TY  - JOUR
AU  - Prvulović, Milica
AU  - Milanović, Desanka
AU  - Vujović, Predrag
AU  - Jović, Milena
AU  - Kanazir, Selma
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
PY  - 2021
UR  - https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glab353/6433769
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5436
AB  - The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.
T2  - The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences
T1  - Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.
IS  - 5
VL  - 77
DO  - 10.1093/gerona/glab353
SP  - glab353
SP  - 947
EP  - 955
ER  - 

@article{
author = "Prvulović, Milica and Milanović, Desanka and Vujović, Predrag and Jović, Milena and Kanazir, Selma and Todorović, Smilja and Mladenović, Aleksandra",
year = "2021",
abstract = "The current study aims to determine the potential benefits of calorie restriction (CR), one of the most promising paradigms for life span and healthspan extension, on cognitive performances in female Wistar rats during aging. As a measure of a healthspan, we evaluated the effects of different onset and duration of CR on frailty level. Female Wistar rats were exposed to either ad libitum (AL) or CR (60% of AL daily intake) food intake during aging. Two different CR protocols were used, life-long CR with an early-onset that started at the adult stage (6 months) and 3-month-long CR, started at the middle (15 months) and late-middle (21 months) age, thus defined as a late-onset CR. The effects of CR were evaluated using open-field, Y-maze, and novel object recognition tests. We broadened 2 tools for frailty assessment currently in use for experimental animals, and in alignment with our previous study, we created a physical-cognitive frailty tool that combines both physical and cognitive performances. Our results clearly showed that CR effects are highly dependent on CR duration and onset. While a life-long restriction with an early-onset has been proven as protective and beneficial, short-term restriction introduced at late age significantly worsens an animal's behavior and frailty. These results complement our previous study conducted in males and contribute to the understanding of sex differences in a response to CR during aging.",
journal = "The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences",
title = "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.",
number = "5",
volume = "77",
doi = "10.1093/gerona/glab353",
pages = "glab353-947-955"
}

Prvulović, M., Milanović, D., Vujović, P., Jović, M., Kanazir, S., Todorović, S.,& Mladenović, A.. (2021). Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77(5), glab353-955.
https://doi.org/10.1093/gerona/glab353

Prvulović M, Milanović D, Vujović P, Jović M, Kanazir S, Todorović S, Mladenović A. Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats.. in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences. 2021;77(5):glab353-955.
doi:10.1093/gerona/glab353 .

Prvulović, Milica, Milanović, Desanka, Vujović, Predrag, Jović, Milena, Kanazir, Selma, Todorović, Smilja, Mladenović, Aleksandra, "Late-Onset Calorie Restriction Worsens Cognitive Performances and Increases Frailty Level in Female Wistar Rats." in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 77, no. 5 (2021):glab353-955,
https://doi.org/10.1093/gerona/glab353 . .

TY  - JOUR
AU  - Paunović, Zoran
AU  - Stanojević, Ivan
AU  - Abazović, Džihan
AU  - Rakić, Mia
AU  - Stanković, Nikola
AU  - Đukić, Mirjana
AU  - Milutinović, Sanja
AU  - Starčević, Srđan
AU  - Šupić, Gordana
AU  - Vojvodić, Danilo
AU  - Jović, Milena
AU  - Marić, Dusan
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0042-84501900062P
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4185
AB  - Background/Aim. Recent studies indicate that adipokines have an important role in bone physiology and pathology. Recent data indicate that adipokine leptin functions as a regulator of bone growth at multiple levels, systemically and locally. So far, it has been shown that leptin influences bone volume and bone mineral density in a population with metabolic and/or hormonal abnormality. Data concerning leptin values in non-obese children with fractures are scarce. Methods. This study included 93 non-obese children with long bone fractures (LBF), 14 children with short bone fractures (SBF), and 19 healthy children. Leptin concentration was determined in two blood samples (day 0 and day 21) and analyzed according to gender, fracture type, anatomical localization of the fracture, fracture topography, callus formation, and the healing outcome. Results. Children with LBF demonstrated significantly increased leptin levels compared to the control group (both day 0/day 21). In the control group, girls had significantly more leptin than boys. Leptin value was significantly influenced by anatomical localization since boys and girls with humerus fracture and girls with femur fracture had the highest average leptin concentration in the initial sample. Boys with incomplete callus formation had the highest leptin concentration (both day 0 /day 21), significantly elevated compared to boys? samples in the control group, boys? samples with an intermediary and well-formed callus, and also increased compared to the initial samples of girls with incomplete callus. Better callus formation in girls was associated with an increment of leptin concentrations in the second over the initial sample. Girls with partially and satisfactorily formed callus had significantly increased leptin concentration in the second sample (day 21) compared to the boys? group. Conclusion. Leptin concentration was significantly increased (both samples) in children with LBF compared to children with SBF and corresponding controls. Leptin concentration was highly influenced by gender. High blood leptin concentrations in boys or low leptin concentrations in girls immediately upon fracture could be used to identify groups of children with incomplete callus formation.
T2  - Vojnosanitetski pregled
T2  - Vojnosanitetski pregled
T1  - Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures
T1  - Povezanost tipa preloma kosti i stepena formiranja kalusa sa koncentracijom leptina kod dece sa prelomima dugih kostiju
IS  - 2
VL  - 78
DO  - 10.2298/VSP190314062P
SP  - 192
EP  - 201
ER  - 

@article{
author = "Paunović, Zoran and Stanojević, Ivan and Abazović, Džihan and Rakić, Mia and Stanković, Nikola and Đukić, Mirjana and Milutinović, Sanja and Starčević, Srđan and Šupić, Gordana and Vojvodić, Danilo and Jović, Milena and Marić, Dusan",
year = "2021",
abstract = "Background/Aim. Recent studies indicate that adipokines have an important role in bone physiology and pathology. Recent data indicate that adipokine leptin functions as a regulator of bone growth at multiple levels, systemically and locally. So far, it has been shown that leptin influences bone volume and bone mineral density in a population with metabolic and/or hormonal abnormality. Data concerning leptin values in non-obese children with fractures are scarce. Methods. This study included 93 non-obese children with long bone fractures (LBF), 14 children with short bone fractures (SBF), and 19 healthy children. Leptin concentration was determined in two blood samples (day 0 and day 21) and analyzed according to gender, fracture type, anatomical localization of the fracture, fracture topography, callus formation, and the healing outcome. Results. Children with LBF demonstrated significantly increased leptin levels compared to the control group (both day 0/day 21). In the control group, girls had significantly more leptin than boys. Leptin value was significantly influenced by anatomical localization since boys and girls with humerus fracture and girls with femur fracture had the highest average leptin concentration in the initial sample. Boys with incomplete callus formation had the highest leptin concentration (both day 0 /day 21), significantly elevated compared to boys? samples in the control group, boys? samples with an intermediary and well-formed callus, and also increased compared to the initial samples of girls with incomplete callus. Better callus formation in girls was associated with an increment of leptin concentrations in the second over the initial sample. Girls with partially and satisfactorily formed callus had significantly increased leptin concentration in the second sample (day 21) compared to the boys? group. Conclusion. Leptin concentration was significantly increased (both samples) in children with LBF compared to children with SBF and corresponding controls. Leptin concentration was highly influenced by gender. High blood leptin concentrations in boys or low leptin concentrations in girls immediately upon fracture could be used to identify groups of children with incomplete callus formation.",
journal = "Vojnosanitetski pregled, Vojnosanitetski pregled",
title = "Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures, Povezanost tipa preloma kosti i stepena formiranja kalusa sa koncentracijom leptina kod dece sa prelomima dugih kostiju",
number = "2",
volume = "78",
doi = "10.2298/VSP190314062P",
pages = "192-201"
}

Paunović, Z., Stanojević, I., Abazović, D., Rakić, M., Stanković, N., Đukić, M., Milutinović, S., Starčević, S., Šupić, G., Vojvodić, D., Jović, M.,& Marić, D.. (2021). Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures. in Vojnosanitetski pregled, 78(2), 192-201.
https://doi.org/10.2298/VSP190314062P

Paunović Z, Stanojević I, Abazović D, Rakić M, Stanković N, Đukić M, Milutinović S, Starčević S, Šupić G, Vojvodić D, Jović M, Marić D. Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures. in Vojnosanitetski pregled. 2021;78(2):192-201.
doi:10.2298/VSP190314062P .

Paunović, Zoran, Stanojević, Ivan, Abazović, Džihan, Rakić, Mia, Stanković, Nikola, Đukić, Mirjana, Milutinović, Sanja, Starčević, Srđan, Šupić, Gordana, Vojvodić, Danilo, Jović, Milena, Marić, Dusan, "Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures" in Vojnosanitetski pregled, 78, no. 2 (2021):192-201,
https://doi.org/10.2298/VSP190314062P . .