TY  - CONF
AU  - Vidović, Biljana
AU  - Weyl, Philip
AU  - Marini, Francesca
AU  - Cristofaro, Massimo
AU  - Cvrković, Tatjana
AU  - Jojić, Vida
AU  - Petanović, Radmila
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5639
AB  - In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.
PB  - Belgrade: Plant Protection Society of Serbia
C3  - Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
T1  - Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5639
ER  - 

@conference{
author = "Vidović, Biljana and Weyl, Philip and Marini, Francesca and Cristofaro, Massimo and Cvrković, Tatjana and Jojić, Vida and Petanović, Radmila",
year = "2019",
abstract = "In the past few decades, increasing interest in the use of eriophyoid mites as biological control agents has been expressed, especially due to their high host specificity, high intrinsic rate of reproduction and damage frequently caused to reproductive plant parts. To date, three Metaculus species were registered as potential biological control agents and the necessity of more comprehensive understanding of taxonomic status of Metaculus rapistri has been emphasized (Vidović et al, 2015). Namely, M. rapistri was initially described by Carmona (1969) from samples of R. rugosum collected in Portugal. Thereafter, a supplementary morphological description of this species was provided by Monfreda and De Lillo (2012) from samples of Isatis tinctoria collected in Turkey. The goal of this study was to clarify the taxonomic status of mite species within the genus Metaculus that inhabit R. rugosum and I. tinctoria. 
	Plant samples of R. rugosum were from Portugal, while I. tinctoria were collected from Turkey and Germany. For the morphometric study, 28–30 females from each sample were examined in the dorso-ventral position. Twenty-two morphological traits of each individual were measured. The multivariate analysis of variance (MANOVA) and Canonical variate analysis (CVA) were used to examine morphological differences among groups. 
	DNA was extracted from 30 to 40 whole specimens using QIAGEN DNeasy Blood & Tissue Kit. Mitochondrial COI sequences were amplified using primers LCO1490/HCO2198. Uncorrected pairwise genetic distances were used to calculate the average genetic divergence between species with different host affiliations. 
	As evident from the CVA plot, the CV1 axis (accounting for 79.2% of variance) separated M. rapistri hosted by R. rugosum from Metaculus spp. hosted by I. tinctoria, while CV2 axis (accounting for 20.8% of variance) discriminated Metaculus sp. from I. tinctoria collected in Germany from Metaculus sp. from I. tinctoria collected in Turkey. The barcoding region of mtCOI gene indicated a genetic divergence between the analyzed populations of M. rapistri from R. rugosum and M. rapistri from I. tinctoria (7.7–11%). The genetic divergence was registered also between populations of mites from I. tinctoria from Turkey and the same plant species from Germany (12.3%). The results of our study showed that M. rapistri from R. rugosum is a distinct species and that could be two different taxa hosted by I. tinctoria.",
publisher = "Belgrade: Plant Protection Society of Serbia",
journal = "Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia",
title = "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5639"
}

Vidović, B., Weyl, P., Marini, F., Cristofaro, M., Cvrković, T., Jojić, V.,& Petanović, R.. (2019). Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia
Belgrade: Plant Protection Society of Serbia., 165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639

Vidović B, Weyl P, Marini F, Cristofaro M, Cvrković T, Jojić V, Petanović R. Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae). in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia. 2019;:165.
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .

Vidović, Biljana, Weyl, Philip, Marini, Francesca, Cristofaro, Massimo, Cvrković, Tatjana, Jojić, Vida, Petanović, Radmila, "Morphological and molecular characterization of Metaculus eriophyid mites from Rapistrum rugosum and Isatis tinctoria (Brassicaceae)" in Book of abstracts: VIII Congress on Plant Protection: Integrated Plant Protection for Sustainable Crop Production and Forestry; 2019 Nov 25-29; Zlatibor, Serbia (2019):165,
https://hdl.handle.net/21.15107/rcub_ibiss_5639 .