TY  - JOUR
AU  - Laketa, Danijela
AU  - Savić, Jasmina
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Vasić, Vesna
AU  - Stojiljković, Mirjana
AU  - Nedeljković, Nadežda
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2093
AB  - Background: Cortical stab injury (CSI) induces changes in the activity,
   expression and cellular distribution of specific ectonucleotidases at
   the injury site. Also, several experimentally induced neuropathologies
   are associated with changes in soluble ectonucleotidase activities in
   the plasma and serum, whilst various insults to the brain alter purine
   compounds levels in cerebrospinal fluid, but also in serum, indicating
   that insults to the brain may induce alterations in nucleotides release
   and rate of their hydrolysis in the vascular system. Since adenine
   nucleotides and adenosine regulate diverse cellular functions in the
   vascular system, including vascular tone, platelet aggregation and
   inflammatory responses of lymphocytes and macrophages, alterations of
   ectonucleotidase activities in the vascular system may be relevant for
   the clinical outcome of the primary insult.
   Methods: We explored ectonucleotidase activities using specific enzyme
   assays and determined adenine nucleotides concentrations by the UPLC
   method in the rat serum after cortical stab injury.
   Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
   60\% and 40\%, respectively, while phosphodiesterase activity remained
   unchanged. Also, at 4-h postinjury a marked decrease in ATP
   concentration and more than 2-fold increase in AMP concentration were
   recorded.
   Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
   soluble ectonucleotidases in rat serum, which leads to the observed
   shift in serum nucleotide levels. The results obtained imply that
   ectonucleotidases and adenine nucleotides participate in the
   communication between the brain and the vascular system in physiological
   and pathological conditions and thereby may be involved in the
   development of various human neuropathologies.
T2  - Journal of Medical Biochemistry
T1  - BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM
IS  - 2
VL  - 34
DO  - 10.2478/jomb-2014-0025
SP  - 215
EP  - 222
ER  - 

@article{
author = "Laketa, Danijela and Savić, Jasmina and Bjelobaba, Ivana and Lavrnja, Irena and Vasić, Vesna and Stojiljković, Mirjana and Nedeljković, Nadežda",
year = "2015",
abstract = "Background: Cortical stab injury (CSI) induces changes in the activity,
   expression and cellular distribution of specific ectonucleotidases at
   the injury site. Also, several experimentally induced neuropathologies
   are associated with changes in soluble ectonucleotidase activities in
   the plasma and serum, whilst various insults to the brain alter purine
   compounds levels in cerebrospinal fluid, but also in serum, indicating
   that insults to the brain may induce alterations in nucleotides release
   and rate of their hydrolysis in the vascular system. Since adenine
   nucleotides and adenosine regulate diverse cellular functions in the
   vascular system, including vascular tone, platelet aggregation and
   inflammatory responses of lymphocytes and macrophages, alterations of
   ectonucleotidase activities in the vascular system may be relevant for
   the clinical outcome of the primary insult.
   Methods: We explored ectonucleotidase activities using specific enzyme
   assays and determined adenine nucleotides concentrations by the UPLC
   method in the rat serum after cortical stab injury.
   Results: At 4-h post-injury, ATP and AMP hydrolysis increased by about
   60\% and 40\%, respectively, while phosphodiesterase activity remained
   unchanged. Also, at 4-h postinjury a marked decrease in ATP
   concentration and more than 2-fold increase in AMP concentration were
   recorded.
   Conclusions: CSI induces rapid up-regulation of nucleotide catabolizing
   soluble ectonucleotidases in rat serum, which leads to the observed
   shift in serum nucleotide levels. The results obtained imply that
   ectonucleotidases and adenine nucleotides participate in the
   communication between the brain and the vascular system in physiological
   and pathological conditions and thereby may be involved in the
   development of various human neuropathologies.",
journal = "Journal of Medical Biochemistry",
title = "BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM",
number = "2",
volume = "34",
doi = "10.2478/jomb-2014-0025",
pages = "215-222"
}

Laketa, D., Savić, J., Bjelobaba, I., Lavrnja, I., Vasić, V., Stojiljković, M.,& Nedeljković, N.. (2015). BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM. in Journal of Medical Biochemistry, 34(2), 215-222.
https://doi.org/10.2478/jomb-2014-0025

Laketa D, Savić J, Bjelobaba I, Lavrnja I, Vasić V, Stojiljković M, Nedeljković N. BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM. in Journal of Medical Biochemistry. 2015;34(2):215-222.
doi:10.2478/jomb-2014-0025 .

Laketa, Danijela, Savić, Jasmina, Bjelobaba, Ivana, Lavrnja, Irena, Vasić, Vesna, Stojiljković, Mirjana, Nedeljković, Nadežda, "BRAIN INJURY ALTERS ECTONUCLEOTIDASE ACTIVITIES AND ADENINE NUCLEOTIDE
 LEVELS IN RAT SERUM" in Journal of Medical Biochemistry, 34, no. 2 (2015):215-222,
https://doi.org/10.2478/jomb-2014-0025 . .