TY  - JOUR
AU  - Szekeres, József
AU  - Beermann, Arne
AU  - Neubauer, Thomas
AU  - Ocadlik, Miroslav
AU  - Paunović, Momir
AU  - Raković, Maja
AU  - Csányi, Béla
AU  - Varga, András
AU  - Weigand, Alexander
AU  - Wilke, Thomas
AU  - Fehér, Zoltán
PY  - 2022
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642200006S
UR  - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/7492
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5183
AB  - We examined the spread and distribution in the Danube River of a new alien gastropod species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae: Caspiinae). First findings of this species for Hungary, Slovakia, Romania, Bulgaria and Serbia are presented. Clathrocaspia knipowitschii was initially found in 2013 in the Iron Gate stretch of the Danube River at the border between Romania and Serbia. In 2019 and 2020, the species was found at several sites in the lower Danube in Romania, Serbia and Bulgaria, and also upstream in the middle Hungarian Danube in high population densities. The species appears to have spread along more than 800 km in six years. This finding together with the available abundance data indicates that C. knipowitschii is potentially an invasive species, but further observations are needed.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River
IS  - 1
VL  - 74
DO  - 10.2298/ABS220211006S
SP  - 81
EP  - 89
ER  - 

@article{
author = "Szekeres, József and Beermann, Arne and Neubauer, Thomas and Ocadlik, Miroslav and Paunović, Momir and Raković, Maja and Csányi, Béla and Varga, András and Weigand, Alexander and Wilke, Thomas and Fehér, Zoltán",
year = "2022",
abstract = "We examined the spread and distribution in the Danube River of a new alien gastropod species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae: Caspiinae). First findings of this species for Hungary, Slovakia, Romania, Bulgaria and Serbia are presented. Clathrocaspia knipowitschii was initially found in 2013 in the Iron Gate stretch of the Danube River at the border between Romania and Serbia. In 2019 and 2020, the species was found at several sites in the lower Danube in Romania, Serbia and Bulgaria, and also upstream in the middle Hungarian Danube in high population densities. The species appears to have spread along more than 800 km in six years. This finding together with the available abundance data indicates that C. knipowitschii is potentially an invasive species, but further observations are needed.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River",
number = "1",
volume = "74",
doi = "10.2298/ABS220211006S",
pages = "81-89"
}

Szekeres, J., Beermann, A., Neubauer, T., Ocadlik, M., Paunović, M., Raković, M., Csányi, B., Varga, A., Weigand, A., Wilke, T.,& Fehér, Z.. (2022). Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 74(1), 81-89.
https://doi.org/10.2298/ABS220211006S

Szekeres J, Beermann A, Neubauer T, Ocadlik M, Paunović M, Raković M, Csányi B, Varga A, Weigand A, Wilke T, Fehér Z. Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River. in Archives of Biological Sciences. 2022;74(1):81-89.
doi:10.2298/ABS220211006S .

Szekeres, József, Beermann, Arne, Neubauer, Thomas, Ocadlik, Miroslav, Paunović, Momir, Raković, Maja, Csányi, Béla, Varga, András, Weigand, Alexander, Wilke, Thomas, Fehér, Zoltán, "Rapid spread of a new alien and potentially invasive species, Clathrocaspia knipowitschii (Makarov, 1938) (Gastropoda: Hydrobiidae), in the Danube River" in Archives of Biological Sciences, 74, no. 1 (2022):81-89,
https://doi.org/10.2298/ABS220211006S . .

TY  - JOUR
AU  - Pont, Didier
AU  - Meulenbroek, Paul
AU  - Bammer, Vincenz
AU  - Dejean, Tony
AU  - Erős, Tibor
AU  - Jean, Pauline
AU  - Lenhardt, Mirjana
AU  - Nagel, Christoffer
AU  - Pekarik, Ladislav
AU  - Schabuss, Michael
AU  - Stoeckle, Bernhard C
AU  - Stoica, Elena
AU  - Zornig, Horst
AU  - Weigand, Alexander
AU  - Valentini, Alice
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5163
AB  - Environmental DNA (eDNA) metabarcoding is an effective method for studying fish communities but allows only an estimation of relative species abundance (density/biomass). Here, we combine metabarcoding with an estimation of the total abundance of eDNA amplified by our universal marker (teleo) using a quantitative (q)PCR approach to infer the absolute abundance of fish species. We carried out a 2850-km eDNA survey within the Danube catchment using a spatial integrative sampling protocol coupled with traditional electrofishing for fish biomass and density estimation. Total fish eDNA concentrations and total fish abundance were highly correlated. The correlation between eDNA concentrations per taxon and absolute specific abundance was of comparable strength when all sites were pooled and remained significant when the sites were considered separately. Furthermore, a nonlinear mixed model showed that species richness was underestimated when the amount of teleo-DNA extracted from a sample was below a threshold of 0.65 × 106 copies of eDNA. This result, combined with the decrease in teleo-DNA concentration by several orders of magnitude with river size, highlights the need to increase sampling effort in large rivers. Our results provide a comprehensive description of longitudinal changes in fish communities and underline our combined metabarcoding/qPCR approach for biomonitoring and bioassessment surveys when a rough estimate of absolute species abundance is sufficient.
PB  - Hoboken: Wiley
T2  - Molecular Ecology Resources
T1  - Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR.
DO  - 10.1111/1755-0998.13715
ER  - 

@article{
author = "Pont, Didier and Meulenbroek, Paul and Bammer, Vincenz and Dejean, Tony and Erős, Tibor and Jean, Pauline and Lenhardt, Mirjana and Nagel, Christoffer and Pekarik, Ladislav and Schabuss, Michael and Stoeckle, Bernhard C and Stoica, Elena and Zornig, Horst and Weigand, Alexander and Valentini, Alice",
year = "2022",
abstract = "Environmental DNA (eDNA) metabarcoding is an effective method for studying fish communities but allows only an estimation of relative species abundance (density/biomass). Here, we combine metabarcoding with an estimation of the total abundance of eDNA amplified by our universal marker (teleo) using a quantitative (q)PCR approach to infer the absolute abundance of fish species. We carried out a 2850-km eDNA survey within the Danube catchment using a spatial integrative sampling protocol coupled with traditional electrofishing for fish biomass and density estimation. Total fish eDNA concentrations and total fish abundance were highly correlated. The correlation between eDNA concentrations per taxon and absolute specific abundance was of comparable strength when all sites were pooled and remained significant when the sites were considered separately. Furthermore, a nonlinear mixed model showed that species richness was underestimated when the amount of teleo-DNA extracted from a sample was below a threshold of 0.65 × 106 copies of eDNA. This result, combined with the decrease in teleo-DNA concentration by several orders of magnitude with river size, highlights the need to increase sampling effort in large rivers. Our results provide a comprehensive description of longitudinal changes in fish communities and underline our combined metabarcoding/qPCR approach for biomonitoring and bioassessment surveys when a rough estimate of absolute species abundance is sufficient.",
publisher = "Hoboken: Wiley",
journal = "Molecular Ecology Resources",
title = "Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR.",
doi = "10.1111/1755-0998.13715"
}

Pont, D., Meulenbroek, P., Bammer, V., Dejean, T., Erős, T., Jean, P., Lenhardt, M., Nagel, C., Pekarik, L., Schabuss, M., Stoeckle, B. C., Stoica, E., Zornig, H., Weigand, A.,& Valentini, A.. (2022). Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR.. in Molecular Ecology Resources
Hoboken: Wiley..
https://doi.org/10.1111/1755-0998.13715

Pont D, Meulenbroek P, Bammer V, Dejean T, Erős T, Jean P, Lenhardt M, Nagel C, Pekarik L, Schabuss M, Stoeckle BC, Stoica E, Zornig H, Weigand A, Valentini A. Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR.. in Molecular Ecology Resources. 2022;.
doi:10.1111/1755-0998.13715 .

Pont, Didier, Meulenbroek, Paul, Bammer, Vincenz, Dejean, Tony, Erős, Tibor, Jean, Pauline, Lenhardt, Mirjana, Nagel, Christoffer, Pekarik, Ladislav, Schabuss, Michael, Stoeckle, Bernhard C, Stoica, Elena, Zornig, Horst, Weigand, Alexander, Valentini, Alice, "Quantitative monitoring of diverse fish communities on a large scale combining eDNA metabarcoding and qPCR." in Molecular Ecology Resources (2022),
https://doi.org/10.1111/1755-0998.13715 . .

TY  - CONF
AU  - Trichkova, Teodora
AU  - Csányi, Béla
AU  - Weiperth, András
AU  - Zorić, Katarina
AU  - Bammer, Vinzenz
AU  - Borza, Péter
AU  - Weigand, Alexander
AU  - Cardoso, Ana Cristina
AU  - Očadlik, Miroslav
AU  - Bubíková, Kateřina
AU  - Stanković, Igor
AU  - Todorov, Milcho
AU  - Botev, Ivan
AU  - Kenderov, Marian
AU  - Hubenov, Zdravko
AU  - Paunović, Momir
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4785
AB  - The co-existential pattern of native and alien species was investigated along the Danube River Basin (DRB) during JDS4 (2019). A total of 51 JDS4 sites was sampled in the entire Danube River, and additional sites in the Lower Danube. The standard operational procedures adopted by ICPDR, and – for the first time – eDNA-based tools were used for the sampling. A smartphone application ‘Invasive Alien Species in Europe’ developed by the European Commission’s Joint Research Centre and specifically updated to include invasive alien species concerning the DRB was additionally applied for data collection.
Six alien aquatic plants, 35 benthic macroinvertebrates and 17 fish species were recorded in DRB during the survey. Two groups of invasive species should be mentioned: 1) Ponto-Caspian species that do not have to cross very strong hydro- and geographical barriers during their spreading; and 2) Species that cross large geographical distances to arrive to the DRB (originated from North America and Asia). An overall decrease of the former abundance of Corbicula fluminea was recognised in some Danube sections. An extreme large amount of Ponto-Caspian amphipods was detected in the Upper Danube: Echinogammarus sp. and Gammaridae gen. sp., representing one third of the overall individual number of the macroinvertebrates detected in the Upper Danube (Austria). New Decapoda species (Procambarus clarkii, Pacifastacus leniusculus) appeared at new locations of the Middle Danube, indicating their recent spreading. A new Ponto-Caspian snail species (Clathrocaspia knipowitschii) was distributed along extended sections of the Lower and Middle Danube. The magnificent bryozoan Pectinatella magnifica was recorded for the first time in the Bulgarian shoreline zone of the Danube River. The (e)DNA-based detection revealed the presence of 5 macroinvertebrate species which were not recorded by other methods. The level of biocontamination of the Danube River was estimated as moderate to high, with higher levels for the Upper (high to severe biocontamination) and Middle
41
Danube (moderate to high biocontamination), in comparison to the Lower Danube (low biocontamination). The results show that DRB is under considerable influence of biological invasions. The number of identified alien species has increased over three times since 2007. The (e)DNA-based method has proved to be an effective additional tool in aquatic IAS monitoring. The smartphone app may greatly facilitate the access and update of IAS records for management and control purposes and contribute to IAS awareness raising in the Danube countries by involving actively the citizens in future surveys.
PB  - Neuburg : Aueninstitut Neuburg
C3  - Proceedings of the 43rd IAD Conference Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Invasive alien species in the Danube River Basin: Results of the JDS4
SP  - 40
EP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4785
ER  - 

@conference{
author = "Trichkova, Teodora and Csányi, Béla and Weiperth, András and Zorić, Katarina and Bammer, Vinzenz and Borza, Péter and Weigand, Alexander and Cardoso, Ana Cristina and Očadlik, Miroslav and Bubíková, Kateřina and Stanković, Igor and Todorov, Milcho and Botev, Ivan and Kenderov, Marian and Hubenov, Zdravko and Paunović, Momir",
year = "2021",
abstract = "The co-existential pattern of native and alien species was investigated along the Danube River Basin (DRB) during JDS4 (2019). A total of 51 JDS4 sites was sampled in the entire Danube River, and additional sites in the Lower Danube. The standard operational procedures adopted by ICPDR, and – for the first time – eDNA-based tools were used for the sampling. A smartphone application ‘Invasive Alien Species in Europe’ developed by the European Commission’s Joint Research Centre and specifically updated to include invasive alien species concerning the DRB was additionally applied for data collection.
Six alien aquatic plants, 35 benthic macroinvertebrates and 17 fish species were recorded in DRB during the survey. Two groups of invasive species should be mentioned: 1) Ponto-Caspian species that do not have to cross very strong hydro- and geographical barriers during their spreading; and 2) Species that cross large geographical distances to arrive to the DRB (originated from North America and Asia). An overall decrease of the former abundance of Corbicula fluminea was recognised in some Danube sections. An extreme large amount of Ponto-Caspian amphipods was detected in the Upper Danube: Echinogammarus sp. and Gammaridae gen. sp., representing one third of the overall individual number of the macroinvertebrates detected in the Upper Danube (Austria). New Decapoda species (Procambarus clarkii, Pacifastacus leniusculus) appeared at new locations of the Middle Danube, indicating their recent spreading. A new Ponto-Caspian snail species (Clathrocaspia knipowitschii) was distributed along extended sections of the Lower and Middle Danube. The magnificent bryozoan Pectinatella magnifica was recorded for the first time in the Bulgarian shoreline zone of the Danube River. The (e)DNA-based detection revealed the presence of 5 macroinvertebrate species which were not recorded by other methods. The level of biocontamination of the Danube River was estimated as moderate to high, with higher levels for the Upper (high to severe biocontamination) and Middle
41
Danube (moderate to high biocontamination), in comparison to the Lower Danube (low biocontamination). The results show that DRB is under considerable influence of biological invasions. The number of identified alien species has increased over three times since 2007. The (e)DNA-based method has proved to be an effective additional tool in aquatic IAS monitoring. The smartphone app may greatly facilitate the access and update of IAS records for management and control purposes and contribute to IAS awareness raising in the Danube countries by involving actively the citizens in future surveys.",
publisher = "Neuburg : Aueninstitut Neuburg",
journal = "Proceedings of the 43rd IAD Conference Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Invasive alien species in the Danube River Basin: Results of the JDS4",
pages = "40-41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4785"
}

Trichkova, T., Csányi, B., Weiperth, A., Zorić, K., Bammer, V., Borza, P., Weigand, A., Cardoso, A. C., Očadlik, M., Bubíková, K., Stanković, I., Todorov, M., Botev, I., Kenderov, M., Hubenov, Z.,& Paunović, M.. (2021). Invasive alien species in the Danube River Basin: Results of the JDS4. in Proceedings of the 43rd IAD Conference Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Neuburg : Aueninstitut Neuburg., 40-41.
https://hdl.handle.net/21.15107/rcub_ibiss_4785

Trichkova T, Csányi B, Weiperth A, Zorić K, Bammer V, Borza P, Weigand A, Cardoso AC, Očadlik M, Bubíková K, Stanković I, Todorov M, Botev I, Kenderov M, Hubenov Z, Paunović M. Invasive alien species in the Danube River Basin: Results of the JDS4. in Proceedings of the 43rd IAD Conference Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;:40-41.
https://hdl.handle.net/21.15107/rcub_ibiss_4785 .

Trichkova, Teodora, Csányi, Béla, Weiperth, András, Zorić, Katarina, Bammer, Vinzenz, Borza, Péter, Weigand, Alexander, Cardoso, Ana Cristina, Očadlik, Miroslav, Bubíková, Kateřina, Stanković, Igor, Todorov, Milcho, Botev, Ivan, Kenderov, Marian, Hubenov, Zdravko, Paunović, Momir, "Invasive alien species in the Danube River Basin: Results of the JDS4" in Proceedings of the 43rd IAD Conference Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021):40-41,
https://hdl.handle.net/21.15107/rcub_ibiss_4785 .