with different host affiliations.
	The results of our morphometric analyses showed general inter-population differences although A. altamurgiensis from T. caput-medusae diverged the most from Aculodes sp. hosted by B. tectorum. Pairwise comparison of the COI distances indicated approximate to 18% of genetic divergence between analyzed populations. These data provide evidence to support the hypothesis that mites associated with T. caput-medusae and B. tectorum are two distinct species.",
publisher = "Belgrade: Plant Protection Society of Serbia",
journal = "Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia",
title = "Morphological and molecular characterization of eriophyid mites Aculodes sp. on Taeniatherum caput-medusae and Bromus tectorum (Poaceae)",
pages = "166",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5641"
}

Anđelković, N., Rector, B. G., Marini, F., Cristofaro, M., Cvrković, T., Jojić, V., De Lillo, E., Petanović, R.,& Vidović, B.. (2019). Morphological and molecular characterization of eriophyid mites Aculodes sp. on Taeniatherum caput-medusae and Bromus tectorum (Poaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
Belgrade: Plant Protection Society of Serbia., 166.
https://hdl.handle.net/21.15107/rcub_ibiss_5641

Anđelković N, Rector BG, Marini F, Cristofaro M, Cvrković T, Jojić V, De Lillo E, Petanović R, Vidović B. Morphological and molecular characterization of eriophyid mites Aculodes sp. on Taeniatherum caput-medusae and Bromus tectorum (Poaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia. 2019;:166.
https://hdl.handle.net/21.15107/rcub_ibiss_5641 .

Anđelković, Nikola, Rector, Brian G., Marini, Francesca, Cristofaro, Massimo, Cvrković, Tatjana, Jojić, Vida, De Lillo, Enrico, Petanović, Radmila, Vidović, Biljana, "Morphological and molecular characterization of eriophyid mites Aculodes sp. on Taeniatherum caput-medusae and Bromus tectorum (Poaceae)" in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia (2019):166,
https://hdl.handle.net/21.15107/rcub_ibiss_5641 .

TY  - CONF
AU  - Vidović, Biljana
AU  - Weyl, Philip
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Cvrković, Tatjana
AU  - Jojić, Vida
AU  - Petanović, Radmila
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5639
AB  - In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.
PB  - Belgrade: Plant Protection Society of Serbia
C3  - Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
T1  - Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5639
ER  - 

@conference{
author = "Vidović, Biljana and Weyl, Philip and Marini, Francesca and Cristofaro, Massimo and Cvrković, Tatjana and Jojić, Vida and Petanović, Radmila",
year = "2019",
abstract = "In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.",
publisher = "Belgrade: Plant Protection Society of Serbia",
journal = "Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia",
title = "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5639"
}

Vidović, B., Weyl, P., Marini, F., Cristofaro, M., Cvrković, T., Jojić, V.,& Petanović, R.. (2019). Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
Belgrade: Plant Protection Society of Serbia., 165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639

Vidović B, Weyl P, Marini F, Cristofaro M, Cvrković T, Jojić V, Petanović R. Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia. 2019;:165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .

Vidović, Biljana, Weyl, Philip, Marini, Francesca, Cristofaro, Massimo, Cvrković, Tatjana, Jojić, Vida, Petanović, Radmila, "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)" in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia (2019):165,
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .

TY  - JOUR
AU  - Živković, Zlata
AU  - Vidović, Biljana
AU  - Jojić, Vida
AU  - Cvrković, Tatjana
AU  - Petanović, Radmila
PY  - 2017
UR  - http://link.springer.com/10.1007/s10493-017-0128-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2750
AB  - We examined morphological and genetic differences among Aceria spp. inhabiting six Brassicaceae species in Serbia. Five of them have been already mentioned in the literature as original or alternate hosts of Aceria spp. (Berteroa incana (L.) DC., Capsella bursa-pastoris (L.) Medik., Cardamine hirsuta L., Lepidium draba L. and Sisymbrium orientale L.), whereas Aurinia petraea (Ard.) Schur was registered here for the first time as a host for Aceria sp. Results of morphometric analyses indicated clear differentiation of Aceria spp. from B. incana, L. draba, A. petraea and S. orientale, whereas the other two entities were less diverse and clustered together. Molecular analyses indicated that the average mean divergence over all sequence pairs was 18.3% (11.8–25.9%) and disclosed that the observed mtCOI distances between the six host-associated Aceria entities are large enough to represent differences between species. The Aceria sp. hosted by B. incana was morphologically and genetically most distinctive, whereas discrepancy between phenetic and phylogenetic relationships was found in the position of Aceria sp. hosted by L. draba when compared with those inhabiting C. hirsuta, S. orientale and C. bursa-pastoris.
T2  - Experimental and Applied Acarology
T1  - Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia
IS  - 4
VL  - 71
DO  - 10.1007/s10493-017-0128-8
SP  - 329
EP  - 343
ER  - 

@article{
author = "Živković, Zlata and Vidović, Biljana and Jojić, Vida and Cvrković, Tatjana and Petanović, Radmila",
year = "2017",
abstract = "We examined morphological and genetic differences among Aceria spp. inhabiting six Brassicaceae species in Serbia. Five of them have been already mentioned in the literature as original or alternate hosts of Aceria spp. (Berteroa incana (L.) DC., Capsella bursa-pastoris (L.) Medik., Cardamine hirsuta L., Lepidium draba L. and Sisymbrium orientale L.), whereas Aurinia petraea (Ard.) Schur was registered here for the first time as a host for Aceria sp. Results of morphometric analyses indicated clear differentiation of Aceria spp. from B. incana, L. draba, A. petraea and S. orientale, whereas the other two entities were less diverse and clustered together. Molecular analyses indicated that the average mean divergence over all sequence pairs was 18.3% (11.8–25.9%) and disclosed that the observed mtCOI distances between the six host-associated Aceria entities are large enough to represent differences between species. The Aceria sp. hosted by B. incana was morphologically and genetically most distinctive, whereas discrepancy between phenetic and phylogenetic relationships was found in the position of Aceria sp. hosted by L. draba when compared with those inhabiting C. hirsuta, S. orientale and C. bursa-pastoris.",
journal = "Experimental and Applied Acarology",
title = "Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia",
number = "4",
volume = "71",
doi = "10.1007/s10493-017-0128-8",
pages = "329-343"
}

Živković, Z., Vidović, B., Jojić, V., Cvrković, T.,& Petanović, R.. (2017). Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia. in Experimental and Applied Acarology, 71(4), 329-343.
https://doi.org/10.1007/s10493-017-0128-8

Živković Z, Vidović B, Jojić V, Cvrković T, Petanović R. Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia. in Experimental and Applied Acarology. 2017;71(4):329-343.
doi:10.1007/s10493-017-0128-8 .

Živković, Zlata, Vidović, Biljana, Jojić, Vida, Cvrković, Tatjana, Petanović, Radmila, "Phenetic and phylogenetic relationships among Aceria spp. (Acari: Eriophyoidea) inhabiting species within the family Brassicaceae in Serbia" in Experimental and Applied Acarology, 71, no. 4 (2017):329-343,
https://doi.org/10.1007/s10493-017-0128-8 . .