TY  - CONF
AU  - Čanak Atlagić, Jelena
AU  - Marić, Ana
AU  - Marinković, Nikola
AU  - Đuknić, Jelena
AU  - Anđus, Stefan
AU  - Ilić, Marija
AU  - Tubić, Bojana
AU  - Stojanović, Katarina
AU  - Paunović, Momir
AU  - Simonović, Predrag
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6679
AB  - The brown trout is a valuable species for recreational fishing and its diet is of great interest. It feeds on 
various aquatic invertebrates, but also consumes terrestrial prey, whose contribution is often unjustly 
underestimated. The importance of terrestrial prey in diets of two trout populations was assessed at two 
sites (April-October) with different quantity of available aquatic prey (Site 1 high, Site 2 low quantity). 
Four indices of importance were used: frequency of occurrence (FO), number of prey items (AN), weight 
(AW), and index of relative importance (IRI). Preference for terrestrial prey was also estimated based on 
the electivity index (EI), where the availability of aquatic and terrestrial prey was expressed as their 
percentage in the drift samples.
Significantly less terrestrial prey was available at Site 1 (0.39% ± 0.57%) in contrast to Site 2 (13.21% ± 
10.38%), terrestrial prey was significantly more abundant in the trout diet than in the drift samples at 
both sites (t test). The EI was positive for terrestrial prey, additionally FO showed that this prey was 
present in 36% ± 16.65% (site 1) and in 87.55% ± 8.94% individuals (site 2), indicating a high preference for 
this type of prey. The importance (IRI) of terrestrial prey was higher than expected at Site 1 (1.39% ± 
1.53%; maximum 4.7% in September) and high at Site 2 (58.6% ± 23.56%; maximum 84.67% in 
September). Terrestrial prey became more present in the drift and more important in the diet toward the 
end of the study period, while the importance of aquatic prey decreased based on all four indices at both 
sites. The correlation between the increasing importance of terrestrial prey (AN, IRI) in the diet and the 
presence of aquatic prey in the drift (%) towards the end of the season was not significant (Pearson). Thus, 
the positive trend in the importance of terrestrial prey may be due to their increasing availability and 
simultaneous decline of large prey in the aquatic community due to the emergence of adults.
Terrestrial prey is generally larger (i.e., yielding more energy) than most aquatic prey and easily captured 
due to its low mobility in the aquatic environment. In the habitat with low availability of aquatic prey, 
terrestrial prey played an important role in brown trout diet. In addition, brown trout also preferred it in 
the habitat rich in aquatic prey. This study shows that terrestrial prey is significant brown trout feeding 
resource regardless of its general availability in the environment.
PB  - European Ichthyological Society
C3  - Abstract book: 17th European Congress of Ichthyology; 2023 Sep 4-8; Prague, Czech Republic
T1  - Terrestrial prey, an important part in Salmo trutta diet or a stochastic event?
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6679
ER  - 

@conference{
author = "Čanak Atlagić, Jelena and Marić, Ana and Marinković, Nikola and Đuknić, Jelena and Anđus, Stefan and Ilić, Marija and Tubić, Bojana and Stojanović, Katarina and Paunović, Momir and Simonović, Predrag",
year = "2023",
abstract = "The brown trout is a valuable species for recreational fishing and its diet is of great interest. It feeds on 
various aquatic invertebrates, but also consumes terrestrial prey, whose contribution is often unjustly 
underestimated. The importance of terrestrial prey in diets of two trout populations was assessed at two 
sites (April-October) with different quantity of available aquatic prey (Site 1 high, Site 2 low quantity). 
Four indices of importance were used: frequency of occurrence (FO), number of prey items (AN), weight 
(AW), and index of relative importance (IRI). Preference for terrestrial prey was also estimated based on 
the electivity index (EI), where the availability of aquatic and terrestrial prey was expressed as their 
percentage in the drift samples.
Significantly less terrestrial prey was available at Site 1 (0.39% ± 0.57%) in contrast to Site 2 (13.21% ± 
10.38%), terrestrial prey was significantly more abundant in the trout diet than in the drift samples at 
both sites (t test). The EI was positive for terrestrial prey, additionally FO showed that this prey was 
present in 36% ± 16.65% (site 1) and in 87.55% ± 8.94% individuals (site 2), indicating a high preference for 
this type of prey. The importance (IRI) of terrestrial prey was higher than expected at Site 1 (1.39% ± 
1.53%; maximum 4.7% in September) and high at Site 2 (58.6% ± 23.56%; maximum 84.67% in 
September). Terrestrial prey became more present in the drift and more important in the diet toward the 
end of the study period, while the importance of aquatic prey decreased based on all four indices at both 
sites. The correlation between the increasing importance of terrestrial prey (AN, IRI) in the diet and the 
presence of aquatic prey in the drift (%) towards the end of the season was not significant (Pearson). Thus, 
the positive trend in the importance of terrestrial prey may be due to their increasing availability and 
simultaneous decline of large prey in the aquatic community due to the emergence of adults.
Terrestrial prey is generally larger (i.e., yielding more energy) than most aquatic prey and easily captured 
due to its low mobility in the aquatic environment. In the habitat with low availability of aquatic prey, 
terrestrial prey played an important role in brown trout diet. In addition, brown trout also preferred it in 
the habitat rich in aquatic prey. This study shows that terrestrial prey is significant brown trout feeding 
resource regardless of its general availability in the environment.",
publisher = "European Ichthyological Society",
journal = "Abstract book: 17th European Congress of Ichthyology; 2023 Sep 4-8; Prague, Czech Republic",
title = "Terrestrial prey, an important part in Salmo trutta diet or a stochastic event?",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6679"
}

Čanak Atlagić, J., Marić, A., Marinković, N., Đuknić, J., Anđus, S., Ilić, M., Tubić, B., Stojanović, K., Paunović, M.,& Simonović, P.. (2023). Terrestrial prey, an important part in Salmo trutta diet or a stochastic event?. in Abstract book: 17th European Congress of Ichthyology; 2023 Sep 4-8; Prague, Czech Republic
European Ichthyological Society., 104.
https://hdl.handle.net/21.15107/rcub_ibiss_6679

Čanak Atlagić J, Marić A, Marinković N, Đuknić J, Anđus S, Ilić M, Tubić B, Stojanović K, Paunović M, Simonović P. Terrestrial prey, an important part in Salmo trutta diet or a stochastic event?. in Abstract book: 17th European Congress of Ichthyology; 2023 Sep 4-8; Prague, Czech Republic. 2023;:104.
https://hdl.handle.net/21.15107/rcub_ibiss_6679 .

Čanak Atlagić, Jelena, Marić, Ana, Marinković, Nikola, Đuknić, Jelena, Anđus, Stefan, Ilić, Marija, Tubić, Bojana, Stojanović, Katarina, Paunović, Momir, Simonović, Predrag, "Terrestrial prey, an important part in Salmo trutta diet or a stochastic event?" in Abstract book: 17th European Congress of Ichthyology; 2023 Sep 4-8; Prague, Czech Republic (2023):104,
https://hdl.handle.net/21.15107/rcub_ibiss_6679 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kolarević, Stoimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6592
AB  - Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6592
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kolarević, Stoimir",
year = "2023",
abstract = "Assessment of impact of pollution in the environmental studies requires multi-endpoints approach to properly
link cause and effects of pollution with focus on chemical pollution. In this sense systematic weight-of-evidence
approach (WoE) with multiple lines of evidence (LoEs) is preferable to apply. The WoE approach highlights the
importance to identify strengths and weaknesses of used LoEs. Therefore, in this study we have tested efficacy
of genotoxicological endpoints as one of the LoEs in the in situ assessment of pollution effects in the freshwater
ecosystems using Alburnus alburnus (bleak) as a bioindicator species. Additional LoEs that were used in the
study are: component-based methods for the assessment of SumTU in water based on monitoring data of the
Serbian Environmental Protection Agency (SEPA), effect based methods employing in vitro genotoxicological
analyses of Joint Danube Survey 4 (JDS4) water extracts and field derived species inventories for the
assessment and indication of ecological status/potential based on SEPA and JDS4 data. The study was
conducted within the JDS4 campaign at nine sampling sites at the Tisa, Sava, Velika Morava and Danube rivers
in the Republic of Serbia. In the case of three sampling sites, the results were uniform, meaning that all four
LoEs pointed to pollution pressure. The differences in the LoEs outcomes for other sites indicated the
importance of multiple LoEs approach for proper identification of ecological impact. In the case of current
study we have identified comet and micronucleus assay to be appropriate for the genotoxicological assessment
in the in situ studies due to high sensitivity in discrimination of sites in relation to pollution intensity, while
RAPD analysis to be more suitable for controlled ex situ investigations.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6592"
}

Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kolarević, S.. (2023). In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592

Kračun-Kolarević M, Jovanović Marić J, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kolarević S. In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kolarević, Stoimir, "In situ genotoxicity assessment in the weight-of-evidence approach – The Joint Danube Survey 4 case study" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):18,
https://hdl.handle.net/21.15107/rcub_ibiss_6592 .

TY  - JOUR
AU  - Nikolić, Vera
AU  - Nedić, Zlatko
AU  - Škraba Jurlina, Dubravka
AU  - Đikanović, Vesna
AU  - Kanjuh, Tamara
AU  - Marić, Ana
AU  - Simonović, Predrag
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5919
AB  - Over the last 14 years, ichthyological and ecological parameters have been monitored in
the Labudovo okno Ramsar site. This area is important for its biodiversity as it is home to many
rare and endangered plants and animal species. A total of 3861 fish specimens were sampled and
measured at six sampling sites four times during the sampling period. An analysis of biodiversity
indexes, relative biomass (kg/ha), and relative annual production (kg/ha) was carried out to assess
the effectiveness of existing conservation measures. The results obtained show a trend decline in
biodiversity, relative biomass, and relative annual production. This indicates a biodiversity conservation
problem that should be addressed through other mechanisms in addition to the principles of the
Ramsar Convention.
PB  - Basel: MDPI
T2  - Sustainability
T1  - Status and Perspectives of the Ichthyofauna of the Labudovo okno Ramsar Site: An Analysis of 14 Years of Data
IS  - 12
VL  - 15
DO  - 10.3390/su15129303
SP  - 9303
ER  - 

@article{
author = "Nikolić, Vera and Nedić, Zlatko and Škraba Jurlina, Dubravka and Đikanović, Vesna and Kanjuh, Tamara and Marić, Ana and Simonović, Predrag",
year = "2023",
abstract = "Over the last 14 years, ichthyological and ecological parameters have been monitored in
the Labudovo okno Ramsar site. This area is important for its biodiversity as it is home to many
rare and endangered plants and animal species. A total of 3861 fish specimens were sampled and
measured at six sampling sites four times during the sampling period. An analysis of biodiversity
indexes, relative biomass (kg/ha), and relative annual production (kg/ha) was carried out to assess
the effectiveness of existing conservation measures. The results obtained show a trend decline in
biodiversity, relative biomass, and relative annual production. This indicates a biodiversity conservation
problem that should be addressed through other mechanisms in addition to the principles of the
Ramsar Convention.",
publisher = "Basel: MDPI",
journal = "Sustainability",
title = "Status and Perspectives of the Ichthyofauna of the Labudovo okno Ramsar Site: An Analysis of 14 Years of Data",
number = "12",
volume = "15",
doi = "10.3390/su15129303",
pages = "9303"
}

Nikolić, V., Nedić, Z., Škraba Jurlina, D., Đikanović, V., Kanjuh, T., Marić, A.,& Simonović, P.. (2023). Status and Perspectives of the Ichthyofauna of the Labudovo okno Ramsar Site: An Analysis of 14 Years of Data. in Sustainability
Basel: MDPI., 15(12), 9303.
https://doi.org/10.3390/su15129303

Nikolić V, Nedić Z, Škraba Jurlina D, Đikanović V, Kanjuh T, Marić A, Simonović P. Status and Perspectives of the Ichthyofauna of the Labudovo okno Ramsar Site: An Analysis of 14 Years of Data. in Sustainability. 2023;15(12):9303.
doi:10.3390/su15129303 .

Nikolić, Vera, Nedić, Zlatko, Škraba Jurlina, Dubravka, Đikanović, Vesna, Kanjuh, Tamara, Marić, Ana, Simonović, Predrag, "Status and Perspectives of the Ichthyofauna of the Labudovo okno Ramsar Site: An Analysis of 14 Years of Data" in Sustainability, 15, no. 12 (2023):9303,
https://doi.org/10.3390/su15129303 . .

TY  - CONF
AU  - Čanak Atlagić, Jelena
AU  - Marinković, Nikola
AU  - Marić, Ana
AU  - Tubić, Bojana
AU  - Đuknić, Jelena
AU  - Anđus, Stefan
AU  - Paunović, Momir
AU  - Simonović, Predrag
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5687
AB  - Fish production is a direct measure of habitat quality. Physical characteristics of the habitat, as well as the quality and quantity of available food, can promote or slow the fish growth. Brown trout populations were monitored from April to October (2015) at three study sites with different habitat characteristics. Brown trout growth parameters (biomass, production, overall growth quality) and environmental parameters (water conductivity, suspended particles, prey biomass, prey diversity, pH, dissolved oxygen, etc.) were correlated to examine their relationship (CCA). One of the sites (Belosavac), the most productive, had high conductivity and was rich in prey, with domination of Gammaridae, but with low prey diversity and evenness. The other two sites (Rasina and Lomnica) had higher prey diversity and evenness but significantly lower prey abundance, with one of the two sites (Lomnica) having very low water conductivity and suspended particles concentration and the lowest prey abundance. High brown trout biomass and production were found to be positively correlated with high water conductivity, amount of suspended particles, prey abundance and diversity. Overall growth quality was negatively correlated with high prey diversity and evenness found at a site with very low prey abundance. According to this study, high water conductivity, as found in calcareous streams, promotes high prey production and consequently high trout production, while high prey diversity does not positively affect trout production when its abundance is low.
PB  - Zagreb: Croatian Association of Freshwater Ecologists
C3  - Book of Abstracts: 4th Symposium on Freshwater Biology with the international participation; 2023 Apr 21; Zagreb, Croatia
T1  - Which habitat characteristics promote growth of brown trout (Salmo trutta L. 1758)?
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5687
ER  - 

@conference{
author = "Čanak Atlagić, Jelena and Marinković, Nikola and Marić, Ana and Tubić, Bojana and Đuknić, Jelena and Anđus, Stefan and Paunović, Momir and Simonović, Predrag",
year = "2023",
abstract = "Fish production is a direct measure of habitat quality. Physical characteristics of the habitat, as well as the quality and quantity of available food, can promote or slow the fish growth. Brown trout populations were monitored from April to October (2015) at three study sites with different habitat characteristics. Brown trout growth parameters (biomass, production, overall growth quality) and environmental parameters (water conductivity, suspended particles, prey biomass, prey diversity, pH, dissolved oxygen, etc.) were correlated to examine their relationship (CCA). One of the sites (Belosavac), the most productive, had high conductivity and was rich in prey, with domination of Gammaridae, but with low prey diversity and evenness. The other two sites (Rasina and Lomnica) had higher prey diversity and evenness but significantly lower prey abundance, with one of the two sites (Lomnica) having very low water conductivity and suspended particles concentration and the lowest prey abundance. High brown trout biomass and production were found to be positively correlated with high water conductivity, amount of suspended particles, prey abundance and diversity. Overall growth quality was negatively correlated with high prey diversity and evenness found at a site with very low prey abundance. According to this study, high water conductivity, as found in calcareous streams, promotes high prey production and consequently high trout production, while high prey diversity does not positively affect trout production when its abundance is low.",
publisher = "Zagreb: Croatian Association of Freshwater Ecologists",
journal = "Book of Abstracts: 4th Symposium on Freshwater Biology with the international participation; 2023 Apr 21; Zagreb, Croatia",
title = "Which habitat characteristics promote growth of brown trout (Salmo trutta L. 1758)?",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5687"
}

Čanak Atlagić, J., Marinković, N., Marić, A., Tubić, B., Đuknić, J., Anđus, S., Paunović, M.,& Simonović, P.. (2023). Which habitat characteristics promote growth of brown trout (Salmo trutta L. 1758)?. in Book of Abstracts: 4th Symposium on Freshwater Biology with the international participation; 2023 Apr 21; Zagreb, Croatia
Zagreb: Croatian Association of Freshwater Ecologists., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_5687

Čanak Atlagić J, Marinković N, Marić A, Tubić B, Đuknić J, Anđus S, Paunović M, Simonović P. Which habitat characteristics promote growth of brown trout (Salmo trutta L. 1758)?. in Book of Abstracts: 4th Symposium on Freshwater Biology with the international participation; 2023 Apr 21; Zagreb, Croatia. 2023;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_5687 .

Čanak Atlagić, Jelena, Marinković, Nikola, Marić, Ana, Tubić, Bojana, Đuknić, Jelena, Anđus, Stefan, Paunović, Momir, Simonović, Predrag, "Which habitat characteristics promote growth of brown trout (Salmo trutta L. 1758)?" in Book of Abstracts: 4th Symposium on Freshwater Biology with the international participation; 2023 Apr 21; Zagreb, Croatia (2023):38,
https://hdl.handle.net/21.15107/rcub_ibiss_5687 .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kelsey
AU  - Oswald, Peter
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - https://academic.oup.com/mutage/advance-article/doi/10.1093/mutage/geac024/6823671
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5243
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford: Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
IS  - 1
VL  - 38
DO  - 10.1093/mutage/geac024
SP  - 21
EP  - 32
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kelsey and Oswald, Peter and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford: Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024",
pages = "21-32"
}

Jovanović Marić, J., Kolarević, S., Đorđević, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford: Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kelsey, Oswald, Peter, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - JOUR
AU  - Marić, Ana
AU  - Špelić, Ivan
AU  - Radočaj, Tena
AU  - Vidović, Zoran
AU  - Kanjuh, Tamara
AU  - Vilizzi, Lorenzo
AU  - Piria, Marina
AU  - Nikolić, Vera
AU  - Škraba Jurlina, Dubravka
AU  - Mrdak, Danilo
AU  - Simonović, Predrag
PY  - 2022
UR  - https://neobiota.pensoft.net/article/82964/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5184
AB  - Salmonids are an extensively hatchery-reared group of fishes that have been introduced worldwide mainly for their high commercial and recreational value. The Balkan Peninsula (south-eastern Europe) is characterised by an outstanding salmonid diversity that has become threatened by the introduction of non-native salmonids whose potential risk of invasiveness in the region remains unknown and especially so under predicted climate change conditions. In this study, 13 extant and four horizon non-native salmonid species were screened for their risk of invasiveness in the Danube and Adriatic basins of four Balkan countries. Overall, six (35%) of the screened species were ranked as carrying a high risk of invasiveness under current climate conditions, whereas under predicted conditions of global warming, this number decreased to three (17%). Under current climate conditions, the very high risk (‘top invasive’) species were rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta ( sensu stricto ), whereas under predicted climate change, this was true only of O. mykiss . A high risk was also attributed to horizon vendace Coregonus albula and lake charr Salvelinus namaycush , and to extant Atlantic salmon Salmo salar and brook trout Salvelinus fontinalis , whose risk of invasiveness, except for S. fontinalis , decreased to medium. For the other eleven medium-risk species, the risk score decreased under predicted climate change, but still remained medium. The outcomes of this study reveal that global warming will influence salmonids and that only species with wider temperature tolerance, such as O. mykiss will likely prevail. It is anticipated that the present results may contribute to the implementation of appropriate management plans to prevent the introduction and translocation of non-native salmonids across the Balkan Peninsula. Additionally, adequate measures should be developed for aquaculture facilities to prevent escapees of non-native salmonids with a high risk of invasiveness, especially into recipient areas of high conservation value.
PB  - Sofia: Pensoft Publishers
T2  - NeoBiota
T1  - Changing climate may mitigate the invasiveness risk of non-native salmonids in the Danube and Adriatic basins of the Balkan Peninsula (south-eastern Europe)
VL  - 76
DO  - 10.3897/neobiota.76.82964
SP  - 135
EP  - 161
ER  - 

@article{
author = "Marić, Ana and Špelić, Ivan and Radočaj, Tena and Vidović, Zoran and Kanjuh, Tamara and Vilizzi, Lorenzo and Piria, Marina and Nikolić, Vera and Škraba Jurlina, Dubravka and Mrdak, Danilo and Simonović, Predrag",
year = "2022",
abstract = "Salmonids are an extensively hatchery-reared group of fishes that have been introduced worldwide mainly for their high commercial and recreational value. The Balkan Peninsula (south-eastern Europe) is characterised by an outstanding salmonid diversity that has become threatened by the introduction of non-native salmonids whose potential risk of invasiveness in the region remains unknown and especially so under predicted climate change conditions. In this study, 13 extant and four horizon non-native salmonid species were screened for their risk of invasiveness in the Danube and Adriatic basins of four Balkan countries. Overall, six (35%) of the screened species were ranked as carrying a high risk of invasiveness under current climate conditions, whereas under predicted conditions of global warming, this number decreased to three (17%). Under current climate conditions, the very high risk (‘top invasive’) species were rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta ( sensu stricto ), whereas under predicted climate change, this was true only of O. mykiss . A high risk was also attributed to horizon vendace Coregonus albula and lake charr Salvelinus namaycush , and to extant Atlantic salmon Salmo salar and brook trout Salvelinus fontinalis , whose risk of invasiveness, except for S. fontinalis , decreased to medium. For the other eleven medium-risk species, the risk score decreased under predicted climate change, but still remained medium. The outcomes of this study reveal that global warming will influence salmonids and that only species with wider temperature tolerance, such as O. mykiss will likely prevail. It is anticipated that the present results may contribute to the implementation of appropriate management plans to prevent the introduction and translocation of non-native salmonids across the Balkan Peninsula. Additionally, adequate measures should be developed for aquaculture facilities to prevent escapees of non-native salmonids with a high risk of invasiveness, especially into recipient areas of high conservation value.",
publisher = "Sofia: Pensoft Publishers",
journal = "NeoBiota",
title = "Changing climate may mitigate the invasiveness risk of non-native salmonids in the Danube and Adriatic basins of the Balkan Peninsula (south-eastern Europe)",
volume = "76",
doi = "10.3897/neobiota.76.82964",
pages = "135-161"
}

Marić, A., Špelić, I., Radočaj, T., Vidović, Z., Kanjuh, T., Vilizzi, L., Piria, M., Nikolić, V., Škraba Jurlina, D., Mrdak, D.,& Simonović, P.. (2022). Changing climate may mitigate the invasiveness risk of non-native salmonids in the Danube and Adriatic basins of the Balkan Peninsula (south-eastern Europe). in NeoBiota
Sofia: Pensoft Publishers., 76, 135-161.
https://doi.org/10.3897/neobiota.76.82964

Marić A, Špelić I, Radočaj T, Vidović Z, Kanjuh T, Vilizzi L, Piria M, Nikolić V, Škraba Jurlina D, Mrdak D, Simonović P. Changing climate may mitigate the invasiveness risk of non-native salmonids in the Danube and Adriatic basins of the Balkan Peninsula (south-eastern Europe). in NeoBiota. 2022;76:135-161.
doi:10.3897/neobiota.76.82964 .

Marić, Ana, Špelić, Ivan, Radočaj, Tena, Vidović, Zoran, Kanjuh, Tamara, Vilizzi, Lorenzo, Piria, Marina, Nikolić, Vera, Škraba Jurlina, Dubravka, Mrdak, Danilo, Simonović, Predrag, "Changing climate may mitigate the invasiveness risk of non-native salmonids in the Danube and Adriatic basins of the Balkan Peninsula (south-eastern Europe)" in NeoBiota, 76 (2022):135-161,
https://doi.org/10.3897/neobiota.76.82964 . .

TY  - JOUR
AU  - Piria, Marina
AU  - Špelić, Ivan
AU  - Velagić, Luana
AU  - Lisica, Ivana
AU  - Kanjuh, Tamara
AU  - Marić, Ana
AU  - Maguire, Ivana
AU  - Radočaj, Tena
AU  - Simonović, Predrag
PY  - 2022
UR  - https://www.mdpi.com/2410-3888/7/4/179
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5118
AB  - Brown trout of non-native lineages have been stocked into Croatian streams and rivers primarily to meet angler demand. The diet of brown trout in the Black Sea Basin of Croatia is poorly understood, and there are no studies examining feeding competition between the Atlantic (AT) and Danube (DA) lineages of brown trout and their hybrids (HY). The aim of this study was to examine the natural diet of brown trout of both lineages and their hybrids and to compare feeding overlap. Canonical correspondence analysis was used to investigate the relationships between feeding habits of fish from different streams and of different genetic origin. The differences in variation of the consumed prey items were analysed by canonical variate analysis, and diet overlap was assessed by the Schoener index. The results indicate that stocked brown trout (AT) adapt rapidly to new habitat and food, as revealed by the consumption of a wide range of available food items and competition for food and space by taking on the feeding behaviour of wild native conspecifics. Diet overlap was also detected between brown trout of the DA and AT lineages. This study highlights the need to implement control measures to preserve and protect the native diversity of this species.
PB  - Basel: MDPI
T2  - Fishes
T1  - Feeding Habits and Diet Overlap between Brown Trout Lineages from the Danube Basin of Croatia
IS  - 4
VL  - 7
DO  - 10.3390/fishes7040179
SP  - 179
ER  - 

@article{
author = "Piria, Marina and Špelić, Ivan and Velagić, Luana and Lisica, Ivana and Kanjuh, Tamara and Marić, Ana and Maguire, Ivana and Radočaj, Tena and Simonović, Predrag",
year = "2022",
abstract = "Brown trout of non-native lineages have been stocked into Croatian streams and rivers primarily to meet angler demand. The diet of brown trout in the Black Sea Basin of Croatia is poorly understood, and there are no studies examining feeding competition between the Atlantic (AT) and Danube (DA) lineages of brown trout and their hybrids (HY). The aim of this study was to examine the natural diet of brown trout of both lineages and their hybrids and to compare feeding overlap. Canonical correspondence analysis was used to investigate the relationships between feeding habits of fish from different streams and of different genetic origin. The differences in variation of the consumed prey items were analysed by canonical variate analysis, and diet overlap was assessed by the Schoener index. The results indicate that stocked brown trout (AT) adapt rapidly to new habitat and food, as revealed by the consumption of a wide range of available food items and competition for food and space by taking on the feeding behaviour of wild native conspecifics. Diet overlap was also detected between brown trout of the DA and AT lineages. This study highlights the need to implement control measures to preserve and protect the native diversity of this species.",
publisher = "Basel: MDPI",
journal = "Fishes",
title = "Feeding Habits and Diet Overlap between Brown Trout Lineages from the Danube Basin of Croatia",
number = "4",
volume = "7",
doi = "10.3390/fishes7040179",
pages = "179"
}

Piria, M., Špelić, I., Velagić, L., Lisica, I., Kanjuh, T., Marić, A., Maguire, I., Radočaj, T.,& Simonović, P.. (2022). Feeding Habits and Diet Overlap between Brown Trout Lineages from the Danube Basin of Croatia. in Fishes
Basel: MDPI., 7(4), 179.
https://doi.org/10.3390/fishes7040179

Piria M, Špelić I, Velagić L, Lisica I, Kanjuh T, Marić A, Maguire I, Radočaj T, Simonović P. Feeding Habits and Diet Overlap between Brown Trout Lineages from the Danube Basin of Croatia. in Fishes. 2022;7(4):179.
doi:10.3390/fishes7040179 .

Piria, Marina, Špelić, Ivan, Velagić, Luana, Lisica, Ivana, Kanjuh, Tamara, Marić, Ana, Maguire, Ivana, Radočaj, Tena, Simonović, Predrag, "Feeding Habits and Diet Overlap between Brown Trout Lineages from the Danube Basin of Croatia" in Fishes, 7, no. 4 (2022):179,
https://doi.org/10.3390/fishes7040179 . .

TY  - JOUR
AU  - Simonović, Predrag
AU  - Ristić, Ratko
AU  - Milčanović, Vukašin
AU  - Polovina, Siniša
AU  - Malušević, Ivan
AU  - Radić, Boris
AU  - Kanjuh, Tamara
AU  - Marić, Ana
AU  - Nikolić, Vera
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1002/rra.3795
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4212
AB  - Small run-of-river hydropower plants (RRHPs) have revealed strong harmful effects worldwide on stream habitats (e.g., fragmentation and destruction) and decreased fish species, especially anadromous species abundance. Recently, RRHPs have rapidly been installed in Serbia. Most were installed on montane streams because their steep slopes are most convenient for hydropower energy production at minimal costs. Brown trout Salmo trutta are prominent in the fish communities of this rarest type of aquatic ecosystems. Their native molecular diversity reflects a biodiversity hotspot in the Balkans, and they provide attractive fishing opportunities. Records from fishery management plans for highland stream fisheries where RRHPs were installed revealed reduced brown trout biomass compared with streams without RRHPs. Research on six streams with operational RRHPs revealed severe deterioration of habitat, for example, increased water temperature, reduced dissolved oxygen, and increased nutrient contents. Effects on fish communities were evident from a change in their structure. Resident, stream-dwelling brown trout were the most affected species with steep declines in abundance, biomass, and productivity. Fragmentation owing to damming was also reflected by deterioration of their age structure. Unique native brown trout stocks susceptible to adverse effects are difficult to restore by stocking, and fish passages commonly supplied as compensation to overcome the disruption of habitats cannot mitigate the threat posed to the fish.
PB  - John Wiley and Sons Ltd
T2  - River Research and Applications
T1  - Effects of run‐of‐river hydropower plants on fish communities in montane stream ecosystems in Serbia
DO  - 10.1002/rra.3795
SP  - rra.3795
ER  - 

@article{
author = "Simonović, Predrag and Ristić, Ratko and Milčanović, Vukašin and Polovina, Siniša and Malušević, Ivan and Radić, Boris and Kanjuh, Tamara and Marić, Ana and Nikolić, Vera",
year = "2021",
abstract = "Small run-of-river hydropower plants (RRHPs) have revealed strong harmful effects worldwide on stream habitats (e.g., fragmentation and destruction) and decreased fish species, especially anadromous species abundance. Recently, RRHPs have rapidly been installed in Serbia. Most were installed on montane streams because their steep slopes are most convenient for hydropower energy production at minimal costs. Brown trout Salmo trutta are prominent in the fish communities of this rarest type of aquatic ecosystems. Their native molecular diversity reflects a biodiversity hotspot in the Balkans, and they provide attractive fishing opportunities. Records from fishery management plans for highland stream fisheries where RRHPs were installed revealed reduced brown trout biomass compared with streams without RRHPs. Research on six streams with operational RRHPs revealed severe deterioration of habitat, for example, increased water temperature, reduced dissolved oxygen, and increased nutrient contents. Effects on fish communities were evident from a change in their structure. Resident, stream-dwelling brown trout were the most affected species with steep declines in abundance, biomass, and productivity. Fragmentation owing to damming was also reflected by deterioration of their age structure. Unique native brown trout stocks susceptible to adverse effects are difficult to restore by stocking, and fish passages commonly supplied as compensation to overcome the disruption of habitats cannot mitigate the threat posed to the fish.",
publisher = "John Wiley and Sons Ltd",
journal = "River Research and Applications",
title = "Effects of run‐of‐river hydropower plants on fish communities in montane stream ecosystems in Serbia",
doi = "10.1002/rra.3795",
pages = "rra.3795"
}

Simonović, P., Ristić, R., Milčanović, V., Polovina, S., Malušević, I., Radić, B., Kanjuh, T., Marić, A.,& Nikolić, V.. (2021). Effects of run‐of‐river hydropower plants on fish communities in montane stream ecosystems in Serbia. in River Research and Applications
John Wiley and Sons Ltd., rra.3795.
https://doi.org/10.1002/rra.3795

Simonović P, Ristić R, Milčanović V, Polovina S, Malušević I, Radić B, Kanjuh T, Marić A, Nikolić V. Effects of run‐of‐river hydropower plants on fish communities in montane stream ecosystems in Serbia. in River Research and Applications. 2021;:rra.3795.
doi:10.1002/rra.3795 .

Simonović, Predrag, Ristić, Ratko, Milčanović, Vukašin, Polovina, Siniša, Malušević, Ivan, Radić, Boris, Kanjuh, Tamara, Marić, Ana, Nikolić, Vera, "Effects of run‐of‐river hydropower plants on fish communities in montane stream ecosystems in Serbia" in River Research and Applications (2021):rra.3795,
https://doi.org/10.1002/rra.3795 . .

TY  - JOUR
AU  - Špelić, Ivan
AU  - Rezić, Andrea
AU  - Kanjuh, Tamara
AU  - Marić, Ana
AU  - Maguire, Ivana
AU  - Simonović, Predrag
AU  - Radočaj, Tena
AU  - Piria, Marina
PY  - 2021
UR  - https://www.kmae-journal.org/10.1051/kmae/2021021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4405
AB  - Brown trout is a salmonid fish with a natural range extending throughout western Eurasia and North Africa. Due to its commercial value, it has also been introduced worldwide. In continental Croatia, introduced trout of the Atlantic lineage hybridizes with native trout of the Danubian lineage, threatening the native genetic diversity. The geometric morphometrics approach was used in this study to analyse changes in shape between native trout, introduced trout and their hybrids, classified a priori by molecular phylogenetic analyses. A total of 19 landmarks and semi-landmarks were used to capture the shape of 92 trout individuals belonging to two lineages and their hybrids. Canonical variate analysis and discriminant function analysis were used to analyse and describe shape variation. A significant difference was found between the shape of the Atlantic lineage trout and both Danubian lineage trout and hybrids, with the most prominent differences in body depth, head length and eye size. No statistically significant shape differences were observed between Danubian lineage trout and the hybrids. The observed significant differences in shape could be the result of genetic diversity or trout phenotypic plasticity. Further studies are needed to clarify the origin of this variation in shape.
T2  - Knowledge & Management of Aquatic Ecosystems
T1  - Application of the geometric morphometrics approach in the discrimination of morphological traits between brown trout lineages in the Danube Basin of Croatia
IS  - 422
VL  - 2021
DO  - 10.1051/kmae/2021021
SP  - 22
ER  - 

@article{
author = "Špelić, Ivan and Rezić, Andrea and Kanjuh, Tamara and Marić, Ana and Maguire, Ivana and Simonović, Predrag and Radočaj, Tena and Piria, Marina",
year = "2021",
abstract = "Brown trout is a salmonid fish with a natural range extending throughout western Eurasia and North Africa. Due to its commercial value, it has also been introduced worldwide. In continental Croatia, introduced trout of the Atlantic lineage hybridizes with native trout of the Danubian lineage, threatening the native genetic diversity. The geometric morphometrics approach was used in this study to analyse changes in shape between native trout, introduced trout and their hybrids, classified a priori by molecular phylogenetic analyses. A total of 19 landmarks and semi-landmarks were used to capture the shape of 92 trout individuals belonging to two lineages and their hybrids. Canonical variate analysis and discriminant function analysis were used to analyse and describe shape variation. A significant difference was found between the shape of the Atlantic lineage trout and both Danubian lineage trout and hybrids, with the most prominent differences in body depth, head length and eye size. No statistically significant shape differences were observed between Danubian lineage trout and the hybrids. The observed significant differences in shape could be the result of genetic diversity or trout phenotypic plasticity. Further studies are needed to clarify the origin of this variation in shape.",
journal = "Knowledge & Management of Aquatic Ecosystems",
title = "Application of the geometric morphometrics approach in the discrimination of morphological traits between brown trout lineages in the Danube Basin of Croatia",
number = "422",
volume = "2021",
doi = "10.1051/kmae/2021021",
pages = "22"
}

Špelić, I., Rezić, A., Kanjuh, T., Marić, A., Maguire, I., Simonović, P., Radočaj, T.,& Piria, M.. (2021). Application of the geometric morphometrics approach in the discrimination of morphological traits between brown trout lineages in the Danube Basin of Croatia. in Knowledge & Management of Aquatic Ecosystems, 2021(422), 22.
https://doi.org/10.1051/kmae/2021021

Špelić I, Rezić A, Kanjuh T, Marić A, Maguire I, Simonović P, Radočaj T, Piria M. Application of the geometric morphometrics approach in the discrimination of morphological traits between brown trout lineages in the Danube Basin of Croatia. in Knowledge & Management of Aquatic Ecosystems. 2021;2021(422):22.
doi:10.1051/kmae/2021021 .

Špelić, Ivan, Rezić, Andrea, Kanjuh, Tamara, Marić, Ana, Maguire, Ivana, Simonović, Predrag, Radočaj, Tena, Piria, Marina, "Application of the geometric morphometrics approach in the discrimination of morphological traits between brown trout lineages in the Danube Basin of Croatia" in Knowledge & Management of Aquatic Ecosystems, 2021, no. 422 (2021):22,
https://doi.org/10.1051/kmae/2021021 . .

TY  - JOUR
AU  - Čanak Atlagić, Jelena
AU  - Marić, Ana
AU  - Tubić, Bojana
AU  - Anđus, Stefan
AU  - Đuknić, Jelena
AU  - Marković, Vanja
AU  - Paunović, Momir
AU  - Simonović, Predrag
PY  - 2021
UR  - https://www.mdpi.com/2073-4441/13/18/2492
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4485
AB  - Examination of brown trout seasonal diet variation and investigation of terrestrial prey importance in a food-rich stream using four indices of prey importance (number and weight abundance, frequency of occurrence, index of relative importance) revealed that aquatic prey constituted the major part of the diet (>90%) throughout the examined period. Despite Gammaridae being the most abundant in the environment, other less abundant organisms appeared to be important prey, including terrestrial organisms, with maximum consumption in September. The electivity index showed a positive selection of rare prey types; Tokeshi’s model revealed a specialist strategy for most of the population, except for those of 1+ age, who were inclining to generalist strategy. Diet diversity increased throughout April to October, and ages 1+ and 2+ exhibited a more diverse diet than older ages. Diet overlap between age classes was considerable, with less overlap observed in the later season. This pattern of differentiation in the diet of brown trout age classes and their feeding plasticity over seasonal scales, as observed in this food-rich stream, provides a starting point for further examination of this topic in streams with similar or different food richness and availability.
PB  - Basel: MDPI
T2  - Water (Switzerland)
T1  - What’s on the menu for the resident brown trout in a rich limestone stream?
IS  - 18
VL  - 13
DO  - 10.3390/w13182492
SP  - 2492
ER  - 

@article{
author = "Čanak Atlagić, Jelena and Marić, Ana and Tubić, Bojana and Anđus, Stefan and Đuknić, Jelena and Marković, Vanja and Paunović, Momir and Simonović, Predrag",
year = "2021",
abstract = "Examination of brown trout seasonal diet variation and investigation of terrestrial prey importance in a food-rich stream using four indices of prey importance (number and weight abundance, frequency of occurrence, index of relative importance) revealed that aquatic prey constituted the major part of the diet (>90%) throughout the examined period. Despite Gammaridae being the most abundant in the environment, other less abundant organisms appeared to be important prey, including terrestrial organisms, with maximum consumption in September. The electivity index showed a positive selection of rare prey types; Tokeshi’s model revealed a specialist strategy for most of the population, except for those of 1+ age, who were inclining to generalist strategy. Diet diversity increased throughout April to October, and ages 1+ and 2+ exhibited a more diverse diet than older ages. Diet overlap between age classes was considerable, with less overlap observed in the later season. This pattern of differentiation in the diet of brown trout age classes and their feeding plasticity over seasonal scales, as observed in this food-rich stream, provides a starting point for further examination of this topic in streams with similar or different food richness and availability.",
publisher = "Basel: MDPI",
journal = "Water (Switzerland)",
title = "What’s on the menu for the resident brown trout in a rich limestone stream?",
number = "18",
volume = "13",
doi = "10.3390/w13182492",
pages = "2492"
}

Čanak Atlagić, J., Marić, A., Tubić, B., Anđus, S., Đuknić, J., Marković, V., Paunović, M.,& Simonović, P.. (2021). What’s on the menu for the resident brown trout in a rich limestone stream?. in Water (Switzerland)
Basel: MDPI., 13(18), 2492.
https://doi.org/10.3390/w13182492

Čanak Atlagić J, Marić A, Tubić B, Anđus S, Đuknić J, Marković V, Paunović M, Simonović P. What’s on the menu for the resident brown trout in a rich limestone stream?. in Water (Switzerland). 2021;13(18):2492.
doi:10.3390/w13182492 .

Čanak Atlagić, Jelena, Marić, Ana, Tubić, Bojana, Anđus, Stefan, Đuknić, Jelena, Marković, Vanja, Paunović, Momir, Simonović, Predrag, "What’s on the menu for the resident brown trout in a rich limestone stream?" in Water (Switzerland), 13, no. 18 (2021):2492,
https://doi.org/10.3390/w13182492 . .

TY  - JOUR
AU  - Kanjuh, Tamara
AU  - Tomić, Sunčica
AU  - Marić, Ana
AU  - Škraba Jurlina, Dubravka
AU  - Nikolić, Vera
AU  - Simonović, Predrag
PY  - 2021
UR  - https://acta-zoologica-bulgarica.eu/
UR  - http://www.acta-zoologica-bulgarica.eu/2021/002487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4489
AB  - Brown trout Salmo trutta and Macedonian trout Salmo macedonicus molecular diversity in the streams draining on the southern slopes of the Stara Planina Mts. was assessed from mitochondrial DNA control region (CR) extracted from fin clip samples collected during the last ten years. It revealed an occurrence of seven haplotypes from three phylogeographic lineages (Danubian, Adriatic and Atlantic) in total. Only the two haplotypes, Da1a and Da-s6, can be inferred for certain as the native ones, owing to their occurrence in the headwaters of streams hitherto void of introgression of brown trout from downstream sections, due to unsurmountable waterfalls. The conservation status of brown trout that have the very common Da22 haplotype is not certain yet. They are very abundant in streams that were subject to massive stockings in the last ten years, occurring both in the headwaters and in the downstream sections, without the physical barrier between them. The rest of haplotypes belong either to the Atlantic or Adriatic haplogroups and they are most likely non-native in this area.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research — Bulgarian Academy of Sciences
T2  - Acta Zoologica Bulgarica
T1  - Trout Salmo spp. (Salmoniformes: Salmonidae) Molecular Diversity in Streams on the Southern Slopes of the Stara Planina Mts. in Serbia
IS  - 3
VL  - 73
SP  - 425
EP  - 429
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4489
ER  - 

@article{
author = "Kanjuh, Tamara and Tomić, Sunčica and Marić, Ana and Škraba Jurlina, Dubravka and Nikolić, Vera and Simonović, Predrag",
year = "2021",
abstract = "Brown trout Salmo trutta and Macedonian trout Salmo macedonicus molecular diversity in the streams draining on the southern slopes of the Stara Planina Mts. was assessed from mitochondrial DNA control region (CR) extracted from fin clip samples collected during the last ten years. It revealed an occurrence of seven haplotypes from three phylogeographic lineages (Danubian, Adriatic and Atlantic) in total. Only the two haplotypes, Da1a and Da-s6, can be inferred for certain as the native ones, owing to their occurrence in the headwaters of streams hitherto void of introgression of brown trout from downstream sections, due to unsurmountable waterfalls. The conservation status of brown trout that have the very common Da22 haplotype is not certain yet. They are very abundant in streams that were subject to massive stockings in the last ten years, occurring both in the headwaters and in the downstream sections, without the physical barrier between them. The rest of haplotypes belong either to the Atlantic or Adriatic haplogroups and they are most likely non-native in this area.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research — Bulgarian Academy of Sciences",
journal = "Acta Zoologica Bulgarica",
title = "Trout Salmo spp. (Salmoniformes: Salmonidae) Molecular Diversity in Streams on the Southern Slopes of the Stara Planina Mts. in Serbia",
number = "3",
volume = "73",
pages = "425-429",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4489"
}

Kanjuh, T., Tomić, S., Marić, A., Škraba Jurlina, D., Nikolić, V.,& Simonović, P.. (2021). Trout Salmo spp. (Salmoniformes: Salmonidae) Molecular Diversity in Streams on the Southern Slopes of the Stara Planina Mts. in Serbia. in Acta Zoologica Bulgarica
Sofia: Institute of Biodiversity and Ecosystem Research — Bulgarian Academy of Sciences., 73(3), 425-429.
https://hdl.handle.net/21.15107/rcub_ibiss_4489

Kanjuh T, Tomić S, Marić A, Škraba Jurlina D, Nikolić V, Simonović P. Trout Salmo spp. (Salmoniformes: Salmonidae) Molecular Diversity in Streams on the Southern Slopes of the Stara Planina Mts. in Serbia. in Acta Zoologica Bulgarica. 2021;73(3):425-429.
https://hdl.handle.net/21.15107/rcub_ibiss_4489 .

Kanjuh, Tamara, Tomić, Sunčica, Marić, Ana, Škraba Jurlina, Dubravka, Nikolić, Vera, Simonović, Predrag, "Trout Salmo spp. (Salmoniformes: Salmonidae) Molecular Diversity in Streams on the Southern Slopes of the Stara Planina Mts. in Serbia" in Acta Zoologica Bulgarica, 73, no. 3 (2021):425-429,
https://hdl.handle.net/21.15107/rcub_ibiss_4489 .

TY  - JOUR
AU  - Kanjuh, Tamara
AU  - Marić, Ana
AU  - Piria, Marina
AU  - Špelić, Ivan
AU  - Maguire, Ivana
AU  - Simonović, Predrag
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3890
AB  - Background. The molecular diversity of brown trout, Salmo trutta Linnaeus, 1758, has been poorly studied in Croatia. The control region of mitochondrial DNA (CR mtDNA) is in addition to other molecular markers a reliable for identifying phylogenetic lineages (haplogroups) and haplotypes of brown trout. Based on analyses of the control region of mitochondrial DNA several major brown trout phylogenetic lineages were identified of which the Danubian (DA) haplotypes, though not all, are considered native to Croatian rivers belonging to the Danube basin. The introduction of allochthonous haplotypes into natural streams seriously threatens the genetic diversity of this species. Therefore, the aim of this study was to map brown trout populations inhabiting Croatian rivers of the Danube River basin and to investigate their molecular diversity and phylogeographic patterns of the established haplotypes. Materials and methods. Anal fin tissue was taken from 141 specimens of brown trout in 14 localities in the protected areas of Croatia, situated in the mountainous regions of Gorski Kotar, Žumberak, as well as Mountain Papuk in the western Slavonia. The total DNA was extracted and then the amplification of the mtDNA control region was carried out using primers Trutta-mt-F and HN20. Amplification of the 440 bp long region of the LDH-C1 gene locus was done using primers Ldhxon3F and Ldhxon4R. Amplified LDH-C* fragments were used for Restriction Fragment Length Polymorphism (RFLP) analysis using BselI restriction enzyme. Results. Analysis of the CR mtDNA revealed the presence of two phylogenetic lineages, the DA and the Atlantic (AT). Haplotypes Da1, Da2, and Da22 were recorded within the DA lineage and At1 was recorded within the AT haplogroup. Two new haplotypes were described for the first time in this study and are named Da1f and Da1g. Restriction analysis of the lactate dehydrogenase gene locus revealed a high degree of hybridization between brown trout of DA and AT haplogroups. Conclusion. The results of this study confirmed the complex molecular diversity of brown trout and the high degree of the introduction of non-native haplogroups into rivers of the Danube basin in Croatia. Conservation of native brown trout populations has become evident, as introduced allochthonous DA and AT haplogroups severely disrupt the indigenous brown trout stock.
PB  - Scientific Society of Szczecin
T2  - Acta Ichthyologica et Piscatoria
T1  - Diversity of brown trout, salmo trutta (Actinopterygii: Salmoniformes: Salmonidae), in the danube river basin of croatia revealed by mitochondrial dna
IS  - 3
VL  - 50
DO  - 10.3750/AIEP/02939
SP  - 291
EP  - 300
ER  - 

@article{
author = "Kanjuh, Tamara and Marić, Ana and Piria, Marina and Špelić, Ivan and Maguire, Ivana and Simonović, Predrag",
year = "2020",
abstract = "Background. The molecular diversity of brown trout, Salmo trutta Linnaeus, 1758, has been poorly studied in Croatia. The control region of mitochondrial DNA (CR mtDNA) is in addition to other molecular markers a reliable for identifying phylogenetic lineages (haplogroups) and haplotypes of brown trout. Based on analyses of the control region of mitochondrial DNA several major brown trout phylogenetic lineages were identified of which the Danubian (DA) haplotypes, though not all, are considered native to Croatian rivers belonging to the Danube basin. The introduction of allochthonous haplotypes into natural streams seriously threatens the genetic diversity of this species. Therefore, the aim of this study was to map brown trout populations inhabiting Croatian rivers of the Danube River basin and to investigate their molecular diversity and phylogeographic patterns of the established haplotypes. Materials and methods. Anal fin tissue was taken from 141 specimens of brown trout in 14 localities in the protected areas of Croatia, situated in the mountainous regions of Gorski Kotar, Žumberak, as well as Mountain Papuk in the western Slavonia. The total DNA was extracted and then the amplification of the mtDNA control region was carried out using primers Trutta-mt-F and HN20. Amplification of the 440 bp long region of the LDH-C1 gene locus was done using primers Ldhxon3F and Ldhxon4R. Amplified LDH-C* fragments were used for Restriction Fragment Length Polymorphism (RFLP) analysis using BselI restriction enzyme. Results. Analysis of the CR mtDNA revealed the presence of two phylogenetic lineages, the DA and the Atlantic (AT). Haplotypes Da1, Da2, and Da22 were recorded within the DA lineage and At1 was recorded within the AT haplogroup. Two new haplotypes were described for the first time in this study and are named Da1f and Da1g. Restriction analysis of the lactate dehydrogenase gene locus revealed a high degree of hybridization between brown trout of DA and AT haplogroups. Conclusion. The results of this study confirmed the complex molecular diversity of brown trout and the high degree of the introduction of non-native haplogroups into rivers of the Danube basin in Croatia. Conservation of native brown trout populations has become evident, as introduced allochthonous DA and AT haplogroups severely disrupt the indigenous brown trout stock.",
publisher = "Scientific Society of Szczecin",
journal = "Acta Ichthyologica et Piscatoria",
title = "Diversity of brown trout, salmo trutta (Actinopterygii: Salmoniformes: Salmonidae), in the danube river basin of croatia revealed by mitochondrial dna",
number = "3",
volume = "50",
doi = "10.3750/AIEP/02939",
pages = "291-300"
}

Kanjuh, T., Marić, A., Piria, M., Špelić, I., Maguire, I.,& Simonović, P.. (2020). Diversity of brown trout, salmo trutta (Actinopterygii: Salmoniformes: Salmonidae), in the danube river basin of croatia revealed by mitochondrial dna. in Acta Ichthyologica et Piscatoria
Scientific Society of Szczecin., 50(3), 291-300.
https://doi.org/10.3750/AIEP/02939

Kanjuh T, Marić A, Piria M, Špelić I, Maguire I, Simonović P. Diversity of brown trout, salmo trutta (Actinopterygii: Salmoniformes: Salmonidae), in the danube river basin of croatia revealed by mitochondrial dna. in Acta Ichthyologica et Piscatoria. 2020;50(3):291-300.
doi:10.3750/AIEP/02939 .

Kanjuh, Tamara, Marić, Ana, Piria, Marina, Špelić, Ivan, Maguire, Ivana, Simonović, Predrag, "Diversity of brown trout, salmo trutta (Actinopterygii: Salmoniformes: Salmonidae), in the danube river basin of croatia revealed by mitochondrial dna" in Acta Ichthyologica et Piscatoria, 50, no. 3 (2020):291-300,
https://doi.org/10.3750/AIEP/02939 . .

TY  - JOUR
AU  - Škraba Jurlina, Dubravka
AU  - Marić, Ana
AU  - Mrdak, Danilo
AU  - Kanjuh, Tamara
AU  - Špelić, Ivan
AU  - Nikolić, Vera
AU  - Piria, Marina
AU  - Simonović, Predrag
PY  - 2020
UR  - https://www.frontiersin.org/article/10.3389/fevo.2020.00188/full
UR  - https://radar.ibiss.bg.ac.rs/123456789/3850
AB  - The diversity of native trout fish Salmo spp. comprises a variety of nominal taxa in Serbia, Montenegro, and Bosnia and Herzegovina. Recent mapping of the resident trout populations detected the presence of brown trout Salmo trutta (sensu stricto) of the Atlantic (AT) mtDNA lineage introduced into populations of both tentative Danubian trout Salmo labrax and of tentative Adriatic trout Salmo farioides belonging to the Danubian (DA) and Adriatic (AD) mtDNA lineages, respectively. Introduction of the tentative Macedonian trout Salmo macedonicus of the AD lineage was also detected in a native population of the tentative S. labrax. In almost all recipient nonmigratory trout populations, a cross-breeding between native and introduced trout was detected by heterozygosity in either only the LDH-C nuclear locus or the LDH-C and specific microsatellite loci. The only exception was a population where both resident and migratory, lake-dwelling individuals of the tentative Adriatic trout spawned in a downstream section of a stream in Montenegro, as no microsatellite alleles of Atlantic brown trout that had been introduced upstream were detected. The occurrence of cross-breeding between Adriatic and brown trout was evident in the isolated, upstream section. It appears that migrating, lake-dwelling Adriatic trout in combination with their resident, stream-dwelling conspecifics suppress the introgression of genes from those situated upstream. In this regard, consideration should be given to the occurrence of the migratory brown trout in the Danube River at the broader Iron Gate Gorge area. They migrate in late summer and early fall from the Iron Gate One reservoir to the lower sections of tributaries devoid of any trout fish. However, some of these streams house very special native trout of the DA lineage in their short-extending upper sections. These native trout populations are, so far, still out of contact with the reservoir-dwelling trout. However, given the resilience of trout and their migratory life history, the outcome of this introduction could be deleterious for those native fish that are very precious in the conservation sense.
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - Alternative Life-History in Native Trout (Salmo spp.) Suppresses the Invasive Effect of Alien Trout Strains Introduced Into Streams in the Western Part of the Balkans
VL  - 8
DO  - 10.3389/fevo.2020.00188
SP  - 188
ER  - 

@article{
author = "Škraba Jurlina, Dubravka and Marić, Ana and Mrdak, Danilo and Kanjuh, Tamara and Špelić, Ivan and Nikolić, Vera and Piria, Marina and Simonović, Predrag",
year = "2020",
abstract = "The diversity of native trout fish Salmo spp. comprises a variety of nominal taxa in Serbia, Montenegro, and Bosnia and Herzegovina. Recent mapping of the resident trout populations detected the presence of brown trout Salmo trutta (sensu stricto) of the Atlantic (AT) mtDNA lineage introduced into populations of both tentative Danubian trout Salmo labrax and of tentative Adriatic trout Salmo farioides belonging to the Danubian (DA) and Adriatic (AD) mtDNA lineages, respectively. Introduction of the tentative Macedonian trout Salmo macedonicus of the AD lineage was also detected in a native population of the tentative S. labrax. In almost all recipient nonmigratory trout populations, a cross-breeding between native and introduced trout was detected by heterozygosity in either only the LDH-C nuclear locus or the LDH-C and specific microsatellite loci. The only exception was a population where both resident and migratory, lake-dwelling individuals of the tentative Adriatic trout spawned in a downstream section of a stream in Montenegro, as no microsatellite alleles of Atlantic brown trout that had been introduced upstream were detected. The occurrence of cross-breeding between Adriatic and brown trout was evident in the isolated, upstream section. It appears that migrating, lake-dwelling Adriatic trout in combination with their resident, stream-dwelling conspecifics suppress the introgression of genes from those situated upstream. In this regard, consideration should be given to the occurrence of the migratory brown trout in the Danube River at the broader Iron Gate Gorge area. They migrate in late summer and early fall from the Iron Gate One reservoir to the lower sections of tributaries devoid of any trout fish. However, some of these streams house very special native trout of the DA lineage in their short-extending upper sections. These native trout populations are, so far, still out of contact with the reservoir-dwelling trout. However, given the resilience of trout and their migratory life history, the outcome of this introduction could be deleterious for those native fish that are very precious in the conservation sense.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "Alternative Life-History in Native Trout (Salmo spp.) Suppresses the Invasive Effect of Alien Trout Strains Introduced Into Streams in the Western Part of the Balkans",
volume = "8",
doi = "10.3389/fevo.2020.00188",
pages = "188"
}

Škraba Jurlina, D., Marić, A., Mrdak, D., Kanjuh, T., Špelić, I., Nikolić, V., Piria, M.,& Simonović, P.. (2020). Alternative Life-History in Native Trout (Salmo spp.) Suppresses the Invasive Effect of Alien Trout Strains Introduced Into Streams in the Western Part of the Balkans. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 8, 188.
https://doi.org/10.3389/fevo.2020.00188

Škraba Jurlina D, Marić A, Mrdak D, Kanjuh T, Špelić I, Nikolić V, Piria M, Simonović P. Alternative Life-History in Native Trout (Salmo spp.) Suppresses the Invasive Effect of Alien Trout Strains Introduced Into Streams in the Western Part of the Balkans. in Frontiers in Ecology and Evolution. 2020;8:188.
doi:10.3389/fevo.2020.00188 .

Škraba Jurlina, Dubravka, Marić, Ana, Mrdak, Danilo, Kanjuh, Tamara, Špelić, Ivan, Nikolić, Vera, Piria, Marina, Simonović, Predrag, "Alternative Life-History in Native Trout (Salmo spp.) Suppresses the Invasive Effect of Alien Trout Strains Introduced Into Streams in the Western Part of the Balkans" in Frontiers in Ecology and Evolution, 8 (2020):188,
https://doi.org/10.3389/fevo.2020.00188 . .

TY  - JOUR
AU  - Simonović, Predrag
AU  - Marić, Ana
AU  - Škraba Jurlina, Dubravka
AU  - Kanjuh, Tamara
AU  - Nikolić, Vera
PY  - 2020
UR  - http://link.springer.com/10.2478/s11756-019-00284-1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3376
AB  - Streams’ order, their width and depth, as well as the water temperature, conductivity, dissolved oxygen contents and pH reaction were recorded for 18 streams of the Danube River basin in Serbia that home brown trout Salmo trutta. For each of them, the number of fish species was recorded, and for brown trout in them the number of cohorts, age-structure, productivity (biomass, annual production and average weight) and growth (von Betalanffy’s growth parameters L∞, t0, K and ø’) were calculated. They were examined in relation to stream’s habitat features. Stepwise multiple regression revealed strong interdependence between habitat variables. Size, i.e., width and depth of streams increased and their oxygen content dropped with increase in their order and rise of the water temperature, and water conductivity was strongly interrelated with order and depth of streams. Increase in streams’ order and their water’s temperature were accompanied with the increase of species number in them, number of brown trout cohorts raised with the water conductivity and dropped with the increase of the alkaline pH value of streams, whereas growth of brown trout was determined by width of streams and their water’s conductivity. However, when analyzed one at a time, very few of habitat and population traits, e.g., stream depth and L∞, varied clearly in streams of either various orders, or conductivity classes, respectively. The notable, but not yet significant variability of almost all other characters implicates their strong and complex acting in concert to determining number of fish species in syntopy, brown trout population, productivity and growth features.
T2  - Biologia
T1  - Determination of resident brown trout Salmo trutta features by their habitat characteristics in streams of Serbia
VL  - 75
DO  - 10.2478/s11756-019-00284-1
SP  - 103
EP  - 114
ER  - 

@article{
author = "Simonović, Predrag and Marić, Ana and Škraba Jurlina, Dubravka and Kanjuh, Tamara and Nikolić, Vera",
year = "2020",
abstract = "Streams’ order, their width and depth, as well as the water temperature, conductivity, dissolved oxygen contents and pH reaction were recorded for 18 streams of the Danube River basin in Serbia that home brown trout Salmo trutta. For each of them, the number of fish species was recorded, and for brown trout in them the number of cohorts, age-structure, productivity (biomass, annual production and average weight) and growth (von Betalanffy’s growth parameters L∞, t0, K and ø’) were calculated. They were examined in relation to stream’s habitat features. Stepwise multiple regression revealed strong interdependence between habitat variables. Size, i.e., width and depth of streams increased and their oxygen content dropped with increase in their order and rise of the water temperature, and water conductivity was strongly interrelated with order and depth of streams. Increase in streams’ order and their water’s temperature were accompanied with the increase of species number in them, number of brown trout cohorts raised with the water conductivity and dropped with the increase of the alkaline pH value of streams, whereas growth of brown trout was determined by width of streams and their water’s conductivity. However, when analyzed one at a time, very few of habitat and population traits, e.g., stream depth and L∞, varied clearly in streams of either various orders, or conductivity classes, respectively. The notable, but not yet significant variability of almost all other characters implicates their strong and complex acting in concert to determining number of fish species in syntopy, brown trout population, productivity and growth features.",
journal = "Biologia",
title = "Determination of resident brown trout Salmo trutta features by their habitat characteristics in streams of Serbia",
volume = "75",
doi = "10.2478/s11756-019-00284-1",
pages = "103-114"
}

Simonović, P., Marić, A., Škraba Jurlina, D., Kanjuh, T.,& Nikolić, V.. (2020). Determination of resident brown trout Salmo trutta features by their habitat characteristics in streams of Serbia. in Biologia, 75, 103-114.
https://doi.org/10.2478/s11756-019-00284-1

Simonović P, Marić A, Škraba Jurlina D, Kanjuh T, Nikolić V. Determination of resident brown trout Salmo trutta features by their habitat characteristics in streams of Serbia. in Biologia. 2020;75:103-114.
doi:10.2478/s11756-019-00284-1 .

Simonović, Predrag, Marić, Ana, Škraba Jurlina, Dubravka, Kanjuh, Tamara, Nikolić, Vera, "Determination of resident brown trout Salmo trutta features by their habitat characteristics in streams of Serbia" in Biologia, 75 (2020):103-114,
https://doi.org/10.2478/s11756-019-00284-1 . .

TY  - JOUR
AU  - Čanak Atlagić, Jelena
AU  - Marić, Ana
AU  - Đuknić, Jelena
AU  - Anđus, Stefan
AU  - Marinković, Nikola
AU  - Paunović, Momir
AU  - Simonović, Predrag
PY  - 2019
UR  - http://www.aiep.pl/volumes/2020/0_4/pdf/01_02591_F1.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3555
AB  - Background. A sampling of stomach and intestine content is usually performed by dissection of the gastrointestinal tract of fish. To avoid fish sacrificing, various non-lethal techniques have been developed and tested. Such sampling methods are very useful, especially for small populations and protected species. In this study, a modified syringe stomach flushing was applied on Oncorhynchus mykiss (Walbaum, 1792), and Salmo trutta Linnaeus, 1758,to test its efficiency in retrieving stomach content.Materials  and  methods.  Water was injected repeatedly by a syringe through a silicone tube directly into the stomach of live fish to flush the content out. Afterwards, fish were dissected to collect residual stomach content. The efficiency of the method was assessed as the percentage of flushed content by weight and as the percentage of the number of flushed prey items, both in respect to the whole stomach content (100%). The relation between the fish body size (length and weight) and efficiency of flushing (expressed as the weight and number of flushed prey) was tested by linear regression. The sensitivity of the method was tested with respect to 25 designated prey types. The share of each prey type was compared in the pooled sample of flushed and residual content.Results. Collected stomach content was well preserved for identification of ingested organisms. From 25 designated prey types, 17 were 100% flushed, 4 over 90%, one over 80%, 2 were 44% effectively flushed (gastropods and caddisflies in stone cases), and 1 prey type was present only in the residual sample (Gordius sp.). The efficiency of the method assessed as the mean percentage of flushed content by weight was found to be 78.78%, while 91.99% of prey items were effectively flushed.Conclusion. The applied modification of syringe stomach flushing was found to be effective for investigation of stomach content of salmonid fish since (1) collected prey items were well preserved and easily identified; (2) the percentage of flushed prey items was high (91.99%); and (3) the method is easily applicable and inexpensive
PB  - Societas Scientiarum Stetinensis (Scientific Society of Szczecin)
T2  - Acta Ichthyologica et Piscatoria
T1  - The Efficiency of Syringe Stomach Flushing in Diet Sampling of Salmonids
IS  - 4
VL  - 49
DO  - 10.3750/AIEP/02591
SP  - 319
EP  - 327
ER  - 

@article{
author = "Čanak Atlagić, Jelena and Marić, Ana and Đuknić, Jelena and Anđus, Stefan and Marinković, Nikola and Paunović, Momir and Simonović, Predrag",
year = "2019",
abstract = "Background. A sampling of stomach and intestine content is usually performed by dissection of the gastrointestinal tract of fish. To avoid fish sacrificing, various non-lethal techniques have been developed and tested. Such sampling methods are very useful, especially for small populations and protected species. In this study, a modified syringe stomach flushing was applied on Oncorhynchus mykiss (Walbaum, 1792), and Salmo trutta Linnaeus, 1758,to test its efficiency in retrieving stomach content.Materials  and  methods.  Water was injected repeatedly by a syringe through a silicone tube directly into the stomach of live fish to flush the content out. Afterwards, fish were dissected to collect residual stomach content. The efficiency of the method was assessed as the percentage of flushed content by weight and as the percentage of the number of flushed prey items, both in respect to the whole stomach content (100%). The relation between the fish body size (length and weight) and efficiency of flushing (expressed as the weight and number of flushed prey) was tested by linear regression. The sensitivity of the method was tested with respect to 25 designated prey types. The share of each prey type was compared in the pooled sample of flushed and residual content.Results. Collected stomach content was well preserved for identification of ingested organisms. From 25 designated prey types, 17 were 100% flushed, 4 over 90%, one over 80%, 2 were 44% effectively flushed (gastropods and caddisflies in stone cases), and 1 prey type was present only in the residual sample (Gordius sp.). The efficiency of the method assessed as the mean percentage of flushed content by weight was found to be 78.78%, while 91.99% of prey items were effectively flushed.Conclusion. The applied modification of syringe stomach flushing was found to be effective for investigation of stomach content of salmonid fish since (1) collected prey items were well preserved and easily identified; (2) the percentage of flushed prey items was high (91.99%); and (3) the method is easily applicable and inexpensive",
publisher = "Societas Scientiarum Stetinensis (Scientific Society of Szczecin)",
journal = "Acta Ichthyologica et Piscatoria",
title = "The Efficiency of Syringe Stomach Flushing in Diet Sampling of Salmonids",
number = "4",
volume = "49",
doi = "10.3750/AIEP/02591",
pages = "319-327"
}

Čanak Atlagić, J., Marić, A., Đuknić, J., Anđus, S., Marinković, N., Paunović, M.,& Simonović, P.. (2019). The Efficiency of Syringe Stomach Flushing in Diet Sampling of Salmonids. in Acta Ichthyologica et Piscatoria
Societas Scientiarum Stetinensis (Scientific Society of Szczecin)., 49(4), 319-327.
https://doi.org/10.3750/AIEP/02591

Čanak Atlagić J, Marić A, Đuknić J, Anđus S, Marinković N, Paunović M, Simonović P. The Efficiency of Syringe Stomach Flushing in Diet Sampling of Salmonids. in Acta Ichthyologica et Piscatoria. 2019;49(4):319-327.
doi:10.3750/AIEP/02591 .

Čanak Atlagić, Jelena, Marić, Ana, Đuknić, Jelena, Anđus, Stefan, Marinković, Nikola, Paunović, Momir, Simonović, Predrag, "The Efficiency of Syringe Stomach Flushing in Diet Sampling of Salmonids" in Acta Ichthyologica et Piscatoria, 49, no. 4 (2019):319-327,
https://doi.org/10.3750/AIEP/02591 . .

TY  - JOUR
AU  - Škraba Jurlina, Dubravka
AU  - Marić, Ana
AU  - Karanović, Jelena
AU  - Nikolić, Vera
AU  - Brkušanin, Miloš
AU  - Kanjuh, Tamara
AU  - Mrdak, Danilo
AU  - Simonović, Predrag
PY  - 2018
UR  - http://www.aiep.pl/volumes/2010/9_4/txt/txt_04.php
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3275
AB  - Background. The diversity of wild trout stocks in Montenegro is compromised by insufficiently controlled stocking. Adriatic and Mediterranean areas show a high degree of endemism of the salmonid species, with numerous native Salmo taxa described. The invasive effect of brown trout, Salmo trutta Linnaeus, 1758, of the Atlantic haplogroup on the endemic Adriatic trout, Salmo farioides Karaman, 1938, in the drainage area of the Adriatic Sea basin is lower compared to the effect inflicted by S. trutta has on Salmo labrax Pallas, 1814 of the Danubian haplogroup. The presently reported study was intended to describe the genetic structure of the population of Adriatic trout, Salmo farioides, from the Mrtvica River, a right tributary of the Morača River, Montenegro, in order to get an insight into the spread of non-native strains and their introgression into the native Adriatic trout gene pool. Materials and methods. Trout specimens sampled from the lower section of the Mrtvica River from 2004 to 2007, and from its upper section in the spring of 2014, were analysed for their mtDNA haplotypes. Nuclear DNA markers (microsatellites, LDH-C1* gene) were also included in genetic analyses since mtDNA is inherited only maternally. After statistical analyses, relations between individuals from the upper and lower Mrtvica River were reconstructed. Results. Using both LDH-C1* and seven microsatellite loci with several alleles exclusive to S. trutta, an introgression was detected only in the upper reaches of the Mrtvica River, where only stream-dwelling trout form occurs. In the lower reaches no introgression was detected at all, as revealed by the absence of both the Atlantic mitochondrial haplotype and microsatellite alleles exclusive to S. trutta. Conclusion. The allelic frequency at seven microsatellite loci of Salmo spp. from the two river sections revealed very different population structure, as a consequence of a low rate of gene flow between them. In addition to the physical barrier that prevents Salmo sp. from the lower Mrtvica River to migrate to the upper river section, it seems that the opportunity to mate with the large pool of conspecifics, including lake-dwelling S. farioides (i.e., Salmo cf. dentex), prevents Salmo trutta from surviving at the lower stretch and spread to the rest of the Morača River and Lake Skadar systems.
T2  - Acta Ichthyologica et Piscatoria
T1  - Effect of the introgression of Atlantic brown trout, Salmo trutta, into Adriatic trout, Salmo farioides in a stream at the drainage area of the Adriatic Sea basin of Montenegro
IS  - 4
VL  - 48
DO  - 10.3750/AIEP/02491
SP  - 363
EP  - 372
ER  - 

@article{
author = "Škraba Jurlina, Dubravka and Marić, Ana and Karanović, Jelena and Nikolić, Vera and Brkušanin, Miloš and Kanjuh, Tamara and Mrdak, Danilo and Simonović, Predrag",
year = "2018",
abstract = "Background. The diversity of wild trout stocks in Montenegro is compromised by insufficiently controlled stocking. Adriatic and Mediterranean areas show a high degree of endemism of the salmonid species, with numerous native Salmo taxa described. The invasive effect of brown trout, Salmo trutta Linnaeus, 1758, of the Atlantic haplogroup on the endemic Adriatic trout, Salmo farioides Karaman, 1938, in the drainage area of the Adriatic Sea basin is lower compared to the effect inflicted by S. trutta has on Salmo labrax Pallas, 1814 of the Danubian haplogroup. The presently reported study was intended to describe the genetic structure of the population of Adriatic trout, Salmo farioides, from the Mrtvica River, a right tributary of the Morača River, Montenegro, in order to get an insight into the spread of non-native strains and their introgression into the native Adriatic trout gene pool. Materials and methods. Trout specimens sampled from the lower section of the Mrtvica River from 2004 to 2007, and from its upper section in the spring of 2014, were analysed for their mtDNA haplotypes. Nuclear DNA markers (microsatellites, LDH-C1* gene) were also included in genetic analyses since mtDNA is inherited only maternally. After statistical analyses, relations between individuals from the upper and lower Mrtvica River were reconstructed. Results. Using both LDH-C1* and seven microsatellite loci with several alleles exclusive to S. trutta, an introgression was detected only in the upper reaches of the Mrtvica River, where only stream-dwelling trout form occurs. In the lower reaches no introgression was detected at all, as revealed by the absence of both the Atlantic mitochondrial haplotype and microsatellite alleles exclusive to S. trutta. Conclusion. The allelic frequency at seven microsatellite loci of Salmo spp. from the two river sections revealed very different population structure, as a consequence of a low rate of gene flow between them. In addition to the physical barrier that prevents Salmo sp. from the lower Mrtvica River to migrate to the upper river section, it seems that the opportunity to mate with the large pool of conspecifics, including lake-dwelling S. farioides (i.e., Salmo cf. dentex), prevents Salmo trutta from surviving at the lower stretch and spread to the rest of the Morača River and Lake Skadar systems.",
journal = "Acta Ichthyologica et Piscatoria",
title = "Effect of the introgression of Atlantic brown trout, Salmo trutta, into Adriatic trout, Salmo farioides in a stream at the drainage area of the Adriatic Sea basin of Montenegro",
number = "4",
volume = "48",
doi = "10.3750/AIEP/02491",
pages = "363-372"
}

Škraba Jurlina, D., Marić, A., Karanović, J., Nikolić, V., Brkušanin, M., Kanjuh, T., Mrdak, D.,& Simonović, P.. (2018). Effect of the introgression of Atlantic brown trout, Salmo trutta, into Adriatic trout, Salmo farioides in a stream at the drainage area of the Adriatic Sea basin of Montenegro. in Acta Ichthyologica et Piscatoria, 48(4), 363-372.
https://doi.org/10.3750/AIEP/02491

Škraba Jurlina D, Marić A, Karanović J, Nikolić V, Brkušanin M, Kanjuh T, Mrdak D, Simonović P. Effect of the introgression of Atlantic brown trout, Salmo trutta, into Adriatic trout, Salmo farioides in a stream at the drainage area of the Adriatic Sea basin of Montenegro. in Acta Ichthyologica et Piscatoria. 2018;48(4):363-372.
doi:10.3750/AIEP/02491 .

Škraba Jurlina, Dubravka, Marić, Ana, Karanović, Jelena, Nikolić, Vera, Brkušanin, Miloš, Kanjuh, Tamara, Mrdak, Danilo, Simonović, Predrag, "Effect of the introgression of Atlantic brown trout, Salmo trutta, into Adriatic trout, Salmo farioides in a stream at the drainage area of the Adriatic Sea basin of Montenegro" in Acta Ichthyologica et Piscatoria, 48, no. 4 (2018):363-372,
https://doi.org/10.3750/AIEP/02491 . .