TY  - CONF
AU  - Koller, Michel
AU  - Dielacher, Irina
AU  - Schachner, Iris
AU  - Leopold, Melanie
AU  - Jakwerth, Sefan
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toth, Erika
AU  - Savio, Domenico
AU  - Farnleitner, Andreas
AU  - Kittinger, Clemens
AU  - Kirschner, Alexander
AU  - Zarfel, Gernot
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6593
AB  - Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large
rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the
major propagation pathways and sources of ARB in the Danube, and to compare the results with
data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube
Survey (2019) were tested for their antibiotic susceptibility. 22.24% of 1700 E. coli were resistant and
12.35% were multiresistant. 15.23 % of 696 Klebsiella spp. were resistant and 1.15% were
multiresistant. In comparison the data of 2013 and 2019 of both Enterobacteriaceae showed that the
wild type is still predominant. There were significant increases especially to fluoroquinolones and
augmentin and a significant decrease of resistances to tetracycline. However, the data of JDS4
showed a significant difference between E. coli and Klebsiella spp. regarding wild type, resistance and
multiresistance. Additionally, data of 1155 E. coli showed no seasonal trends in Austria during an
annual sampling. The comparison of E. coli from biofilms and corresponding water samples revealed
several significant differences between the compartments. In conclusion, the preliminary data of E.
coli from biofilm samples suggest that the biofilm as a stable colonization within waters reflects
especially the long term situation. Thus, the stabilizing and destabilizing effects acting on ARG within
the biofilm need to be researched in greater detail. Significant differences between the closely
related Enterobacteriaceae species seem to point out the dilemma of E. coli as a solely model
organism for environmental long term effects. Klebsiella spp. and their associated ARGs are facing a
different selective pressure when encountering the native environmental populations. Finally, the
results of the annual sampling campaign suggest that single environmental differences have a higher
impact than seasonal events.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6593
ER  - 

@conference{
author = "Koller, Michel and Dielacher, Irina and Schachner, Iris and Leopold, Melanie and Jakwerth, Sefan and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toth, Erika and Savio, Domenico and Farnleitner, Andreas and Kittinger, Clemens and Kirschner, Alexander and Zarfel, Gernot",
year = "2023",
abstract = "Human induced antibiotic resistant bacteria (ARB) are not only found in clinical surroundings: large
rivers are of great concern as regards their spreading. This ongoing study´s aim is to analyse the
major propagation pathways and sources of ARB in the Danube, and to compare the results with
data obtained in 2013. Escherichia coli and Klebsiella spp. isolated during the 4th Joint Danube
Survey (2019) were tested for their antibiotic susceptibility. 22.24% of 1700 E. coli were resistant and
12.35% were multiresistant. 15.23 % of 696 Klebsiella spp. were resistant and 1.15% were
multiresistant. In comparison the data of 2013 and 2019 of both Enterobacteriaceae showed that the
wild type is still predominant. There were significant increases especially to fluoroquinolones and
augmentin and a significant decrease of resistances to tetracycline. However, the data of JDS4
showed a significant difference between E. coli and Klebsiella spp. regarding wild type, resistance and
multiresistance. Additionally, data of 1155 E. coli showed no seasonal trends in Austria during an
annual sampling. The comparison of E. coli from biofilms and corresponding water samples revealed
several significant differences between the compartments. In conclusion, the preliminary data of E.
coli from biofilm samples suggest that the biofilm as a stable colonization within waters reflects
especially the long term situation. Thus, the stabilizing and destabilizing effects acting on ARG within
the biofilm need to be researched in greater detail. Significant differences between the closely
related Enterobacteriaceae species seem to point out the dilemma of E. coli as a solely model
organism for environmental long term effects. Klebsiella spp. and their associated ARGs are facing a
different selective pressure when encountering the native environmental populations. Finally, the
results of the annual sampling campaign suggest that single environmental differences have a higher
impact than seasonal events.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6593"
}

Koller, M., Dielacher, I., Schachner, I., Leopold, M., Jakwerth, S., Kolarević, S., Kračun-Kolarević, M., Toth, E., Savio, D., Farnleitner, A., Kittinger, C., Kirschner, A.,& Zarfel, G.. (2023). The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_6593

Koller M, Dielacher I, Schachner I, Leopold M, Jakwerth S, Kolarević S, Kračun-Kolarević M, Toth E, Savio D, Farnleitner A, Kittinger C, Kirschner A, Zarfel G. The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_6593 .

Koller, Michel, Dielacher, Irina, Schachner, Iris, Leopold, Melanie, Jakwerth, Sefan, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toth, Erika, Savio, Domenico, Farnleitner, Andreas, Kittinger, Clemens, Kirschner, Alexander, Zarfel, Gernot, "The Danube's water and biofilms: Antimicrobial resistance in the Enterobacteriaceae populations" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):24,
https://hdl.handle.net/21.15107/rcub_ibiss_6593 .