TY  - JOUR
AU  - Jelača, Sanja
AU  - Jovanović, Ivan
AU  - Bovan, Dijana
AU  - Jovanović, Marina Z.
AU  - Jurišević, Milena M.
AU  - Dunđerović, Duško
AU  - Dajić-Stevanović, Zora
AU  - Arsenijević, Nebojša
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6575
AB  - Ethnomedicinal records have long mentioned the historical usage of Alchemilla vulgaris L. in folk medicine, particularly for the treatment of gynecological issues. Building on this ethnomedicinal knowledge regarding female illnesses, the aim of this research was to evaluate the impact of ethanolic extract of A. vulgaris on mouse breast cancer cells (4T1) in vitro and in vivo, in addition to its effect on the immune compartment in the tumor microenvironment. Behind viability decrease of 4T1 cells induced by treatment with A. vulgaris extract was strong inhibition of cell proliferation accompanied by caspase-dependent apoptosis and autophagic cell death. Observed changes in 4T1 cell culture after treatment were well orchestrated and led to a reduction in metastatic potential through weakened adhesion, invasion, migration, and colony-forming abilities in vitro. Enhanced intracellular production of reactive oxygen and nitrogen species promoted by the treatment might interfere with all the observed effects. Apart from the direct effect on tumor cells, the A. vulgaris extract significantly reduced tumor growth in the solid orthotropic mammary carcinoma model through restitution of efficient local and systemic immune response reflected in enhanced antigen-presenting potential of dendritic cells (DCs) as well as the extent and activity of effector T cells.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response
IS  - 3
VL  - 17
DO  - 10.3390/ph17030286
SP  - 286
ER  - 

@article{
author = "Jelača, Sanja and Jovanović, Ivan and Bovan, Dijana and Jovanović, Marina Z. and Jurišević, Milena M. and Dunđerović, Duško and Dajić-Stevanović, Zora and Arsenijević, Nebojša and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2024",
abstract = "Ethnomedicinal records have long mentioned the historical usage of Alchemilla vulgaris L. in folk medicine, particularly for the treatment of gynecological issues. Building on this ethnomedicinal knowledge regarding female illnesses, the aim of this research was to evaluate the impact of ethanolic extract of A. vulgaris on mouse breast cancer cells (4T1) in vitro and in vivo, in addition to its effect on the immune compartment in the tumor microenvironment. Behind viability decrease of 4T1 cells induced by treatment with A. vulgaris extract was strong inhibition of cell proliferation accompanied by caspase-dependent apoptosis and autophagic cell death. Observed changes in 4T1 cell culture after treatment were well orchestrated and led to a reduction in metastatic potential through weakened adhesion, invasion, migration, and colony-forming abilities in vitro. Enhanced intracellular production of reactive oxygen and nitrogen species promoted by the treatment might interfere with all the observed effects. Apart from the direct effect on tumor cells, the A. vulgaris extract significantly reduced tumor growth in the solid orthotropic mammary carcinoma model through restitution of efficient local and systemic immune response reflected in enhanced antigen-presenting potential of dendritic cells (DCs) as well as the extent and activity of effector T cells.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response",
number = "3",
volume = "17",
doi = "10.3390/ph17030286",
pages = "286"
}

Jelača, S., Jovanović, I., Bovan, D., Jovanović, M. Z., Jurišević, M. M., Dunđerović, D., Dajić-Stevanović, Z., Arsenijević, N., Mijatović, S.,& Maksimović-Ivanić, D.. (2024). Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response. in Pharmaceuticals
Basel: MDPI., 17(3), 286.
https://doi.org/10.3390/ph17030286

Jelača S, Jovanović I, Bovan D, Jovanović MZ, Jurišević MM, Dunđerović D, Dajić-Stevanović Z, Arsenijević N, Mijatović S, Maksimović-Ivanić D. Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response. in Pharmaceuticals. 2024;17(3):286.
doi:10.3390/ph17030286 .

Jelača, Sanja, Jovanović, Ivan, Bovan, Dijana, Jovanović, Marina Z., Jurišević, Milena M., Dunđerović, Duško, Dajić-Stevanović, Zora, Arsenijević, Nebojša, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response" in Pharmaceuticals, 17, no. 3 (2024):286,
https://doi.org/10.3390/ph17030286 . .

TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić-Stevanović, Zora
AU  - Jovanović, Ivan
AU  - Pavlović, Sladjana
AU  - Gajović, Nevena
AU  - Mijatović, Sanja
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5297
AB  - Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.
PB  - EFIS Young Immunologist Network (yEFIS Network)
C3  - Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
T1  - Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model
DO  - 10.1002/eji.202270200
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5297
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić-Stevanović, Zora and Jovanović, Ivan and Pavlović, Sladjana and Gajović, Nevena and Mijatović, Sanja and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.",
publisher = "EFIS Young Immunologist Network (yEFIS Network)",
journal = "Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany",
title = "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model",
doi = "10.1002/eji.202270200",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5297"
}

Jelača, S., Drača, D., Dajić-Stevanović, Z., Jovanović, I., Pavlović, S., Gajović, N., Mijatović, S., Arsenijević, N.,& Maksimović-Ivanić, D.. (2022). Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
EFIS Young Immunologist Network (yEFIS Network)., 69.
https://doi.org/10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297

Jelača S, Drača D, Dajić-Stevanović Z, Jovanović I, Pavlović S, Gajović N, Mijatović S, Arsenijević N, Maksimović-Ivanić D. Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany. 2022;:69.
doi:10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

Jelača, Sanja, Drača, Dijana, Dajić-Stevanović, Zora, Jovanović, Ivan, Pavlović, Sladjana, Gajović, Nevena, Mijatović, Sanja, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model" in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany (2022):69,
https://doi.org/10.1002/eji.202270200 .,
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

TY  - CONF
AU  - Dajić-Stevanović, Zora
AU  - Arsenijević, Nebojša
AU  - Kolašinac, Stefan
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Tanić, Nikola
AU  - Tanić, Nasta
AU  - Kanjevac, Tatjana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5293
AB  - Secondary metabolites of plants are known as efficient bioactive compounds used in
prophylaxes and treatment of different disorders and diseases. Plants products have a
long history of use in the treatment of cancer. There are very interesting reports from
ethnobotanical studies, including those from the Balkan region, known for high diversity
of medicinal plants, highlighting the use of various plant drugs as anticancer
agents. More than 3000 plant species were listed as possible anticancer agents. In the last
few decades it was shown that some of plant metabolites exhibit potent and promising
therapeutic effects in cancer treatment. Surprisingly, more than 10,000 phytochemicals
have been identified and used in cancer treatment due to their anti-cancer properties.
The most researched are those belonging to alkaloids, flavonoids, lignans, condensed
tannins, terpenoids (components of essential oils), and others. Additionally, bioactive
compounds could synergistically increase the efficiency of anti-cancer drugs and reduce
their toxic effects. Many successful anti-cancer drugs currently in use, or their
analogues, are plant derived and many more are under clinical trials. This review aims
to address the most reported plants used for cancer treatment in relation to their major
bioactive compounds. Furthermore, possible mechanisms of anticancer activity of
selected plant metabolites will be discussed, including apoptotic pathways, inhibition
of Nuclear Factor-κB (NF-κB), modulation of Wnt/beta-catenin signaling, autophagy,
sensitization of multidrug resistant cancer cells, prevention of cancer cell metastasis
and epigenetic regulation. It is well assumed that high antioxidant activity and anti-inflamamtory
effects of herbal drugs are associated with the anticancer activity. In our
study, several well-known and several autochtonous medicinal plants which expressed
the anticancer potential, will be additionally presented.
AB  - Секундарни метаболити биљака се због свог високог биоактивног дејства ко-
ристе у профилакси и лечењу различитих обољења. Биљне компоненте имају дугу
историју коришћења у третману канцера. Постоје многи занимљиви подаци из
етноботаничких студија, укључујући и оне са подручја Балкана, иначе подруч-
ја познатог по високом диверзитету лековитог биља, о употреби различитих
анти-канцерогених биљака. Више од 3000 биљних врста са анти-канцерогеним
потенцијалом је споменуто у светској литератури. Последњих деценија је пока-
зано да биљни метоболити испољавају високе и обећавајуће терапијске ефекте у
третману канцерогених обољења. Чак 10.000 биоактивних биљних компоненти је
идентификовано као потенцијално анти-канцерогених, међу којима су највише
проучавани алкалоиди, флавоноиди, лигнани, кондензовани танини, терпеноиди
(компоненте етарских уља), и други. Многа биоактивна биљна једињења испо-
љавају синергистичка дејства приликом коришћења са стандардним анти-канце-
рогеним терапеутицима, при чему могу умањити и негативне токсичне ефекте
цитостатика. Наш рад има за циљ да представи најчешће коришћене биљне врсте
у третману канцера у вези са хемијским саставом њихових дрога, тј., доминант-
ним биоактивним компонентама. Такође, биће размотрени и потенцијални ан-
тиканцерогени механизми деловања важнијих биљних секундарних метаболита,
као и њихови ефекти на нпр. апоптозу, инхибицију NF-κB фактора, модулацију
Wnt-бета-катенин сигналних путева, аутофагију, као и позитивне утицаје на ре-
зистентност канцерогених ћелија на различите хемијске агенсе и епигенетичке
одговоре. Иначе је познато да су висока антиоксидативна активност и анти-ин-
фламацијски ефекти биљних дрога повезани са њиховим анти-канцерогеним деј-
ством. Неколико оваквих, у свету добро проучених, као и неколико аутохтоних
врста са нашег подручја, ће бити посебно представљено.
PB  - Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
C3  - Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
T1  - Plant bioactive compounds in cancer treatment: myth or hope?
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5293
ER  - 

@conference{
author = "Dajić-Stevanović, Zora and Arsenijević, Nebojša and Kolašinac, Stefan and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Tanić, Nikola and Tanić, Nasta and Kanjevac, Tatjana",
year = "2022",
abstract = "Secondary metabolites of plants are known as efficient bioactive compounds used in
prophylaxes and treatment of different disorders and diseases. Plants products have a
long history of use in the treatment of cancer. There are very interesting reports from
ethnobotanical studies, including those from the Balkan region, known for high diversity
of medicinal plants, highlighting the use of various plant drugs as anticancer
agents. More than 3000 plant species were listed as possible anticancer agents. In the last
few decades it was shown that some of plant metabolites exhibit potent and promising
therapeutic effects in cancer treatment. Surprisingly, more than 10,000 phytochemicals
have been identified and used in cancer treatment due to their anti-cancer properties.
The most researched are those belonging to alkaloids, flavonoids, lignans, condensed
tannins, terpenoids (components of essential oils), and others. Additionally, bioactive
compounds could synergistically increase the efficiency of anti-cancer drugs and reduce
their toxic effects. Many successful anti-cancer drugs currently in use, or their
analogues, are plant derived and many more are under clinical trials. This review aims
to address the most reported plants used for cancer treatment in relation to their major
bioactive compounds. Furthermore, possible mechanisms of anticancer activity of
selected plant metabolites will be discussed, including apoptotic pathways, inhibition
of Nuclear Factor-κB (NF-κB), modulation of Wnt/beta-catenin signaling, autophagy,
sensitization of multidrug resistant cancer cells, prevention of cancer cell metastasis
and epigenetic regulation. It is well assumed that high antioxidant activity and anti-inflamamtory
effects of herbal drugs are associated with the anticancer activity. In our
study, several well-known and several autochtonous medicinal plants which expressed
the anticancer potential, will be additionally presented., Секундарни метаболити биљака се због свог високог биоактивног дејства ко-
ристе у профилакси и лечењу различитих обољења. Биљне компоненте имају дугу
историју коришћења у третману канцера. Постоје многи занимљиви подаци из
етноботаничких студија, укључујући и оне са подручја Балкана, иначе подруч-
ја познатог по високом диверзитету лековитог биља, о употреби различитих
анти-канцерогених биљака. Више од 3000 биљних врста са анти-канцерогеним
потенцијалом је споменуто у светској литератури. Последњих деценија је пока-
зано да биљни метоболити испољавају високе и обећавајуће терапијске ефекте у
третману канцерогених обољења. Чак 10.000 биоактивних биљних компоненти је
идентификовано као потенцијално анти-канцерогених, међу којима су највише
проучавани алкалоиди, флавоноиди, лигнани, кондензовани танини, терпеноиди
(компоненте етарских уља), и други. Многа биоактивна биљна једињења испо-
љавају синергистичка дејства приликом коришћења са стандардним анти-канце-
рогеним терапеутицима, при чему могу умањити и негативне токсичне ефекте
цитостатика. Наш рад има за циљ да представи најчешће коришћене биљне врсте
у третману канцера у вези са хемијским саставом њихових дрога, тј., доминант-
ним биоактивним компонентама. Такође, биће размотрени и потенцијални ан-
тиканцерогени механизми деловања важнијих биљних секундарних метаболита,
као и њихови ефекти на нпр. апоптозу, инхибицију NF-κB фактора, модулацију
Wnt-бета-катенин сигналних путева, аутофагију, као и позитивне утицаје на ре-
зистентност канцерогених ћелија на различите хемијске агенсе и епигенетичке
одговоре. Иначе је познато да су висока антиоксидативна активност и анти-ин-
фламацијски ефекти биљних дрога повезани са њиховим анти-канцерогеним деј-
ством. Неколико оваквих, у свету добро проучених, као и неколико аутохтоних
врста са нашег подручја, ће бити посебно представљено.",
publisher = "Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine",
journal = "Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina",
title = "Plant bioactive compounds in cancer treatment: myth or hope?",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5293"
}

Dajić-Stevanović, Z., Arsenijević, N., Kolašinac, S., Maksimović-Ivanić, D., Mijatović, S., Tanić, N., Tanić, N.,& Kanjevac, T.. (2022). Plant bioactive compounds in cancer treatment: myth or hope?. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine., 59.
https://hdl.handle.net/21.15107/rcub_ibiss_5293

Dajić-Stevanović Z, Arsenijević N, Kolašinac S, Maksimović-Ivanić D, Mijatović S, Tanić N, Tanić N, Kanjevac T. Plant bioactive compounds in cancer treatment: myth or hope?. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. 2022;:59.
https://hdl.handle.net/21.15107/rcub_ibiss_5293 .

Dajić-Stevanović, Zora, Arsenijević, Nebojša, Kolašinac, Stefan, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Tanić, Nikola, Tanić, Nasta, Kanjevac, Tatjana, "Plant bioactive compounds in cancer treatment: myth or hope?" in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina (2022):59,
https://hdl.handle.net/21.15107/rcub_ibiss_5293 .

TY  - CONF
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Dajić-Stevanović, Zora
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5286
AB  - Eryngium amethystinum is herb from Apiaceae family. It has been used as a food or in traditional medicine for the treatment of various diseases1. In the present work, we have evaluated its cytotoxic effect on a panel of human cancer cells (human breast carcinoma MCF7, human malignant melanoma A375, human colon carcinoma HCT116 and human lung cancer A549). Treatment with Eryngium amethystinum ethanol extract decreased viability of all cancer cell lines in a dose-dependent manner after 72 h. For further investigation of potential mechanism of action A549 cell line was selected. The observed viability decrease was followed by loss of dividing potential after the treatment. Aditionaly, 90% of A549 cells were subjected to programmed cell death ̶ apoptosis which was not followed with caspase activation. In paralel with this, typical apoptotic morphology of treated cells was observed by fluorescent microscopy. Apart from this decreased cell viability was due to triggered autophagic cell death. Taken together, the shown effect of Eryngium amethystinum makes this plant worthwhile for further evaluation in the field of oncology.
PB  - Belgrade: Faculty of Chemistry
C3  - Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
T1  - Antitumor properties of Eryngium amethystinum extract
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5286
ER  - 

@conference{
author = "Jelača, Sanja and Mijatović, Sanja and Dajić-Stevanović, Zora and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Eryngium amethystinum is herb from Apiaceae family. It has been used as a food or in traditional medicine for the treatment of various diseases1. In the present work, we have evaluated its cytotoxic effect on a panel of human cancer cells (human breast carcinoma MCF7, human malignant melanoma A375, human colon carcinoma HCT116 and human lung cancer A549). Treatment with Eryngium amethystinum ethanol extract decreased viability of all cancer cell lines in a dose-dependent manner after 72 h. For further investigation of potential mechanism of action A549 cell line was selected. The observed viability decrease was followed by loss of dividing potential after the treatment. Aditionaly, 90% of A549 cells were subjected to programmed cell death ̶ apoptosis which was not followed with caspase activation. In paralel with this, typical apoptotic morphology of treated cells was observed by fluorescent microscopy. Apart from this decreased cell viability was due to triggered autophagic cell death. Taken together, the shown effect of Eryngium amethystinum makes this plant worthwhile for further evaluation in the field of oncology.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia",
title = "Antitumor properties of Eryngium amethystinum extract",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5286"
}

Jelača, S., Mijatović, S., Dajić-Stevanović, Z., Arsenijević, N.,& Maksimović-Ivanić, D.. (2022). Antitumor properties of Eryngium amethystinum extract. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
Belgrade: Faculty of Chemistry., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5286

Jelača S, Mijatović S, Dajić-Stevanović Z, Arsenijević N, Maksimović-Ivanić D. Antitumor properties of Eryngium amethystinum extract. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. 2022;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5286 .

Jelača, Sanja, Mijatović, Sanja, Dajić-Stevanović, Zora, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Antitumor properties of Eryngium amethystinum extract" in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia (2022):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5286 .

TY  - JOUR
AU  - Bjedov, Ivana
AU  - Obratov-Petković, Dragica
AU  - Rakonjac, Vera
AU  - Skočajić, Dragana
AU  - Bojović, Srđan
AU  - Marković, Milena
AU  - Dajić-Stevanović, Zora
PY  - 2021
UR  - https://journals.pan.pl/dlibra/publication/136700/edition/122183/content
UR  - https://abcbot.pl/2021_63_2-issue.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4860
AB  - Morpho-anatomical characteristics of Vaccinium myrtillus, V. uliginosum and V. vitis-idaea leaves from several sites of the Central Balkans were examined. The aim of this study was to investigate for the first time morpho-anatomical leaf traits of these species in the studied populations and to identify traits that follow a specific trend along the gradients of climate factors. Leaf traits that discriminate Vaccinium species were as follows: depth of the adaxial cuticule (AdC), thickness of the palisade tissue (PT), thickness of the spongy tissue (ST), height of the abaxial epidermal cells (AbE), height of the abaxial cuticule (AbC) and leaf thickness (LT). Populations of V. myrtillus were characterized by the smallest, and populations of V. vitis-idaea by the highest values for AdC, PT, ST, AbE and LT. Additionally, AbC was significantly larger for V. uliginosum in comparison to two other species. On the basis of morpho-anatomical traits, intraspecific variability of the studied species was explored by Principal Component Analysis (PCA), Cluster Analysis (CA) and Analysis of Variance (ANOVA). CA based on 10 morpho-anatomical traits showed that populations of V. myrtillus and V. uliginosum that grew at lower altitudes (characterized by higher mean annual temperature) are more similar to each other. Especially V. myrtillus was responsive to the elevational gradient and exhibited the highest plasticity in morpho-anatomical leaf traits. Populations of V. vitis-idaea had a different pattern of differentiation along the elevational gradient. CA showed that the populations at the lowest and at the highest altitudes were more similar according to the morpho-anatomical leaf traits, meaning that evergreen leaves were more resistant to environmental conditions.
PB  - Warszawa: Polish Academy of Sciences
T2  - Acta Biologica Cracoviensia Series Botanica
T1  - Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans
IS  - 2
VL  - 63
DO  - 10.24425/abcsb.2021.136700
SP  - 7
EP  - 16
ER  - 

@article{
author = "Bjedov, Ivana and Obratov-Petković, Dragica and Rakonjac, Vera and Skočajić, Dragana and Bojović, Srđan and Marković, Milena and Dajić-Stevanović, Zora",
year = "2021",
abstract = "Morpho-anatomical characteristics of Vaccinium myrtillus, V. uliginosum and V. vitis-idaea leaves from several sites of the Central Balkans were examined. The aim of this study was to investigate for the first time morpho-anatomical leaf traits of these species in the studied populations and to identify traits that follow a specific trend along the gradients of climate factors. Leaf traits that discriminate Vaccinium species were as follows: depth of the adaxial cuticule (AdC), thickness of the palisade tissue (PT), thickness of the spongy tissue (ST), height of the abaxial epidermal cells (AbE), height of the abaxial cuticule (AbC) and leaf thickness (LT). Populations of V. myrtillus were characterized by the smallest, and populations of V. vitis-idaea by the highest values for AdC, PT, ST, AbE and LT. Additionally, AbC was significantly larger for V. uliginosum in comparison to two other species. On the basis of morpho-anatomical traits, intraspecific variability of the studied species was explored by Principal Component Analysis (PCA), Cluster Analysis (CA) and Analysis of Variance (ANOVA). CA based on 10 morpho-anatomical traits showed that populations of V. myrtillus and V. uliginosum that grew at lower altitudes (characterized by higher mean annual temperature) are more similar to each other. Especially V. myrtillus was responsive to the elevational gradient and exhibited the highest plasticity in morpho-anatomical leaf traits. Populations of V. vitis-idaea had a different pattern of differentiation along the elevational gradient. CA showed that the populations at the lowest and at the highest altitudes were more similar according to the morpho-anatomical leaf traits, meaning that evergreen leaves were more resistant to environmental conditions.",
publisher = "Warszawa: Polish Academy of Sciences",
journal = "Acta Biologica Cracoviensia Series Botanica",
title = "Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans",
number = "2",
volume = "63",
doi = "10.24425/abcsb.2021.136700",
pages = "7-16"
}

Bjedov, I., Obratov-Petković, D., Rakonjac, V., Skočajić, D., Bojović, S., Marković, M.,& Dajić-Stevanović, Z.. (2021). Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans. in Acta Biologica Cracoviensia Series Botanica
Warszawa: Polish Academy of Sciences., 63(2), 7-16.
https://doi.org/10.24425/abcsb.2021.136700

Bjedov I, Obratov-Petković D, Rakonjac V, Skočajić D, Bojović S, Marković M, Dajić-Stevanović Z. Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans. in Acta Biologica Cracoviensia Series Botanica. 2021;63(2):7-16.
doi:10.24425/abcsb.2021.136700 .

Bjedov, Ivana, Obratov-Petković, Dragica, Rakonjac, Vera, Skočajić, Dragana, Bojović, Srđan, Marković, Milena, Dajić-Stevanović, Zora, "Nontrivial variations of morpho-anatomical leaf traits in natural South-eastern populations of vaccinium species from Central Balkans" in Acta Biologica Cracoviensia Series Botanica, 63, no. 2 (2021):7-16,
https://doi.org/10.24425/abcsb.2021.136700 . .

TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić Stevanović, Zora
AU  - Mijatović, Sanja
AU  - Jovanović, Ivan
AU  - Jovanović, Marina
AU  - Jurišević, Marina
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4426
AB  - Alchemilla vulgaris L. has long history of usage in folk medicine especially against gynecological problems. Ethnomedicinal reports for the territory of Balkan are mentioning its well 
known biological properties against dysmenorrhea, menopausal complaints, infertility, cysts and endometriosis. Based on ethnomedicinal data on female illnesses, the objective of 
our study was to determine the effect of Alchemilla vulgaris L. ethanol extract against breast cancer cells in vitro and in vivo. Our results have showed remarkable viability decrease 
of mouse (4T1) breast cancer cells in dose‐dependent manner after the treatment with Alchemilla vulgaris L. extract. Strong inhibition of cell prolifertion was observed in treated 
cells. In parallel with this, different types of cell death was found. Certain percentage of 4T1 cells was subjected to programmed cell death‐apoptosis which was followed with caspase 
activation and confirmed by fluorescent microscopy observing tipical morphologicas features of apoptosis in treated culture. Estimation of the presence of autophagosomes shown 
that autophagy contributing  to  the cytotoxicity of the  treatment. Also, enhanced production of ROS and intracellular NO after treatment with Alchemilla vulgaris L. was  found. 
In parallel, metastatic potential of this cells is diminished. Apart from the direct effect of A. Vulgaris L. extract on tumor cells, strong potentiation of antitumor immune responce 
manifested dominantly through enhanced accumulation of activated dendritic cells and subsequently CD8+ T cells in spleen and tumor microenvironment. Above briefly described 
mode of action of Alchemilla vulgaris L. against breast cancer cells makes this plant worthwhile for further evaluation in the field of oncology.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model
IS  - Suppl 1
VL  - 51
DO  - 10.1002/eji.202170200
SP  - 351
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić Stevanović, Zora and Mijatović, Sanja and Jovanović, Ivan and Jovanović, Marina and Jurišević, Marina and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2021",
abstract = "Alchemilla vulgaris L. has long history of usage in folk medicine especially against gynecological problems. Ethnomedicinal reports for the territory of Balkan are mentioning its well 
known biological properties against dysmenorrhea, menopausal complaints, infertility, cysts and endometriosis. Based on ethnomedicinal data on female illnesses, the objective of 
our study was to determine the effect of Alchemilla vulgaris L. ethanol extract against breast cancer cells in vitro and in vivo. Our results have showed remarkable viability decrease 
of mouse (4T1) breast cancer cells in dose‐dependent manner after the treatment with Alchemilla vulgaris L. extract. Strong inhibition of cell prolifertion was observed in treated 
cells. In parallel with this, different types of cell death was found. Certain percentage of 4T1 cells was subjected to programmed cell death‐apoptosis which was followed with caspase 
activation and confirmed by fluorescent microscopy observing tipical morphologicas features of apoptosis in treated culture. Estimation of the presence of autophagosomes shown 
that autophagy contributing  to  the cytotoxicity of the  treatment. Also, enhanced production of ROS and intracellular NO after treatment with Alchemilla vulgaris L. was  found. 
In parallel, metastatic potential of this cells is diminished. Apart from the direct effect of A. Vulgaris L. extract on tumor cells, strong potentiation of antitumor immune responce 
manifested dominantly through enhanced accumulation of activated dendritic cells and subsequently CD8+ T cells in spleen and tumor microenvironment. Above briefly described 
mode of action of Alchemilla vulgaris L. against breast cancer cells makes this plant worthwhile for further evaluation in the field of oncology.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model",
number = "Suppl 1",
volume = "51",
doi = "10.1002/eji.202170200",
pages = "351"
}

Jelača, S., Drača, D., Dajić Stevanović, Z., Mijatović, S., Jovanović, I., Jovanović, M., Jurišević, M., Arsenijević, N.,& Maksimović-Ivanić, D.. (2021). Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 51(Suppl 1), 351.
https://doi.org/10.1002/eji.202170200

Jelača S, Drača D, Dajić Stevanović Z, Mijatović S, Jovanović I, Jovanović M, Jurišević M, Arsenijević N, Maksimović-Ivanić D. Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;51(Suppl 1):351.
doi:10.1002/eji.202170200 .

Jelača, Sanja, Drača, Dijana, Dajić Stevanović, Zora, Mijatović, Sanja, Jovanović, Ivan, Jovanović, Marina, Jurišević, Marina, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting, 51, no. Suppl 1 (2021):351,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Zdunić, Gordana
AU  - Dajić-Stevanović, Zora
AU  - Gođevac, Dejan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3613
AB  - This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.
T2  - Journal of the Serbian Chemical Society
T1  - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
IS  - 12
VL  - 84
DO  - 10.2298/jsc190513106s
SP  - 1355
EP  - 1365
ER  - 

@article{
author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Gođevac, Dejan",
year = "2019",
abstract = "This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.",
journal = "Journal of the Serbian Chemical Society",
title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil",
number = "12",
volume = "84",
doi = "10.2298/jsc190513106s",
pages = "1355-1365"
}

Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Gođevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society, 84(12), 1355-1365.
https://doi.org/10.2298/jsc190513106s

Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Gođevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society. 2019;84(12):1355-1365.
doi:10.2298/jsc190513106s .

Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Gođevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in Journal of the Serbian Chemical Society, 84, no. 12 (2019):1355-1365,
https://doi.org/10.2298/jsc190513106s . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Gođevac, Dejan
AU  - Tešević, Vele
AU  - Dajić-Stevanović, Zora
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Novaković, Miroslav
PY  - 2019
UR  - http://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3397
AB  - A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.
PB  - American Chemical Society and American Society of Pharmacognosy
T2  - Journal of Natural Products
T1  - Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.
IS  - 6
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00970
SP  - 1487
EP  - 1495
ER  - 

@article{
author = "Stanković, Jovana and Gođevac, Dejan and Tešević, Vele and Dajić-Stevanović, Zora and Ćirić, Ana and Soković, Marina and Novaković, Miroslav",
year = "2019",
abstract = "A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.",
publisher = "American Chemical Society and American Society of Pharmacognosy",
journal = "Journal of Natural Products",
title = "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.",
number = "6",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00970",
pages = "1487-1495"
}

Stanković, J., Gođevac, D., Tešević, V., Dajić-Stevanović, Z., Ćirić, A., Soković, M.,& Novaković, M.. (2019). Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products
American Chemical Society and American Society of Pharmacognosy., 82(6), 1487-1495.
https://doi.org/10.1021/acs.jnatprod.8b00970

Stanković J, Gođevac D, Tešević V, Dajić-Stevanović Z, Ćirić A, Soković M, Novaković M. Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products. 2019;82(6):1487-1495.
doi:10.1021/acs.jnatprod.8b00970 .

Stanković, Jovana, Gođevac, Dejan, Tešević, Vele, Dajić-Stevanović, Zora, Ćirić, Ana, Soković, Marina, Novaković, Miroslav, "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa." in Journal of Natural Products, 82, no. 6 (2019):1487-1495,
https://doi.org/10.1021/acs.jnatprod.8b00970 . .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Jarić, Snežana
AU  - Dajić-Stevanović, Zora
AU  - Duletić-Laušević, Sonja
PY  - 2019
UR  - http://link.springer.com/10.1007/s10722-018-00734-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3312
AB  - This work represents an ethnobotanical and ethnomedicinal study focused on recording autochthonous pear varieties of the Polimlje region in southwest Serbia. The purpose of the study was to highlight genetic resources of pear varieties in situ and emphasize aspects of ethnodiversity and traditional knowledge. A special feature of the research area is the great diversity of indigenous varieties of pear, which have been cultivated for hundreds of years. Forty-six respondents from 25 to 78 years of age residing in 23 villages were interviewed, and 26 autochthonous pear varieties were recorded in the field. Among them 11.5% are widely available, 26.9% are rare, and 61.5% are very rare. For ethnomedicinal purposes, pear is used as a remedy against hypertension, diabetes, high cholesterol, and constipation; for reduction of body mass; as a uroseptic and/or an antirheumatic; and for cancer prevention. Pears are consumed as fresh fruit and in numerous food products. Depopulation of the investigated rural area is directly responsible for depletion of the gene pool of pear varieties and for loss of traditional knowledge. Preservation can be achieved by in situ methods such as raising awareness of the local inhabitants and ensuring support of the State.
T2  - Genetic Resources and Crop Evolution
T1  - Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)
IS  - 3
VL  - 66
DO  - 10.1007/s10722-018-00734-w
SP  - 589
EP  - 609
ER  - 

@article{
author = "Savić, Aleksandra and Jarić, Snežana and Dajić-Stevanović, Zora and Duletić-Laušević, Sonja",
year = "2019",
abstract = "This work represents an ethnobotanical and ethnomedicinal study focused on recording autochthonous pear varieties of the Polimlje region in southwest Serbia. The purpose of the study was to highlight genetic resources of pear varieties in situ and emphasize aspects of ethnodiversity and traditional knowledge. A special feature of the research area is the great diversity of indigenous varieties of pear, which have been cultivated for hundreds of years. Forty-six respondents from 25 to 78 years of age residing in 23 villages were interviewed, and 26 autochthonous pear varieties were recorded in the field. Among them 11.5% are widely available, 26.9% are rare, and 61.5% are very rare. For ethnomedicinal purposes, pear is used as a remedy against hypertension, diabetes, high cholesterol, and constipation; for reduction of body mass; as a uroseptic and/or an antirheumatic; and for cancer prevention. Pears are consumed as fresh fruit and in numerous food products. Depopulation of the investigated rural area is directly responsible for depletion of the gene pool of pear varieties and for loss of traditional knowledge. Preservation can be achieved by in situ methods such as raising awareness of the local inhabitants and ensuring support of the State.",
journal = "Genetic Resources and Crop Evolution",
title = "Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)",
number = "3",
volume = "66",
doi = "10.1007/s10722-018-00734-w",
pages = "589-609"
}

Savić, A., Jarić, S., Dajić-Stevanović, Z.,& Duletić-Laušević, S.. (2019). Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje). in Genetic Resources and Crop Evolution, 66(3), 589-609.
https://doi.org/10.1007/s10722-018-00734-w

Savić A, Jarić S, Dajić-Stevanović Z, Duletić-Laušević S. Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje). in Genetic Resources and Crop Evolution. 2019;66(3):589-609.
doi:10.1007/s10722-018-00734-w .

Savić, Aleksandra, Jarić, Snežana, Dajić-Stevanović, Zora, Duletić-Laušević, Sonja, "Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje)" in Genetic Resources and Crop Evolution, 66, no. 3 (2019):589-609,
https://doi.org/10.1007/s10722-018-00734-w . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Pećinar, Ilinka
AU  - Brkušanin, Miloš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Dajić Stevanović, Zora
AU  - Schulz, Hartwig
AU  - Mišić, Danijela
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0926669018302413?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3022
AB  - Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.
T2  - Industrial Crops and Products
T1  - Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants
VL  - 117
DO  - 10.1016/J.INDCROP.2018.03.019
SP  - 347
EP  - 358
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Pećinar, Ilinka and Brkušanin, Miloš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Dajić Stevanović, Zora and Schulz, Hartwig and Mišić, Danijela",
year = "2018",
abstract = "Nepetalactones are accumulated as the main secondary metabolites in the majority of Nepeta species; however, their biosynthetic pathway remains to a great extent unknown, as well as their relationship to the overall physiology of these plants. Nepetalactones most certainly arise through general iridoid biosynthetic pathway leading to nepetalactol, a common precursor of all plant-derived iridoids that is formed in a reaction catalysed by iridoid synthase (IS, EC 1.3.1.99). In this paper, we aimed to localize the sites of nepetalactone biosynthesis and accumulation by comparatively analysing the nepetalactones content and the expression of putative iridoid synthase (NrIS) in leaves of Nepeta rtanjensis Diklić & Milojević, a trans,cis-nepetalactone and dehydronepetalactone rich species. Another aim was to give an insight into the developmental regulation of nepetalactone biosynthesis in leaves of cultivated N. rtanjensis plants. Knowledge on the spatial-, and developmental- regulation of nepetalactone metabolism could greatly benefit potential growers by enabling them to adequately plan and manage plant cultivation, harvesting, and handling and thus increase yield of bioactive compounds. The expression profile of putative NrIS1 is highly correlated with trans,cis-nepetalactone and dehydronepetalactone content/ratio in leaves of different developmental stages. The results strongly indicate that developmental regulation of nepetalactone biosynthesis in leaves resides largely at the level of gene expression, and suggest an important role of NrIS1. Young leaves exhibited the most intensive nepetalactone biosynthesis and accumulation, but also its conversion to dehydronepetalactone. Thus, the pattern of NL accumulation in leaves can be explained by both the rate of its biosynthesis and metabolic degradation. The gene expression patterns of NrIS1 and NrIS2 transcripts in trichomes and abraded leaves revealed trichomes as the main site of their activity. UHPLC/qqqMS profiling of nepetalactones in trichomes and abraded leaves, complemented with Raman spectroscopy and dichloromethane dipping experiment, supported glandular trichome-specific localization of nepetalactone biosynthesis and accumulation.",
journal = "Industrial Crops and Products",
title = "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants",
volume = "117",
doi = "10.1016/J.INDCROP.2018.03.019",
pages = "347-358"
}

Aničić, N., Matekalo, D., Skorić, M., Pećinar, I., Brkušanin, M., Nestorović Živković, J., Dmitrović, S., Dajić Stevanović, Z., Schulz, H.,& Mišić, D.. (2018). Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products, 117, 347-358.
https://doi.org/10.1016/J.INDCROP.2018.03.019

Aničić N, Matekalo D, Skorić M, Pećinar I, Brkušanin M, Nestorović Živković J, Dmitrović S, Dajić Stevanović Z, Schulz H, Mišić D. Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants. in Industrial Crops and Products. 2018;117:347-358.
doi:10.1016/J.INDCROP.2018.03.019 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Pećinar, Ilinka, Brkušanin, Miloš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Dajić Stevanović, Zora, Schulz, Hartwig, Mišić, Danijela, "Trichome-specific and developmentally regulated biosynthesis of nepetalactones in leaves of cultivated Nepeta rtanjensis plants" in Industrial Crops and Products, 117 (2018):347-358,
https://doi.org/10.1016/J.INDCROP.2018.03.019 . .

TY  - CONF
AU  - Savić, Aleksandra
AU  - Jarić, Snežana
AU  - Dajić Stevanović, Zora
AU  - Duletić-Laušević, Sonja
PY  - 2018
UR  - https://botanicaserbica.bio.bg.ac.rs/2018_42_2_1.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5421
AB  - Th is study   documents the ethnobotanical and ethnomedicinal knowledge of the local inhabitants in the region Polimlje in Southwest Serbia, focused on recording rare autochthonous pear varieties (Pyrus communis). A special feature of Polimlje, as an unique multiethnic complex of natural and cultural heritage, is the great diversity of indigenous varieties of pears and breeding tradition in the households for hundreds of years. Objectives of the study were: to establish the diversity of indigenous pear varieties in order to preserve the unique P. communis gene fond characteristic to this region, and to explore their use in ethnomedicine and traditional food production. Th e opened and semi-structured interviews were done with 40 people aged between 30-80 years (29 men and 11 women), during June - September 2015, on the territory of 23 villages. Th e results of this study indicate the presence of 23 autochtonous pear varieties: 7 are determined as rare (Vidovača, Ječmenjača, Ilinjača, Lubeničarka, Medunak, Zimnjača, Takiša) and 16 as extremely rare (Jagodarka, Mirisavka, Sijerak, Turundžija, Stambolka, Čađavica, Okruglica, Mesnjača, Jarac, Bazva, Tepavac, Lončara, Kantaruša, Ovčara, Turšijara, Budaljača). A lot of varieties remain in the old and abandoned homes and the rugged terrain. For medicinal purpose pear is used (fresh or dried fruit, juice or compote) as antihypertensive remedy (65.2%), antidiabetic or anticholesterol (63%), anticonstipation remedy (54.3%), as well as for body mass reduction (65.2%). Tea made from the pear skin is used for urolithiasis - stone and sand in the kidney (36.9%), pear tea and fresh fruit juice are applied as antirheumatics (26%) and for cancer prevention (10.87%). Pears are consumed as fresh fruit, in pies and cakes, or they are processed in a compote, juice, jam or marmalade, baby food, jelly, syrup, dried fruit, pear brandy and other traditional products (sita, vodnjika). Depopulation of investigated rural area is directly related to disappearance of pear varieties gene pool, thereby losing traditional knowledge. Preservation can be achieved with in situ methods, by raising the awareness of the inhabitants and through the support of the State.
PB  - Belgrade: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade
C3  - Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1).
T1  - Diversity of rare indigenous pear varieties (Pyrus communis L.) In the region Polimlje, (southwest Serbia) and their use in ethnomedicine and nutrition
SP  - 196
EP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5421
ER  - 

@conference{
author = "Savić, Aleksandra and Jarić, Snežana and Dajić Stevanović, Zora and Duletić-Laušević, Sonja",
year = "2018",
abstract = "Th is study   documents the ethnobotanical and ethnomedicinal knowledge of the local inhabitants in the region Polimlje in Southwest Serbia, focused on recording rare autochthonous pear varieties (Pyrus communis). A special feature of Polimlje, as an unique multiethnic complex of natural and cultural heritage, is the great diversity of indigenous varieties of pears and breeding tradition in the households for hundreds of years. Objectives of the study were: to establish the diversity of indigenous pear varieties in order to preserve the unique P. communis gene fond characteristic to this region, and to explore their use in ethnomedicine and traditional food production. Th e opened and semi-structured interviews were done with 40 people aged between 30-80 years (29 men and 11 women), during June - September 2015, on the territory of 23 villages. Th e results of this study indicate the presence of 23 autochtonous pear varieties: 7 are determined as rare (Vidovača, Ječmenjača, Ilinjača, Lubeničarka, Medunak, Zimnjača, Takiša) and 16 as extremely rare (Jagodarka, Mirisavka, Sijerak, Turundžija, Stambolka, Čađavica, Okruglica, Mesnjača, Jarac, Bazva, Tepavac, Lončara, Kantaruša, Ovčara, Turšijara, Budaljača). A lot of varieties remain in the old and abandoned homes and the rugged terrain. For medicinal purpose pear is used (fresh or dried fruit, juice or compote) as antihypertensive remedy (65.2%), antidiabetic or anticholesterol (63%), anticonstipation remedy (54.3%), as well as for body mass reduction (65.2%). Tea made from the pear skin is used for urolithiasis - stone and sand in the kidney (36.9%), pear tea and fresh fruit juice are applied as antirheumatics (26%) and for cancer prevention (10.87%). Pears are consumed as fresh fruit, in pies and cakes, or they are processed in a compote, juice, jam or marmalade, baby food, jelly, syrup, dried fruit, pear brandy and other traditional products (sita, vodnjika). Depopulation of investigated rural area is directly related to disappearance of pear varieties gene pool, thereby losing traditional knowledge. Preservation can be achieved with in situ methods, by raising the awareness of the inhabitants and through the support of the State.",
publisher = "Belgrade: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade",
journal = "Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1).",
title = "Diversity of rare indigenous pear varieties (Pyrus communis L.) In the region Polimlje, (southwest Serbia) and their use in ethnomedicine and nutrition",
pages = "196-197",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5421"
}

Savić, A., Jarić, S., Dajić Stevanović, Z.,& Duletić-Laušević, S.. (2018). Diversity of rare indigenous pear varieties (Pyrus communis L.) In the region Polimlje, (southwest Serbia) and their use in ethnomedicine and nutrition. in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1).
Belgrade: Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology - University of Belgrade., 196-197.
https://hdl.handle.net/21.15107/rcub_ibiss_5421

Savić A, Jarić S, Dajić Stevanović Z, Duletić-Laušević S. Diversity of rare indigenous pear varieties (Pyrus communis L.) In the region Polimlje, (southwest Serbia) and their use in ethnomedicine and nutrition. in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1).. 2018;:196-197.
https://hdl.handle.net/21.15107/rcub_ibiss_5421 .

Savić, Aleksandra, Jarić, Snežana, Dajić Stevanović, Zora, Duletić-Laušević, Sonja, "Diversity of rare indigenous pear varieties (Pyrus communis L.) In the region Polimlje, (southwest Serbia) and their use in ethnomedicine and nutrition" in Book of abstracts: 7th Balkan Botanical Congress; 2018 Sep 10-14; Novi Sad, Serbia. (Botanica Serbica; Vol. 42; Suppl. 1). (2018):196-197,
https://hdl.handle.net/21.15107/rcub_ibiss_5421 .

TY  - JOUR
AU  - Mačukanović-Jocić, Marina
AU  - Dajić Stevanović, Zora
AU  - Jarić, Snežana
AU  - Đurđević, Lola
PY  - 2008
UR  - https://www.tandfonline.com/doi/abs/10.1080/00218839.2008.11101431
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6096
PB  - Oxon: Taylor and Francis
T2  - Journal of Apicultural Research and Bee World
T1  - Nectar secretion in basil (Ocimum basilicum L.) grown in different soil conditions
IS  - 1
VL  - 47
DO  - 10.1080/00218839.2008.11101431
SP  - 89
EP  - 90
ER  - 

@article{
author = "Mačukanović-Jocić, Marina and Dajić Stevanović, Zora and Jarić, Snežana and Đurđević, Lola",
year = "2008",
publisher = "Oxon: Taylor and Francis",
journal = "Journal of Apicultural Research and Bee World",
title = "Nectar secretion in basil (Ocimum basilicum L.) grown in different soil conditions",
number = "1",
volume = "47",
doi = "10.1080/00218839.2008.11101431",
pages = "89-90"
}

Mačukanović-Jocić, M., Dajić Stevanović, Z., Jarić, S.,& Đurđević, L.. (2008). Nectar secretion in basil (Ocimum basilicum L.) grown in different soil conditions. in Journal of Apicultural Research and Bee World
Oxon: Taylor and Francis., 47(1), 89-90.
https://doi.org/10.1080/00218839.2008.11101431

Mačukanović-Jocić M, Dajić Stevanović Z, Jarić S, Đurđević L. Nectar secretion in basil (Ocimum basilicum L.) grown in different soil conditions. in Journal of Apicultural Research and Bee World. 2008;47(1):89-90.
doi:10.1080/00218839.2008.11101431 .

Mačukanović-Jocić, Marina, Dajić Stevanović, Zora, Jarić, Snežana, Đurđević, Lola, "Nectar secretion in basil (Ocimum basilicum L.) grown in different soil conditions" in Journal of Apicultural Research and Bee World, 47, no. 1 (2008):89-90,
https://doi.org/10.1080/00218839.2008.11101431 . .

TY  - JOUR
AU  - Vrbničanin, Sava P.
AU  - Karadžić, Branko
AU  - Dajić-Stevanović, Zora
PY  - 2004
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/42
AB  - Invasive alien species are taxa that are deliberately or incidentally dispersed from their natural habitats and introduced into new ecosystems, where they show ability to out compete native species and occupy new habitats. The ecology of invasion by animals and plants has been analyzed in numerous articles. As more powerful competitors, introduced taxa may threaten the existence of native (in some cases rare or endemic) species. Moreover, introduction of new species may cause an introduction of organisms that are pathogenic to natives, but not to the introduced species. In such cases, the pathogenic disease may cause significant reduction in the biodiversity of native habitats. The problem of introduced species has been emphasized in recent times because of the globalization of markets, increased trade, travel and tourism. Considering such unfavorable trends, invasive alien species are recognized as one of the major threats to biodiversity (Convention on Biological Diversity). We analyzed effects of invasive alien species on agricultural communities, and surveyed such species in agricultural, ruderal and aquatic ecosystems in Serbia. We analyzed strategies that can be employed to prevent invasive spread of alien species.
T2  - Acta biologica iugoslavica - serija G: Acta herbologica
T1  - Adventivne i invazivne korovske vrste na području Srbije
T1  - Adventive and invasive weed species in Serbia
IS  - 1
VL  - 13
SP  - 1
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_42
ER  - 

@article{
author = "Vrbničanin, Sava P. and Karadžić, Branko and Dajić-Stevanović, Zora",
year = "2004, 2004",
abstract = "Invasive alien species are taxa that are deliberately or incidentally dispersed from their natural habitats and introduced into new ecosystems, where they show ability to out compete native species and occupy new habitats. The ecology of invasion by animals and plants has been analyzed in numerous articles. As more powerful competitors, introduced taxa may threaten the existence of native (in some cases rare or endemic) species. Moreover, introduction of new species may cause an introduction of organisms that are pathogenic to natives, but not to the introduced species. In such cases, the pathogenic disease may cause significant reduction in the biodiversity of native habitats. The problem of introduced species has been emphasized in recent times because of the globalization of markets, increased trade, travel and tourism. Considering such unfavorable trends, invasive alien species are recognized as one of the major threats to biodiversity (Convention on Biological Diversity). We analyzed effects of invasive alien species on agricultural communities, and surveyed such species in agricultural, ruderal and aquatic ecosystems in Serbia. We analyzed strategies that can be employed to prevent invasive spread of alien species.",
journal = "Acta biologica iugoslavica - serija G: Acta herbologica",
title = "Adventivne i invazivne korovske vrste na području Srbije, Adventive and invasive weed species in Serbia",
number = "1",
volume = "13",
pages = "1-12",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_42"
}

Vrbničanin, S. P., Karadžić, B.,& Dajić-Stevanović, Z.. (2004). Adventivne i invazivne korovske vrste na području Srbije. in Acta biologica iugoslavica - serija G: Acta herbologica, 13(1), 1-12.
https://hdl.handle.net/21.15107/rcub_ibiss_42

Vrbničanin SP, Karadžić B, Dajić-Stevanović Z. Adventivne i invazivne korovske vrste na području Srbije. in Acta biologica iugoslavica - serija G: Acta herbologica. 2004;13(1):1-12.
https://hdl.handle.net/21.15107/rcub_ibiss_42 .

Vrbničanin, Sava P., Karadžić, Branko, Dajić-Stevanović, Zora, "Adventivne i invazivne korovske vrste na području Srbije" in Acta biologica iugoslavica - serija G: Acta herbologica, 13, no. 1 (2004):1-12,
https://hdl.handle.net/21.15107/rcub_ibiss_42 .