TY  - JOUR
AU  - Radojević, Anđelina
AU  - Mirčić, Dejan
AU  - Živić, Miroslav
AU  - Perić Mataruga, Vesna
AU  - Božanić, Milenka
AU  - Stojanović, Katarina
AU  - Lukičić, Jovana
AU  - Živić, Ivana
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900004R
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3771
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3399
AB  - The aim of the present study was to establish the extent to which the outlet waters of trout farms affect the ecosystems of the Crnica and Skrapež rivers in Serbia. We monitored selected biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and total glutathione (GSH) in larvae of the species Ecdyonurus venosus, and simultaneously analyzed the changes in the physical and chemical parameters. The investigations were carried out in spring at four localities along the Skrapež and Crnica rivers: one upstream (the control localities), and three downstream from the fish farm outlets. On the Skrapež River, the fish farm was clearly visible and was markedly changed by the chemical parameters of the water, manifested as a decreased concentration of dissolved oxygen, increased concentrations of nitrates, nitrites, ammonium ions, total phosphorus and total organic carbon, and increased SOD and GPx activities and decreased GSH concentration in larvae from the first downstream locality as compared to the control locality. On the Crnica River, due to the high values of water flow (around 3 m3/s), effluents from the fish farm had no effect on the chemical parameters of the water or on the tested biomarkers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)
IS  - 2
VL  - 71
DO  - 10.2298/ABS181220004R
SP  - 225
EP  - 233
ER  - 

@article{
author = "Radojević, Anđelina and Mirčić, Dejan and Živić, Miroslav and Perić Mataruga, Vesna and Božanić, Milenka and Stojanović, Katarina and Lukičić, Jovana and Živić, Ivana",
year = "2019",
abstract = "The aim of the present study was to establish the extent to which the outlet waters of trout farms affect the ecosystems of the Crnica and Skrapež rivers in Serbia. We monitored selected biomarkers of oxidative stress: superoxide dismutase (SOD), glutathione peroxidase (GPx) and total glutathione (GSH) in larvae of the species Ecdyonurus venosus, and simultaneously analyzed the changes in the physical and chemical parameters. The investigations were carried out in spring at four localities along the Skrapež and Crnica rivers: one upstream (the control localities), and three downstream from the fish farm outlets. On the Skrapež River, the fish farm was clearly visible and was markedly changed by the chemical parameters of the water, manifested as a decreased concentration of dissolved oxygen, increased concentrations of nitrates, nitrites, ammonium ions, total phosphorus and total organic carbon, and increased SOD and GPx activities and decreased GSH concentration in larvae from the first downstream locality as compared to the control locality. On the Crnica River, due to the high values of water flow (around 3 m3/s), effluents from the fish farm had no effect on the chemical parameters of the water or on the tested biomarkers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)",
number = "2",
volume = "71",
doi = "10.2298/ABS181220004R",
pages = "225-233"
}

Radojević, A., Mirčić, D., Živić, M., Perić Mataruga, V., Božanić, M., Stojanović, K., Lukičić, J.,& Živić, I.. (2019). Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae). in Archives of Biological Sciences
Serbian Biological Society., 71(2), 225-233.
https://doi.org/10.2298/ABS181220004R

Radojević A, Mirčić D, Živić M, Perić Mataruga V, Božanić M, Stojanović K, Lukičić J, Živić I. Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae). in Archives of Biological Sciences. 2019;71(2):225-233.
doi:10.2298/ABS181220004R .

Radojević, Anđelina, Mirčić, Dejan, Živić, Miroslav, Perić Mataruga, Vesna, Božanić, Milenka, Stojanović, Katarina, Lukičić, Jovana, Živić, Ivana, "Influence of trout farm effluents on selected oxidative stress biomarkers in larvae of Ecdyonurus venosus (Ephemeroptera, Heptageniidae)" in Archives of Biological Sciences, 71, no. 2 (2019):225-233,
https://doi.org/10.2298/ABS181220004R . .

TY  - CONF
AU  - Božanić, Milenka
AU  - Todorović, Dajana
AU  - Radojević, Anđelina
AU  - Perić Mataruga, Vesna
AU  - Živić, Miroslav
AU  - Stojanović, Katarina
AU  - Živić, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4022
AB  - Za analizu aktivnosti superoksid dismutaze (SOD), pod uticajem ispusnih voda pastrmskog ribnjaka u reci Skrapez, koriscena je larva vrste Ephemera danica kao model organizam. Istrazivanja su obavljena sezonski tokom 2015. i 2016. godine, na cetiri lokaliteta, jedan iznad, (SKI, kontrolni lokalitet) i tri ispod (SK2, SK3 i SK4) pastrmskog ribnjaka. Za analizu podataka koriscena je analiza koinercije (CIA). Od hemijskih i fizickih parametara analizirani su: temperatura, brzina vode, rastvoreni kiseonik, ukupni azot, amonijak, nitriti, nitrati i fosfati. Aktivnost SOD karakterise veca sezonska nego longitudinalna varijabilnost. CIA analiza pokazuje da je Fl osa, osa sezonskih promena, a enzim SOD se nalazi na njenom negativnom kraju. SOD karakterise statisticki znacajna korelacija sa rastvorenim kiseonikom (R=0,714, P=0,002), ukupnim azotom (R=0,592, P=0,016) i temperaturom (R=0,834, P<0,001). U odnosu na kontrolni lokalitet dolazi do opadanja aktivnosti SOD od proleca do zime. Povecana aktivnost ovog enzima u prolece i leto je posledica povisene temperature, sa kojom je SOD korelisan, kao i vece metabolicke aktivnosti organizma. Na aktivnost SOD, snazan uticaj ima promena faktora unutrasnje i spoljasnje sredine uslovljena smenom godisnjih doba, posto su kako u odnosu na amplitudu aktivnosti, tako i na obrazac njihovih sezonskih promena lokaliteti nizvodno od ispusta ribnjaka veoma slicni kontrolnom lokalitetu. Izuzetak je jesen, kada aktivnost SOD dostize maksimum na SK2. To moze biti posledica koncentracije kiseonika koja upravo u jesen na ovom lokalitetu ima najnizu vrednost (8,06 mg/I). Na osnovu dobijenih rezultata, zakljucuje se da ispusne vode pastrmskog ribnjaka izazivaju stres kod vrste E. danica sto se reflektuje kroz izmenjenu aktivnost SOD.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije
T1  - Aktivnost superoksid dismutaze larve Ephemera danica - biomarker oksidativnog stresa izazvang otpadnim vodama pastrmskih ribnjaka
SP  - 309
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4022
ER  - 

@conference{
author = "Božanić, Milenka and Todorović, Dajana and Radojević, Anđelina and Perić Mataruga, Vesna and Živić, Miroslav and Stojanović, Katarina and Živić, Ivana",
year = "2018",
abstract = "Za analizu aktivnosti superoksid dismutaze (SOD), pod uticajem ispusnih voda pastrmskog ribnjaka u reci Skrapez, koriscena je larva vrste Ephemera danica kao model organizam. Istrazivanja su obavljena sezonski tokom 2015. i 2016. godine, na cetiri lokaliteta, jedan iznad, (SKI, kontrolni lokalitet) i tri ispod (SK2, SK3 i SK4) pastrmskog ribnjaka. Za analizu podataka koriscena je analiza koinercije (CIA). Od hemijskih i fizickih parametara analizirani su: temperatura, brzina vode, rastvoreni kiseonik, ukupni azot, amonijak, nitriti, nitrati i fosfati. Aktivnost SOD karakterise veca sezonska nego longitudinalna varijabilnost. CIA analiza pokazuje da je Fl osa, osa sezonskih promena, a enzim SOD se nalazi na njenom negativnom kraju. SOD karakterise statisticki znacajna korelacija sa rastvorenim kiseonikom (R=0,714, P=0,002), ukupnim azotom (R=0,592, P=0,016) i temperaturom (R=0,834, P<0,001). U odnosu na kontrolni lokalitet dolazi do opadanja aktivnosti SOD od proleca do zime. Povecana aktivnost ovog enzima u prolece i leto je posledica povisene temperature, sa kojom je SOD korelisan, kao i vece metabolicke aktivnosti organizma. Na aktivnost SOD, snazan uticaj ima promena faktora unutrasnje i spoljasnje sredine uslovljena smenom godisnjih doba, posto su kako u odnosu na amplitudu aktivnosti, tako i na obrazac njihovih sezonskih promena lokaliteti nizvodno od ispusta ribnjaka veoma slicni kontrolnom lokalitetu. Izuzetak je jesen, kada aktivnost SOD dostize maksimum na SK2. To moze biti posledica koncentracije kiseonika koja upravo u jesen na ovom lokalitetu ima najnizu vrednost (8,06 mg/I). Na osnovu dobijenih rezultata, zakljucuje se da ispusne vode pastrmskog ribnjaka izazivaju stres kod vrste E. danica sto se reflektuje kroz izmenjenu aktivnost SOD.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije",
title = "Aktivnost superoksid dismutaze larve Ephemera danica - biomarker oksidativnog stresa izazvang otpadnim vodama pastrmskih ribnjaka",
pages = "309",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4022"
}

Božanić, M., Todorović, D., Radojević, A., Perić Mataruga, V., Živić, M., Stojanović, K.,& Živić, I.. (2018). Aktivnost superoksid dismutaze larve Ephemera danica - biomarker oksidativnog stresa izazvang otpadnim vodama pastrmskih ribnjaka. in Drugi kongres biologa Srbije
Belgrade: Serbian Biological Society., 309.
https://hdl.handle.net/21.15107/rcub_ibiss_4022

Božanić M, Todorović D, Radojević A, Perić Mataruga V, Živić M, Stojanović K, Živić I. Aktivnost superoksid dismutaze larve Ephemera danica - biomarker oksidativnog stresa izazvang otpadnim vodama pastrmskih ribnjaka. in Drugi kongres biologa Srbije. 2018;:309.
https://hdl.handle.net/21.15107/rcub_ibiss_4022 .

Božanić, Milenka, Todorović, Dajana, Radojević, Anđelina, Perić Mataruga, Vesna, Živić, Miroslav, Stojanović, Katarina, Živić, Ivana, "Aktivnost superoksid dismutaze larve Ephemera danica - biomarker oksidativnog stresa izazvang otpadnim vodama pastrmskih ribnjaka" in Drugi kongres biologa Srbije (2018):309,
https://hdl.handle.net/21.15107/rcub_ibiss_4022 .

TY  - JOUR
AU  - Vranković, Jelena
AU  - Živić, Miroslav
AU  - Radojević, Anđelina
AU  - Perić Mataruga, Vesna
AU  - Todorović, Dajana
AU  - Marković, Zoran
AU  - Živić, Ivana
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0147651318306560?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3114
AB  - The current study reports a combined seasonal monitoring of the Crnica River watercourse using physico-chemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.
PB  - Academic Press
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs
VL  - 163
DO  - 10.1016/J.ECOENV.2018.07.061
SP  - 84
EP  - 95
ER  - 

@article{
author = "Vranković, Jelena and Živić, Miroslav and Radojević, Anđelina and Perić Mataruga, Vesna and Todorović, Dajana and Marković, Zoran and Živić, Ivana",
year = "2018",
abstract = "The current study reports a combined seasonal monitoring of the Crnica River watercourse using physico-chemical parameters and biomarkers measured in Gammarus dulensis with the aim of correlating the effects of pollution with the level of oxidative stress biomarkers in this amphipod. The pollution source is identified as a trout farm in the upper part of the Crnica River (Eastern Serbia). Physico-chemical water parameters and sediment trace element concentrations were measured in the Crnica River during three seasons. Three localities downstream [CR2, CR3, and CR4 (20 m, 400 m, and 1.3 km from the trout farm discharge, respectively)] were assessed in comparison with a reference location (CR1), 250 m upstream from the trout aquaculture in the Crnica River. Antioxidant biomarkers including the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione-S-transferase (GST) were determined in pooled samples of G. dulensis. Our findings showed statistically different antioxidant enzyme levels in gammarids from the first downstream locality (CR2) compared to those from other three localities. The results indicate that oxidative stress occurred to some extent despite the assumed antioxidant induction as a result of exposure to elevated levels of some elements and changed water parameters, the effects seeming in part to be mediated by suppression of SOD and CAT (during summer and autumn); and by suppression of GPX and GR (during autumn) at the CR2 locality. The activity of GST in all seasons was higher at the CR2 locality than at the other localities, suggesting the presence of some permanent xenobiotic next to the aquaculture output. Seasonal difference in the physical and chemical water parameters and sediment trace element concentrations were observed. Overall, our results indicate a locality- and season-dependent antioxidant enzymes response in G. dulensis, pointing at progressively reduced deleterious effect of trout farm outputs with movement further downstream.",
publisher = "Academic Press",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs",
volume = "163",
doi = "10.1016/J.ECOENV.2018.07.061",
pages = "84-95"
}

Vranković, J., Živić, M., Radojević, A., Perić Mataruga, V., Todorović, D., Marković, Z.,& Živić, I.. (2018). Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety
Academic Press., 163, 84-95.
https://doi.org/10.1016/J.ECOENV.2018.07.061

Vranković J, Živić M, Radojević A, Perić Mataruga V, Todorović D, Marković Z, Živić I. Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs. in Ecotoxicology and Environmental Safety. 2018;163:84-95.
doi:10.1016/J.ECOENV.2018.07.061 .

Vranković, Jelena, Živić, Miroslav, Radojević, Anđelina, Perić Mataruga, Vesna, Todorović, Dajana, Marković, Zoran, Živić, Ivana, "Evaluation of oxidative stress biomarkers in the freshwater gammarid Gammarus dulensis exposed to trout farm outputs" in Ecotoxicology and Environmental Safety, 163 (2018):84-95,
https://doi.org/10.1016/J.ECOENV.2018.07.061 . .