TY  - JOUR
AU  - Brisevac, Dusica
AU  - Adzic, Marija
AU  - Laketa, Danijela
AU  - Parabucki, Ana
AU  - Milosevic, Milena
AU  - Lavrnja, Irena
AU  - Bjelobaba, Ivana
AU  - Sevigny, Jean
AU  - Kipp, Markus
AU  - Nedeljkovic, Nadezda
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2349
AB  - Extracellular ATP (eATP) acts as a danger-associated molecular pattern
   which induces reactive response of astrocytes after brain insult,
   including morphological remodeling of astrocytes, proliferation,
   chemotaxis, and release of proinflammatory cytokines. The responses
   induced by eATP are under control of ecto-nucleotidases, which catalyze
   sequential hydrolysis of ATP to adenosine. In the mammalian brain,
   ecto-nucleotidases comprise three enzyme families: ecto-nucleoside
   triphosphate diphosphohydrolases 1-3 (NTPDase1-3), ecto-nucleotide
   pyrophosphatase/phospodiesterases 1-3 (NPP1-3), and ecto-5'-nucleotidase
   (eN), which crucially determine ATP/adenosine ratio in the pericellular
   milieu. Altered expression of ecto-nucleotidases has been demonstrated
   in several experimental models of human brain dysfunctions. In the
   present study, we have explored the pattern of NTPDase1-3, NPP1-3, and
   eN expression by cultured cortical astrocytes challenged with 1 mmol/L
   ATP (eATP). At the transcriptional level, eATP upregulated expression of
   NTPDase1, NTPDase2, NPP2, and eN, while, at translational and functional
   levels, these were paralleled only by the induction of NTPDase2 and eN.
   Additionally, eATP altered membrane topology of eN, from clusters
   localized in membrane domains to continuous distribution along the cell
   membrane. Our results suggest that eATP, by upregulating NTPDase2 and eN
   and altering the enzyme membrane topology, affects local kinetics of ATP
   metabolism and signal transduction that may have important roles in the
   process related to inflammation and reactive gliosis.
T2  - Journal of Molecular Neuroscience
T1  - Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
 Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
 In Vitro
IS  - 3
VL  - 57
DO  - 10.1007/s12031-015-0601-y
SP  - 452
EP  - 462
ER  - 

@article{
author = "Brisevac, Dusica and Adzic, Marija and Laketa, Danijela and Parabucki, Ana and Milosevic, Milena and Lavrnja, Irena and Bjelobaba, Ivana and Sevigny, Jean and Kipp, Markus and Nedeljkovic, Nadezda",
year = "2015",
abstract = "Extracellular ATP (eATP) acts as a danger-associated molecular pattern
   which induces reactive response of astrocytes after brain insult,
   including morphological remodeling of astrocytes, proliferation,
   chemotaxis, and release of proinflammatory cytokines. The responses
   induced by eATP are under control of ecto-nucleotidases, which catalyze
   sequential hydrolysis of ATP to adenosine. In the mammalian brain,
   ecto-nucleotidases comprise three enzyme families: ecto-nucleoside
   triphosphate diphosphohydrolases 1-3 (NTPDase1-3), ecto-nucleotide
   pyrophosphatase/phospodiesterases 1-3 (NPP1-3), and ecto-5'-nucleotidase
   (eN), which crucially determine ATP/adenosine ratio in the pericellular
   milieu. Altered expression of ecto-nucleotidases has been demonstrated
   in several experimental models of human brain dysfunctions. In the
   present study, we have explored the pattern of NTPDase1-3, NPP1-3, and
   eN expression by cultured cortical astrocytes challenged with 1 mmol/L
   ATP (eATP). At the transcriptional level, eATP upregulated expression of
   NTPDase1, NTPDase2, NPP2, and eN, while, at translational and functional
   levels, these were paralleled only by the induction of NTPDase2 and eN.
   Additionally, eATP altered membrane topology of eN, from clusters
   localized in membrane domains to continuous distribution along the cell
   membrane. Our results suggest that eATP, by upregulating NTPDase2 and eN
   and altering the enzyme membrane topology, affects local kinetics of ATP
   metabolism and signal transduction that may have important roles in the
   process related to inflammation and reactive gliosis.",
journal = "Journal of Molecular Neuroscience",
title = "Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
 Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
 In Vitro",
number = "3",
volume = "57",
doi = "10.1007/s12031-015-0601-y",
pages = "452-462"
}

Brisevac, D., Adzic, M., Laketa, D., Parabucki, A., Milosevic, M., Lavrnja, I., Bjelobaba, I., Sevigny, J., Kipp, M.,& Nedeljkovic, N.. (2015). Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
 Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
 In Vitro. in Journal of Molecular Neuroscience, 57(3), 452-462.
https://doi.org/10.1007/s12031-015-0601-y

Brisevac D, Adzic M, Laketa D, Parabucki A, Milosevic M, Lavrnja I, Bjelobaba I, Sevigny J, Kipp M, Nedeljkovic N. Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
 Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
 In Vitro. in Journal of Molecular Neuroscience. 2015;57(3):452-462.
doi:10.1007/s12031-015-0601-y .

Brisevac, Dusica, Adzic, Marija, Laketa, Danijela, Parabucki, Ana, Milosevic, Milena, Lavrnja, Irena, Bjelobaba, Ivana, Sevigny, Jean, Kipp, Markus, Nedeljkovic, Nadezda, "Extracellular ATP Selectively Upregulates Ecto-Nucleoside Triphosphate
 Diphosphohydrolase 2 and Ecto-5'-Nucleotidase by Rat Cortical Astrocytes
 In Vitro" in Journal of Molecular Neuroscience, 57, no. 3 (2015):452-462,
https://doi.org/10.1007/s12031-015-0601-y . .