TY  - JOUR
AU  - Stajić, Mirjana M
AU  - Glamočlija, Jasmina
AU  - Maksimović, Vuk M
AU  - Vukojević, Jelena B.
AU  - Simonić, Jasmina
AU  - Zervakis, George
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1328
AB  - Ganoderma lucidum is a well-known medicinal mushroom species in which polysaccharides are one of the major sources of biological activity. The species was considered as a species-complex due to significant variations in morphological, biochemical, and genetic features among populations with a worldwide distribution. This fact was the basis for setting the aim of this research: to study intraspecific diversity in polysaccharide production and intracellular sugar composition among selected G. lucidum strains. The presence of intraspecific diversity among 10 G. lucidum strains, from different areas worldwide, was noted. Values of produced mycelia biomass and intracellular polysaccharides were found in wide ranges (3.1-28.2 g L-1 and 20.0-53.3 mg g(-1), respectively), while differences in extracellular polysaccharide amounts were minor (0.2-1.5 mg mL(-1)). The significant quantitative and qualitative differences in intracellular sugar composition were noted. Glucose was the predominant sugar in almost all strains except one (HAI 447), where sucrose was dominant. The potential of polysaccharide production and intracellular sugar composition could be one more taxonomic criterion for strain characterization within G. lucidum. The differences in intracellular sugar composition and proportions could be reflected in features of produced polysaccharides and also in their biological activities.
T2  - International Journal of Medicinal Mushrooms
T1  - A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)
IS  - 2
VL  - 13
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1328
ER  - 

@article{
author = "Stajić, Mirjana M and Glamočlija, Jasmina and Maksimović, Vuk M and Vukojević, Jelena B. and Simonić, Jasmina and Zervakis, George",
year = "2011",
abstract = "Ganoderma lucidum is a well-known medicinal mushroom species in which polysaccharides are one of the major sources of biological activity. The species was considered as a species-complex due to significant variations in morphological, biochemical, and genetic features among populations with a worldwide distribution. This fact was the basis for setting the aim of this research: to study intraspecific diversity in polysaccharide production and intracellular sugar composition among selected G. lucidum strains. The presence of intraspecific diversity among 10 G. lucidum strains, from different areas worldwide, was noted. Values of produced mycelia biomass and intracellular polysaccharides were found in wide ranges (3.1-28.2 g L-1 and 20.0-53.3 mg g(-1), respectively), while differences in extracellular polysaccharide amounts were minor (0.2-1.5 mg mL(-1)). The significant quantitative and qualitative differences in intracellular sugar composition were noted. Glucose was the predominant sugar in almost all strains except one (HAI 447), where sucrose was dominant. The potential of polysaccharide production and intracellular sugar composition could be one more taxonomic criterion for strain characterization within G. lucidum. The differences in intracellular sugar composition and proportions could be reflected in features of produced polysaccharides and also in their biological activities.",
journal = "International Journal of Medicinal Mushrooms",
title = "A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)",
number = "2",
volume = "13",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1328"
}

Stajić, M. M., Glamočlija, J., Maksimović, V. M., Vukojević, J. B., Simonić, J.,& Zervakis, G.. (2011). A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms, 13(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1328

Stajić MM, Glamočlija J, Maksimović VM, Vukojević JB, Simonić J, Zervakis G. A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms. 2011;13(2):null-158.
https://hdl.handle.net/21.15107/rcub_ibiss_1328 .

Stajić, Mirjana M, Glamočlija, Jasmina, Maksimović, Vuk M, Vukojević, Jelena B., Simonić, Jasmina, Zervakis, George, "A Comparative Assessment of the Potential of Polysaccharide Production and Intracellular Sugar Composition within Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.)P. Karst. (Aphyllophoromycetideae)" in International Journal of Medicinal Mushrooms, 13, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1328 .

TY  - JOUR
AU  - Simonić, Jasmina
AU  - Vukojević, Jelena B.
AU  - Stajić, Mirjana M
AU  - Glamočlija, Jasmina
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1344
AB  - Comparison of the potential for laccase and Mn-oxidizing peroxidases synthesis by ten strains of Ganoderma lucidum, originating from different worldwide areas, during solid-state fermentation of selected plant raw materials was the aim of this study. The great intraspecific variability in the production of analyzed enzymes as well as the dependence of the enzyme activity on plant raw materials were reported. The strain HAI 957 was the best laccase producer in the presence of corn stem, as a unique carbon source (129.46 U/L). The highest level of Mn-dependent peroxidase activity was noted after wheat straw fermentation by G. lucidum HAI 246 (78.64 U/L), while the maximal versatile peroxidase production (59.72 U/L) was observed in strain HAI 957 in the medium with oak sawdust.
T2  - Applied Biochemistry and Biotechnology
T1  - Intraspecific Diversity within Ganoderma lucidum in the Production of Laccase and Mn-Oxidizing Peroxidases During Plant Residues Fermentation
IS  - 2
VL  - 162
EP  - 415
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1344
ER  - 

@article{
author = "Simonić, Jasmina and Vukojević, Jelena B. and Stajić, Mirjana M and Glamočlija, Jasmina",
year = "2010",
abstract = "Comparison of the potential for laccase and Mn-oxidizing peroxidases synthesis by ten strains of Ganoderma lucidum, originating from different worldwide areas, during solid-state fermentation of selected plant raw materials was the aim of this study. The great intraspecific variability in the production of analyzed enzymes as well as the dependence of the enzyme activity on plant raw materials were reported. The strain HAI 957 was the best laccase producer in the presence of corn stem, as a unique carbon source (129.46 U/L). The highest level of Mn-dependent peroxidase activity was noted after wheat straw fermentation by G. lucidum HAI 246 (78.64 U/L), while the maximal versatile peroxidase production (59.72 U/L) was observed in strain HAI 957 in the medium with oak sawdust.",
journal = "Applied Biochemistry and Biotechnology",
title = "Intraspecific Diversity within Ganoderma lucidum in the Production of Laccase and Mn-Oxidizing Peroxidases During Plant Residues Fermentation",
number = "2",
volume = "162",
pages = "415",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1344"
}

Simonić, J., Vukojević, J. B., Stajić, M. M.,& Glamočlija, J.. (2010). Intraspecific Diversity within Ganoderma lucidum in the Production of Laccase and Mn-Oxidizing Peroxidases During Plant Residues Fermentation. in Applied Biochemistry and Biotechnology, 162(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1344

Simonić J, Vukojević JB, Stajić MM, Glamočlija J. Intraspecific Diversity within Ganoderma lucidum in the Production of Laccase and Mn-Oxidizing Peroxidases During Plant Residues Fermentation. in Applied Biochemistry and Biotechnology. 2010;162(2):null-415.
https://hdl.handle.net/21.15107/rcub_ibiss_1344 .

Simonić, Jasmina, Vukojević, Jelena B., Stajić, Mirjana M, Glamočlija, Jasmina, "Intraspecific Diversity within Ganoderma lucidum in the Production of Laccase and Mn-Oxidizing Peroxidases During Plant Residues Fermentation" in Applied Biochemistry and Biotechnology, 162, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1344 .

TY  - JOUR
AU  - Simonić, Jasmina
AU  - Stajić, Mirjana M
AU  - Kukavica, Biljana M
AU  - Vukojević, Jelena B.
AU  - Veljović-Jovanović, Sonja D
AU  - Duletić-Laušević, Sonja N.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1407
AB  - The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH(4)NO(3) was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.
T2  - Bioresources
T1  - Wheat Straw Conversion By Enzymatic System of Ganoderma Lucidum
IS  - 4
VL  - 5
EP  - 2373
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1407
ER  - 

@article{
author = "Simonić, Jasmina and Stajić, Mirjana M and Kukavica, Biljana M and Vukojević, Jelena B. and Veljović-Jovanović, Sonja D and Duletić-Laušević, Sonja N.",
year = "2010",
abstract = "The purpose of this study was to resolve the question of whether various nitrogen sources and concentrations affect characteristics of selected G. lucidum ligninolytic enzymes participating in wheat straw fermentation. This is the first study reporting the presence of versatile peroxidase activity in crude extract of G. lucidum culture, as well as isoforms profile of Mn-oxidizing peroxidases. NH(4)NO(3) was the optimum nitrogen source for laccase and Mn-dependent peroxidase activity, while peptone was the optimum one for versatile peroxidase activity. Four bands with laccase activity were obtained by native PAGE and IEF separations from medium enriched with inorganic nitrogen source, and only two bands from medium containing organic source. Medium composition was not shown to affect isoenzyme patterns of Mn-oxidizing peroxidases. Four isoforms of Mn-dependent peroxidase and three of versatile peroxidase were obtained on native PAGE. By IEF separation, five isoforms of Mn-dependent peroxidase and only two of versatile peroxidase were observed. The results demonstrated that G. lucidum has potential for mineralization and transformation of various agricultural residues and should take more significant participation in large-scale biotechnological processes.",
journal = "Bioresources",
title = "Wheat Straw Conversion By Enzymatic System of Ganoderma Lucidum",
number = "4",
volume = "5",
pages = "2373",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1407"
}

Simonić, J., Stajić, M. M., Kukavica, B. M., Vukojević, J. B., Veljović-Jovanović, S. D.,& Duletić-Laušević, S. N.. (2010). Wheat Straw Conversion By Enzymatic System of Ganoderma Lucidum. in Bioresources, 5(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1407

Simonić J, Stajić MM, Kukavica BM, Vukojević JB, Veljović-Jovanović SD, Duletić-Laušević SN. Wheat Straw Conversion By Enzymatic System of Ganoderma Lucidum. in Bioresources. 2010;5(4):null-2373.
https://hdl.handle.net/21.15107/rcub_ibiss_1407 .

Simonić, Jasmina, Stajić, Mirjana M, Kukavica, Biljana M, Vukojević, Jelena B., Veljović-Jovanović, Sonja D, Duletić-Laušević, Sonja N., "Wheat Straw Conversion By Enzymatic System of Ganoderma Lucidum" in Bioresources, 5, no. 4 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1407 .

TY  - JOUR
AU  - Simonić, Jasmina
AU  - Stajić, Mirjana M
AU  - Glamočlija, Jasmina
AU  - Vukojević, Jelena B.
AU  - Duletić-Laušević, Sonja N.
AU  - Brceski, Ilija D
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1559
AB  - This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.
T2  - International Journal of Medicinal Mushrooms
T1  - Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
IS  - 4
VL  - 10
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1559
ER  - 

@article{
author = "Simonić, Jasmina and Stajić, Mirjana M and Glamočlija, Jasmina and Vukojević, Jelena B. and Duletić-Laušević, Sonja N. and Brceski, Ilija D",
year = "2008",
abstract = "This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.",
journal = "International Journal of Medicinal Mushrooms",
title = "Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)",
number = "4",
volume = "10",
pages = "360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1559"
}

Simonić, J., Stajić, M. M., Glamočlija, J., Vukojević, J. B., Duletić-Laušević, S. N.,& Brceski, I. D.. (2008). Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms, 10(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1559

Simonić J, Stajić MM, Glamočlija J, Vukojević JB, Duletić-Laušević SN, Brceski ID. Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms. 2008;10(4):null-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1559 .

Simonić, Jasmina, Stajić, Mirjana M, Glamočlija, Jasmina, Vukojević, Jelena B., Duletić-Laušević, Sonja N., Brceski, Ilija D, "Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)" in International Journal of Medicinal Mushrooms, 10, no. 4 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1559 .