@article{
author = "Tepavcevic, Snezana and Vojnović-Milutinović, Danijela and Macut, Djuro and Stojiljkovic, Mojca and Radovanović, Marina and Bozic-Antic, Ivana and Culafic, Tijana and Bjekić-Macut, Jelica and Matić, Gordana and Koricanac, Goran",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma
lipid profile and increased risk for cardiovascular diseases. We
hypothesized that molecular mechanisms underlying cardiac pathology in
PCOS involve changes in expression and subcellular localization of
several key proteins involved in cardiac lipid transport and metabolism,
such as fatty acid transporter CD36, lipin 1, peroxisome
proliferator-activated receptor alpha (PPAR alpha), peroxisome
proliferator-activated receptor gamma coactivator-1 (PGC1), and
carnitine palmitoyltransferase 1 (CPT1). We used the animal model of
PCOS obtained by treating female rats with dihydrotestosterone (DHT).
Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative
enzymes were assessed by Western blot in different cardiac cell
compartments. Cardiac triglycerides (TG) and lipid peroxidation were
also measured. The content of CD36 was decreased in both the cardiac
plasma membranes and intracellular pool. On the other hand, total
content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast
to decreased microsomal lipin 1 content. An increase in nuclear content
of lipin 1 was observed together with elevation of nuclear PPAR alpha
and PGC1, and an increase in CPT1 expression. However, lipid
peroxidation was reduced in the heart, without alterations in
antioxidative enzymes expression and cardiac TG content. The results
indicate that treatment of female rats with DHT is accompanied by a
decrease of fatty acid uptake and a reduction of lipid peroxidation in
the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1
expression suggests that cardiac fatty acid metabolism is shifted toward
mitochondrial beta oxidation.",
journal = "Endocrine",
title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic
ovary syndrome",
number = "1",
volume = "50",
doi = "10.1007/s12020-015-0558-1",
pages = "193-201"
}