TY  - JOUR
AU  - Milinčić, Danijel D
AU  - Stanisavljević, Nemanja S
AU  - Kostić, Aleksandar Ž
AU  - Gašić, Uroš
AU  - Stanojević, Slađana P
AU  - Tešić, Živoslav Lj
AU  - Pešić, Mirjana B
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/11/2164
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5236
AB  - This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion
IS  - 11
VL  - 11
DO  - 10.3390/antiox11112164
SP  - 2164
ER  - 

@article{
author = "Milinčić, Danijel D and Stanisavljević, Nemanja S and Kostić, Aleksandar Ž and Gašić, Uroš and Stanojević, Slađana P and Tešić, Živoslav Lj and Pešić, Mirjana B",
year = "2022",
abstract = "This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion",
number = "11",
volume = "11",
doi = "10.3390/antiox11112164",
pages = "2164"
}

Milinčić, D. D., Stanisavljević, N. S., Kostić, A. Ž., Gašić, U., Stanojević, S. P., Tešić, Ž. L.,& Pešić, M. B.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants
Basel: MDPI., 11(11), 2164.
https://doi.org/10.3390/antiox11112164

Milinčić DD, Stanisavljević NS, Kostić AŽ, Gašić U, Stanojević SP, Tešić ŽL, Pešić MB. Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants. 2022;11(11):2164.
doi:10.3390/antiox11112164 .

Milinčić, Danijel D, Stanisavljević, Nemanja S, Kostić, Aleksandar Ž, Gašić, Uroš, Stanojević, Slađana P, Tešić, Živoslav Lj, Pešić, Mirjana B, "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion" in Antioxidants, 11, no. 11 (2022):2164,
https://doi.org/10.3390/antiox11112164 . .

TY  - JOUR
AU  - Katanić Stanković, Jelena S.
AU  - Mićanović, Nenad
AU  - Grozdanić, Nađa
AU  - Kostić, Aleksandar Ž.
AU  - Gašić, Uroš
AU  - Stanojković, Tatjana
AU  - Popović-Đorđević, Jelena B.
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/11/1053
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5241
AB  - Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia
IS  - 11
VL  - 8
DO  - 10.3390/horticulturae8111053
SP  - 1053
ER  - 

@article{
author = "Katanić Stanković, Jelena S. and Mićanović, Nenad and Grozdanić, Nađa and Kostić, Aleksandar Ž. and Gašić, Uroš and Stanojković, Tatjana and Popović-Đorđević, Jelena B.",
year = "2022",
abstract = "Plant-based food represents an excellent source of different nutrients and bioactive compounds, such as phenolics, carotenoids, vitamins, etc., with proven health benefits for humans. The content of selected phytochemicals, polyphenolic profile, and biological activity (antioxidant potential and α-glucosidase inhibitory activity) of fruit extracts of medlar (Mespilus germanica L.), blackthorn (Prunus spinosa L.), and common hawthorn (Crataegus monogyna Jacq.), the neglected Rosaceae species originated from Serbia were studied. Targeted UHPLC/(−)HESI–MS/MS quantitative analysis of phenolic compounds revealed pinocembrin only in medlar fruit extract, and it is the first report of this flavanone in medlar fruits. Total phenolic content did not differ between extracts, whereas significant differences were observed for the contents of total flavonoids, total phenolic acids, and total gallotannins. Monomeric anthocyanins and total anthocyanins were significantly higher in blackthorn compared to medlar and hawthorn fruit extracts (p < 0.05). DPPH· and ABTS·+ scavenging activities for examined fruits were modest compared to other natural antioxidants and BHT. The most potent inhibitory activity toward α-glucosidase expressed medlar and blackthorn extracts with IC50 values of 129.46 and 199.84 μg/mL, respectively, which was higher compared to the standard drug acarbose.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia",
number = "11",
volume = "8",
doi = "10.3390/horticulturae8111053",
pages = "1053"
}

Katanić Stanković, J. S., Mićanović, N., Grozdanić, N., Kostić, A. Ž., Gašić, U., Stanojković, T.,& Popović-Đorđević, J. B.. (2022). Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae
Basel: MDPI., 8(11), 1053.
https://doi.org/10.3390/horticulturae8111053

Katanić Stanković JS, Mićanović N, Grozdanić N, Kostić AŽ, Gašić U, Stanojković T, Popović-Đorđević JB. Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia. in Horticulturae. 2022;8(11):1053.
doi:10.3390/horticulturae8111053 .

Katanić Stanković, Jelena S., Mićanović, Nenad, Grozdanić, Nađa, Kostić, Aleksandar Ž., Gašić, Uroš, Stanojković, Tatjana, Popović-Đorđević, Jelena B., "Polyphenolic Profile, Antioxidant and Antidiabetic Potential of Medlar (Mespilus germanica L.), Blackthorn (Prunus spinosa L.) and Common Hawthorn (Crataegus monogyna Jacq.) Fruit Extracts from Serbia" in Horticulturae, 8, no. 11 (2022):1053,
https://doi.org/10.3390/horticulturae8111053 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Mačukanović-Jocić, Marina P
AU  - Milinčić, Danijel D.
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Gligorijević, Nevenka N.
AU  - Jarić, Snežana
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200069
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35213785
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4941
AB  - The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.
T2  - Chemistry & Biodiversity
T1  - Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.
IS  - 4
VL  - 19
DO  - 10.1002/cbdv.202200069
SP  - e202200069
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Mačukanović-Jocić, Marina P and Milinčić, Danijel D. and Petrović, Jovana and Gašić, Uroš and Gligorijević, Nevenka N. and Jarić, Snežana and Soković, Marina and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.",
journal = "Chemistry & Biodiversity",
title = "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.",
number = "4",
volume = "19",
doi = "10.1002/cbdv.202200069",
pages = "e202200069"
}

Kostić, A. Ž., Mačukanović-Jocić, M. P., Milinčić, D. D., Petrović, J., Gašić, U., Gligorijević, N. N., Jarić, S., Soković, M., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity, 19(4), e202200069.
https://doi.org/10.1002/cbdv.202200069

Kostić AŽ, Mačukanović-Jocić MP, Milinčić DD, Petrović J, Gašić U, Gligorijević NN, Jarić S, Soković M, Tešić ŽL, Pešić MB. Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity. 2022;19(4):e202200069.
doi:10.1002/cbdv.202200069 .

Kostić, Aleksandar Ž., Mačukanović-Jocić, Marina P, Milinčić, Danijel D., Petrović, Jovana, Gašić, Uroš, Gligorijević, Nevenka N., Jarić, Snežana, Soković, Marina, Tešić, Živoslav Lj., Pešić, Mirjana B., "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents." in Chemistry & Biodiversity, 19, no. 4 (2022):e202200069,
https://doi.org/10.1002/cbdv.202200069 . .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Milinčić, Danijel D.
AU  - Stanisavljević, Nemanja S.
AU  - Gašić, Uroš
AU  - Lević, Steva
AU  - Kojić, Milan O.
AU  - Lj Tešić, Živoslav
AU  - Nedović, Viktor
AU  - Barać, Miroljub B.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814621003162
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33640767
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4158
AB  - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7-76.2%) followed by phenolic acids (14.2-17.4%). Among individual compounds quercetin-3-O-glucoside (155.1-197.2 μg/L) and p-coumaric acid (29.5-30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
PB  - Elsevier
T2  - Food Chemistry
T1  - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen.
VL  - 351
DO  - 10.1016/j.foodchem.2021.129310
SP  - 129310
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Gašić, Uroš and Lević, Steva and Kojić, Milan O. and Lj Tešić, Živoslav and Nedović, Viktor and Barać, Miroljub B. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7-76.2%) followed by phenolic acids (14.2-17.4%). Among individual compounds quercetin-3-O-glucoside (155.1-197.2 μg/L) and p-coumaric acid (29.5-30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen.",
volume = "351",
doi = "10.1016/j.foodchem.2021.129310",
pages = "129310"
}

Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U., Lević, S., Kojić, M. O., Lj Tešić, Ž., Nedović, V., Barać, M. B.,& Pešić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen.. in Food Chemistry
Elsevier., 351, 129310.
https://doi.org/10.1016/j.foodchem.2021.129310

Kostić AŽ, Milinčić DD, Stanisavljević NS, Gašić U, Lević S, Kojić MO, Lj Tešić Ž, Nedović V, Barać MB, Pešić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen.. in Food Chemistry. 2021;351:129310.
doi:10.1016/j.foodchem.2021.129310 .

Kostić, Aleksandar Ž., Milinčić, Danijel D., Stanisavljević, Nemanja S., Gašić, Uroš, Lević, Steva, Kojić, Milan O., Lj Tešić, Živoslav, Nedović, Viktor, Barać, Miroljub B., Pešić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen." in Food Chemistry, 351 (2021):129310,
https://doi.org/10.1016/j.foodchem.2021.129310 . .

TY  - JOUR
AU  - Kolarević, Tijana
AU  - Milinčić, Danijel D.
AU  - Vujović, Tatjana
AU  - Gašić, Uroš
AU  - Prokić, Ljiljana
AU  - Kostić, Aleksandar Ž.
AU  - Cerović, Radosav
AU  - Stanojević, Slađana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://www.mdpi.com/2311-7524/7/11/420
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4665
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
VL  - 7
DO  - 10.3390/horticulturae7110420
SP  - 420
ER  - 

@article{
author = "Kolarević, Tijana and Milinčić, Danijel D. and Vujović, Tatjana and Gašić, Uroš and Prokić, Ljiljana and Kostić, Aleksandar Ž. and Cerović, Radosav and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
volume = "7",
doi = "10.3390/horticulturae7110420",
pages = "420"
}

Kolarević, T., Milinčić, D. D., Vujović, T., Gašić, U., Prokić, L., Kostić, A. Ž., Cerović, R., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae
Basel: MDPI., 7(11), 420.
https://doi.org/10.3390/horticulturae7110420

Kolarević T, Milinčić DD, Vujović T, Gašić U, Prokić L, Kostić AŽ, Cerović R, Stanojević SP, Tešić ŽL, Pešić MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11):420.
doi:10.3390/horticulturae7110420 .

Kolarević, Tijana, Milinčić, Danijel D., Vujović, Tatjana, Gašić, Uroš, Prokić, Ljiljana, Kostić, Aleksandar Ž., Cerović, Radosav, Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021):420,
https://doi.org/10.3390/horticulturae7110420 . .

TY  - JOUR
AU  - Stikić, Radmila I.
AU  - Milinčić, Danijel D.
AU  - Kostić, Aleksandar Ž.
AU  - Jovanović, Zorica B.
AU  - Gašić, Uroš
AU  - Tešić, Živoslav Lj.
AU  - Djordjević, Nataša Z.
AU  - Savić, Sladjana K.
AU  - Czekus, Borisz G.
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cche.10278
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3639
AB  - Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
IS  - 3
VL  - 97
DO  - 10.1002/cche.10278
SP  - cche.10278
SP  - 626
EP  - 633
ER  - 

@article{
author = "Stikić, Radmila I. and Milinčić, Danijel D. and Kostić, Aleksandar Ž. and Jovanović, Zorica B. and Gašić, Uroš and Tešić, Živoslav Lj. and Djordjević, Nataša Z. and Savić, Sladjana K. and Czekus, Borisz G. and Pešić, Mirjana",
year = "2020",
abstract = "Quinoa is considered to be a “natural functional food” due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health‐promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. 13 phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration, and time‐dependent effects were more expressed in Puno extracts. The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT‐116, as well as the presence of 7 new phenolic and flavonoid compounds.",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
number = "3",
volume = "97",
doi = "10.1002/cche.10278",
pages = "cche.10278-626-633"
}

Stikić, R. I., Milinčić, D. D., Kostić, A. Ž., Jovanović, Z. B., Gašić, U., Tešić, Ž. Lj., Djordjević, N. Z., Savić, S. K., Czekus, B. G.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry, 97(3), cche.10278-633.
https://doi.org/10.1002/cche.10278

Stikić RI, Milinčić DD, Kostić AŽ, Jovanović ZB, Gašić U, Tešić ŽL, Djordjević NZ, Savić SK, Czekus BG, Pešić M. Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):cche.10278-633.
doi:10.1002/cche.10278 .

Stikić, Radmila I., Milinčić, Danijel D., Kostić, Aleksandar Ž., Jovanović, Zorica B., Gašić, Uroš, Tešić, Živoslav Lj., Djordjević, Nataša Z., Savić, Sladjana K., Czekus, Borisz G., Pešić, Mirjana, "Polyphenolic profiles, antioxidant and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):cche.10278-633,
https://doi.org/10.1002/cche.10278 . .