TY  - JOUR
AU  - Mojić, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R.
AU  - Stanic, Marina
AU  - Mijatović, Sanja
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2177
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study
IS  - 5955
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojić, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R. and Stanic, Marina and Mijatović, Sanja and Spasojevic, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study",
number = "5955",
volume = "4",
doi = "10.1038/srep05955"
}

Mojić, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanic, M., Mijatović, S.,& Spasojevic, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports, 4(5955).
https://doi.org/10.1038/srep05955

Mojić M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanic M, Mijatović S, Spasojevic I. Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports. 2014;4(5955).
doi:10.1038/srep05955 .

Mojić, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R., Stanic, Marina, Mijatović, Sanja, Spasojevic, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study" in Scientific Reports, 4, no. 5955 (2014),
https://doi.org/10.1038/srep05955 . .

TY  - JOUR
AU  - Nikolić, Milan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Stanić, Dragana
AU  - Vranić, Danijela
AU  - Jones, David R.
AU  - Niketić, Vesna
AU  - Spasić, Mihajlo
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/552
AB  - In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands. .
AB  - U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin (Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze, glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb) u eritrocitima 60 ljudi, podeljenih u dve grupe na osnovu količine Hb-Ch. Rezultati pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo metHb. Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom. U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima prilagođava svoju organizaciju prema zahtevima iz svog okruženja. .
T2  - Journal of the Serbian Chemical Society
T1  - Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?
T1  - Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?
IS  - 4
VL  - 72
SP  - 339
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_552
ER  - 

@article{
author = "Nikolić, Milan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Stanić, Dragana and Vranić, Danijela and Jones, David R. and Niketić, Vesna and Spasić, Mihajlo",
year = "2007, 2007",
abstract = "In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands. ., U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin (Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze, glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb) u eritrocitima 60 ljudi, podeljenih u dve grupe na osnovu količine Hb-Ch. Rezultati pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo metHb. Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom. U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima prilagođava svoju organizaciju prema zahtevima iz svog okruženja. .",
journal = "Journal of the Serbian Chemical Society",
title = "Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?, Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?",
number = "4",
volume = "72",
pages = "339-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_552"
}

Nikolić, M., Blagojević, D., Nikolić-Kokić, A., Stanić, D., Vranić, D., Jones, D. R., Niketić, V.,& Spasić, M.. (2007). Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?. in Journal of the Serbian Chemical Society, 72(4), 339-345.
https://hdl.handle.net/21.15107/rcub_ibiss_552

Nikolić M, Blagojević D, Nikolić-Kokić A, Stanić D, Vranić D, Jones DR, Niketić V, Spasić M. Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?. in Journal of the Serbian Chemical Society. 2007;72(4):339-345.
https://hdl.handle.net/21.15107/rcub_ibiss_552 .

Nikolić, Milan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Stanić, Dragana, Vranić, Danijela, Jones, David R., Niketić, Vesna, Spasić, Mihajlo, "Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?" in Journal of the Serbian Chemical Society, 72, no. 4 (2007):339-345,
https://hdl.handle.net/21.15107/rcub_ibiss_552 .