TY  - JOUR
AU  - Jelača, Sanja
AU  - Jovanović, Ivan
AU  - Bovan, Dijana
AU  - Jovanović, Marina Z.
AU  - Jurišević, Milena M.
AU  - Dunđerović, Duško
AU  - Dajić-Stevanović, Zora
AU  - Arsenijević, Nebojša
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6575
AB  - Ethnomedicinal records have long mentioned the historical usage of Alchemilla vulgaris L. in folk medicine, particularly for the treatment of gynecological issues. Building on this ethnomedicinal knowledge regarding female illnesses, the aim of this research was to evaluate the impact of ethanolic extract of A. vulgaris on mouse breast cancer cells (4T1) in vitro and in vivo, in addition to its effect on the immune compartment in the tumor microenvironment. Behind viability decrease of 4T1 cells induced by treatment with A. vulgaris extract was strong inhibition of cell proliferation accompanied by caspase-dependent apoptosis and autophagic cell death. Observed changes in 4T1 cell culture after treatment were well orchestrated and led to a reduction in metastatic potential through weakened adhesion, invasion, migration, and colony-forming abilities in vitro. Enhanced intracellular production of reactive oxygen and nitrogen species promoted by the treatment might interfere with all the observed effects. Apart from the direct effect on tumor cells, the A. vulgaris extract significantly reduced tumor growth in the solid orthotropic mammary carcinoma model through restitution of efficient local and systemic immune response reflected in enhanced antigen-presenting potential of dendritic cells (DCs) as well as the extent and activity of effector T cells.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response
IS  - 3
VL  - 17
DO  - 10.3390/ph17030286
SP  - 286
ER  - 

@article{
author = "Jelača, Sanja and Jovanović, Ivan and Bovan, Dijana and Jovanović, Marina Z. and Jurišević, Milena M. and Dunđerović, Duško and Dajić-Stevanović, Zora and Arsenijević, Nebojša and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2024",
abstract = "Ethnomedicinal records have long mentioned the historical usage of Alchemilla vulgaris L. in folk medicine, particularly for the treatment of gynecological issues. Building on this ethnomedicinal knowledge regarding female illnesses, the aim of this research was to evaluate the impact of ethanolic extract of A. vulgaris on mouse breast cancer cells (4T1) in vitro and in vivo, in addition to its effect on the immune compartment in the tumor microenvironment. Behind viability decrease of 4T1 cells induced by treatment with A. vulgaris extract was strong inhibition of cell proliferation accompanied by caspase-dependent apoptosis and autophagic cell death. Observed changes in 4T1 cell culture after treatment were well orchestrated and led to a reduction in metastatic potential through weakened adhesion, invasion, migration, and colony-forming abilities in vitro. Enhanced intracellular production of reactive oxygen and nitrogen species promoted by the treatment might interfere with all the observed effects. Apart from the direct effect on tumor cells, the A. vulgaris extract significantly reduced tumor growth in the solid orthotropic mammary carcinoma model through restitution of efficient local and systemic immune response reflected in enhanced antigen-presenting potential of dendritic cells (DCs) as well as the extent and activity of effector T cells.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response",
number = "3",
volume = "17",
doi = "10.3390/ph17030286",
pages = "286"
}

Jelača, S., Jovanović, I., Bovan, D., Jovanović, M. Z., Jurišević, M. M., Dunđerović, D., Dajić-Stevanović, Z., Arsenijević, N., Mijatović, S.,& Maksimović-Ivanić, D.. (2024). Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response. in Pharmaceuticals
Basel: MDPI., 17(3), 286.
https://doi.org/10.3390/ph17030286

Jelača S, Jovanović I, Bovan D, Jovanović MZ, Jurišević MM, Dunđerović D, Dajić-Stevanović Z, Arsenijević N, Mijatović S, Maksimović-Ivanić D. Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response. in Pharmaceuticals. 2024;17(3):286.
doi:10.3390/ph17030286 .

Jelača, Sanja, Jovanović, Ivan, Bovan, Dijana, Jovanović, Marina Z., Jurišević, Milena M., Dunđerović, Duško, Dajić-Stevanović, Zora, Arsenijević, Nebojša, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Dual Role of Alchemilla vulgaris L. Extract in Breast Cancer Regression: Reestablishment of Effective Immune Response" in Pharmaceuticals, 17, no. 3 (2024):286,
https://doi.org/10.3390/ph17030286 . .

TY  - CONF
AU  - Krajnović, Tamara
AU  - Bovan, Dijana
AU  - Vuković, Nenad L.
AU  - Vukić, Milena D.
AU  - Mijatović, Sanja
AU  - Tanić, Nikola
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6267
AB  - Background: Shikonins are naturally occurring naphthoquinones found in the roots of several genera of the Boraginaceae family, widely known for their antiinflammatory, antioxidant, antimicrobial, and anticancer properties. This study aimed to investigate the antitumor potential of six shikonins isolated from the roots of Onosma visianii against highly aggressive rat glioma cell line C6 and to explore the mechanisms involved. Material and Methods: Cell viability was estimated by MTT and CV assays. Cell death, proliferation rate, and caspase activity were assessed using flow cytometric analysis of annexin V-FITC/propidium iodide, CFSE, and ApoStat staining, respectively. Fluorescent microscopy of propidium iodide-stained cells was employed for the detection of nuclear morphology. To evaluate the viability of detached cells, an acidic phosphatase assay was used. The cells’ property to adhere was assessed by cell adhesion assay while western blot was engaged to measure the expression of relevant proteins responsible for the observed phenomenon. Results and Conclusions: All examined shikonins dose-dependently decreased the viability of C6 cells, with compounds 5 and 6 being the most potent ones. Compound 5 had a more profound effect on the proliferation rate of C6 cells than compound 6, resulting in almost 70% of inhibition of cell division. Additionally, compound 5, but not compound 6 generated a significant number of early and late apoptotic cells in treated cultures as detected by flow cytometry. In collision with this, typical morphological signs of apoptotic cells were not observed, and fluorescent microscopy revealed only the presence of enlarged nuclei. This paradox was resolved by the discovery of massive detached cell presence, indicating that glioma cells underwent anoikis, a cell attachment-dependent programmed cell death, in response to treatment with both agents. Decreased ability of C6 cells to adhere to the extracellular matrix and compromised integrin signaling was further confirmed by adhesion assay and western blot, respectively. Interestingly, while compound 5 triggered caspase-mediated anoikis, compound 6 realized anoikis in a caspase-independent manner and under sustained ERK1/2 activation, indicating the deviation from standard proanoikis signaling. This study represents the first proof of shikonin derivatives’ strong anticancer potential realized through the induction of anoikis of highly proliferative and invasive malignant glioma cells.
PB  - Belgrade: Serbian Association for Cancer Research
C3  - Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
T1  - Anoikis as a novel mode of shikonin derivatives anticancer action on C6 glioma cells
SP  - 98
EP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6267
ER  - 

@conference{
author = "Krajnović, Tamara and Bovan, Dijana and Vuković, Nenad L. and Vukić, Milena D. and Mijatović, Sanja and Tanić, Nikola and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2023",
abstract = "Background: Shikonins are naturally occurring naphthoquinones found in the roots of several genera of the Boraginaceae family, widely known for their antiinflammatory, antioxidant, antimicrobial, and anticancer properties. This study aimed to investigate the antitumor potential of six shikonins isolated from the roots of Onosma visianii against highly aggressive rat glioma cell line C6 and to explore the mechanisms involved. Material and Methods: Cell viability was estimated by MTT and CV assays. Cell death, proliferation rate, and caspase activity were assessed using flow cytometric analysis of annexin V-FITC/propidium iodide, CFSE, and ApoStat staining, respectively. Fluorescent microscopy of propidium iodide-stained cells was employed for the detection of nuclear morphology. To evaluate the viability of detached cells, an acidic phosphatase assay was used. The cells’ property to adhere was assessed by cell adhesion assay while western blot was engaged to measure the expression of relevant proteins responsible for the observed phenomenon. Results and Conclusions: All examined shikonins dose-dependently decreased the viability of C6 cells, with compounds 5 and 6 being the most potent ones. Compound 5 had a more profound effect on the proliferation rate of C6 cells than compound 6, resulting in almost 70% of inhibition of cell division. Additionally, compound 5, but not compound 6 generated a significant number of early and late apoptotic cells in treated cultures as detected by flow cytometry. In collision with this, typical morphological signs of apoptotic cells were not observed, and fluorescent microscopy revealed only the presence of enlarged nuclei. This paradox was resolved by the discovery of massive detached cell presence, indicating that glioma cells underwent anoikis, a cell attachment-dependent programmed cell death, in response to treatment with both agents. Decreased ability of C6 cells to adhere to the extracellular matrix and compromised integrin signaling was further confirmed by adhesion assay and western blot, respectively. Interestingly, while compound 5 triggered caspase-mediated anoikis, compound 6 realized anoikis in a caspase-independent manner and under sustained ERK1/2 activation, indicating the deviation from standard proanoikis signaling. This study represents the first proof of shikonin derivatives’ strong anticancer potential realized through the induction of anoikis of highly proliferative and invasive malignant glioma cells.",
publisher = "Belgrade: Serbian Association for Cancer Research",
journal = "Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia",
title = "Anoikis as a novel mode of shikonin derivatives anticancer action on C6 glioma cells",
pages = "98-99",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6267"
}

Krajnović, T., Bovan, D., Vuković, N. L., Vukić, M. D., Mijatović, S., Tanić, N., Arsenijević, N.,& Maksimović-Ivanić, D.. (2023). Anoikis as a novel mode of shikonin derivatives anticancer action on C6 glioma cells. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
Belgrade: Serbian Association for Cancer Research., 98-99.
https://hdl.handle.net/21.15107/rcub_ibiss_6267

Krajnović T, Bovan D, Vuković NL, Vukić MD, Mijatović S, Tanić N, Arsenijević N, Maksimović-Ivanić D. Anoikis as a novel mode of shikonin derivatives anticancer action on C6 glioma cells. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia. 2023;:98-99.
https://hdl.handle.net/21.15107/rcub_ibiss_6267 .

Krajnović, Tamara, Bovan, Dijana, Vuković, Nenad L., Vukić, Milena D., Mijatović, Sanja, Tanić, Nikola, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Anoikis as a novel mode of shikonin derivatives anticancer action on C6 glioma cells" in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia (2023):98-99,
https://hdl.handle.net/21.15107/rcub_ibiss_6267 .

TY  - CONF
AU  - Krajnović, Tamara
AU  - Bovan, Dijana
AU  - Vuković, Nenad L.
AU  - Vukić, Milena D.
AU  - Mihajlović, Sanja
AU  - Tanić, Nikola
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6237
AB  - Shikonin is a naphthoquinone found in the roots of plants of the Boraginaceae family and
is widely known for its numerous biological activities, including anticancer. In this study,
the antitumor mode of action of shikonin derivatives isolated from the roots of Onosma
visianii was investigated in mouse melanoma cell line B16. MTT and CV assays showed
that six examined shikonins decreased B16 cell viability in a dose-dependent manner, with
compounds 5 and 6 exhibiting the highest cytotoxic activity. This effect correlated with
caspase-mediated apoptosis, which was detected by flow cytometry and fluorescence
microscopy. In addition, CFSE staining revealed a strong blockage of cell division in
response to treatment, with a more profound effect of compound 6. The altered cell
morphology together with the loss of dividing potential upon exposure to both shikonins
implied reprogramming of the B16 cell phenotype. The absence of melanogenesis
enhancement coupled with an elevated level of myelin basic protein in response to
treatment with both tested agents suggested that the cells transdifferentiated into a
Schwann-like phenotype, with possible involvement of the autophagic process in this
conversion. Differentiation of malignant cells has become favourable in cancer treatment,
bearing in mind the phenomenon of apoptosis-induced proliferation. Hence, the specific
antitumor mode of action of shikonin derivatives on melanoma in vitro shown here
provides a good platform for new investigations of these promising natural compounds.
PB  - Belgrade: Faculty of Chemistry
C3  - Biochemistry in Biotechnology": Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Shikonin derivatives trigger phenotype reprogramming of B16 mouse melanoma cells
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6237
ER  - 

@conference{
author = "Krajnović, Tamara and Bovan, Dijana and Vuković, Nenad L. and Vukić, Milena D. and Mihajlović, Sanja and Tanić, Nikola and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2023",
abstract = "Shikonin is a naphthoquinone found in the roots of plants of the Boraginaceae family and
is widely known for its numerous biological activities, including anticancer. In this study,
the antitumor mode of action of shikonin derivatives isolated from the roots of Onosma
visianii was investigated in mouse melanoma cell line B16. MTT and CV assays showed
that six examined shikonins decreased B16 cell viability in a dose-dependent manner, with
compounds 5 and 6 exhibiting the highest cytotoxic activity. This effect correlated with
caspase-mediated apoptosis, which was detected by flow cytometry and fluorescence
microscopy. In addition, CFSE staining revealed a strong blockage of cell division in
response to treatment, with a more profound effect of compound 6. The altered cell
morphology together with the loss of dividing potential upon exposure to both shikonins
implied reprogramming of the B16 cell phenotype. The absence of melanogenesis
enhancement coupled with an elevated level of myelin basic protein in response to
treatment with both tested agents suggested that the cells transdifferentiated into a
Schwann-like phenotype, with possible involvement of the autophagic process in this
conversion. Differentiation of malignant cells has become favourable in cancer treatment,
bearing in mind the phenomenon of apoptosis-induced proliferation. Hence, the specific
antitumor mode of action of shikonin derivatives on melanoma in vitro shown here
provides a good platform for new investigations of these promising natural compounds.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology": Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Shikonin derivatives trigger phenotype reprogramming of B16 mouse melanoma cells",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6237"
}

Krajnović, T., Bovan, D., Vuković, N. L., Vukić, M. D., Mihajlović, S., Tanić, N., Arsenijević, N.,& Maksimović-Ivanić, D.. (2023). Shikonin derivatives trigger phenotype reprogramming of B16 mouse melanoma cells. in Biochemistry in Biotechnology": Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_6237

Krajnović T, Bovan D, Vuković NL, Vukić MD, Mihajlović S, Tanić N, Arsenijević N, Maksimović-Ivanić D. Shikonin derivatives trigger phenotype reprogramming of B16 mouse melanoma cells. in Biochemistry in Biotechnology": Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_6237 .

Krajnović, Tamara, Bovan, Dijana, Vuković, Nenad L., Vukić, Milena D., Mihajlović, Sanja, Tanić, Nikola, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Shikonin derivatives trigger phenotype reprogramming of B16 mouse melanoma cells" in Biochemistry in Biotechnology": Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023):96,
https://hdl.handle.net/21.15107/rcub_ibiss_6237 .

TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić-Stevanović, Zora
AU  - Jovanović, Ivan
AU  - Pavlović, Sladjana
AU  - Gajović, Nevena
AU  - Mijatović, Sanja
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5297
AB  - Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.
PB  - EFIS Young Immunologist Network (yEFIS Network)
C3  - Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
T1  - Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model
DO  - 10.1002/eji.202270200
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5297
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić-Stevanović, Zora and Jovanović, Ivan and Pavlović, Sladjana and Gajović, Nevena and Mijatović, Sanja and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.",
publisher = "EFIS Young Immunologist Network (yEFIS Network)",
journal = "Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany",
title = "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model",
doi = "10.1002/eji.202270200",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5297"
}

Jelača, S., Drača, D., Dajić-Stevanović, Z., Jovanović, I., Pavlović, S., Gajović, N., Mijatović, S., Arsenijević, N.,& Maksimović-Ivanić, D.. (2022). Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
EFIS Young Immunologist Network (yEFIS Network)., 69.
https://doi.org/10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297

Jelača S, Drača D, Dajić-Stevanović Z, Jovanović I, Pavlović S, Gajović N, Mijatović S, Arsenijević N, Maksimović-Ivanić D. Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany. 2022;:69.
doi:10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

Jelača, Sanja, Drača, Dijana, Dajić-Stevanović, Zora, Jovanović, Ivan, Pavlović, Sladjana, Gajović, Nevena, Mijatović, Sanja, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model" in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany (2022):69,
https://doi.org/10.1002/eji.202270200 .,
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

TY  - CONF
AU  - Dajić-Stevanović, Zora
AU  - Arsenijević, Nebojša
AU  - Kolašinac, Stefan
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Tanić, Nikola
AU  - Tanić, Nasta
AU  - Kanjevac, Tatjana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5293
AB  - Secondary metabolites of plants are known as efficient bioactive compounds used in
prophylaxes and treatment of different disorders and diseases. Plants products have a
long history of use in the treatment of cancer. There are very interesting reports from
ethnobotanical studies, including those from the Balkan region, known for high diversity
of medicinal plants, highlighting the use of various plant drugs as anticancer
agents. More than 3000 plant species were listed as possible anticancer agents. In the last
few decades it was shown that some of plant metabolites exhibit potent and promising
therapeutic effects in cancer treatment. Surprisingly, more than 10,000 phytochemicals
have been identified and used in cancer treatment due to their anti-cancer properties.
The most researched are those belonging to alkaloids, flavonoids, lignans, condensed
tannins, terpenoids (components of essential oils), and others. Additionally, bioactive
compounds could synergistically increase the efficiency of anti-cancer drugs and reduce
their toxic effects. Many successful anti-cancer drugs currently in use, or their
analogues, are plant derived and many more are under clinical trials. This review aims
to address the most reported plants used for cancer treatment in relation to their major
bioactive compounds. Furthermore, possible mechanisms of anticancer activity of
selected plant metabolites will be discussed, including apoptotic pathways, inhibition
of Nuclear Factor-κB (NF-κB), modulation of Wnt/beta-catenin signaling, autophagy,
sensitization of multidrug resistant cancer cells, prevention of cancer cell metastasis
and epigenetic regulation. It is well assumed that high antioxidant activity and anti-inflamamtory
effects of herbal drugs are associated with the anticancer activity. In our
study, several well-known and several autochtonous medicinal plants which expressed
the anticancer potential, will be additionally presented.
AB  - Секундарни метаболити биљака се због свог високог биоактивног дејства ко-
ристе у профилакси и лечењу различитих обољења. Биљне компоненте имају дугу
историју коришћења у третману канцера. Постоје многи занимљиви подаци из
етноботаничких студија, укључујући и оне са подручја Балкана, иначе подруч-
ја познатог по високом диверзитету лековитог биља, о употреби различитих
анти-канцерогених биљака. Више од 3000 биљних врста са анти-канцерогеним
потенцијалом је споменуто у светској литератури. Последњих деценија је пока-
зано да биљни метоболити испољавају високе и обећавајуће терапијске ефекте у
третману канцерогених обољења. Чак 10.000 биоактивних биљних компоненти је
идентификовано као потенцијално анти-канцерогених, међу којима су највише
проучавани алкалоиди, флавоноиди, лигнани, кондензовани танини, терпеноиди
(компоненте етарских уља), и други. Многа биоактивна биљна једињења испо-
љавају синергистичка дејства приликом коришћења са стандардним анти-канце-
рогеним терапеутицима, при чему могу умањити и негативне токсичне ефекте
цитостатика. Наш рад има за циљ да представи најчешће коришћене биљне врсте
у третману канцера у вези са хемијским саставом њихових дрога, тј., доминант-
ним биоактивним компонентама. Такође, биће размотрени и потенцијални ан-
тиканцерогени механизми деловања важнијих биљних секундарних метаболита,
као и њихови ефекти на нпр. апоптозу, инхибицију NF-κB фактора, модулацију
Wnt-бета-катенин сигналних путева, аутофагију, као и позитивне утицаје на ре-
зистентност канцерогених ћелија на различите хемијске агенсе и епигенетичке
одговоре. Иначе је познато да су висока антиоксидативна активност и анти-ин-
фламацијски ефекти биљних дрога повезани са њиховим анти-канцерогеним деј-
ством. Неколико оваквих, у свету добро проучених, као и неколико аутохтоних
врста са нашег подручја, ће бити посебно представљено.
PB  - Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine
C3  - Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
T1  - Plant bioactive compounds in cancer treatment: myth or hope?
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5293
ER  - 

@conference{
author = "Dajić-Stevanović, Zora and Arsenijević, Nebojša and Kolašinac, Stefan and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Tanić, Nikola and Tanić, Nasta and Kanjevac, Tatjana",
year = "2022",
abstract = "Secondary metabolites of plants are known as efficient bioactive compounds used in
prophylaxes and treatment of different disorders and diseases. Plants products have a
long history of use in the treatment of cancer. There are very interesting reports from
ethnobotanical studies, including those from the Balkan region, known for high diversity
of medicinal plants, highlighting the use of various plant drugs as anticancer
agents. More than 3000 plant species were listed as possible anticancer agents. In the last
few decades it was shown that some of plant metabolites exhibit potent and promising
therapeutic effects in cancer treatment. Surprisingly, more than 10,000 phytochemicals
have been identified and used in cancer treatment due to their anti-cancer properties.
The most researched are those belonging to alkaloids, flavonoids, lignans, condensed
tannins, terpenoids (components of essential oils), and others. Additionally, bioactive
compounds could synergistically increase the efficiency of anti-cancer drugs and reduce
their toxic effects. Many successful anti-cancer drugs currently in use, or their
analogues, are plant derived and many more are under clinical trials. This review aims
to address the most reported plants used for cancer treatment in relation to their major
bioactive compounds. Furthermore, possible mechanisms of anticancer activity of
selected plant metabolites will be discussed, including apoptotic pathways, inhibition
of Nuclear Factor-κB (NF-κB), modulation of Wnt/beta-catenin signaling, autophagy,
sensitization of multidrug resistant cancer cells, prevention of cancer cell metastasis
and epigenetic regulation. It is well assumed that high antioxidant activity and anti-inflamamtory
effects of herbal drugs are associated with the anticancer activity. In our
study, several well-known and several autochtonous medicinal plants which expressed
the anticancer potential, will be additionally presented., Секундарни метаболити биљака се због свог високог биоактивног дејства ко-
ристе у профилакси и лечењу различитих обољења. Биљне компоненте имају дугу
историју коришћења у третману канцера. Постоје многи занимљиви подаци из
етноботаничких студија, укључујући и оне са подручја Балкана, иначе подруч-
ја познатог по високом диверзитету лековитог биља, о употреби различитих
анти-канцерогених биљака. Више од 3000 биљних врста са анти-канцерогеним
потенцијалом је споменуто у светској литератури. Последњих деценија је пока-
зано да биљни метоболити испољавају високе и обећавајуће терапијске ефекте у
третману канцерогених обољења. Чак 10.000 биоактивних биљних компоненти је
идентификовано као потенцијално анти-канцерогених, међу којима су највише
проучавани алкалоиди, флавоноиди, лигнани, кондензовани танини, терпеноиди
(компоненте етарских уља), и други. Многа биоактивна биљна једињења испо-
љавају синергистичка дејства приликом коришћења са стандардним анти-канце-
рогеним терапеутицима, при чему могу умањити и негативне токсичне ефекте
цитостатика. Наш рад има за циљ да представи најчешће коришћене биљне врсте
у третману канцера у вези са хемијским саставом њихових дрога, тј., доминант-
ним биоактивним компонентама. Такође, биће размотрени и потенцијални ан-
тиканцерогени механизми деловања важнијих биљних секундарних метаболита,
као и њихови ефекти на нпр. апоптозу, инхибицију NF-κB фактора, модулацију
Wnt-бета-катенин сигналних путева, аутофагију, као и позитивне утицаје на ре-
зистентност канцерогених ћелија на различите хемијске агенсе и епигенетичке
одговоре. Иначе је познато да су висока антиоксидативна активност и анти-ин-
фламацијски ефекти биљних дрога повезани са њиховим анти-канцерогеним деј-
ством. Неколико оваквих, у свету добро проучених, као и неколико аутохтоних
врста са нашег подручја, ће бити посебно представљено.",
publisher = "Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine",
journal = "Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina",
title = "Plant bioactive compounds in cancer treatment: myth or hope?",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5293"
}

Dajić-Stevanović, Z., Arsenijević, N., Kolašinac, S., Maksimović-Ivanić, D., Mijatović, S., Tanić, N., Tanić, N.,& Kanjevac, T.. (2022). Plant bioactive compounds in cancer treatment: myth or hope?. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina
Serbian Society for Immunology, Molecular Oncology and Regenerative Medicine., 59.
https://hdl.handle.net/21.15107/rcub_ibiss_5293

Dajić-Stevanović Z, Arsenijević N, Kolašinac S, Maksimović-Ivanić D, Mijatović S, Tanić N, Tanić N, Kanjevac T. Plant bioactive compounds in cancer treatment: myth or hope?. in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina. 2022;:59.
https://hdl.handle.net/21.15107/rcub_ibiss_5293 .

Dajić-Stevanović, Zora, Arsenijević, Nebojša, Kolašinac, Stefan, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Tanić, Nikola, Tanić, Nasta, Kanjevac, Tatjana, "Plant bioactive compounds in cancer treatment: myth or hope?" in Abstract Book: First Serbian molecular medicine congress; 2022 Jun 16-18; Foča, Bosnia and Herzegovina (2022):59,
https://hdl.handle.net/21.15107/rcub_ibiss_5293 .