TY  - JOUR
AU  - Stojanović, Jelena
AU  - Savić-Zdravković, Dimitrija
AU  - Jovanović, Boris
AU  - Vitorović, Jelena
AU  - Bašić, Jelena
AU  - Stojanović, Ivana
AU  - Žabar Popović, Andrea
AU  - Duran, Hatice
AU  - Kračun-Kolarević, Margareta
AU  - Milošević, Đurađ
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6430
AB  - In the last few decades, industrial pollution has gained extensive attention in terms of its effect on the aquatic environment. This imposes the need to develop sensitive biomarkers for early detection of pollutant toxicity in ecotoxicological assessment. The advantages of histopathological biomarkers are many, including quick reaction to the presence of contaminants, and the small number of individuals needed for efficient analysis. The present study analyzed the negative effect of lignite coal fly ash (LCFA) and microplastic particles (MPs) on Chironomus riparius, a suggested model organism by the Organization for Economic Cooperation and Development (OECD). This study aimed to perform histological analyses of larval tissues and target potential changes in treated groups that could serve as promising histopathological biomarkers of the contaminant's negative effects. Following that, other known sensitive sub-organismal biomarkers were analyzed and paired with the histopathological ones. Histological analysis of larvae showed a significantly decreased length of microvilli in midgut regions II and III in both treatments. Treatments with MPs affected oxidative stress parameters: thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP), superoxide dismutase (SOD), and hemoglobin levels, while LCFA significantly affected all tested sub-organismal biomarkers (DNA damage, levels of AOPP, SOD, and hemoglobin), except catalase (CAT) and TBARS. When observing histological slides, a significant shortage of brush border length in the posterior parts of the midgut was detected in all treatments. In the case of LCFA, the appearance of intensive vacuolization of digestive cells with inclusions resembling apoptotic bodies, in mentioned regions was also detected. This study demonstrated high sensitivity of brush border length to the MPs and LCFA exposure, complementary to other tested sub-organismal biomarkers. Revealing the great potential of this histopathological biomarker in ecotoxicological studies contributes to the international standard ecotoxicology assessment of emerging pollutants.
PB  - Elsevier
T2  - Science of The Total Environment
T1  - Histopathology of chironomids exposed to fly ash and microplastics as a new biomarker of ecotoxicological assessment
IS  - 166042
VL  - 903
DO  - 10.1016/j.scitotenv.2023.166042
ER  - 

@article{
author = "Stojanović, Jelena and Savić-Zdravković, Dimitrija and Jovanović, Boris and Vitorović, Jelena and Bašić, Jelena and Stojanović, Ivana and Žabar Popović, Andrea and Duran, Hatice and Kračun-Kolarević, Margareta and Milošević, Đurađ",
year = "2023",
abstract = "In the last few decades, industrial pollution has gained extensive attention in terms of its effect on the aquatic environment. This imposes the need to develop sensitive biomarkers for early detection of pollutant toxicity in ecotoxicological assessment. The advantages of histopathological biomarkers are many, including quick reaction to the presence of contaminants, and the small number of individuals needed for efficient analysis. The present study analyzed the negative effect of lignite coal fly ash (LCFA) and microplastic particles (MPs) on Chironomus riparius, a suggested model organism by the Organization for Economic Cooperation and Development (OECD). This study aimed to perform histological analyses of larval tissues and target potential changes in treated groups that could serve as promising histopathological biomarkers of the contaminant's negative effects. Following that, other known sensitive sub-organismal biomarkers were analyzed and paired with the histopathological ones. Histological analysis of larvae showed a significantly decreased length of microvilli in midgut regions II and III in both treatments. Treatments with MPs affected oxidative stress parameters: thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP), superoxide dismutase (SOD), and hemoglobin levels, while LCFA significantly affected all tested sub-organismal biomarkers (DNA damage, levels of AOPP, SOD, and hemoglobin), except catalase (CAT) and TBARS. When observing histological slides, a significant shortage of brush border length in the posterior parts of the midgut was detected in all treatments. In the case of LCFA, the appearance of intensive vacuolization of digestive cells with inclusions resembling apoptotic bodies, in mentioned regions was also detected. This study demonstrated high sensitivity of brush border length to the MPs and LCFA exposure, complementary to other tested sub-organismal biomarkers. Revealing the great potential of this histopathological biomarker in ecotoxicological studies contributes to the international standard ecotoxicology assessment of emerging pollutants.",
publisher = "Elsevier",
journal = "Science of The Total Environment",
title = "Histopathology of chironomids exposed to fly ash and microplastics as a new biomarker of ecotoxicological assessment",
number = "166042",
volume = "903",
doi = "10.1016/j.scitotenv.2023.166042"
}

Stojanović, J., Savić-Zdravković, D., Jovanović, B., Vitorović, J., Bašić, J., Stojanović, I., Žabar Popović, A., Duran, H., Kračun-Kolarević, M.,& Milošević, Đ.. (2023). Histopathology of chironomids exposed to fly ash and microplastics as a new biomarker of ecotoxicological assessment. in Science of The Total Environment
Elsevier., 903(166042).
https://doi.org/10.1016/j.scitotenv.2023.166042

Stojanović J, Savić-Zdravković D, Jovanović B, Vitorović J, Bašić J, Stojanović I, Žabar Popović A, Duran H, Kračun-Kolarević M, Milošević Đ. Histopathology of chironomids exposed to fly ash and microplastics as a new biomarker of ecotoxicological assessment. in Science of The Total Environment. 2023;903(166042).
doi:10.1016/j.scitotenv.2023.166042 .

Stojanović, Jelena, Savić-Zdravković, Dimitrija, Jovanović, Boris, Vitorović, Jelena, Bašić, Jelena, Stojanović, Ivana, Žabar Popović, Andrea, Duran, Hatice, Kračun-Kolarević, Margareta, Milošević, Đurađ, "Histopathology of chironomids exposed to fly ash and microplastics as a new biomarker of ecotoxicological assessment" in Science of The Total Environment, 903, no. 166042 (2023),
https://doi.org/10.1016/j.scitotenv.2023.166042 . .

TY  - JOUR
AU  - Stevanović, Ivana
AU  - Mančić, Bojana
AU  - Ilić, Tihomir
AU  - Milosavljević, Petar
AU  - Lavrnja, Irena
AU  - Stojanović, Ivana
AU  - Ninković, Milica
PY  - 2019
UR  - https://www.termedia.pl/doi/10.5114/fn.2019.86294
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3480
AB  - Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freund's adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rat's spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions.
T2  - Folia Neuropathologica
T1  - Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis.
IS  - 2
VL  - 57
DO  - 10.5114/fn.2019.86294
SP  - 129
EP  - 145
ER  - 

@article{
author = "Stevanović, Ivana and Mančić, Bojana and Ilić, Tihomir and Milosavljević, Petar and Lavrnja, Irena and Stojanović, Ivana and Ninković, Milica",
year = "2019",
abstract = "Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freund's adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rat's spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions.",
journal = "Folia Neuropathologica",
title = "Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis.",
number = "2",
volume = "57",
doi = "10.5114/fn.2019.86294",
pages = "129-145"
}

Stevanović, I., Mančić, B., Ilić, T., Milosavljević, P., Lavrnja, I., Stojanović, I.,& Ninković, M.. (2019). Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis.. in Folia Neuropathologica, 57(2), 129-145.
https://doi.org/10.5114/fn.2019.86294

Stevanović I, Mančić B, Ilić T, Milosavljević P, Lavrnja I, Stojanović I, Ninković M. Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis.. in Folia Neuropathologica. 2019;57(2):129-145.
doi:10.5114/fn.2019.86294 .

Stevanović, Ivana, Mančić, Bojana, Ilić, Tihomir, Milosavljević, Petar, Lavrnja, Irena, Stojanović, Ivana, Ninković, Milica, "Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis." in Folia Neuropathologica, 57, no. 2 (2019):129-145,
https://doi.org/10.5114/fn.2019.86294 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Vuković-Dejanović, Vesna
AU  - Ninković, Milica
AU  - Lavrnja, Irena
AU  - Stojanović, Ivana
AU  - Pavlović, Miloš
AU  - Begović, Vesna
AU  - Mirković, Duško
AU  - Stevanović, Ivana
PY  - 2018
UR  - https://actavet.vfu.cz/87/2/0145/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3097
AB  - This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.
T2  - Acta Veterinaria Brno
T1  - Effects of agmatine on chlorpromazine-induced neuronal injury in rat
IS  - 2
VL  - 87
DO  - 10.2754/avb201887020145
DO  - 0001-7213
SP  - 145
EP  - 153
ER  - 

@article{
author = "Dejanović, Bratislav and Vuković-Dejanović, Vesna and Ninković, Milica and Lavrnja, Irena and Stojanović, Ivana and Pavlović, Miloš and Begović, Vesna and Mirković, Duško and Stevanović, Ivana",
year = "2018",
abstract = "This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.",
journal = "Acta Veterinaria Brno",
title = "Effects of agmatine on chlorpromazine-induced neuronal injury in rat",
number = "2",
volume = "87",
doi = "10.2754/avb201887020145, 0001-7213",
pages = "145-153"
}

Dejanović, B., Vuković-Dejanović, V., Ninković, M., Lavrnja, I., Stojanović, I., Pavlović, M., Begović, V., Mirković, D.,& Stevanović, I.. (2018). Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno, 87(2), 145-153.
https://doi.org/10.2754/avb201887020145

Dejanović B, Vuković-Dejanović V, Ninković M, Lavrnja I, Stojanović I, Pavlović M, Begović V, Mirković D, Stevanović I. Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno. 2018;87(2):145-153.
doi:10.2754/avb201887020145 .

Dejanović, Bratislav, Vuković-Dejanović, Vesna, Ninković, Milica, Lavrnja, Irena, Stojanović, Ivana, Pavlović, Miloš, Begović, Vesna, Mirković, Duško, Stevanović, Ivana, "Effects of agmatine on chlorpromazine-induced neuronal injury in rat" in Acta Veterinaria Brno, 87, no. 2 (2018):145-153,
https://doi.org/10.2754/avb201887020145 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Lavrnja, Irena
AU  - Ninković, Milica
AU  - Stojanović, Ivana
AU  - Đurić, Ana
AU  - Dilber, Sanda
AU  - Stevanović, Ivana
PY  - 2017
UR  - https://www.jstage.jst.go.jp/article/expanim/66/1/66_16-0010/_article
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2562
AB  - Chlorpromazine (CPZ) is a member of a widely used class of antipsychotic agents. The metabolic pathways of CPZ toxicity were examined by monitoring oxidative/nitrosative stress markers. The aim of the study was to investigate the hypothesis that agmatine (AGM) prevents oxidative stress in the liver of Wistar rats 48 h after administration of CPZ. All tested compounds were administered intraperitoneally (i.p.) in one single dose. The animals were divided into control (C, 0.9% saline solution), CPZ (CPZ, 38.7 mg/kg b.w.), CPZ+AGM (AGM, 75 mg/kg b.w. immediately after CPZ, 38.7 mg/kg b.w. i.p.), and AGM (AGM, 75 mg/kg b.w.) groups. Rats were sacrificed by decapitation 48 h after treatment. The CPZ and CPZ+AGM treatments significantly increased thiobarbituric acid reactive substances (TBARS), the nitrite and nitrate (NO2+NO3) concentration, and superoxide anion (O2 •-) production in rat liver homogenates compared with C values. CPZ injection decreased the capacity of the antioxidant defense system: superoxide dismutase (SOD) activity, catalase (CAT) activity, total glutathione (GSH) content, glutathione peroxidase (GPx) activity, and glutathione reductase (GR) activity compared with the values of the C group. However, treatment with AGM increased antioxidant capacity in the rat liver; it increased the CAT activity, GSH concentration, GPx activity, and GR activity compared with the values of the CPZ rats. Immunohistochemical staining of ED1 in rats showed an increase in the number of positive cells 48 h after acute CPZ administration compared with the C group. Our results showed that AGM has no protective effects on parameters of oxidative and/or nitrosative stress in the liver but that it absolutely protective effects on the antioxidant defense system and restores the antioxidant capacity in liver tissue after administration of CPZ.
T2  - Experimental Animals
T1  - Effects of agmatine on chlorpromazine toxicity in the liver of Wistar rats: the possible role of oxidant/antioxidant imbalance
IS  - 1
VL  - 66
DO  - 10.1538/expanim.16-0010
SP  - 17
EP  - 27
ER  - 

@article{
author = "Dejanović, Bratislav and Lavrnja, Irena and Ninković, Milica and Stojanović, Ivana and Đurić, Ana and Dilber, Sanda and Stevanović, Ivana",
year = "2017",
abstract = "Chlorpromazine (CPZ) is a member of a widely used class of antipsychotic agents. The metabolic pathways of CPZ toxicity were examined by monitoring oxidative/nitrosative stress markers. The aim of the study was to investigate the hypothesis that agmatine (AGM) prevents oxidative stress in the liver of Wistar rats 48 h after administration of CPZ. All tested compounds were administered intraperitoneally (i.p.) in one single dose. The animals were divided into control (C, 0.9% saline solution), CPZ (CPZ, 38.7 mg/kg b.w.), CPZ+AGM (AGM, 75 mg/kg b.w. immediately after CPZ, 38.7 mg/kg b.w. i.p.), and AGM (AGM, 75 mg/kg b.w.) groups. Rats were sacrificed by decapitation 48 h after treatment. The CPZ and CPZ+AGM treatments significantly increased thiobarbituric acid reactive substances (TBARS), the nitrite and nitrate (NO2+NO3) concentration, and superoxide anion (O2 •-) production in rat liver homogenates compared with C values. CPZ injection decreased the capacity of the antioxidant defense system: superoxide dismutase (SOD) activity, catalase (CAT) activity, total glutathione (GSH) content, glutathione peroxidase (GPx) activity, and glutathione reductase (GR) activity compared with the values of the C group. However, treatment with AGM increased antioxidant capacity in the rat liver; it increased the CAT activity, GSH concentration, GPx activity, and GR activity compared with the values of the CPZ rats. Immunohistochemical staining of ED1 in rats showed an increase in the number of positive cells 48 h after acute CPZ administration compared with the C group. Our results showed that AGM has no protective effects on parameters of oxidative and/or nitrosative stress in the liver but that it absolutely protective effects on the antioxidant defense system and restores the antioxidant capacity in liver tissue after administration of CPZ.",
journal = "Experimental Animals",
title = "Effects of agmatine on chlorpromazine toxicity in the liver of Wistar rats: the possible role of oxidant/antioxidant imbalance",
number = "1",
volume = "66",
doi = "10.1538/expanim.16-0010",
pages = "17-27"
}

Dejanović, B., Lavrnja, I., Ninković, M., Stojanović, I., Đurić, A., Dilber, S.,& Stevanović, I.. (2017). Effects of agmatine on chlorpromazine toxicity in the liver of Wistar rats: the possible role of oxidant/antioxidant imbalance. in Experimental Animals, 66(1), 17-27.
https://doi.org/10.1538/expanim.16-0010

Dejanović B, Lavrnja I, Ninković M, Stojanović I, Đurić A, Dilber S, Stevanović I. Effects of agmatine on chlorpromazine toxicity in the liver of Wistar rats: the possible role of oxidant/antioxidant imbalance. in Experimental Animals. 2017;66(1):17-27.
doi:10.1538/expanim.16-0010 .

Dejanović, Bratislav, Lavrnja, Irena, Ninković, Milica, Stojanović, Ivana, Đurić, Ana, Dilber, Sanda, Stevanović, Ivana, "Effects of agmatine on chlorpromazine toxicity in the liver of Wistar rats: the possible role of oxidant/antioxidant imbalance" in Experimental Animals, 66, no. 1 (2017):17-27,
https://doi.org/10.1538/expanim.16-0010 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Stevanović, Ivana
AU  - Ninković, Milica
AU  - Stojanović, Ivana
AU  - Lavrnja, Irena
AU  - Radičević, Tatjana
PY  - 2016
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84964311353&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2531
AB  - Summary The metabolic pathways of chlorpromazine (CPZ) toxicity were tracked by assessing oxidative/nitrosative stress markers. The main objective of the study was to test the hypothesis that agmatine (AGM) prevents oxidative/nitrosative stress in the liver of Wistar rats 15 days after administration of CPZ. All tested substances were administered intraperitoneally (i.p.) for 15 consecutive days. The rats were divided into four groups: the control group (C, 0.9 % saline solution), the CPZ group (CPZ, 38.7 mg/kg b.w.), the CPZ+AGM group (AGM, 75 mg/kg b.w. immediately after CPZ, 38.7 mg/kg b.w. i.p.) and the AGM group (AGM, 75 mg/kg b.w.). Rats were decapitated 15 days after the appropriate treatment. In the CPZ group, CPZ concentration was significantly increased compared to C values (p<0.01), while AGM treatment induced the significant decrease in CPZ concentration in the CPZ+AGM group (p<0.05) and the AGM group (p<0.01). CPZ application to healthy rats did not lead to any changes of lipid peroxidation in the liver compared to the C group, but AGM treatment decreased that parameter compared to the CPZ group (p<0.05). In CPZ liver homogenates, nitrite and nitrate concentrations were increased compared to controls (p<0.001), and AGM treatment diminished that parameter in the CPZ group (p<0.05), as well as in the AGM group (p<0.001). In CPZ animals, glutathione level and catalase activity were decreased in comparison with C values (p<0.01 respectively), but AGM treatment increased the activity of catalase in comparison with CPZ animals (p<0.05 respectively). Western blot analysis supported biochemical findings in all groups. Our results showed that treatment with AGM significantly supressed the oxidative/nitrosative stress parameters and restored antioxidant defense in rat liver.
T2  - Acta Facultatis Medicae Naissensis
T1  - Protective effects of agmatine against chlorpromazine-induced toxicity in the liver of wistar rats
IS  - 1
VL  - 33
DO  - 10.1515/afmnai-2016-0002
SP  - 13
EP  - 22
ER  - 

@article{
author = "Dejanović, Bratislav and Stevanović, Ivana and Ninković, Milica and Stojanović, Ivana and Lavrnja, Irena and Radičević, Tatjana",
year = "2016",
abstract = "Summary The metabolic pathways of chlorpromazine (CPZ) toxicity were tracked by assessing oxidative/nitrosative stress markers. The main objective of the study was to test the hypothesis that agmatine (AGM) prevents oxidative/nitrosative stress in the liver of Wistar rats 15 days after administration of CPZ. All tested substances were administered intraperitoneally (i.p.) for 15 consecutive days. The rats were divided into four groups: the control group (C, 0.9 % saline solution), the CPZ group (CPZ, 38.7 mg/kg b.w.), the CPZ+AGM group (AGM, 75 mg/kg b.w. immediately after CPZ, 38.7 mg/kg b.w. i.p.) and the AGM group (AGM, 75 mg/kg b.w.). Rats were decapitated 15 days after the appropriate treatment. In the CPZ group, CPZ concentration was significantly increased compared to C values (p<0.01), while AGM treatment induced the significant decrease in CPZ concentration in the CPZ+AGM group (p<0.05) and the AGM group (p<0.01). CPZ application to healthy rats did not lead to any changes of lipid peroxidation in the liver compared to the C group, but AGM treatment decreased that parameter compared to the CPZ group (p<0.05). In CPZ liver homogenates, nitrite and nitrate concentrations were increased compared to controls (p<0.001), and AGM treatment diminished that parameter in the CPZ group (p<0.05), as well as in the AGM group (p<0.001). In CPZ animals, glutathione level and catalase activity were decreased in comparison with C values (p<0.01 respectively), but AGM treatment increased the activity of catalase in comparison with CPZ animals (p<0.05 respectively). Western blot analysis supported biochemical findings in all groups. Our results showed that treatment with AGM significantly supressed the oxidative/nitrosative stress parameters and restored antioxidant defense in rat liver.",
journal = "Acta Facultatis Medicae Naissensis",
title = "Protective effects of agmatine against chlorpromazine-induced toxicity in the liver of wistar rats",
number = "1",
volume = "33",
doi = "10.1515/afmnai-2016-0002",
pages = "13-22"
}

Dejanović, B., Stevanović, I., Ninković, M., Stojanović, I., Lavrnja, I.,& Radičević, T.. (2016). Protective effects of agmatine against chlorpromazine-induced toxicity in the liver of wistar rats. in Acta Facultatis Medicae Naissensis, 33(1), 13-22.
https://doi.org/10.1515/afmnai-2016-0002

Dejanović B, Stevanović I, Ninković M, Stojanović I, Lavrnja I, Radičević T. Protective effects of agmatine against chlorpromazine-induced toxicity in the liver of wistar rats. in Acta Facultatis Medicae Naissensis. 2016;33(1):13-22.
doi:10.1515/afmnai-2016-0002 .

Dejanović, Bratislav, Stevanović, Ivana, Ninković, Milica, Stojanović, Ivana, Lavrnja, Irena, Radičević, Tatjana, "Protective effects of agmatine against chlorpromazine-induced toxicity in the liver of wistar rats" in Acta Facultatis Medicae Naissensis, 33, no. 1 (2016):13-22,
https://doi.org/10.1515/afmnai-2016-0002 . .

TY  - JOUR
AU  - Stevanović, Ivana
AU  - Jovanović, Marina
AU  - Jelenković, Ankica V.
AU  - Bokonjić, D.
AU  - Čolić, M.
AU  - Stojanović, Ivana
AU  - Ninković, Milica
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/475
AB  - The present experiment was carried out to determine the effectiveness of nitric oxide synthase (NOS) inhibitor (L-NAME) in elevating the toxicity of AlCl3 on nitrite concentration and acetylcholine esterase activity of Wistar rats. Animals were killed 10 min and 3 days after the treatment and the forebrain cortex and striatum were removed. The results show that AlCl3 exposure promotes oxidative stress in different neural areas. The biochemical changes observed in neuronal tissues show that aluminium acts as a pro-oxidant, while NOS inhibitor exerts as antioxidant action in AlCl3-treated animals. In the present study, active avoidance learning was significantly impaired after AlCl3 injection, while pretreatment with L-NAME prevented the behavioural deficits caused between the 8th and 12th day after intrahippocampal application of neurotoxin. Our data suggest that aluminium may cause learning and memory deficits, while the treatment with L-NAME may decrease the oxidative stress and prevent learning and memory deficits caused by AlCl3.
AB  - U eksperimentu je ispitivana efikasnost inhibitora azot oksid sintaze (NOS)- L-NAME na toksičnost AlCl3 i određivana koncentracija nitrita i aktivnost acetilholin esteraze kod Wistar pacova. Životinje su dekapitovane 10 minuta ili 3 dana nakon tretmana i izolovani su kora prednjeg mozga i strijatum. Rezultati ukazuju da AlCl3 pokreće oksidativni stres u različitim regionima mozga. Biohemijske promene opisane u neuronskom tkivu ukazuju da aluminijum deluje kao prooksidans, dok inhibitor NOS ima antioksidativno dejstvo kod životinja tretiranih AlCl3. Reakcija aktivnog izbegavanja je bila znatno poremećena nakon aplikacije AlCl3, dok se davanjem L-NAME sprečavaju poremećaji ponašanja uzrokovani između 8. i 12. dana posle intrahipokampusne primene neurotoksina. Naši rezultati ukazuju da aluminijum može dovesti do smetnji u procesima učenja i pamćenja, dok tretman sa L-NAME smanjuje oksidativni stres i sprečava promene u učenju i pamćenju uzrokovane AlCl3.
T2  - Acta veterinaria
T1  - Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova
T1  - Effect of L-NAME on AlCl3-induced toxicity in rat brain
IS  - 2-3
VL  - 59
DO  - 10.2298/AVB0903133S
SP  - 133
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_475
ER  - 

@article{
author = "Stevanović, Ivana and Jovanović, Marina and Jelenković, Ankica V. and Bokonjić, D. and Čolić, M. and Stojanović, Ivana and Ninković, Milica",
year = "2009, 2009",
abstract = "The present experiment was carried out to determine the effectiveness of nitric oxide synthase (NOS) inhibitor (L-NAME) in elevating the toxicity of AlCl3 on nitrite concentration and acetylcholine esterase activity of Wistar rats. Animals were killed 10 min and 3 days after the treatment and the forebrain cortex and striatum were removed. The results show that AlCl3 exposure promotes oxidative stress in different neural areas. The biochemical changes observed in neuronal tissues show that aluminium acts as a pro-oxidant, while NOS inhibitor exerts as antioxidant action in AlCl3-treated animals. In the present study, active avoidance learning was significantly impaired after AlCl3 injection, while pretreatment with L-NAME prevented the behavioural deficits caused between the 8th and 12th day after intrahippocampal application of neurotoxin. Our data suggest that aluminium may cause learning and memory deficits, while the treatment with L-NAME may decrease the oxidative stress and prevent learning and memory deficits caused by AlCl3., U eksperimentu je ispitivana efikasnost inhibitora azot oksid sintaze (NOS)- L-NAME na toksičnost AlCl3 i određivana koncentracija nitrita i aktivnost acetilholin esteraze kod Wistar pacova. Životinje su dekapitovane 10 minuta ili 3 dana nakon tretmana i izolovani su kora prednjeg mozga i strijatum. Rezultati ukazuju da AlCl3 pokreće oksidativni stres u različitim regionima mozga. Biohemijske promene opisane u neuronskom tkivu ukazuju da aluminijum deluje kao prooksidans, dok inhibitor NOS ima antioksidativno dejstvo kod životinja tretiranih AlCl3. Reakcija aktivnog izbegavanja je bila znatno poremećena nakon aplikacije AlCl3, dok se davanjem L-NAME sprečavaju poremećaji ponašanja uzrokovani između 8. i 12. dana posle intrahipokampusne primene neurotoksina. Naši rezultati ukazuju da aluminijum može dovesti do smetnji u procesima učenja i pamćenja, dok tretman sa L-NAME smanjuje oksidativni stres i sprečava promene u učenju i pamćenju uzrokovane AlCl3.",
journal = "Acta veterinaria",
title = "Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova, Effect of L-NAME on AlCl3-induced toxicity in rat brain",
number = "2-3",
volume = "59",
doi = "10.2298/AVB0903133S",
pages = "133-146",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_475"
}

Stevanović, I., Jovanović, M., Jelenković, A. V., Bokonjić, D., Čolić, M., Stojanović, I.,& Ninković, M.. (2009). Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova. in Acta veterinaria, 59(2-3), 133-146.
https://doi.org/10.2298/AVB0903133S
https://hdl.handle.net/21.15107/rcub_ibiss_475

Stevanović I, Jovanović M, Jelenković AV, Bokonjić D, Čolić M, Stojanović I, Ninković M. Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova. in Acta veterinaria. 2009;59(2-3):133-146.
doi:10.2298/AVB0903133S
https://hdl.handle.net/21.15107/rcub_ibiss_475 .

Stevanović, Ivana, Jovanović, Marina, Jelenković, Ankica V., Bokonjić, D., Čolić, M., Stojanović, Ivana, Ninković, Milica, "Efekat L-NAME na AlCl3-indukovanu toksičnost u mozgu pacova" in Acta veterinaria, 59, no. 2-3 (2009):133-146,
https://doi.org/10.2298/AVB0903133S .,
https://hdl.handle.net/21.15107/rcub_ibiss_475 .

TY  - JOUR
AU  - Stojanović, Ivana
AU  - Jelenković, Ankica V.
AU  - Vasiljević, Ivana
AU  - Pavlović, Dušica
AU  - Bjelaković, Gordana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/40
AB  - In the CNS polyamines can exert opposite effects, depending on the concentration and conditions in the cell. Protective or neurotoxic polyamine effects were documented during seizures and repeated CNS excitation. Intensive research of exogenous polyamines effects during seizures induced by numerous agents did not clear up confusions about the duality of effects and the role of polyamines in seizures. In order to understand polyamine modulatory effects in seizures, the importance of NO and polyamine metabolism interdependence and the possible implication of changes of postulated NO and polyamine equillibrium in seizures, the effects of spermine alone and in combination with L-NAME (NOS inhibitor) on seizures induced by pentazol (PTZ) were investigated. To compare the obtained results, the effects of anticonvulsant midazolam on NO production during seizures were also investigated. Seizures were induced by i.p. application of pentazol (100 mg/kg b.w). Spermine and L-NAME were administered i.p. before PTZ. In the striatum and hippocampus, spermine induced increased NO production (p<0.001) related to values in the group treated by PTZ. Application of L-NAME before spermine and PTZ caused decrease of NO production in comparison with animals treated only by PTZ or spermine and PTZ. L-NAME given before spermine exerts protective effects related to seizures induced by PTZ and to the group treated by spermine, extending the time of seizure symptoms appearance, thus confirming the NO signaling system involvement in spermine effects during seizures. Highly significant PAO activity increase caused by spermine points out the intensified interconversion of spermine into putrescine, in order to maintain the intracellular putrescine concentration. The obtained results prove a strong relationship between the NO signaling system and polyamine metabolism in the brain during seizures and the importance of their changes in this kind of CNS injury.
AB  - U CNS-u poliamini mogu imati dijametralno suprotne efekte, zavisno od njihove koncentracije i uslova u ćeliji. U toku konvulzija i pri ponavljanoj ekscitaciji CNS-a dokazani su i protektivni i neurotoksični efekti poliamina. Intenzivna istraživanja efekata egzogenih poliamina u toku konvulzija izazvanih brojnim agensima in vivo nisu još uvek razjasnila nedoumice o dualitetu efekata i ulozi poliamina u konvulzijama. U cilju razjašnjenja modulatornih efekata poliamina u konvulzijama, značaja povezanosti metabolizma NO i poliamina i mogućnosti implikacije promena postulirane ravnoteže između NO i poliamina u konvulzijama, ispitivani su efekti spermina na konvulzije izazvane pentazolom (PTZ) i produkciju NO, kao i efekti spermina u kombinaciji sa L-NAME (inhibitorom NOS). Radi poređenja dobijenih rezultata, ispitivani su i efekti antikonvulziva midazolama na produkciju NO u toku konvulzija. Konvulzije su izazivane i.p. aplikacijom pentazola (100 mg/kg TM). Spermin i L-NAME su aplikovani i.p. pre PTZ-a. U strijatumu i hipokampusu spermin izaziva povećanu produkciju NO (p<0,001) u odnosu na vrednosti u grupi tretiranoj PTZom. Aplikacija L-NAME pre spermina i PTZ-a izazvala je smanjenje produkcije NO u odnosu na životinje tretirane samo PTZ-om ili sperminom i PTZ-om. Primena spermina pre pentazola produbljuje kliničke efekte pentazola. L-NAME, dat pre spermina, ispoljava protektivne efekte u odnosu na konvulzije izazvane pentazolom i grupu tretiranu sperminom, produžavajući vreme pojave simptoma konvulzija, što potvrđuje uključenost NO signalnog sistema u efekte spermina u toku konvulzivne aktivnosti. Visokosignifikantni porast aktivnosti PAO izazvan sperminom ukazuje na intenzivnu interkonverziju poliamina u putrescin u cilju održanja intracelularne koncentracije putrescina. Dobijeni rezultati dokazuju povezanost NO signalnog sistema i metabolizma poliamina i značaj njihovih promena u ovoj vrsti oštećenja CNS-a.
T2  - Journal of Medical Biochemistry
T1  - Uticaj spermina i L-name na metabolizam poliamina i azot monoksida u mozgu pacova u toku konvulzija
T1  - Spermine and L-name pretreatment effects on polyamine and nitric oxide metabolism in rat brain during seizures
IS  - 3
VL  - 26
SP  - 220
EP  - 226
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_40
ER  - 

@article{
author = "Stojanović, Ivana and Jelenković, Ankica V. and Vasiljević, Ivana and Pavlović, Dušica and Bjelaković, Gordana",
year = "2007, 2007",
abstract = "In the CNS polyamines can exert opposite effects, depending on the concentration and conditions in the cell. Protective or neurotoxic polyamine effects were documented during seizures and repeated CNS excitation. Intensive research of exogenous polyamines effects during seizures induced by numerous agents did not clear up confusions about the duality of effects and the role of polyamines in seizures. In order to understand polyamine modulatory effects in seizures, the importance of NO and polyamine metabolism interdependence and the possible implication of changes of postulated NO and polyamine equillibrium in seizures, the effects of spermine alone and in combination with L-NAME (NOS inhibitor) on seizures induced by pentazol (PTZ) were investigated. To compare the obtained results, the effects of anticonvulsant midazolam on NO production during seizures were also investigated. Seizures were induced by i.p. application of pentazol (100 mg/kg b.w). Spermine and L-NAME were administered i.p. before PTZ. In the striatum and hippocampus, spermine induced increased NO production (p<0.001) related to values in the group treated by PTZ. Application of L-NAME before spermine and PTZ caused decrease of NO production in comparison with animals treated only by PTZ or spermine and PTZ. L-NAME given before spermine exerts protective effects related to seizures induced by PTZ and to the group treated by spermine, extending the time of seizure symptoms appearance, thus confirming the NO signaling system involvement in spermine effects during seizures. Highly significant PAO activity increase caused by spermine points out the intensified interconversion of spermine into putrescine, in order to maintain the intracellular putrescine concentration. The obtained results prove a strong relationship between the NO signaling system and polyamine metabolism in the brain during seizures and the importance of their changes in this kind of CNS injury., U CNS-u poliamini mogu imati dijametralno suprotne efekte, zavisno od njihove koncentracije i uslova u ćeliji. U toku konvulzija i pri ponavljanoj ekscitaciji CNS-a dokazani su i protektivni i neurotoksični efekti poliamina. Intenzivna istraživanja efekata egzogenih poliamina u toku konvulzija izazvanih brojnim agensima in vivo nisu još uvek razjasnila nedoumice o dualitetu efekata i ulozi poliamina u konvulzijama. U cilju razjašnjenja modulatornih efekata poliamina u konvulzijama, značaja povezanosti metabolizma NO i poliamina i mogućnosti implikacije promena postulirane ravnoteže između NO i poliamina u konvulzijama, ispitivani su efekti spermina na konvulzije izazvane pentazolom (PTZ) i produkciju NO, kao i efekti spermina u kombinaciji sa L-NAME (inhibitorom NOS). Radi poređenja dobijenih rezultata, ispitivani su i efekti antikonvulziva midazolama na produkciju NO u toku konvulzija. Konvulzije su izazivane i.p. aplikacijom pentazola (100 mg/kg TM). Spermin i L-NAME su aplikovani i.p. pre PTZ-a. U strijatumu i hipokampusu spermin izaziva povećanu produkciju NO (p<0,001) u odnosu na vrednosti u grupi tretiranoj PTZom. Aplikacija L-NAME pre spermina i PTZ-a izazvala je smanjenje produkcije NO u odnosu na životinje tretirane samo PTZ-om ili sperminom i PTZ-om. Primena spermina pre pentazola produbljuje kliničke efekte pentazola. L-NAME, dat pre spermina, ispoljava protektivne efekte u odnosu na konvulzije izazvane pentazolom i grupu tretiranu sperminom, produžavajući vreme pojave simptoma konvulzija, što potvrđuje uključenost NO signalnog sistema u efekte spermina u toku konvulzivne aktivnosti. Visokosignifikantni porast aktivnosti PAO izazvan sperminom ukazuje na intenzivnu interkonverziju poliamina u putrescin u cilju održanja intracelularne koncentracije putrescina. Dobijeni rezultati dokazuju povezanost NO signalnog sistema i metabolizma poliamina i značaj njihovih promena u ovoj vrsti oštećenja CNS-a.",
journal = "Journal of Medical Biochemistry",
title = "Uticaj spermina i L-name na metabolizam poliamina i azot monoksida u mozgu pacova u toku konvulzija, Spermine and L-name pretreatment effects on polyamine and nitric oxide metabolism in rat brain during seizures",
number = "3",
volume = "26",
pages = "220-226",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_40"
}

Stojanović, I., Jelenković, A. V., Vasiljević, I., Pavlović, D.,& Bjelaković, G.. (2007). Uticaj spermina i L-name na metabolizam poliamina i azot monoksida u mozgu pacova u toku konvulzija. in Journal of Medical Biochemistry, 26(3), 220-226.
https://hdl.handle.net/21.15107/rcub_ibiss_40

Stojanović I, Jelenković AV, Vasiljević I, Pavlović D, Bjelaković G. Uticaj spermina i L-name na metabolizam poliamina i azot monoksida u mozgu pacova u toku konvulzija. in Journal of Medical Biochemistry. 2007;26(3):220-226.
https://hdl.handle.net/21.15107/rcub_ibiss_40 .

Stojanović, Ivana, Jelenković, Ankica V., Vasiljević, Ivana, Pavlović, Dušica, Bjelaković, Gordana, "Uticaj spermina i L-name na metabolizam poliamina i azot monoksida u mozgu pacova u toku konvulzija" in Journal of Medical Biochemistry, 26, no. 3 (2007):220-226,
https://hdl.handle.net/21.15107/rcub_ibiss_40 .