TY  - JOUR
AU  - Protić, Isidora
AU  - Golić, Igor
AU  - Vidaković, Snežana
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2022
UR  - https://www.mdpi.com/1467-3045/44/8/237
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9406340
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5110
AB  - Zinc (in the form of Zn2+) is necessary for male fertility. Both Zn2+ quantity and its localisation have been detected in seminal plasma and ejaculated spermatozoa, suggesting its active uptake via zinc import transporters (ZIPs). Immunofluorescence was used to characterise the expression and localisation of three distinct types of ZIP transporters in ejaculated spermatozoa of normo- and asthenozoospermic sperm samples. ZIP6, ZIP10 and ZIP14 showed heterogeneous sperm cell expression and different compartmental distribution. In both types of sperm samples, ZIP6 and ZIP14 were predominantly localised in the sperm head, while ZIP10 was found along the sperm tail. Compartmental localisation of ZIPs in asthenozoospermia was not changed. However, regarding sub-compartmental localisation in sperm head regions, for ZIP6 asthenozoospermia only decreased its acorn/crescent-like pattern. In contrast, ZIP14 immunostaining was altered in favour of crescent-like, as opposed to acorn-like and acorn/crescent-like patterns. The specific ZIPs localisation may reflect their different roles in sperm cell integrity and motility and may change over time. This is the first report of their specific compartmental and sub-compartmental localisation in ejaculated human sperm cells. Further research will lead to a greater understanding of the roles of ZIPs in sperm cell biology, which could positively influence procedures for human infertility therapy.
PB  - Basel: MDPI
T2  - Current Issues in Molecular Biology
T1  - Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.
IS  - 8
VL  - 44
DO  - 10.3390/cimb44080237
SP  - 3444
EP  - 3454
ER  - 

@article{
author = "Protić, Isidora and Golić, Igor and Vidaković, Snežana and Korać, Bato and Korać, Aleksandra",
year = "2022",
abstract = "Zinc (in the form of Zn2+) is necessary for male fertility. Both Zn2+ quantity and its localisation have been detected in seminal plasma and ejaculated spermatozoa, suggesting its active uptake via zinc import transporters (ZIPs). Immunofluorescence was used to characterise the expression and localisation of three distinct types of ZIP transporters in ejaculated spermatozoa of normo- and asthenozoospermic sperm samples. ZIP6, ZIP10 and ZIP14 showed heterogeneous sperm cell expression and different compartmental distribution. In both types of sperm samples, ZIP6 and ZIP14 were predominantly localised in the sperm head, while ZIP10 was found along the sperm tail. Compartmental localisation of ZIPs in asthenozoospermia was not changed. However, regarding sub-compartmental localisation in sperm head regions, for ZIP6 asthenozoospermia only decreased its acorn/crescent-like pattern. In contrast, ZIP14 immunostaining was altered in favour of crescent-like, as opposed to acorn-like and acorn/crescent-like patterns. The specific ZIPs localisation may reflect their different roles in sperm cell integrity and motility and may change over time. This is the first report of their specific compartmental and sub-compartmental localisation in ejaculated human sperm cells. Further research will lead to a greater understanding of the roles of ZIPs in sperm cell biology, which could positively influence procedures for human infertility therapy.",
publisher = "Basel: MDPI",
journal = "Current Issues in Molecular Biology",
title = "Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.",
number = "8",
volume = "44",
doi = "10.3390/cimb44080237",
pages = "3444-3454"
}

Protić, I., Golić, I., Vidaković, S., Korać, B.,& Korać, A.. (2022). Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.. in Current Issues in Molecular Biology
Basel: MDPI., 44(8), 3444-3454.
https://doi.org/10.3390/cimb44080237

Protić I, Golić I, Vidaković S, Korać B, Korać A. Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.. in Current Issues in Molecular Biology. 2022;44(8):3444-3454.
doi:10.3390/cimb44080237 .

Protić, Isidora, Golić, Igor, Vidaković, Snežana, Korać, Bato, Korać, Aleksandra, "Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa." in Current Issues in Molecular Biology, 44, no. 8 (2022):3444-3454,
https://doi.org/10.3390/cimb44080237 . .

TY  - JOUR
AU  - Protić, Isidora
AU  - Golić, Igor
AU  - Aleksić, Marija
AU  - Vidaković, Snežana
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306987722000408
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4862
AB  - The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.
PB  - Edinburgh: Churchill Livingstone
T2  - Medical Hypotheses
T1  - Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity
VL  - 161
DO  - 10.1016/j.mehy.2022.110800
SP  - 110800
ER  - 

@article{
author = "Protić, Isidora and Golić, Igor and Aleksić, Marija and Vidaković, Snežana and Korać, Bato and Korać, Aleksandra",
year = "2022",
abstract = "The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.",
publisher = "Edinburgh: Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity",
volume = "161",
doi = "10.1016/j.mehy.2022.110800",
pages = "110800"
}

Protić, I., Golić, I., Aleksić, M., Vidaković, S., Korać, B.,& Korać, A.. (2022). Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses
Edinburgh: Churchill Livingstone., 161, 110800.
https://doi.org/10.1016/j.mehy.2022.110800

Protić I, Golić I, Aleksić M, Vidaković S, Korać B, Korać A. Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses. 2022;161:110800.
doi:10.1016/j.mehy.2022.110800 .

Protić, Isidora, Golić, Igor, Aleksić, Marija, Vidaković, Snežana, Korać, Bato, Korać, Aleksandra, "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity" in Medical Hypotheses, 161 (2022):110800,
https://doi.org/10.1016/j.mehy.2022.110800 . .