TY  - JOUR
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
AU  - Brkljačić, Jelena
AU  - Elaković, Ivana
AU  - Manitašević-Jovanović, Sanja
AU  - Perišić, Tatjana
AU  - Dunđerski, Jadranka S.
AU  - Damjanović, Svetozar S
AU  - Knežević, Goran
AU  - Spirić, Zeljko M
AU  - Vermetten, Eric
AU  - Savić, Danka A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/999
AB  - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
IS  - null
VL  - 43
SP  - 63
EP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_999
ER  - 

@article{
author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Brkljačić, Jelena and Elaković, Ivana and Manitašević-Jovanović, Sanja and Perišić, Tatjana and Dunđerski, Jadranka S. and Damjanović, Svetozar S and Knežević, Goran and Spirić, Zeljko M and Vermetten, Eric and Savić, Danka A",
year = "2013",
abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD",
number = "null",
volume = "43",
pages = "63-245",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_999"
}

Matić, G., Vojnović-Milutinović, D., Brkljačić, J., Elaković, I., Manitašević-Jovanović, S., Perišić, T., Dunđerski, J. S., Damjanović, S. S., Knežević, G., Spirić, Z. M., Vermetten, E.,& Savić, D. A.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43(null), 63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999

Matić G, Vojnović-Milutinović D, Brkljačić J, Elaković I, Manitašević-Jovanović S, Perišić T, Dunđerski JS, Damjanović SS, Knežević G, Spirić ZM, Vermetten E, Savić DA. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2013;43(null):63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999 .

Matić, Gordana, Vojnović-Milutinović, Danijela, Brkljačić, Jelena, Elaković, Ivana, Manitašević-Jovanović, Sanja, Perišić, Tatjana, Dunđerski, Jadranka S., Damjanović, Svetozar S, Knežević, Goran, Spirić, Zeljko M, Vermetten, Eric, Savić, Danka A, "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43, no. null (2013):63-245,
https://hdl.handle.net/21.15107/rcub_ibiss_999 .

TY  - JOUR
AU  - Savić, Danka A
AU  - Knežević, Goran
AU  - Damjanović, Svetozar S
AU  - Spirić, Zeljko M
AU  - Matić, Gordana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1145
AB  - The use of the low-dose dexamethasone suppression test (DST) as a potentially discriminative marker between post-traumatic stress disorder (PTSD) and depression is still under discussion. In order to compare the influence of these psychopathologies on the DST results, we examined suppression in war-traumatized subjects with one or both of these disorders, as well as in healthy controls. Based on our previous findings, we hypothesized that subjects with any disorder would exhibit higher dexamethasone suppression than healthy controls due to traumatic experiences. This study was a part of a broader project in which simultaneous psychological and biological investigations were carried out in hospital conditions on 399 mate participants: 57 with PTSD, 28 with depression, 76 with PTSD + depression, and 238 healthy controls. Cortisol was measured in blood samples taken at 0900 h before and after administering 0.5 mg of dexamethasone (at 2300 h). Group means standard deviation of cortisol suppression were: 79.4 +/- 18.5 in the PTSD group, 80.8 +/- 11.6 in the depression group, 77.5 +/- 24.6 in the group with PTSD+depression, and 66.8 +/- 34.6 in healthy controls. The first three groups suppressed significantly more than the fourth. When the number of traumas was introduced as a covariate, the differences disappeared. The hypothesis was confirmed: in respect to DST, the examined trauma-related psychopathologies showed the same pattern: hypersuppression, due to multiple traumatic experiences. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Psychoneuroendocrinology
T1  - Is there a biological difference between trauma-related depression and PTSD? DST says 'NO'
IS  - 9
VL  - 37
SP  - 1045
EP  - 1520
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1145
ER  - 

@article{
author = "Savić, Danka A and Knežević, Goran and Damjanović, Svetozar S and Spirić, Zeljko M and Matić, Gordana",
year = "2012",
abstract = "The use of the low-dose dexamethasone suppression test (DST) as a potentially discriminative marker between post-traumatic stress disorder (PTSD) and depression is still under discussion. In order to compare the influence of these psychopathologies on the DST results, we examined suppression in war-traumatized subjects with one or both of these disorders, as well as in healthy controls. Based on our previous findings, we hypothesized that subjects with any disorder would exhibit higher dexamethasone suppression than healthy controls due to traumatic experiences. This study was a part of a broader project in which simultaneous psychological and biological investigations were carried out in hospital conditions on 399 mate participants: 57 with PTSD, 28 with depression, 76 with PTSD + depression, and 238 healthy controls. Cortisol was measured in blood samples taken at 0900 h before and after administering 0.5 mg of dexamethasone (at 2300 h). Group means standard deviation of cortisol suppression were: 79.4 +/- 18.5 in the PTSD group, 80.8 +/- 11.6 in the depression group, 77.5 +/- 24.6 in the group with PTSD+depression, and 66.8 +/- 34.6 in healthy controls. The first three groups suppressed significantly more than the fourth. When the number of traumas was introduced as a covariate, the differences disappeared. The hypothesis was confirmed: in respect to DST, the examined trauma-related psychopathologies showed the same pattern: hypersuppression, due to multiple traumatic experiences. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Psychoneuroendocrinology",
title = "Is there a biological difference between trauma-related depression and PTSD? DST says 'NO'",
number = "9",
volume = "37",
pages = "1045-1520",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1145"
}

Savić, D. A., Knežević, G., Damjanović, S. S., Spirić, Z. M.,& Matić, G.. (2012). Is there a biological difference between trauma-related depression and PTSD? DST says 'NO'. in Psychoneuroendocrinology, 37(9), 1045-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_1145

Savić DA, Knežević G, Damjanović SS, Spirić ZM, Matić G. Is there a biological difference between trauma-related depression and PTSD? DST says 'NO'. in Psychoneuroendocrinology. 2012;37(9):1045-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_1145 .

Savić, Danka A, Knežević, Goran, Damjanović, Svetozar S, Spirić, Zeljko M, Matić, Gordana, "Is there a biological difference between trauma-related depression and PTSD? DST says 'NO'" in Psychoneuroendocrinology, 37, no. 9 (2012):1045-1520,
https://hdl.handle.net/21.15107/rcub_ibiss_1145 .

TY  - JOUR
AU  - Savić, Danka A
AU  - Knežević, Goran
AU  - Damjanović, Svetozar S
AU  - Spirić, Zeljko M
AU  - Matić, Gordana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1169
AB  - Background: Studies of cortisol in post-traumatic stress disorder (PTSD) have yielded mixed results. We hypothesize that personality traits and traumatic experiences could be the confounders of cortisol measures and disease symptoms. Method: This study was a part of a broader project in which simultaneous psychological and biological investigations were carried out in hospital conditions on 400 male participants categorized by four groups: (A) 133 with current PTSD, (B) 66 with lifetime PTSD, (C) 102 trauma controls, and (D) 99 healthy controls (matched by age and education). Cortisol and ACTH were measured in blood samples taken hourly from 22:00 h to 09:00 h, with an additional sample at 07:30 h (resting state and morning rise). The next night, dexamethasone (0.5 mg) suppression test was performed. Results: No significant differences in basal cortisol and ACTH were found between study groups. The trait Conscientiousness, negatively modulated by Extraversion (assessed by NEO Personality Inventory-Revised) was found to correlate with cortisol (but not with ACTH). Group differences are found on suppression. Structural equation modeling shows excellent fit only when the paths (influences) from Conscientiousness to basal cortisol and from traumatic events to suppression are present. The paths connecting suppression and PTSD symptoms do not contribute. Conclusions: Two sources of differences of hypothalamo-pituitary-adrenocortical axis functioning are implied, both only indirectly connected to PTSD. It seems that basal cortisol secretion is associated more tightly with personality (introvertively modulated Conscientiousness), while the regulation by glucocorticoid receptor system is sensitized by repeated traumatic situations. (c) 2011 Elsevier Ltd. All rights reserved.
T2  - Psychoneuroendocrinology
T1  - The role of personality and traumatic events in cortisol levels - Where does PTSD fit in?
IS  - 7
VL  - 37
EP  - 947
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1169
ER  - 

@article{
author = "Savić, Danka A and Knežević, Goran and Damjanović, Svetozar S and Spirić, Zeljko M and Matić, Gordana",
year = "2012",
abstract = "Background: Studies of cortisol in post-traumatic stress disorder (PTSD) have yielded mixed results. We hypothesize that personality traits and traumatic experiences could be the confounders of cortisol measures and disease symptoms. Method: This study was a part of a broader project in which simultaneous psychological and biological investigations were carried out in hospital conditions on 400 male participants categorized by four groups: (A) 133 with current PTSD, (B) 66 with lifetime PTSD, (C) 102 trauma controls, and (D) 99 healthy controls (matched by age and education). Cortisol and ACTH were measured in blood samples taken hourly from 22:00 h to 09:00 h, with an additional sample at 07:30 h (resting state and morning rise). The next night, dexamethasone (0.5 mg) suppression test was performed. Results: No significant differences in basal cortisol and ACTH were found between study groups. The trait Conscientiousness, negatively modulated by Extraversion (assessed by NEO Personality Inventory-Revised) was found to correlate with cortisol (but not with ACTH). Group differences are found on suppression. Structural equation modeling shows excellent fit only when the paths (influences) from Conscientiousness to basal cortisol and from traumatic events to suppression are present. The paths connecting suppression and PTSD symptoms do not contribute. Conclusions: Two sources of differences of hypothalamo-pituitary-adrenocortical axis functioning are implied, both only indirectly connected to PTSD. It seems that basal cortisol secretion is associated more tightly with personality (introvertively modulated Conscientiousness), while the regulation by glucocorticoid receptor system is sensitized by repeated traumatic situations. (c) 2011 Elsevier Ltd. All rights reserved.",
journal = "Psychoneuroendocrinology",
title = "The role of personality and traumatic events in cortisol levels - Where does PTSD fit in?",
number = "7",
volume = "37",
pages = "947",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1169"
}

Savić, D. A., Knežević, G., Damjanović, S. S., Spirić, Z. M.,& Matić, G.. (2012). The role of personality and traumatic events in cortisol levels - Where does PTSD fit in?. in Psychoneuroendocrinology, 37(7).
https://hdl.handle.net/21.15107/rcub_ibiss_1169

Savić DA, Knežević G, Damjanović SS, Spirić ZM, Matić G. The role of personality and traumatic events in cortisol levels - Where does PTSD fit in?. in Psychoneuroendocrinology. 2012;37(7):null-947.
https://hdl.handle.net/21.15107/rcub_ibiss_1169 .

Savić, Danka A, Knežević, Goran, Damjanović, Svetozar S, Spirić, Zeljko M, Matić, Gordana, "The role of personality and traumatic events in cortisol levels - Where does PTSD fit in?" in Psychoneuroendocrinology, 37, no. 7 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1169 .