TY  - CONF
AU  - Farnleitner, Andreas
AU  - Schachner, Iris
AU  - Jakwerth, Stefan
AU  - Kittinger, Clemens
AU  - Zarfel, Gernot
AU  - Kavka, Gerhard
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Toth, Erika
AU  - Blaschke, Alfred
AU  - Derx, Julia
AU  - Linke, Rita
AU  - Demeter, Katalin
AU  - Savio, Domenico
AU  - Resicher, Georg
AU  - Kir, Alexander
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6596
AB  - Sustainable and target-oriented microbiological water quality management of rivers needs
information on whole river systems, especially if catchments are large and international. The
microbiological water quality of rivers is of uttermost relevance for human health as river water is
used for several purposes (recreation, drinking water production, irrigation). Besides information on
faecal pollution levels, the origin of faecal pollution and the assessment of associated infection- and
health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This
contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and
discusses future challenges and perspectives (pollution-scenario modelling, infection-risk
assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS 1 to 4. Beside
standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including
microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput ampliconsequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were
isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big
picture” of the microbiological pollution status of the Danube River. Harmonised trans-border
microbiological water quality maps for the whole navigable Danube were established. MST marker
analysis elucidated that the main faecal pollution source along the whole river is human wastewater.
Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the
potential health impacts of AMR associated with faecal pollution
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6596
ER  - 

@conference{
author = "Farnleitner, Andreas and Schachner, Iris and Jakwerth, Stefan and Kittinger, Clemens and Zarfel, Gernot and Kavka, Gerhard and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Toth, Erika and Blaschke, Alfred and Derx, Julia and Linke, Rita and Demeter, Katalin and Savio, Domenico and Resicher, Georg and Kir, Alexander",
year = "2023",
abstract = "Sustainable and target-oriented microbiological water quality management of rivers needs
information on whole river systems, especially if catchments are large and international. The
microbiological water quality of rivers is of uttermost relevance for human health as river water is
used for several purposes (recreation, drinking water production, irrigation). Besides information on
faecal pollution levels, the origin of faecal pollution and the assessment of associated infection- and
health risks, e g. from antimicrobial resistant bacteria (ARB) are of increasing importance. This
contribution summarizes the concepts and main results from the Joint-Danube-Surveys (JDS) and
discusses future challenges and perspectives (pollution-scenario modelling, infection-risk
assessment) for the Danube River concerning its faecal and AMR pollution status.
Between 2001 and 2019, the whole Danube was sampled four times during the JDS 1 to 4. Beside
standard faecal indicator analysis, cutting-edge molecular detection concepts were applied, including
microbial source tracking (MST) markers by quantitative PCR (qPCR) and high-throughput ampliconsequencing of bacterial communities. For AMR-profiling, clinically relevant bacterial species were
isolated and tested for resistances and resistance genes were determined via qPCR.
With this, we could impressively demonstrate that the JDS create the required multi-national “big
picture” of the microbiological pollution status of the Danube River. Harmonised trans-border
microbiological water quality maps for the whole navigable Danube were established. MST marker
analysis elucidated that the main faecal pollution source along the whole river is human wastewater.
Combined analysis of faecal indicators, MST and AMR-profiles provided a solid basis for assessing the
potential health impacts of AMR associated with faecal pollution",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance",
pages = "9",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6596"
}

Farnleitner, A., Schachner, I., Jakwerth, S., Kittinger, C., Zarfel, G., Kavka, G., Kolarević, S., Kračun-Kolarević, M., Toth, E., Blaschke, A., Derx, J., Linke, R., Demeter, K., Savio, D., Resicher, G.,& Kir, A.. (2023). Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 9.
https://hdl.handle.net/21.15107/rcub_ibiss_6596

Farnleitner A, Schachner I, Jakwerth S, Kittinger C, Zarfel G, Kavka G, Kolarević S, Kračun-Kolarević M, Toth E, Blaschke A, Derx J, Linke R, Demeter K, Savio D, Resicher G, Kir A. Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:9.
https://hdl.handle.net/21.15107/rcub_ibiss_6596 .

Farnleitner, Andreas, Schachner, Iris, Jakwerth, Stefan, Kittinger, Clemens, Zarfel, Gernot, Kavka, Gerhard, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Toth, Erika, Blaschke, Alfred, Derx, Julia, Linke, Rita, Demeter, Katalin, Savio, Domenico, Resicher, Georg, Kir, Alexander, "Joint Danube Survey 1 to 4: Concepts, Lessons Learned and Future Visions on Faecal Pollution and Antimicrobial Resistance" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):9,
https://hdl.handle.net/21.15107/rcub_ibiss_6596 .