TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander K.T.
AU  - Farnleitner, Andreas H.
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6235
AB  - BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
EP  - 615
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6235
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Đorđević, Ana and Jovanović Marić, Jovana and Vojnović-Milutinović, Danijela and Kirschner, Alexander K.T. and Farnleitner, Andreas H. and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "BACKGROUND
Prokaryotic assays are of great interest in eco/geno-toxicology as they are short-term, simple and cost effective. Application of strains of Salmonella typhimurium with modifications such as increased cell wall permeability and lack of excision repair system is common for the detection of genotoxicity. Various eukaryotic models have been developed lately, such as fish derived cell lines, which can mimic the response of the aquatic organisms. Considering different level of cell organization among these models, difference in sensitivity is expected, yet only few studies address this topic.
OBJECTIVES
The major objective was to investigate genotoxic potential of extracts of surface water of the Danube
and to compare the results obtained in applied prokaryotic and eukaryotic models.
METHODS
Surface water samples from 24 sites along the Danube River were extracted by LVSPE Horizon sampler providing relative enrichment factor (REF) of the extracts of 25,000x. For representative prokaryotic model the SOS/umuC assay with S. typhimurium TA1535/pSK1002 was used. Eukaryotic model comprised assessment of cytotoxicity by MTS test and assessment of genotoxicity by comet assay in ZFL (ZebraFish Liver) cells.
RESULTS
While none of the tested samples has induced SOS response, the result of MTS assay indicated that 11 of 24 samples were cytotoxic. In comet assay, genotoxic potential was found in 9 of 24 samples. Results indicated higher sensitivity of used eukaryotic model in comparison with prokaryotic. Applied prokaryotic assay is often suggested to be used as prescreening tool in diagnostics of genotoxic potential which by our opinion could be misleading.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614-615",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6235"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Đorđević, A., Jovanović Marić, J., Vojnović-Milutinović, D., Kirschner, A. K.T., Farnleitner, A. H., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Đorđević A, Jovanović Marić J, Vojnović-Milutinović D, Kirschner AK, Farnleitner AH, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:614-615.
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Đorđević, Ana, Jovanović Marić, Jovana, Vojnović-Milutinović, Danijela, Kirschner, Alexander K.T., Farnleitner, Andreas H., Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):614-615,
https://hdl.handle.net/21.15107/rcub_ibiss_6235 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Nikolić, Stefan
AU  - Jovanović Marić, Jovana
AU  - Ilić, Marija
AU  - Đukić, Aleksandar
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6589
AB  - U ovom radu ispitivan je stepen kontaminacije vode i plavne zone reke Save na primeru ispusta otpadnih voda kod Beogradskog sajma korišćenjem mikrobioloških indikatora fekalnog zagađenja. Kvantifikacija ukupnih koliforma, fekalnih koliforma i fekalnih enterokoka rađena je pomoću Colilert-18 i Enterolert-E sistema. Analiza broja spora klostridija rađena je membranskom filtracijom u skladu sa standardom ISO 14189:2013. Rezultati mikrobioloških analiza ukazuju na visok stepen kontaminacije vode i plavnog područja na ispitivanom lokalitetu, kao i na potencijalni rizik koje zabeleženo fekalno zagađenje može predstavljati po zdravlje osoba koje ispitivano područje koriste za rekreaciju.
AB  - In this paper, the level of contamination of the Sava River water and floodplain was asssessed using microbiological indicators of fecal pollution. Wastewater outlet near the Belgrade Fair was chosen as a case study. Quantification of total coliforms, faecal coliforms and fecal enterococci was performed using the Colilert-18 and Enterolert-E systems. The analysis of the number of clostridian spores was carried out by membrane filtration in accordance with the ISO 14189 standard. The results of microbiological analyzes indicate a high degree of contamination of the water and floodplain area at the investigated location, as well as the potential risk that detected fecal pollution may represent for the health of persons who use the investigated area for recreation.
PB  - Belgarde: Serbian Association for Water Protection
C3  - Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia
T1  - Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja
T1  - Contamination of the Sava River water and floodplain at the wastewater outlet site assessed by microbiological indicators of faecal pollution
DO  - 77743881
SP  - 191
EP  - 196
ER  - 

@conference{
editor = "Đukić, Aleksandar",
author = "Kračun-Kolarević, Margareta and Nikolić, Stefan and Jovanović Marić, Jovana and Ilić, Marija and Đukić, Aleksandar and Paunović, Momir and Kolarević, Stoimir",
year = "2022",
abstract = "U ovom radu ispitivan je stepen kontaminacije vode i plavne zone reke Save na primeru ispusta otpadnih voda kod Beogradskog sajma korišćenjem mikrobioloških indikatora fekalnog zagađenja. Kvantifikacija ukupnih koliforma, fekalnih koliforma i fekalnih enterokoka rađena je pomoću Colilert-18 i Enterolert-E sistema. Analiza broja spora klostridija rađena je membranskom filtracijom u skladu sa standardom ISO 14189:2013. Rezultati mikrobioloških analiza ukazuju na visok stepen kontaminacije vode i plavnog područja na ispitivanom lokalitetu, kao i na potencijalni rizik koje zabeleženo fekalno zagađenje može predstavljati po zdravlje osoba koje ispitivano područje koriste za rekreaciju., In this paper, the level of contamination of the Sava River water and floodplain was asssessed using microbiological indicators of fecal pollution. Wastewater outlet near the Belgrade Fair was chosen as a case study. Quantification of total coliforms, faecal coliforms and fecal enterococci was performed using the Colilert-18 and Enterolert-E systems. The analysis of the number of clostridian spores was carried out by membrane filtration in accordance with the ISO 14189 standard. The results of microbiological analyzes indicate a high degree of contamination of the water and floodplain area at the investigated location, as well as the potential risk that detected fecal pollution may represent for the health of persons who use the investigated area for recreation.",
publisher = "Belgarde: Serbian Association for Water Protection",
journal = "Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia",
title = "Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja, Contamination of the Sava River water and floodplain at the wastewater outlet site assessed by microbiological indicators of faecal pollution",
doi = "77743881",
pages = "191-196"
}

Đukić, A., Kračun-Kolarević, M., Nikolić, S., Jovanović Marić, J., Ilić, M., Đukić, A., Paunović, M.,& Kolarević, S.. (2022). Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja. in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia
Belgarde: Serbian Association for Water Protection., 191-196.
https://doi.org/77743881

Đukić A, Kračun-Kolarević M, Nikolić S, Jovanović Marić J, Ilić M, Đukić A, Paunović M, Kolarević S. Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja. in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia. 2022;:191-196.
doi:77743881 .

Đukić, Aleksandar, Kračun-Kolarević, Margareta, Nikolić, Stefan, Jovanović Marić, Jovana, Ilić, Marija, Đukić, Aleksandar, Paunović, Momir, Kolarević, Stoimir, "Procena kontaminacije vode i plavne zone reke Save na mestu ispusta otpadnih voda korišćenjem mikrobioloških indikatora fekalnog zagađenja" in Conferenece proceedings: 51st Annual Conference of the Serbian Water Pollution Control Society: Water 2022; 2022 Oct 26-28; Vrnjačka Banja, Serbia (2022):191-196,
https://doi.org/77743881 . .