TY  - JOUR
AU  - Stanković Neđić, Milena
AU  - Župunski, Milan
AU  - Orlović, Saša
AU  - Kovačević, Branislav
AU  - Kebert, Marko
AU  - Vaštag, Erna
AU  - Miljković, Danijela
AU  - Gutalj, Marko
AU  - Gavranović Markić, Anđelina
AU  - Stojnić, Srđan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5476
AB  - Wild  cherry  (Prunus  avium  L.)  is  a  multi-purpose  tree  species  with great ecological and economic importance for European forestry.   Evaluating   this   species   phenotypic   diversity   and   quantitative  traits  characterization  is  of  great  importance  to  define its genetic resources conservation and breeding strate-gies. In this work, variations of physiological, biochemical, ana-tomical  and  morphological  traits  of  one-year-old  wild  cherry  seedlings  were  evaluated  within  and  among  populations  to  distinguish  and  characterize  their  phenotypic  portfolio.  We  observed significant differences at the intra- and inter-popula-tion levels considering both biochemical and physiological leaf traits,  whereas  differences  in  morphological  and  anatomical  traits  were  found  to  be  significant  only  among  half-sib  lines  within populations (i.e. intra-population level). With a multiva-riate  approach,  we  explored  the  inter-population  specificity  and found out that the tiered approach spanning from organ morphology,  across  physiological  scale,  to  the  biochemical  level  gave  out  enough  power  to  discriminate  between  diffe-rent  populations,  and  their  acquisition  and  resource-use  stra-tegies. Moreover, stepwise discriminative analysis showed that radical scavenger capacity against 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic  acid)  (ABTS•+)  and  water-use  efficiency  contributed to discrimination of studied populations to the lar-gest extend. Lastly, our study highlights the robustness of cer-tain functional traits, such as ABTS•+, water-use efficiency, net photosynthesis,   total   flavonoid   content,   width   of   stomata   guard cell, and stomatal aperture length, which could be con-sidered as a proxy to discriminate between wild cherry popula-tions and assess phenotypic diversity.
PB  - Warsaw: Sciendo
T2  - Silvae Genetica
T1  - Assessment of the phenotypic diversity of wild cherry (Prunus avium L.) populations and halfsib lines by multivariate statistical analyses
IS  - 1
VL  - 71
DO  - https://doi.org/10.2478/sg-2022-0014
DO  - 10.2478/sg-2022-0014
SP  - 116
EP  - 127
ER  - 

@article{
author = "Stanković Neđić, Milena and Župunski, Milan and Orlović, Saša and Kovačević, Branislav and Kebert, Marko and Vaštag, Erna and Miljković, Danijela and Gutalj, Marko and Gavranović Markić, Anđelina and Stojnić, Srđan",
year = "2022",
abstract = "Wild  cherry  (Prunus  avium  L.)  is  a  multi-purpose  tree  species  with great ecological and economic importance for European forestry.   Evaluating   this   species   phenotypic   diversity   and   quantitative  traits  characterization  is  of  great  importance  to  define its genetic resources conservation and breeding strate-gies. In this work, variations of physiological, biochemical, ana-tomical  and  morphological  traits  of  one-year-old  wild  cherry  seedlings  were  evaluated  within  and  among  populations  to  distinguish  and  characterize  their  phenotypic  portfolio.  We  observed significant differences at the intra- and inter-popula-tion levels considering both biochemical and physiological leaf traits,  whereas  differences  in  morphological  and  anatomical  traits  were  found  to  be  significant  only  among  half-sib  lines  within populations (i.e. intra-population level). With a multiva-riate  approach,  we  explored  the  inter-population  specificity  and found out that the tiered approach spanning from organ morphology,  across  physiological  scale,  to  the  biochemical  level  gave  out  enough  power  to  discriminate  between  diffe-rent  populations,  and  their  acquisition  and  resource-use  stra-tegies. Moreover, stepwise discriminative analysis showed that radical scavenger capacity against 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic  acid)  (ABTS•+)  and  water-use  efficiency  contributed to discrimination of studied populations to the lar-gest extend. Lastly, our study highlights the robustness of cer-tain functional traits, such as ABTS•+, water-use efficiency, net photosynthesis,   total   flavonoid   content,   width   of   stomata   guard cell, and stomatal aperture length, which could be con-sidered as a proxy to discriminate between wild cherry popula-tions and assess phenotypic diversity.",
publisher = "Warsaw: Sciendo",
journal = "Silvae Genetica",
title = "Assessment of the phenotypic diversity of wild cherry (Prunus avium L.) populations and halfsib lines by multivariate statistical analyses",
number = "1",
volume = "71",
doi = "https://doi.org/10.2478/sg-2022-0014, 10.2478/sg-2022-0014",
pages = "116-127"
}

Stanković Neđić, M., Župunski, M., Orlović, S., Kovačević, B., Kebert, M., Vaštag, E., Miljković, D., Gutalj, M., Gavranović Markić, A.,& Stojnić, S.. (2022). Assessment of the phenotypic diversity of wild cherry (Prunus avium L.) populations and halfsib lines by multivariate statistical analyses. in Silvae Genetica
Warsaw: Sciendo., 71(1), 116-127.
https://doi.org/https://doi.org/10.2478/sg-2022-0014

Stanković Neđić M, Župunski M, Orlović S, Kovačević B, Kebert M, Vaštag E, Miljković D, Gutalj M, Gavranović Markić A, Stojnić S. Assessment of the phenotypic diversity of wild cherry (Prunus avium L.) populations and halfsib lines by multivariate statistical analyses. in Silvae Genetica. 2022;71(1):116-127.
doi:https://doi.org/10.2478/sg-2022-0014 .

Stanković Neđić, Milena, Župunski, Milan, Orlović, Saša, Kovačević, Branislav, Kebert, Marko, Vaštag, Erna, Miljković, Danijela, Gutalj, Marko, Gavranović Markić, Anđelina, Stojnić, Srđan, "Assessment of the phenotypic diversity of wild cherry (Prunus avium L.) populations and halfsib lines by multivariate statistical analyses" in Silvae Genetica, 71, no. 1 (2022):116-127,
https://doi.org/https://doi.org/10.2478/sg-2022-0014 . .

TY  - JOUR
AU  - Stojnić, Srđan
AU  - Viscosi, Vincenzo
AU  - Marković, Milena
AU  - Ivanković, Mladen
AU  - Orlović, Saša
AU  - Tognetti, Roberto
AU  - Cocozza, Claudia
AU  - Vasić, Verica
AU  - Loy, Anna
PY  - 2021
UR  - https://link.springer.com/10.1007/s00468-021-02224-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4638
AB  - Spatial and environmental patterns of European beech (Fagus sylvatica L.) leaf size and shape variations were studied using landmark-based geometric morphometrics. The study involved eight provenances originating from three biogeographic regions (i.e., Alpine, Continental, and Pannonian), spanning across six European countries (Bosnia and Herzegovina, Croatia, Germany, Hungary, Romania and Serbia). All specimens were cultivated in a common garden experiment. The symmetric component of leaf shape variation was analyzed by Procrustes ANOVA and multi‐variate analyses [principal component analysis and canonical variate analysis (CVA)], whereas MANOVA was used to examine asymmetry. Partial least square (PLS) analysis was used to assess the covariation between leaf shape and size, and geographical position and environmental variables at the sites of provenance origin, respectively. A highly observed phenotypic variation for the shape and size of leaf both within and among provenances, indicates a strong local adaptation of provenances within the species natural range. CVA revealed the existence of two clusters of provenances based on the leaf shape, i.e., the first group included provenances originating from Balkan Peninsula and Central Europe, while the second group consisted of two Romanian provenances and the northernmost provenance from Germany. Likewise, PLS evidenced that leaf shape was spatially structured along latitudinal (− 0.64) and longitudinal (0.60) gradients, with southern provenances having longer and narrower (ovate) lamina compared to the northern ones. In contrast, no correlation was found between centroid size and spatial and environmental variables. Results suggest the presence of direct selection favoring a leaf shape that likely safeguards trees from heat and water loses under less favorable growing conditions.
PB  - Heidelberg: Springer Science and Business Media Deutschland GmbH
T2  - Trees
T1  - Spatial patterns of leaf shape variation in European beech (Fagus sylvatica L.) provenances
DO  - 10.1007/s00468-021-02224-6
ER  - 

@article{
author = "Stojnić, Srđan and Viscosi, Vincenzo and Marković, Milena and Ivanković, Mladen and Orlović, Saša and Tognetti, Roberto and Cocozza, Claudia and Vasić, Verica and Loy, Anna",
year = "2021",
abstract = "Spatial and environmental patterns of European beech (Fagus sylvatica L.) leaf size and shape variations were studied using landmark-based geometric morphometrics. The study involved eight provenances originating from three biogeographic regions (i.e., Alpine, Continental, and Pannonian), spanning across six European countries (Bosnia and Herzegovina, Croatia, Germany, Hungary, Romania and Serbia). All specimens were cultivated in a common garden experiment. The symmetric component of leaf shape variation was analyzed by Procrustes ANOVA and multi‐variate analyses [principal component analysis and canonical variate analysis (CVA)], whereas MANOVA was used to examine asymmetry. Partial least square (PLS) analysis was used to assess the covariation between leaf shape and size, and geographical position and environmental variables at the sites of provenance origin, respectively. A highly observed phenotypic variation for the shape and size of leaf both within and among provenances, indicates a strong local adaptation of provenances within the species natural range. CVA revealed the existence of two clusters of provenances based on the leaf shape, i.e., the first group included provenances originating from Balkan Peninsula and Central Europe, while the second group consisted of two Romanian provenances and the northernmost provenance from Germany. Likewise, PLS evidenced that leaf shape was spatially structured along latitudinal (− 0.64) and longitudinal (0.60) gradients, with southern provenances having longer and narrower (ovate) lamina compared to the northern ones. In contrast, no correlation was found between centroid size and spatial and environmental variables. Results suggest the presence of direct selection favoring a leaf shape that likely safeguards trees from heat and water loses under less favorable growing conditions.",
publisher = "Heidelberg: Springer Science and Business Media Deutschland GmbH",
journal = "Trees",
title = "Spatial patterns of leaf shape variation in European beech (Fagus sylvatica L.) provenances",
doi = "10.1007/s00468-021-02224-6"
}

Stojnić, S., Viscosi, V., Marković, M., Ivanković, M., Orlović, S., Tognetti, R., Cocozza, C., Vasić, V.,& Loy, A.. (2021). Spatial patterns of leaf shape variation in European beech (Fagus sylvatica L.) provenances. in Trees
Heidelberg: Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s00468-021-02224-6

Stojnić S, Viscosi V, Marković M, Ivanković M, Orlović S, Tognetti R, Cocozza C, Vasić V, Loy A. Spatial patterns of leaf shape variation in European beech (Fagus sylvatica L.) provenances. in Trees. 2021;.
doi:10.1007/s00468-021-02224-6 .

Stojnić, Srđan, Viscosi, Vincenzo, Marković, Milena, Ivanković, Mladen, Orlović, Saša, Tognetti, Roberto, Cocozza, Claudia, Vasić, Verica, Loy, Anna, "Spatial patterns of leaf shape variation in European beech (Fagus sylvatica L.) provenances" in Trees (2021),
https://doi.org/10.1007/s00468-021-02224-6 . .

TY  - JOUR
AU  - Miljković, Danijela
AU  - Marković, Milena
AU  - Orlović, Saša
AU  - Stanković Neđić, Milena
AU  - Kesić, Lazar
AU  - Stojnić, Srđan
PY  - 2019
UR  - http://link.springer.com/10.1007/s00035-019-00227-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3509
AB  - Leaf shape variations and developmental instability were examined for the first time in natural populations of Prunus avium (L.) L. in the central Balkan region (Bosnia and Herzegovina) at different elevational points, from 230 to 1177 m. above sea level. Geometric morphometric tools were applied to assess the variability of leaf shapes and sizes, while a fluctuating asymmetry leaf index was used as a measure of leaf developmental instability. According to the results of canonical variate analysis for the symmetric component of shape variation and hierarchical analysis of variance for centroid size, the studied populations could be partially differentiated into three groups. The co-variation between leaf form (shape and size) and climate variables was significant, estimated by two-block partial least square analysis. Climate variables (the sum of precipitation in May and the De Martonne aridity index) mostly influenced leaf shape and size. A population situated at the highest elevation had the highest value for fluctuating asymmetry leaf index, which was an indication of developmental instability. High natural variability and interpopulation differences were observed for all studied leaf traits (leaf shape, centroid size, fluctuating asymmetry leaf index, leaf area, leaf length and width, petiole length). For well-known traditional morphometric measures (leaf area, leaf length, leaf width, and petiole length) in accordance with previous studies, intrapopulation variability was greater than interpopulation variability. For centroid size and the fluctuating asymmetry leaf index (measures used in geometric morphometrics) variability was higher among populations than within them. This indicates that geometric morphometrics could give new insights into infra-specific variability.
T2  - Alpine Botany
T1  - Wild cherry (Prunus avium (L.) L.) leaf shape and size variations in natural populations at different elevations
IS  - 2
VL  - 129
DO  - 10.1007/s00035-019-00227-1
SP  - 163
EP  - 174
ER  - 

@article{
author = "Miljković, Danijela and Marković, Milena and Orlović, Saša and Stanković Neđić, Milena and Kesić, Lazar and Stojnić, Srđan",
year = "2019",
abstract = "Leaf shape variations and developmental instability were examined for the first time in natural populations of Prunus avium (L.) L. in the central Balkan region (Bosnia and Herzegovina) at different elevational points, from 230 to 1177 m. above sea level. Geometric morphometric tools were applied to assess the variability of leaf shapes and sizes, while a fluctuating asymmetry leaf index was used as a measure of leaf developmental instability. According to the results of canonical variate analysis for the symmetric component of shape variation and hierarchical analysis of variance for centroid size, the studied populations could be partially differentiated into three groups. The co-variation between leaf form (shape and size) and climate variables was significant, estimated by two-block partial least square analysis. Climate variables (the sum of precipitation in May and the De Martonne aridity index) mostly influenced leaf shape and size. A population situated at the highest elevation had the highest value for fluctuating asymmetry leaf index, which was an indication of developmental instability. High natural variability and interpopulation differences were observed for all studied leaf traits (leaf shape, centroid size, fluctuating asymmetry leaf index, leaf area, leaf length and width, petiole length). For well-known traditional morphometric measures (leaf area, leaf length, leaf width, and petiole length) in accordance with previous studies, intrapopulation variability was greater than interpopulation variability. For centroid size and the fluctuating asymmetry leaf index (measures used in geometric morphometrics) variability was higher among populations than within them. This indicates that geometric morphometrics could give new insights into infra-specific variability.",
journal = "Alpine Botany",
title = "Wild cherry (Prunus avium (L.) L.) leaf shape and size variations in natural populations at different elevations",
number = "2",
volume = "129",
doi = "10.1007/s00035-019-00227-1",
pages = "163-174"
}

Miljković, D., Marković, M., Orlović, S., Stanković Neđić, M., Kesić, L.,& Stojnić, S.. (2019). Wild cherry (Prunus avium (L.) L.) leaf shape and size variations in natural populations at different elevations. in Alpine Botany, 129(2), 163-174.
https://doi.org/10.1007/s00035-019-00227-1

Miljković D, Marković M, Orlović S, Stanković Neđić M, Kesić L, Stojnić S. Wild cherry (Prunus avium (L.) L.) leaf shape and size variations in natural populations at different elevations. in Alpine Botany. 2019;129(2):163-174.
doi:10.1007/s00035-019-00227-1 .

Miljković, Danijela, Marković, Milena, Orlović, Saša, Stanković Neđić, Milena, Kesić, Lazar, Stojnić, Srđan, "Wild cherry (Prunus avium (L.) L.) leaf shape and size variations in natural populations at different elevations" in Alpine Botany, 129, no. 2 (2019):163-174,
https://doi.org/10.1007/s00035-019-00227-1 . .

TY  - JOUR
AU  - Batos, Branislava
AU  - Vilotić, Dragica
AU  - Orlović, Saša
AU  - Miljković, Danijela
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/269
AB  - The research involved the examination of inter- and intra-population variation of stomatal traits: stomatal density, stomatal length and width, stomatal pore surface, potential conductance index and stomatal shape coefficient, in Quercus robur L. leaves. The research was conducted in northern Serbia and included five populations ('Ada Ciganlija', 'Bojčinska šuma', 'Subotica', 'Sombor' and 'Vršac'). The stomatal characteristics were examined in fully expanded leaves, from two leaf positions - the sun-exposed and shaded side of the tree. The leaf position in the tree crown, forming a part of the phenotypic variance, was relevant for the stomatal dimension traits. Within populations, the differences between the genotypes (i.e. trees), were relevant for all analyzed traits. On the basis of the analysis of the inter-populational differences, the 'Bojčinska šuma' population had a statistically significantly lower stomatal density in comparison to the other populations.
T2  - Archives of Biological Sciences
T1  - Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in Northern Serbia
IS  - 4
VL  - 62
SP  - 1125
EP  - 1136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_269
ER  - 

@article{
author = "Batos, Branislava and Vilotić, Dragica and Orlović, Saša and Miljković, Danijela",
year = "2010, 2010",
abstract = "The research involved the examination of inter- and intra-population variation of stomatal traits: stomatal density, stomatal length and width, stomatal pore surface, potential conductance index and stomatal shape coefficient, in Quercus robur L. leaves. The research was conducted in northern Serbia and included five populations ('Ada Ciganlija', 'Bojčinska šuma', 'Subotica', 'Sombor' and 'Vršac'). The stomatal characteristics were examined in fully expanded leaves, from two leaf positions - the sun-exposed and shaded side of the tree. The leaf position in the tree crown, forming a part of the phenotypic variance, was relevant for the stomatal dimension traits. Within populations, the differences between the genotypes (i.e. trees), were relevant for all analyzed traits. On the basis of the analysis of the inter-populational differences, the 'Bojčinska šuma' population had a statistically significantly lower stomatal density in comparison to the other populations.",
journal = "Archives of Biological Sciences",
title = "Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in Northern Serbia",
number = "4",
volume = "62",
pages = "1125-1136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_269"
}

Batos, B., Vilotić, D., Orlović, S.,& Miljković, D.. (2010). Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in Northern Serbia. in Archives of Biological Sciences, 62(4), 1125-1136.
https://hdl.handle.net/21.15107/rcub_ibiss_269

Batos B, Vilotić D, Orlović S, Miljković D. Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in Northern Serbia. in Archives of Biological Sciences. 2010;62(4):1125-1136.
https://hdl.handle.net/21.15107/rcub_ibiss_269 .

Batos, Branislava, Vilotić, Dragica, Orlović, Saša, Miljković, Danijela, "Inter and intra-population variation of leaf stomatal traits of Quercus robur L. in Northern Serbia" in Archives of Biological Sciences, 62, no. 4 (2010):1125-1136,
https://hdl.handle.net/21.15107/rcub_ibiss_269 .