TY  - JOUR
AU  - Mišić, Danijela
AU  - Dragičević, Milan B.
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Maksimović, Vuk M
AU  - Momčilović, Ivana
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1110
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
IS  - 13
VL  - 169
SP  - 93
EP  - 1289
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1110
ER  - 

@article{
author = "Mišić, Danijela and Dragičević, Milan B. and Šiler, Branislav and Nestorović Živković, Jasmina and Maksimović, Vuk M and Momčilović, Ivana and Nikolić, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
number = "13",
volume = "169",
pages = "93-1289",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1110"
}

Mišić, D., Dragičević, M. B., Šiler, B., Nestorović Živković, J., Maksimović, V. M., Momčilović, I.,& Nikolić, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology, 169(13), 93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110

Mišić D, Dragičević MB, Šiler B, Nestorović Živković J, Maksimović VM, Momčilović I, Nikolić M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

Mišić, Danijela, Dragičević, Milan B., Šiler, Branislav, Nestorović Živković, Jasmina, Maksimović, Vuk M, Momčilović, Ivana, Nikolić, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):93-1289,
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Simonović, Ana
AU  - Maksimović, Vuk M
AU  - Budimir, Snežana
AU  - Janošević, Dušica A.
AU  - Đuricković, Milutin S
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1204
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
IS  - 3
VL  - 108
EP  - 400
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1204
ER  - 

@article{
author = "Mišić, Danijela and Šiler, Branislav and Nestorović Živković, Jasmina and Simonović, Ana and Maksimović, Vuk M and Budimir, Snežana and Janošević, Dušica A. and Đuricković, Milutin S and Nikolić, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
number = "3",
volume = "108",
pages = "400",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1204"
}

Mišić, D., Šiler, B., Nestorović Živković, J., Simonović, A., Maksimović, V. M., Budimir, S., Janošević, D. A., Đuricković, M. S.,& Nikolić, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture, 108(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1204

Mišić D, Šiler B, Nestorović Živković J, Simonović A, Maksimović VM, Budimir S, Janošević DA, Đuricković MS, Nikolić M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):null-400.
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

Mišić, Danijela, Šiler, Branislav, Nestorović Živković, Jasmina, Simonović, Ana, Maksimović, Vuk M, Budimir, Snežana, Janošević, Dušica A., Đuricković, Milutin S, Nikolić, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

TY  - JOUR
AU  - Stošić, Jelena
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Nikolić, Miroslav
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1355
AB  - Objective To examine the presence of gender differences in pulmonary inflammation evoked by acute systemic cadmium administration in rats. Methods Presence of basic indicators of lung inflammation (inflammatory cytokine lung content, leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed and compared in animals of the two sexes. Results Intraperitoneal administration of cadmium (1.0 mg/kg) resulted in higher cadmium content in lungs of female rats. Higher tumor necrosis factor (TNF) content was noted in lung homogenates of male rats, while interleukin-6 (IL-6) content was slightly, but signifaicantly greater in lungs of female rats. Increased leukocyte infiltration was observed in lungs of male rats, mainly due to neutrophils. Increased responsiveness to phorbol myristate acetate (PMA) stimulation was noted in cells recovered from lungs of male rats. Rise in intracellular content of myeloperoxidase (MPO) was noted in lung cells from cadmium-treated rats of both sexes, but higher in cells from male rats. Conclusion Presented data documented a more intense pulmonary inflammatory response to systemic cadmium administration in males, with higher IL-6 levels in lungs of female individuals. These sex differences in proinflamatory activity of cadmium in lungs should be taken into consideration in studying the remote toxicity of this heavy metal.
T2  - Biomedical and Environmental Sciences
T1  - Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats
IS  - 4
VL  - 23
EP  - 299
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1355
ER  - 

@article{
author = "Stošić, Jelena and Mirkov, Ivana and Belij, Sandra and Nikolić, Miroslav and Popov Aleksandrov, Aleksandra and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2010",
abstract = "Objective To examine the presence of gender differences in pulmonary inflammation evoked by acute systemic cadmium administration in rats. Methods Presence of basic indicators of lung inflammation (inflammatory cytokine lung content, leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed and compared in animals of the two sexes. Results Intraperitoneal administration of cadmium (1.0 mg/kg) resulted in higher cadmium content in lungs of female rats. Higher tumor necrosis factor (TNF) content was noted in lung homogenates of male rats, while interleukin-6 (IL-6) content was slightly, but signifaicantly greater in lungs of female rats. Increased leukocyte infiltration was observed in lungs of male rats, mainly due to neutrophils. Increased responsiveness to phorbol myristate acetate (PMA) stimulation was noted in cells recovered from lungs of male rats. Rise in intracellular content of myeloperoxidase (MPO) was noted in lung cells from cadmium-treated rats of both sexes, but higher in cells from male rats. Conclusion Presented data documented a more intense pulmonary inflammatory response to systemic cadmium administration in males, with higher IL-6 levels in lungs of female individuals. These sex differences in proinflamatory activity of cadmium in lungs should be taken into consideration in studying the remote toxicity of this heavy metal.",
journal = "Biomedical and Environmental Sciences",
title = "Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats",
number = "4",
volume = "23",
pages = "299",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1355"
}

Stošić, J., Mirkov, I., Belij, S., Nikolić, M., Popov Aleksandrov, A., Kataranovski, D. S.,& Kataranovski, M.. (2010). Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats. in Biomedical and Environmental Sciences, 23(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1355

Stošić J, Mirkov I, Belij S, Nikolić M, Popov Aleksandrov A, Kataranovski DS, Kataranovski M. Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats. in Biomedical and Environmental Sciences. 2010;23(4):null-299.
https://hdl.handle.net/21.15107/rcub_ibiss_1355 .

Stošić, Jelena, Mirkov, Ivana, Belij, Sandra, Nikolić, Miroslav, Popov Aleksandrov, Aleksandra, Kataranovski, Dragan S., Kataranovski, Milena, "Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats" in Biomedical and Environmental Sciences, 23, no. 4 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1355 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Nikolić, Miroslav
AU  - Zolotarevski, Lidija D
AU  - Ćirić, Danica
AU  - Kataranovski, Dragan S.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1433
AB  - Pulmonary inflammation is a biological response to cadmium entering the body via the respiratory route. Systemic administration of this metal revealed the lungs as a significant site of its disposition. In this study, the presence of basic indicators of lung inflammation (leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed in the rat model of acute systemic cadmium intoxication. Intraperitoneal administration of both cadmium doses (0.5 mg/kg and 1.0 mg/kg) resulted in increased numbers of neutrophils. Signs of spontaneous activation of lung cells including the capacity of reduction of nitroblue tetrazolium (NBT), increase in myeloperoxidase (MPO) intracellular content and increase in interleukin-6 (IL-6) production were noted at both cadmium doses. Increased lung cell responsiveness to stimulation in vitro was noted at the higher cadmium dose. The presence of pulmonary inflammatory parameters in rats administered intraperitoneally with cadmium revealed the lungs as remote inflammatory targets of this metal. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Environmental Toxicology and Pharmacology
T1  - Lungs: Remote inflammatory target of systemic cadmium administration in rats
IS  - 2
VL  - 28
EP  - 231
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1433
ER  - 

@article{
author = "Kataranovski, Milena and Mirkov, Ivana and Belij, Sandra and Nikolić, Miroslav and Zolotarevski, Lidija D and Ćirić, Danica and Kataranovski, Dragan S.",
year = "2009",
abstract = "Pulmonary inflammation is a biological response to cadmium entering the body via the respiratory route. Systemic administration of this metal revealed the lungs as a significant site of its disposition. In this study, the presence of basic indicators of lung inflammation (leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed in the rat model of acute systemic cadmium intoxication. Intraperitoneal administration of both cadmium doses (0.5 mg/kg and 1.0 mg/kg) resulted in increased numbers of neutrophils. Signs of spontaneous activation of lung cells including the capacity of reduction of nitroblue tetrazolium (NBT), increase in myeloperoxidase (MPO) intracellular content and increase in interleukin-6 (IL-6) production were noted at both cadmium doses. Increased lung cell responsiveness to stimulation in vitro was noted at the higher cadmium dose. The presence of pulmonary inflammatory parameters in rats administered intraperitoneally with cadmium revealed the lungs as remote inflammatory targets of this metal. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Environmental Toxicology and Pharmacology",
title = "Lungs: Remote inflammatory target of systemic cadmium administration in rats",
number = "2",
volume = "28",
pages = "231",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1433"
}

Kataranovski, M., Mirkov, I., Belij, S., Nikolić, M., Zolotarevski, L. D., Ćirić, D.,& Kataranovski, D. S.. (2009). Lungs: Remote inflammatory target of systemic cadmium administration in rats. in Environmental Toxicology and Pharmacology, 28(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1433

Kataranovski M, Mirkov I, Belij S, Nikolić M, Zolotarevski LD, Ćirić D, Kataranovski DS. Lungs: Remote inflammatory target of systemic cadmium administration in rats. in Environmental Toxicology and Pharmacology. 2009;28(2):null-231.
https://hdl.handle.net/21.15107/rcub_ibiss_1433 .

Kataranovski, Milena, Mirkov, Ivana, Belij, Sandra, Nikolić, Miroslav, Zolotarevski, Lidija D, Ćirić, Danica, Kataranovski, Dragan S., "Lungs: Remote inflammatory target of systemic cadmium administration in rats" in Environmental Toxicology and Pharmacology, 28, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1433 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Giba, Zlatko
AU  - Bacić, Goran G
AU  - Nikolić, Miroslav
AU  - Grubišić, Dragoljub
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1498
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
IS  - 3
VL  - 64
EP  - 324
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1498
ER  - 

@article{
author = "Todorović, Slađana and Giba, Zlatko and Bacić, Goran G and Nikolić, Miroslav and Grubišić, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
number = "3",
volume = "64",
pages = "324",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1498"
}

Todorović, S., Giba, Z., Bacić, G. G., Nikolić, M.,& Grubišić, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany, 64(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1498

Todorović S, Giba Z, Bacić GG, Nikolić M, Grubišić D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):null-324.
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .

Todorović, Slađana, Giba, Zlatko, Bacić, Goran G, Nikolić, Miroslav, Grubišić, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .