TY  - JOUR
AU  - Rehm, Carmen
AU  - Lippert, Kathrin
AU  - Indra, Alexander
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Leopold, Melanie
AU  - Steinbacher, Sophia
AU  - Schachner, Iris
AU  - Campostrini, Lena
AU  - Risslegger, Alexandra
AU  - Farnleitner, Andreas H.
AU  - Kolm, Claudia
AU  - Kirschner, Alexander K T
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5485
AB  - Vibrio cholerae are natural inhabitants of specific aquatic environments. Strains not belonging to serogroups O1 and O139 are usually unable to produce cholera toxin and cause cholera. However, non-toxigenic V. cholerae (NTVC) are able to cause a variety of mild-to-severe human infections (via seafood consumption or recreational activities). The number of unreported cases is considered substantial, as NTVC infections are not notifiable and physicians are mostly unaware of this pathogen. In the northern hemisphere, NTVC infections have been reported to increase due to global warming. In Eastern Europe, climatic and geological conditions favour the existence of inland water-bodies harbouring NTVC. We thus investigated the occurrence of NTVC in nine Serbian natural and artificial lakes and ponds, many of them used for fishing and bathing. With the exception of one highly saline lake, all investigated water-bodies harboured NTVC, ranging from 5.4 × 101 to 1.86 × 104 CFU and 4.5 × 102 to 5.6 × 106 genomic units per 100 ml. The maximum values observed were in the range of bathing waters in other countries, where infections have been reported. Interestingly, 7 out of 39 fully sequenced presumptive V. cholerae isolates were assigned as V. paracholerae, a recently described sister species of V. cholerae. Some clones and sublineages of both V. cholerae and V. paracholerae were shared by different environments indicating an exchange of strains over long distances. Important pathogenicity factors such as hlyA, toxR, and ompU were present in both species. Seasonal monitoring of ponds/lakes used for recreation in Serbia is thus recommended to be prepared for potential occurrence of infections promoted by climate change-induced rise in water temperatures.
PB  - Hoboken: Wiley
T2  - Environmental Microbiology Reports
T1  - First report on the occurrence of Vibrio cholerae nonO1/nonO139 in natural and artificial lakes and ponds in Serbia: Evidence for a long-distance transfer of strains and the presence of Vibrio paracholerae
DO  - 10.1111/1758-2229.13136
ER  - 

@article{
author = "Rehm, Carmen and Lippert, Kathrin and Indra, Alexander and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Leopold, Melanie and Steinbacher, Sophia and Schachner, Iris and Campostrini, Lena and Risslegger, Alexandra and Farnleitner, Andreas H. and Kolm, Claudia and Kirschner, Alexander K T",
year = "2023",
abstract = "Vibrio cholerae are natural inhabitants of specific aquatic environments. Strains not belonging to serogroups O1 and O139 are usually unable to produce cholera toxin and cause cholera. However, non-toxigenic V. cholerae (NTVC) are able to cause a variety of mild-to-severe human infections (via seafood consumption or recreational activities). The number of unreported cases is considered substantial, as NTVC infections are not notifiable and physicians are mostly unaware of this pathogen. In the northern hemisphere, NTVC infections have been reported to increase due to global warming. In Eastern Europe, climatic and geological conditions favour the existence of inland water-bodies harbouring NTVC. We thus investigated the occurrence of NTVC in nine Serbian natural and artificial lakes and ponds, many of them used for fishing and bathing. With the exception of one highly saline lake, all investigated water-bodies harboured NTVC, ranging from 5.4 × 101 to 1.86 × 104 CFU and 4.5 × 102 to 5.6 × 106 genomic units per 100 ml. The maximum values observed were in the range of bathing waters in other countries, where infections have been reported. Interestingly, 7 out of 39 fully sequenced presumptive V. cholerae isolates were assigned as V. paracholerae, a recently described sister species of V. cholerae. Some clones and sublineages of both V. cholerae and V. paracholerae were shared by different environments indicating an exchange of strains over long distances. Important pathogenicity factors such as hlyA, toxR, and ompU were present in both species. Seasonal monitoring of ponds/lakes used for recreation in Serbia is thus recommended to be prepared for potential occurrence of infections promoted by climate change-induced rise in water temperatures.",
publisher = "Hoboken: Wiley",
journal = "Environmental Microbiology Reports",
title = "First report on the occurrence of Vibrio cholerae nonO1/nonO139 in natural and artificial lakes and ponds in Serbia: Evidence for a long-distance transfer of strains and the presence of Vibrio paracholerae",
doi = "10.1111/1758-2229.13136"
}

Rehm, C., Lippert, K., Indra, A., Kolarević, S., Kračun-Kolarević, M., Leopold, M., Steinbacher, S., Schachner, I., Campostrini, L., Risslegger, A., Farnleitner, A. H., Kolm, C.,& Kirschner, A. K. T.. (2023). First report on the occurrence of Vibrio cholerae nonO1/nonO139 in natural and artificial lakes and ponds in Serbia: Evidence for a long-distance transfer of strains and the presence of Vibrio paracholerae. in Environmental Microbiology Reports
Hoboken: Wiley..
https://doi.org/10.1111/1758-2229.13136

Rehm C, Lippert K, Indra A, Kolarević S, Kračun-Kolarević M, Leopold M, Steinbacher S, Schachner I, Campostrini L, Risslegger A, Farnleitner AH, Kolm C, Kirschner AKT. First report on the occurrence of Vibrio cholerae nonO1/nonO139 in natural and artificial lakes and ponds in Serbia: Evidence for a long-distance transfer of strains and the presence of Vibrio paracholerae. in Environmental Microbiology Reports. 2023;.
doi:10.1111/1758-2229.13136 .

Rehm, Carmen, Lippert, Kathrin, Indra, Alexander, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Leopold, Melanie, Steinbacher, Sophia, Schachner, Iris, Campostrini, Lena, Risslegger, Alexandra, Farnleitner, Andreas H., Kolm, Claudia, Kirschner, Alexander K T, "First report on the occurrence of Vibrio cholerae nonO1/nonO139 in natural and artificial lakes and ponds in Serbia: Evidence for a long-distance transfer of strains and the presence of Vibrio paracholerae" in Environmental Microbiology Reports (2023),
https://doi.org/10.1111/1758-2229.13136 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Jovanović Marić, Jovana
AU  - Kirschner, Alexander K T
AU  - Farnleitner, Andreas A H
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S004896972204061X
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9232394
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5072
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Amsterdam: Elsevier
T2  - Science of The Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
SP  - 156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Vojnović-Milutinović, Danijela and Jovanović Marić, Jovana and Kirschner, Alexander K T and Farnleitner, Andreas A H and Linke, Rita and Đukić, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Amsterdam: Elsevier",
journal = "Science of The Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964",
pages = "156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Vojnović-Milutinović, D., Jovanović Marić, J., Kirschner, A. K. T., Farnleitner, A. A. H., Linke, R., Đukić, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment
Amsterdam: Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Vojnović-Milutinović D, Jovanović Marić J, Kirschner AKT, Farnleitner AAH, Linke R, Đukić A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.. in Science of The Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Vojnović-Milutinović, Danijela, Jovanović Marić, Jovana, Kirschner, Alexander K T, Farnleitner, Andreas A H, Linke, Rita, Đukić, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters." in Science of The Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .

TY  - CONF
AU  - Kirschner, Alexander K.T.
AU  - Kittinger, Clemens
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Koller, Michael
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toumi, Marwene
AU  - Farkas, Rosza
AU  - Tóth, Erika
AU  - Kandler, W
AU  - Sulyok, M
AU  - Savio, Domenico
AU  - Farnleitner, Andreas H.
AU  - Zarfel, Gernot
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4731
AB  - The problem of human-induced antimicrobial resistance (acquired due to human activities) is an
emerging concern in aquatic environments. The isolation of (facultative) pathogenic organisms with
acquired antibiotic resistance, even concerning last-line antibiotics, from rivers and lakes, is well
documented throughout the world. In the last few years, there has been a rapid increase in the number
of studies addressing these topics and their possible implications for human health. However, most
studies concentrate on a relatively limited methodology, e.g. applying non-quantitative and either
cultivation-based or direct-detection based molecular biological methods. In addition, often only small
geographic areas and short periods of time are covered. This is one of the reasons why many relevant
questions concerning the occurrence and spread of antimicrobial resistance in aquatic ecosystems still
remain unanswered.
The largest European river water survey (Joint Danube Survey 4) offered a unique opportunity to study
the occurrence and spread of human-induced antimicrobial resistance along the whole Danube River.
Within the research project, isolates from samples from the entire course of the Danube and their
main tributaries, including high frequency sampling at selected locations, have been collected and
analysed. Samples from the water compartment but also from submerged biofilms have been
considered. State-of-the-art quantitative molecular biological techniques and culture-based analyses
targeting different bacterial organisms have been combined with a large set of physical, chemical and
biological parameters to obtain a robust and comprehensive picture of the occurrence and spread of
human-induced antimicrobial resistance in the Danube River.
Based on the findings of this quantitative, integrative study approach, it seems likely to significantly
improve the current understanding on the importance on the spread and stabilization of human -
induced antibiotic resistance in large rivers. The results of this study will also be useful to guide future
monitoring and management strategies.
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Occurrence and spread of human- induced antimicrobial resistance in a large river water system: developing a holistic picture based on the Joint Danube Survey 4 activities
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4731
ER  - 

@conference{
author = "Kirschner, Alexander K.T. and Kittinger, Clemens and Schachner, Iris and Jakwerth, Stefan and Koller, Michael and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toumi, Marwene and Farkas, Rosza and Tóth, Erika and Kandler, W and Sulyok, M and Savio, Domenico and Farnleitner, Andreas H. and Zarfel, Gernot",
year = "2021",
abstract = "The problem of human-induced antimicrobial resistance (acquired due to human activities) is an
emerging concern in aquatic environments. The isolation of (facultative) pathogenic organisms with
acquired antibiotic resistance, even concerning last-line antibiotics, from rivers and lakes, is well
documented throughout the world. In the last few years, there has been a rapid increase in the number
of studies addressing these topics and their possible implications for human health. However, most
studies concentrate on a relatively limited methodology, e.g. applying non-quantitative and either
cultivation-based or direct-detection based molecular biological methods. In addition, often only small
geographic areas and short periods of time are covered. This is one of the reasons why many relevant
questions concerning the occurrence and spread of antimicrobial resistance in aquatic ecosystems still
remain unanswered.
The largest European river water survey (Joint Danube Survey 4) offered a unique opportunity to study
the occurrence and spread of human-induced antimicrobial resistance along the whole Danube River.
Within the research project, isolates from samples from the entire course of the Danube and their
main tributaries, including high frequency sampling at selected locations, have been collected and
analysed. Samples from the water compartment but also from submerged biofilms have been
considered. State-of-the-art quantitative molecular biological techniques and culture-based analyses
targeting different bacterial organisms have been combined with a large set of physical, chemical and
biological parameters to obtain a robust and comprehensive picture of the occurrence and spread of
human-induced antimicrobial resistance in the Danube River.
Based on the findings of this quantitative, integrative study approach, it seems likely to significantly
improve the current understanding on the importance on the spread and stabilization of human -
induced antibiotic resistance in large rivers. The results of this study will also be useful to guide future
monitoring and management strategies.",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Occurrence and spread of human- induced antimicrobial resistance in a large river water system: developing a holistic picture based on the Joint Danube Survey 4 activities",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4731"
}

Kirschner, A. K.T., Kittinger, C., Schachner, I., Jakwerth, S., Koller, M., Kolarević, S., Kračun-Kolarević, M., Toumi, M., Farkas, R., Tóth, E., Kandler, W., Sulyok, M., Savio, D., Farnleitner, A. H.,& Zarfel, G.. (2021). Occurrence and spread of human- induced antimicrobial resistance in a large river water system: developing a holistic picture based on the Joint Danube Survey 4 activities. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 20.
https://hdl.handle.net/21.15107/rcub_ibiss_4731

Kirschner AK, Kittinger C, Schachner I, Jakwerth S, Koller M, Kolarević S, Kračun-Kolarević M, Toumi M, Farkas R, Tóth E, Kandler W, Sulyok M, Savio D, Farnleitner AH, Zarfel G. Occurrence and spread of human- induced antimicrobial resistance in a large river water system: developing a holistic picture based on the Joint Danube Survey 4 activities. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:20.
https://hdl.handle.net/21.15107/rcub_ibiss_4731 .

Kirschner, Alexander K.T., Kittinger, Clemens, Schachner, Iris, Jakwerth, Stefan, Koller, Michael, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toumi, Marwene, Farkas, Rosza, Tóth, Erika, Kandler, W, Sulyok, M, Savio, Domenico, Farnleitner, Andreas H., Zarfel, Gernot, "Occurrence and spread of human- induced antimicrobial resistance in a large river water system: developing a holistic picture based on the Joint Danube Survey 4 activities" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):20,
https://hdl.handle.net/21.15107/rcub_ibiss_4731 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Lundy, Lian
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Đukić, Aleksandar
AU  - Čolić, Jasna
AU  - Nenin, Tanja
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4214
AB  - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
PB  - Elsevier BV
T2  - Science of The Total Environment
T2  - Science of The Total Environment
T1  - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
VL  - 783
DO  - 10.1016/j.scitotenv.2021.146967
SP  - 146967
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Lundy, Lian and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Đukić, Aleksandar and Čolić, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir",
year = "2021",
abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.",
publisher = "Elsevier BV",
journal = "Science of The Total Environment, Science of The Total Environment",
title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters",
volume = "783",
doi = "10.1016/j.scitotenv.2021.146967",
pages = "146967"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Lundy, L., Kirschner, A. K.T., Farnleitner, A. H., Đukić, A., Čolić, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of The Total Environment
Elsevier BV., 783, 146967.
https://doi.org/10.1016/j.scitotenv.2021.146967

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Lundy L, Kirschner AK, Farnleitner AH, Đukić A, Čolić J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of The Total Environment. 2021;783:146967.
doi:10.1016/j.scitotenv.2021.146967 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Lundy, Lian, Kirschner, Alexander K.T., Farnleitner, Andreas H., Đukić, Aleksandar, Čolić, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of The Total Environment, 783 (2021):146967,
https://doi.org/10.1016/j.scitotenv.2021.146967 . .

TY  - CONF
AU  - Koller, Michael
AU  - Hack, Kerstin
AU  - Dielacher, Irina
AU  - Wachter, Julian
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Tóth, Erika
AU  - Savio, Domenico
AU  - Farnleitner, Andreas H.
AU  - Kittinger, Clemens
AU  - Kirschner, Alexander K.T.
AU  - Zarfel, Gernot
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4730
AB  - Introduction
The emerging occurrence of human induced antibiotic resistant bacteria (ARB) is not only limited to
clinical surroundings: they can also be found in the human population, animals and the water
environment. Large rivers are of great concern as regards the spreading of ARB. Thus, this ongoing
study ́s aim is to analyze the major propagation pathways and sources of ARB in the Danube, and to
do a six-year-comparison with data obtained in 2013.
Study design and methods
Within the frame of the 4th Joint Danube Survey (JDS) of the International Commission for the
Protection of the Danube River (ICPDR), water samples were taken at 36 sampling points along the
whole Danube. Escherichia coli as clinically relevant organisms were isolated and tested for their
susceptibility to 20 different antibiotics.
Results
1298 E. coli isolates have been tested so far. 11.33 % of them were multiresistant and 23.19 % were
resistant to one or two classes of antibiotics. The preliminary data show a very similar pattern
compared to the data obtained in 2013 (629 isolates) when 9.70 % of the isolates were multiresistant
and 29.09 % were resistant to one or two classes of antibiotics. Most of the resistances are still to
ampicillin and tetracycline. There are no resistances to carbapenems, colistin, amikacin and tigecycline.
But there is a significant increase in resistances to amoxicillin with clavulanic acid, moxifloxacin and
piperacillin with tazobactam and a significant decrease regarding tetracycline. 21 isolates are
confirmed ESBL-producers while in 2013 there were four.
Discussion
In comparison the data of 2013 and 2019 show a similar pattern regarding multiresistance. Regarding
resistances to single antibiotics significant changes could be determined. However, there are still many
of the isolates to be tested until the final analysis.
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4730
ER  - 

@conference{
author = "Koller, Michael and Hack, Kerstin and Dielacher, Irina and Wachter, Julian and Schachner, Iris and Jakwerth, Stefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Tóth, Erika and Savio, Domenico and Farnleitner, Andreas H. and Kittinger, Clemens and Kirschner, Alexander K.T. and Zarfel, Gernot",
year = "2021",
abstract = "Introduction
The emerging occurrence of human induced antibiotic resistant bacteria (ARB) is not only limited to
clinical surroundings: they can also be found in the human population, animals and the water
environment. Large rivers are of great concern as regards the spreading of ARB. Thus, this ongoing
study ́s aim is to analyze the major propagation pathways and sources of ARB in the Danube, and to
do a six-year-comparison with data obtained in 2013.
Study design and methods
Within the frame of the 4th Joint Danube Survey (JDS) of the International Commission for the
Protection of the Danube River (ICPDR), water samples were taken at 36 sampling points along the
whole Danube. Escherichia coli as clinically relevant organisms were isolated and tested for their
susceptibility to 20 different antibiotics.
Results
1298 E. coli isolates have been tested so far. 11.33 % of them were multiresistant and 23.19 % were
resistant to one or two classes of antibiotics. The preliminary data show a very similar pattern
compared to the data obtained in 2013 (629 isolates) when 9.70 % of the isolates were multiresistant
and 29.09 % were resistant to one or two classes of antibiotics. Most of the resistances are still to
ampicillin and tetracycline. There are no resistances to carbapenems, colistin, amikacin and tigecycline.
But there is a significant increase in resistances to amoxicillin with clavulanic acid, moxifloxacin and
piperacillin with tazobactam and a significant decrease regarding tetracycline. 21 isolates are
confirmed ESBL-producers while in 2013 there were four.
Discussion
In comparison the data of 2013 and 2019 show a similar pattern regarding multiresistance. Regarding
resistances to single antibiotics significant changes could be determined. However, there are still many
of the isolates to be tested until the final analysis.",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4730"
}

Koller, M., Hack, K., Dielacher, I., Wachter, J., Schachner, I., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Tóth, E., Savio, D., Farnleitner, A. H., Kittinger, C., Kirschner, A. K.T.,& Zarfel, G.. (2021). Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4730

Koller M, Hack K, Dielacher I, Wachter J, Schachner I, Jakwerth S, Kolarević S, Kračun-Kolarević M, Tóth E, Savio D, Farnleitner AH, Kittinger C, Kirschner AK, Zarfel G. Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4730 .

Koller, Michael, Hack, Kerstin, Dielacher, Irina, Wachter, Julian, Schachner, Iris, Jakwerth, Stefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Tóth, Erika, Savio, Domenico, Farnleitner, Andreas H., Kittinger, Clemens, Kirschner, Alexander K.T., Zarfel, Gernot, "Non-wild type antibiotic resistant Escherichia coli in the River Danube: a six-year-comparison" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4730 .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović, Jovana
AU  - Ilić, Marija
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, Sandra
AU  - Joksimović, Danijela
AU  - Pešić, Vladimir
AU  - Kirschner, Alexander K. T.
AU  - Linke, Rita
AU  - Ixenmaier, Simone
AU  - Farnleitner, Andreas
AU  - Savio, Domenico
AU  - Reischer, Georg
AU  - Tomić, Nevenka
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - https://link.springer.com/chapter/10.1007/698_2019_420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3952
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer
T2  - The Rivers of Montenegro
T2  - The Rivers of Montenegro
T1  - Microbiological Water Quality of Rivers in Montenegro
DO  - 10.1007/698_2019_420
SP  - 135
EP  - 155
ER  - 

@inbook{
editor = "Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G.",
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović, Jovana and Ilić, Marija and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, Sandra and Joksimović, Danijela and Pešić, Vladimir and Kirschner, Alexander K. T. and Linke, Rita and Ixenmaier, Simone and Farnleitner, Andreas and Savio, Domenico and Reischer, Georg and Tomić, Nevenka and Vuković-Gačić, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer",
journal = "The Rivers of Montenegro, The Rivers of Montenegro",
booktitle = "Microbiological Water Quality of Rivers in Montenegro",
doi = "10.1007/698_2019_420",
pages = "135-155"
}

Pešić, V., Paunović, M., Kostianoy, A. G., Kolarević, S., Kračun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A. K. T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vuković-Gačić, B.. (2020). Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro
Springer., 135-155.
https://doi.org/10.1007/698_2019_420

Pešić V, Paunović M, Kostianoy AG, Kolarević S, Kračun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner AKT, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vuković-Gačić B. Microbiological Water Quality of Rivers in Montenegro. in The Rivers of Montenegro. 2020;:135-155.
doi:10.1007/698_2019_420 .

Pešić, Vladimir, Paunović, Momir, Kostianoy, Andrey G., Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović, Jovana, Ilić, Marija, Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, Sandra, Joksimović, Danijela, Pešić, Vladimir, Kirschner, Alexander K. T., Linke, Rita, Ixenmaier, Simone, Farnleitner, Andreas, Savio, Domenico, Reischer, Georg, Tomić, Nevenka, Vuković-Gačić, Branka, "Microbiological Water Quality of Rivers in Montenegro" in The Rivers of Montenegro (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .